MD06G1202200.v1.1

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001632
Physical Location & Seq
Forward (+)
33544265 .. 33550767
6503 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1202200.v1.1.491

Sequence Viewer

Length: 1818 bp
ATGCAAGAACCGAAAGAGACCCACATGGTCAAACCCTGTGATACATGTGGTGGTGGTAACGACGAGAACCTCATTGCAACCTGCCGTGAATGCAATATAGCTCGTGCACACCCTTATTGCATGCAGAAACTTGATACGGAAGCTTGGGACGATTGGGTTTGCGAAGAATGTCTTGGAGAGGAAAACAATTTAGCATCCCCCACCGATTTAGACCACCATGGTATCATGCATCCATCAGGTAACAGCAGAGTTCTTGATAACTCGGGGTGGCAAGTTCATTCTAGAAGGCAAAAGCCGGTTGGAACTGGAAAAGTTAAGTACATTTCAGAAGGAGAAGTCATAAGGCTATCATCAGGAGTTGAAATAACGAGATCACAGCGAAATACTTTCGGCTTCAAACCTGGTCAGTCAAATTCTACAGCACTTATGTCCCAAAGGACTACTTTTGGCTCTAAAAGTGTGATACCGAGGTCTCCACCGCCTGCAATCAAAGGAAATCCTAGCATTGTGGCTTCAGGACATGTGAAGCCTCCTAGCTGTGGCAGGAATATCAAGATGTCAAGCATCTCAAAGATAAATCAGCAAACATCTCCAATATTGAAGGATTCAAAAGAACCAAAGGCTCCTTTTGCTCAGAACTCAAAGAAATATATTTCCAGTGATCACCGAGTAGTTGCAACCTCGCCTGTTAAAGAAGCCAAATCATCAAAGCTCAGTATACACAGTCGGATAACGAATCCAAAAACAAAGACTTCAGTAGCTTATGGAAAGGAGCATATTCGGGAGAAGCAATCAAAGGATGTCCTAGTTCCAGCAACATCAATTGAAACAAAGAACCCAGTAGCTCCTGGAGAGGAGCATGTTCGGGAAAAGCAACCACAAGATGTTTTAATTCCAAGACCAGATGTAACTTCTCGGAAGAAGAGGGTTACTGCAATTGAAAAGGAGCCGTGCACAGTTTCAACAATGAGGCCCTCCTCACCTATGTCCAGTCCAGAACATAACCATGCAGATGCTGAAGAAAGAGATCTCGAGAATAAGCTTGCTAGTCTCAATCTAAATCACTCAAGTCTTCCTGCTCTACAAGTCACTTGGAGGGGAGGCTTCATTGTTGATACAGCTACAGCTAATGAATTCATCAGAGGGTTCCAGGGACGACCTCCATGTAAGATCCATCCTAAAGCCTATAGATTTGCACAAACAATGCCTCCTGTCCTCAAGGCGAATTTTATTCCTCGATCCCAACTTTGGACTGATATATTCCAAGACCAACCTCCTGACCTTCAAGATGTTGGAATTTACTTTTTCCCTGATAGAAATATTGAGAGATCAAGAGAGAACCATGCTCGGCTGATTGAGCGGATGGAGAAAGAAGATTCGATGATGAGAATTTGCTTTGACGACCAGGGCGTGGAGTTGCTAATATTTACATCAGAACAGCTACAGTTAGACACGCAGTTGACAAGCTTCTTATGGGGAATCTTTCATTGTACGAAAAACTATCAAGTTGAAGCAAACATGGTTGACAATCAGAGTGTGGACATGGAAATCGACATGGAAGGTGGAAATATGGTGGGAAGAGTTGATGTTGTGATTCAGAGGGATCCAAAGGTTACTAGAAAAGGCAAAACATCATCAGTGAAAACCAGATATGGCCCGCGATCAAACTCGAGGAATGAAGGTGGTGAAATCTCCAGTGATGCAGAGAAGGAAGAATTGGCACGTCCACCGGGTTTTAGTGAGGGCAGTCCTCAGAGTGCTACTGGTTCAACTGAGGGAATGGCAGGGCTGTCTTCATCTTTTCGACGCAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

606

Amino Acids

67.4

Weight (kDa)

8.58

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC_AIPP2 PF23121 365 - 495 1.6e-35 AIPP2, SPOC-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 1411
AccBSI CCGCTC 1 cut(s) 1360
AccI GTMKAC 1 cut(s) 718
AccII CGCG 1 cut(s) 1660
AciI CCGC 3 cut(s) 479, 1360, 1658
AclWI GGATC 4 cut(s) 1165, 1233, 1598, 1611
AcsI RAATTY 5 cut(s) 412, 1133, 1225, 1296, 1389
AcuI CTGAAG 3 cut(s) 498, 738, 1038
AfaI GTAC 2 cut(s) 320, 1492
AfiI CCNNNNNNNGG 3 cut(s) 539, 1248, 1411
AflIII ACRYGT 2 cut(s) 44, 520
AjiI CACGTC 1 cut(s) 1724
AjnI CCWGG 4 cut(s) 400, 847, 1149, 1404
AjuI GAANNNNNNNTTGG 4 cut(s) 156, 188, 1700, 1732
Alw21I GWGCWC 2 cut(s) 109, 956
Alw26I GTCTC 3 cut(s) 11, 477, 1055
Alw44I GTGCAC 2 cut(s) 105, 952
AlwI GGATC 4 cut(s) 1165, 1233, 1598, 1611
AlwNI CAGNNNCTG 1 cut(s) 1016
Ama87I CYCGRG 3 cut(s) 262, 1031, 1669
AoxI GGCC 2 cut(s) 971, 1654
ApaLI GTGCAC 2 cut(s) 105, 952
ApoI RAATTY 5 cut(s) 412, 1133, 1225, 1296, 1389
AspS9I GGNCC 2 cut(s) 972, 1655
AsuC2I CCSGG 1 cut(s) 1731
AsuHPI GGTGA 3 cut(s) 656, 972, 1697
AvaI CYCGRG 3 cut(s) 262, 1031, 1669
BaeGI GKGCMC 2 cut(s) 109, 956
BaeI ACNNNNGTAYC 4 cut(s) 205, 238, 455, 488
BamHI GGATCC 1 cut(s) 1603
BauI CACGAG 1 cut(s) 102
BbsI GAAGAC 2 cut(s) 1064, 1785
Bbv12I GWGCWC 2 cut(s) 109, 956
BccI CCATC 3 cut(s) 241, 1182, 1357
BceAI ACGGC 2 cut(s) 69, 934
BciT130I CCWGG 4 cut(s) 402, 849, 1151, 1406
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 1 cut(s) 1731
BcoDI GTCTC 3 cut(s) 11, 477, 1055
BfaI CTAG 6 cut(s) 282, 501, 534, 806, 1047, 1617
BfmI CTRYAG 4 cut(s) 417, 1122, 1186, 1442
BfuAI ACCTGC 1 cut(s) 89
BglII AGATCT 1 cut(s) 1027
Bme1390I CCNGG 5 cut(s) 402, 849, 1151, 1406, 1731
BmeT110I CYCGRG 3 cut(s) 262, 1031, 1669
BmgBI CACGTC 1 cut(s) 1724
BmgT120I GGNCC 2 cut(s) 972, 1655
BmiI GGNNCC 4 cut(s) 624, 948, 1148, 1605
BmrFI CCNGG 5 cut(s) 402, 849, 1151, 1406, 1731
BmrI ACTGGG 1 cut(s) 833
BmsI GCATC 5 cut(s) 203, 238, 573, 1003, 1690
BmuI ACTGGG 1 cut(s) 833
BpiI GAAGAC 2 cut(s) 1064, 1785
BpmI CTGGAG 2 cut(s) 870, 1678
BpuEI CTTGAG 2 cut(s) 1051, 1202
BpuMI CCSGG 1 cut(s) 1731
BsaI GGTCTC 2 cut(s) 11, 477
BsaJI CCNNGG 4 cut(s) 217, 467, 1150, 1405
BsaXI ACNNNNNCTCC 2 cut(s) 1192, 1222
Bsc4I CCNNNNNNNGG 3 cut(s) 539, 1248, 1411
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 6 cut(s) 310, 657, 839, 990, 1695, 1768
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 4 cut(s) 402, 849, 1151, 1406
BseDI CCNNGG 4 cut(s) 217, 467, 1150, 1405
BseGI GGATG 5 cut(s) 194, 229, 805, 1174, 1368
BseLI CCNNNNNNNGG 3 cut(s) 539, 1248, 1411
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 4 cut(s) 647, 727, 1764, 1766
BseNI ACTGG 6 cut(s) 310, 657, 839, 990, 1695, 1768
BseRI GAGGAG 2 cut(s) 869, 967
BseSI GKGCMC 2 cut(s) 109, 956
Bsh1236I CGCG 1 cut(s) 1660
BshFI GGCC 2 cut(s) 973, 1656
BsiHKAI GWGCWC 2 cut(s) 109, 956
BsiHKCI CYCGRG 3 cut(s) 262, 1031, 1669
BsiSI CCGG 2 cut(s) 296, 1730
BslFI GGGAC 3 cut(s) 161, 415, 1167
BslI CCNNNNNNNGG 3 cut(s) 539, 1248, 1411
BsmAI GTCTC 3 cut(s) 11, 477, 1055
BsmFI GGGAC 3 cut(s) 161, 415, 1167
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 973, 1656
Bso31I GGTCTC 2 cut(s) 11, 477
BsoBI CYCGRG 3 cut(s) 262, 1031, 1669
Bsp1286I GDGCHC 2 cut(s) 109, 956
Bsp143I GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 3 cut(s) 479, 1360, 1658
BspANI GGCC 2 cut(s) 973, 1656
BspCNI CTCAG 4 cut(s) 646, 726, 1765, 1765
BspFNI CGCG 1 cut(s) 1660
BspLI GGNNCC 4 cut(s) 624, 948, 1148, 1605
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 4 cut(s) 1165, 1233, 1598, 1611
BspTNI GGTCTC 2 cut(s) 11, 477
BsrBI CCGCTC 1 cut(s) 1360
BsrDI GCAATG 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 6 cut(s) 310, 657, 839, 990, 1695, 1768
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 4 cut(s) 217, 467, 1150, 1405
BssMI GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
BssNAI GTATAC 1 cut(s) 719
BssSI CACGAG 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 217
Bst1107I GTATAC 1 cut(s) 719
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 4 cut(s) 402, 849, 1151, 1406
Bst4CI ACNGT 3 cut(s) 725, 958, 1446
Bst6I CTCTTC 2 cut(s) 917, 1573
BstC8I GCNNGC 4 cut(s) 122, 483, 1044, 1658
BstDEI CTNAG 4 cut(s) 633, 713, 1752, 1773
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 5 cut(s) 194, 229, 805, 1174, 1368
BstFNI CGCG 1 cut(s) 1660
BstKTI GATC 8 cut(s) 374, 664, 1030, 1173, 1241, 1331, 1606, 1664
BstMAI GTCTC 3 cut(s) 11, 477, 1055
BstMBI GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
BstMWI GCNNNNNNNGC 3 cut(s) 90, 629, 1357
BstNI CCWGG 4 cut(s) 402, 849, 1151, 1406
BstNSI RCATGY 4 cut(s) 48, 124, 524, 863
BstSCI CCNGG 5 cut(s) 400, 847, 1149, 1404, 1729
BstSFI CTRYAG 4 cut(s) 417, 1122, 1186, 1442
BstSLI GKGCMC 2 cut(s) 109, 956
BstUI CGCG 1 cut(s) 1660
BstV2I GAAGAC 2 cut(s) 1064, 1785
BstX2I RGATCY 3 cut(s) 1027, 1170, 1603
BstYI RGATCY 3 cut(s) 1027, 1170, 1603
BstZ17I GTATAC 1 cut(s) 719
BsuRI GGCC 2 cut(s) 973, 1656
BtgI CCRYGG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 1724
BtsCI GGATG 5 cut(s) 194, 229, 805, 1174, 1368
BtsIMutI CAGTG 3 cut(s) 664, 1644, 1702
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 4 cut(s) 122, 483, 1044, 1658
CaiI CAGNNNCTG 1 cut(s) 1016
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 972, 1655
CsiI ACCWGGT 1 cut(s) 400
Csp6I GTAC 2 cut(s) 319, 1491
CviQI GTAC 2 cut(s) 319, 1491
DdeI CTNAG 4 cut(s) 633, 713, 1752, 1773
DpnI GATC 8 cut(s) 373, 663, 1029, 1172, 1240, 1330, 1605, 1663
DpnII GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
Eam1104I CTCTTC 2 cut(s) 917, 1573
EarI CTCTTC 2 cut(s) 917, 1573
Eco130I CCWWGG 1 cut(s) 217
Eco31I GGTCTC 2 cut(s) 11, 477
Eco57I CTGAAG 3 cut(s) 498, 738, 1038
Eco88I CYCGRG 3 cut(s) 262, 1031, 1669
EcoO109I RGGNCCY 1 cut(s) 972
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 4 cut(s) 400, 847, 1149, 1404
EcoT14I CCWWGG 1 cut(s) 217
EcoT22I ATGCAT 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 217
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 427, 459
FaqI GGGAC 3 cut(s) 161, 415, 1167
FauI CCCGC 1 cut(s) 1665
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 718
FokI GGATG 5 cut(s) 181, 216, 812, 1161, 1375
FspBI CTAG 6 cut(s) 282, 501, 534, 806, 1047, 1617
GsuI CTGGAG 2 cut(s) 870, 1678
HaeIII GGCC 2 cut(s) 973, 1656
HapII CCGG 2 cut(s) 296, 1730
HincII GTYRAC 2 cut(s) 1461, 1525
HindII GTYRAC 2 cut(s) 1461, 1525
HindIII AAGCTT 3 cut(s) 141, 1040, 1465
HinfI GANTC 5 cut(s) 605, 736, 1376, 1479, 1594
HpaII CCGG 2 cut(s) 296, 1730
HphI GGTGA 3 cut(s) 656, 972, 1697
Hpy166II GTNNAC 7 cut(s) 107, 719, 954, 1461, 1525, 1540, 1727
Hpy188I TCNGA 9 cut(s) 328, 636, 729, 918, 1142, 1435, 1533, 1599, 1755
Hpy8I GTNNAC 7 cut(s) 107, 719, 954, 1461, 1525, 1540, 1727
Hpy99I CGWCG 2 cut(s) 65, 1809
HpyAV CCTTC 7 cut(s) 279, 323, 595, 1292, 1553, 1673, 1702
HpyCH4III ACNGT 3 cut(s) 725, 958, 1446
HpyCH4IV ACGT 1 cut(s) 1723
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 629, 1357
HpyF3I CTNAG 4 cut(s) 633, 713, 1752, 1773
HpySE526I ACGT 1 cut(s) 1723
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
LmnI GCTCC 5 cut(s) 628, 772, 850, 856, 946
LweI GCATC 5 cut(s) 203, 238, 573, 1003, 1690
MabI ACCWGGT 1 cut(s) 400
MaeI CTAG 6 cut(s) 282, 501, 534, 806, 1047, 1617
MaeII ACGT 1 cut(s) 1723
MaeIII GTNAC 6 cut(s) 56, 239, 907, 928, 1087, 1612
MalI GATC 8 cut(s) 373, 663, 1029, 1172, 1240, 1330, 1605, 1663
MbiI CCGCTC 1 cut(s) 1360
MboI GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
MboII GAAGA 9 cut(s) 176, 931, 934, 1031, 1064, 1385, 1590, 1724, 1785
MfeI CAATTG 2 cut(s) 822, 936
MflI RGATCY 3 cut(s) 1027, 1170, 1603
MhlI GDGCHC 2 cut(s) 109, 956
MmeI TCCRAC 3 cut(s) 280, 707, 1273
Mph1103I ATGCAT 1 cut(s) 231
MseI TTAA 3 cut(s) 315, 690, 890
MslI CAYNNNNRTG 2 cut(s) 1005, 1011
MspI CCGG 2 cut(s) 296, 1730
MspR9I CCNGG 5 cut(s) 402, 849, 1151, 1406, 1731
MunI CAATTG 2 cut(s) 822, 936
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 4 cut(s) 402, 849, 1151, 1406
MvnI CGCG 1 cut(s) 1660
MwoI GCNNNNNNNGC 3 cut(s) 90, 629, 1357
NciI CCSGG 1 cut(s) 1731
NcoI CCATGG 1 cut(s) 217
NdeII GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
NlaIV GGNNCC 4 cut(s) 624, 948, 1148, 1605
NmeAIII GCCGAG 1 cut(s) 1327
NmuCI GTSAC 1 cut(s) 1087
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 4 cut(s) 48, 124, 524, 863
PaeI GCATGC 1 cut(s) 124
PaeR7I CTCGAG 2 cut(s) 1031, 1669
PciI ACATGT 2 cut(s) 44, 520
PcsI WCGNNNNNNNCGW 1 cut(s) 1407
PctI GAATGC 1 cut(s) 95
PfeI GAWTC 5 cut(s) 605, 736, 1376, 1479, 1594
PflMI CCANNNNNTGG 1 cut(s) 1411
PfoI TCCNGGA 1 cut(s) 847
PscI ACATGT 2 cut(s) 44, 520
Psp6I CCWGG 4 cut(s) 400, 847, 1149, 1404
PspGI CCWGG 4 cut(s) 400, 847, 1149, 1404
PspN4I GGNNCC 4 cut(s) 624, 948, 1148, 1605
PspPI GGNCC 2 cut(s) 972, 1655
PspXI VCTCGAGB 1 cut(s) 1669
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 3 cut(s) 1027, 1170, 1603
RsaI GTAC 2 cut(s) 320, 1492
RsaNI GTAC 2 cut(s) 319, 1491
RseI CAYNNNNRTG 2 cut(s) 1005, 1011
SaqAI TTAA 3 cut(s) 315, 690, 890
Sau3AI GATC 8 cut(s) 371, 661, 1027, 1170, 1238, 1328, 1603, 1661
Sau96I GGNCC 2 cut(s) 972, 1655
ScrFI CCNGG 5 cut(s) 402, 849, 1151, 1406, 1731
SduI GDGCHC 2 cut(s) 109, 956
SexAI ACCWGGT 1 cut(s) 400
SfaNI GCATC 5 cut(s) 203, 238, 573, 1003, 1690
SfcI CTRYAG 4 cut(s) 417, 1122, 1186, 1442
Sfr274I CTCGAG 2 cut(s) 1031, 1669
SlaI CTCGAG 2 cut(s) 1031, 1669
SmiMI CAYNNNNRTG 2 cut(s) 1005, 1011
SmlI CTYRAG 4 cut(s) 1031, 1066, 1217, 1669
SmoI CTYRAG 4 cut(s) 1031, 1066, 1217, 1669
SphI GCATGC 1 cut(s) 124
SsiI CCGC 3 cut(s) 479, 1360, 1658
SspI AATATT 3 cut(s) 597, 1321, 1425
SspMI CTAG 6 cut(s) 282, 501, 534, 806, 1047, 1617
StyD4I CCNGG 5 cut(s) 400, 847, 1149, 1404, 1729
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 3 cut(s) 725, 958, 1446
TaiI ACGT 1 cut(s) 1726
TaqI TCGA 6 cut(s) 1032, 1237, 1379, 1551, 1670, 1804
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 5 cut(s) 605, 736, 1376, 1479, 1594
Tru1I TTAA 3 cut(s) 315, 690, 890
Tru9I TTAA 3 cut(s) 315, 690, 890
TscAI CASTG 3 cut(s) 664, 1644, 1702
TseFI GTSAC 1 cut(s) 1087
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 7 cut(s) 266, 1096, 1126, 1146, 1475, 1692, 1785
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 3 cut(s) 664, 1644, 1702
Van91I CCANNNNNTGG 1 cut(s) 1411
VneI GTGCAC 2 cut(s) 105, 952
XapI RAATTY 5 cut(s) 412, 1133, 1225, 1296, 1389
XbaI TCTAGA 1 cut(s) 281
XceI RCATGY 4 cut(s) 48, 124, 524, 863
XhoI CTCGAG 2 cut(s) 1031, 1669
XmiI GTMKAC 1 cut(s) 718
XspI CTAG 6 cut(s) 282, 501, 534, 806, 1047, 1617
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.