MD14G1211300.v1.1

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001632
Physical Location & Seq
Forward (+)
29674111 .. 29674681
571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1211300.v1.1.491

Sequence Viewer

Length: 480 bp
ATGCATCCCAAATGCTTGATTGATATTTCTTTCTTTAGGGGAGGGTTCATTGTTGATGCAGCTACAACTAGTGAATTCATCGGAGGGTTCCAGGGACGGCCTCCTTGTAAGATCCATCCTAAAGCCTATAGATTTGCACAAACAATGCCTCGTGTCCTCAAGGCGAATTTTCTTCCTCGATCCCAACTTTGGACTGATATCTTCCAAGACCAACCTCCTGACCTTCAAGATGTTGGACTTTACTTTTTCCCTGATGAAAACGCTGAGAGATCAAGAGAGGGCCATGCTCGGCTGATTGAGCGGATGGAGACCGAAGATTCAATGATGAGAATTTGCTTTGATGACGAGGGCGTTGAGTTGCTGATATTTACATCAGAAAAGCTACAGTTAGACAAGCAGTGTGAGTTTTTTCCTTATGAACTTCCTTTTCTCTTGCAGCGACTTCAGATTTCCTTCGTCTGGCAAGGCCATTTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

160

Amino Acids

18.64

Weight (kDa)

5.09

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC_AIPP2 PF23121 13 - 134 7.2e-33 AIPP2, SPOC-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 301
AciI CCGC 1 cut(s) 301
AclWI GGATC 2 cut(s) 106, 174
AcsI RAATTY 3 cut(s) 74, 166, 330
AcuI CTGAAG 1 cut(s) 428
AfiI CCNNNNNNNGG 2 cut(s) 189, 459
AgsI TTSAA 2 cut(s) 227, 321
AhlI ACTAGT 1 cut(s) 68
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 2 cut(s) 62, 382
AluI AGCT 2 cut(s) 62, 382
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 2 cut(s) 106, 174
AoxI GGCC 3 cut(s) 98, 280, 466
ApeKI GCWGC 2 cut(s) 59, 436
ApoI RAATTY 3 cut(s) 74, 166, 330
AspS9I GGNCC 1 cut(s) 280
BauI CACGAG 1 cut(s) 150
BbvI GCAGC 2 cut(s) 71, 448
BccI CCATC 2 cut(s) 123, 298
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 302
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 127, 383
BisI GCNGC 2 cut(s) 60, 437
BlsI GCNGC 2 cut(s) 61, 438
Bme1390I CCNGG 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 2 cut(s) 13, 46
BpuEI CTTGAG 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 459
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 3 cut(s) 4, 115, 309
BseLI CCNNNNNNNGG 2 cut(s) 189, 459
BseMII CTCAG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 71, 448
BshFI GGCC 3 cut(s) 100, 282, 468
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 2 cut(s) 189, 459
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 108
BsnI GGCC 3 cut(s) 100, 282, 468
Bso31I GGTCTC 1 cut(s) 302
Bsp143I GATC 3 cut(s) 111, 179, 269
BspACI CCGC 1 cut(s) 301
BspANI GGCC 3 cut(s) 100, 282, 468
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 89
BspPI GGATC 2 cut(s) 106, 174
BspTNI GGTCTC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 3 cut(s) 111, 179, 269
BssSI CACGAG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 4, 115, 309
BstKTI GATC 3 cut(s) 114, 182, 272
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 3 cut(s) 111, 179, 269
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 2 cut(s) 127, 383
BstV1I GCAGC 2 cut(s) 71, 448
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BsuRI GGCC 3 cut(s) 100, 282, 468
BtsCI GGATG 3 cut(s) 4, 115, 309
BtsI GCAGTG 1 cut(s) 404
BtsIMutI CAGTG 1 cut(s) 404
Cfr13I GGNCC 1 cut(s) 280
CviAII CATG 1 cut(s) 284
CviJI RGCY 7 cut(s) 62, 100, 125, 282, 292, 382, 468
CviKI_1 RGCY 7 cut(s) 62, 100, 125, 282, 292, 382, 468
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 113, 181, 271
DpnII GATC 3 cut(s) 111, 179, 269
Eco31I GGTCTC 1 cut(s) 302
Eco32I GATATC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 90
EcoRV GATATC 1 cut(s) 199
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 287
FaiI YATR 3 cut(s) 129, 285, 417
FaqI GGGAC 1 cut(s) 108
FatI CATG 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 60, 437
FokI GGATG 2 cut(s) 102, 316
Fsp4HI GCNGC 2 cut(s) 60, 437
FspBI CTAG 1 cut(s) 69
GluI GCNGC 2 cut(s) 60, 437
HaeIII GGCC 3 cut(s) 100, 282, 468
Hin1II CATG 1 cut(s) 287
HinfI GANTC 1 cut(s) 317
Hpy188I TCNGA 3 cut(s) 83, 376, 447
Hpy188III TCNNGA 3 cut(s) 218, 227, 273
HpyAV CCTTC 2 cut(s) 233, 463
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4V TGCA 4 cut(s) 4, 59, 137, 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 1 cut(s) 287
Kzo9I GATC 3 cut(s) 111, 179, 269
LpnPI CCDG 5 cut(s) 77, 104, 231, 264, 445
Lsp1109I GCAGC 2 cut(s) 71, 448
LweI GCATC 2 cut(s) 13, 46
MaeI CTAG 1 cut(s) 69
MalI GATC 3 cut(s) 113, 181, 271
MbiI CCGCTC 1 cut(s) 301
MboI GATC 3 cut(s) 111, 179, 269
MboII GAAGA 3 cut(s) 164, 193, 326
MflI RGATCY 1 cut(s) 111
MluCI AATT 3 cut(s) 74, 166, 330
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 9 cut(s) 35, 77, 111, 159, 167, 186, 225, 271, 340
Mph1103I ATGCAT 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 298
NdeII GATC 3 cut(s) 111, 179, 269
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 89
NmeAIII GCCGAG 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 2 cut(s) 61, 438
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 280
PsuI RGATCY 1 cut(s) 111
SatI GCNGC 2 cut(s) 60, 437
Sau3AI GATC 3 cut(s) 111, 179, 269
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 92
SetI ASST 4 cut(s) 64, 217, 225, 384
SfaNI GCATC 2 cut(s) 13, 46
SfcI CTRYAG 2 cut(s) 127, 383
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 68
Sse9I AATT 3 cut(s) 74, 166, 330
SsiI CCGC 1 cut(s) 301
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 387
TaqI TCGA 1 cut(s) 178
TaqII GACCGA 1 cut(s) 326
TasI AATT 3 cut(s) 74, 166, 330
TfiI GAWTC 1 cut(s) 317
TscAI CASTG 1 cut(s) 404
TseI GCWGC 2 cut(s) 59, 436
TspDTI ATGAA 4 cut(s) 37, 67, 270, 432
TspRI CASTG 1 cut(s) 404
XapI RAATTY 3 cut(s) 74, 166, 330
XspI CTAG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.