MD06G1222600.v1.1

Glycosyltransferase-like domain-containing protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001096
Physical Location & Seq
Reverse (-)
35402904 .. 35404417
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222600.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGATATGTTTGGTGTGTAACTCTCGTTTACTCGCAGTGAAATTGCAGATGTCAGTGGGTCTTGGTTGGGACGTGCTGCAAGTCCAACAAACAAACAGCTCTCAGCAAGTAATCGAAAGTGAGCAGCTTAATCAGAAGATAAGAGCGGAGGAATTGAAAAATGCAAGTCTTCAAGGAGAGGATTCTGGTGTACAGAAAAGTGCAAGCAATCCCACTATTCTCATTGCTCGAAATGGTTCAGAATCAGAGACAAACATTTCAGAAGTGACGAAGAATTTGGAGCCAATTTGTGATGTTTTGCAGCCAAGAACTGATGTTTGTGAGATCAGCATGGATGTTCATGTTGATGGTAATTTGTCTTCGGTTTTCGTTGTTTCATCTCAAGCGGACATGCTGGCAGGGAATAGCTCCTGGACGATAAGGCCGTATGCTCGAAAAGAAGATAAACAGGCGCTGAATCATGTTCGAGAGTGGTCAGTGAAACGAGTGTCTGATGCTCAGCAGGAAATCCTTCAATGCACCAGAAATCATAGTGTTCCAGCCGTCTTATTTTCAACCGGAGGATATACAGGAAACCATTTCCATGAATTCACAGACATGGTCATTCCGCTGTATATTACTTCTCGAAAATATGATGGGGAAGTTCAGTTTCTGATTACGGATAAAAGAAGCAGGTGGATCGAAAAGTACCAATCAATACTAAAAGGGCTGTCCAAGTATGAGATTATTGACATTGACAAGGAAGATGCAGTGCATTGCTTCCCAAGCGTAACGGTTGGACTTAAAAGACATCCAAAGGATCTGAGCATTGATCCTTCGAAACATTCATATTCGATTACTGACTTCCGAGCATTCTTAAGAAAAACTTACTCACTAAAGAAGGCCAATGCAATCAGAATCAGAGGTGGTGGCCGGCATAAAATGCCCCGGCTTCTCATCATATCGAGAAGGAGCACAAGGTGTTTCATGAATCCGAGACAAATTACTAAGATGGCTAGAAGCTTGGGATACAAGGTAACTGTTATGGAGGCTGACAGGAACATGTCAAGAACTGCAGAAGTTGTGAACTCTTGTGATGTGTTAATGGGGGTTCACGGAGCTGCCCTCACAAACATTCTGTTCCTCCCTGAAAATGCAGTTCTTATCCAAATACTTCCGCTCGGAGGGTTCGAATGGCTTGCAACAAACTACTTCGGAGAGCCATCGAGGGATATGAAGATCAAGTATTTGGAATACAAGATCAGCAGAAGCGAGAGCACGCTGAAAATCGACCCGAATAAGAATCGAATACGAGGATGGCAAGCATTCAAGTCAACTTTTCTGAATGCACAAAATGTAACCCTTAACGTCAAAAGGATTAGAACCACTTTGAAAAAAGCCCTTGGACTTCTGCATCAGATTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

469

Amino Acids

53.02

Weight (kDa)

9.47

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 276 - 382 4.8e-13 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 663
AccBSI CCGCTC 2 cut(s) 146, 1159
AciI CCGC 4 cut(s) 146, 386, 608, 1157
AclWI GGATC 3 cut(s) 686, 806, 807
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 2 cut(s) 274, 587
AdeI CACNNNGTG 1 cut(s) 960
AfaI GTAC 2 cut(s) 192, 689
AfiI CCNNNNNNNGG 1 cut(s) 1163
AflII CTTAAG 1 cut(s) 856
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 6 cut(s) 157, 173, 515, 555, 1309, 1372
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 1 cut(s) 410
AluBI AGCT 5 cut(s) 99, 127, 408, 1002, 1100
AluI AGCT 5 cut(s) 99, 127, 408, 1002, 1100
Alw21I GWGCWC 2 cut(s) 956, 1259
Alw26I GTCTC 2 cut(s) 242, 970
AlwI GGATC 3 cut(s) 686, 806, 807
AlwNI CAGNNNCTG 2 cut(s) 454, 652
AoxI GGCC 3 cut(s) 422, 882, 910
ApeKI GCWGC 4 cut(s) 76, 124, 301, 1100
ApoI RAATTY 2 cut(s) 274, 587
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
Asp700I GAANNNNTTC 2 cut(s) 235, 510
AspLEI GCGC 1 cut(s) 454
AsuC2I CCSGG 1 cut(s) 928
AsuII TTCGAA 2 cut(s) 818, 1170
BarI GAAGNNNNNNTAC 2 cut(s) 343, 375
BbsI GAAGAC 2 cut(s) 161, 351
Bbv12I GWGCWC 2 cut(s) 956, 1259
BbvI GCAGC 4 cut(s) 63, 136, 313, 1087
BccI CCATC 5 cut(s) 341, 629, 985, 1210, 1290
BceAI ACGGC 2 cut(s) 409, 527
BcgI CGANNNNNNTGC 4 cut(s) 661, 695, 1160, 1194
BciT130I CCWGG 1 cut(s) 412
BciVI GTATCC 1 cut(s) 1001
BcnI CCSGG 1 cut(s) 928
BcoDI GTCTC 2 cut(s) 242, 970
BfaI CTAG 1 cut(s) 996
BfmI CTRYAG 1 cut(s) 1053
BfoI RGCGCY 1 cut(s) 455
BfrI CTTAAG 1 cut(s) 856
BfuAI ACCTGC 1 cut(s) 663
BfuI GTATCC 1 cut(s) 1001
BisI GCNGC 4 cut(s) 77, 125, 302, 1101
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 4 cut(s) 78, 126, 303, 1102
Bme1390I CCNGG 2 cut(s) 412, 928
BmgBI CACGTC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 2 cut(s) 412, 928
BmsI GCATC 3 cut(s) 484, 736, 1402
BpiI GAAGAC 2 cut(s) 161, 351
BplI GAGNNNNNCTC 2 cut(s) 1089, 1121
Bpu1102I GCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 818, 1170
BpuEI CTTGAG 1 cut(s) 366
BpuMI CCSGG 1 cut(s) 928
BsaJI CCNNGG 2 cut(s) 926, 1381
BsaWI WCCGGW 1 cut(s) 557
Bsc4I CCNNNNNNNGG 1 cut(s) 1163
Bse118I RCCGGY 1 cut(s) 912
Bse3DI GCAATG 2 cut(s) 222, 754
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 2 cut(s) 926, 1381
BseGI GGATG 3 cut(s) 340, 790, 1301
BseLI CCNNNNNNNGG 1 cut(s) 1163
BseMI GCAATG 2 cut(s) 222, 754
BseMII CTCAG 3 cut(s) 116, 512, 794
BseXI GCAGC 4 cut(s) 63, 136, 313, 1087
BshFI GGCC 3 cut(s) 424, 884, 912
BsiHKAI GWGCWC 2 cut(s) 956, 1259
BsiSI CCGG 3 cut(s) 558, 913, 928
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 1163
BsmAI GTCTC 2 cut(s) 242, 970
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 3 cut(s) 851, 1304, 1330
BsnI GGCC 3 cut(s) 424, 884, 912
Bsp119I TTCGAA 2 cut(s) 818, 1170
Bsp1286I GDGCHC 2 cut(s) 956, 1259
Bsp1407I TGTACA 1 cut(s) 190
Bsp143I GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
Bsp1720I GCTNAGC 1 cut(s) 498
BspACI CCGC 4 cut(s) 146, 386, 608, 1157
BspANI GGCC 3 cut(s) 424, 884, 912
BspCNI CTCAG 3 cut(s) 115, 511, 795
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 1 cut(s) 282
BspMAI CTGCAG 1 cut(s) 1057
BspMI ACCTGC 1 cut(s) 663
BspPI GGATC 3 cut(s) 686, 806, 807
BspT104I TTCGAA 2 cut(s) 818, 1170
BspTI CTTAAG 1 cut(s) 856
BsrBI CCGCTC 2 cut(s) 146, 1159
BsrDI GCAATG 2 cut(s) 222, 754
BsrFI RCCGGY 1 cut(s) 912
BsrGI TGTACA 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 912
BssECI CCNNGG 2 cut(s) 926, 1381
BssMI GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
BssT1I CCWWGG 1 cut(s) 1381
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 775, 1021
BstAFI CTTAAG 1 cut(s) 856
BstAPI GCANNNNNTGC 1 cut(s) 922
BstAUI TGTACA 1 cut(s) 190
BstBI TTCGAA 2 cut(s) 818, 1170
BstC8I GCNNGC 6 cut(s) 205, 396, 914, 1179, 1259, 1302
BstDEI CTNAG 4 cut(s) 102, 498, 803, 987
BstF5I GGATG 3 cut(s) 340, 790, 1301
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 6 cut(s) 327, 681, 802, 814, 1221, 1242
BstMAI GTCTC 2 cut(s) 242, 970
BstMBI GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
BstMWI GCNNNNNNNGC 2 cut(s) 765, 922
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 2 cut(s) 394, 1045
BstSCI CCNGG 2 cut(s) 410, 926
BstSFI CTRYAG 1 cut(s) 1053
BstV1I GCAGC 4 cut(s) 63, 136, 313, 1087
BstV2I GAAGAC 2 cut(s) 161, 351
BstX2I RGATCY 1 cut(s) 799
BstYI RGATCY 1 cut(s) 799
BsuI GTATCC 1 cut(s) 1001
BsuRI GGCC 3 cut(s) 424, 884, 912
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 3 cut(s) 340, 790, 1301
BtsI GCAGTG 2 cut(s) 42, 756
BtsIMutI CAGTG 4 cut(s) 42, 60, 483, 756
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 6 cut(s) 205, 396, 914, 1179, 1259, 1302
CaiI CAGNNNCTG 2 cut(s) 454, 652
CciI TCATGA 1 cut(s) 966
CfoI GCGC 1 cut(s) 454
Cfr10I RCCGGY 1 cut(s) 912
Csp6I GTAC 2 cut(s) 191, 688
CviAII CATG 8 cut(s) 331, 341, 391, 461, 584, 598, 967, 1042
CviQI GTAC 2 cut(s) 191, 688
DdeI CTNAG 4 cut(s) 102, 498, 803, 987
DpnI GATC 6 cut(s) 326, 680, 801, 813, 1220, 1241
DpnII GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
DraIII CACNNNGTG 1 cut(s) 960
EaeI YGGCCR 1 cut(s) 910
Eco130I CCWWGG 1 cut(s) 1381
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 1381
ErhI CCWWGG 1 cut(s) 1381
FaeI CATG 8 cut(s) 334, 344, 394, 464, 587, 601, 970, 1045
FalI AAGNNNNNCTT 2 cut(s) 850, 882
FaqI GGGAC 1 cut(s) 83
FatI CATG 8 cut(s) 330, 340, 390, 460, 583, 597, 966, 1041
Fnu4HI GCNGC 4 cut(s) 77, 125, 302, 1101
FokI GGATG 3 cut(s) 347, 777, 1308
Fsp4HI GCNGC 4 cut(s) 77, 125, 302, 1101
FspBI CTAG 1 cut(s) 996
GlaI GCGC 1 cut(s) 453
GluI GCNGC 4 cut(s) 77, 125, 302, 1101
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 3 cut(s) 424, 884, 912
HapII CCGG 3 cut(s) 558, 913, 928
HhaI GCGC 1 cut(s) 454
Hin1II CATG 8 cut(s) 334, 344, 394, 464, 587, 601, 970, 1045
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HincII GTYRAC 1 cut(s) 1314
HindII GTYRAC 1 cut(s) 1314
HindIII AAGCTT 1 cut(s) 1000
HinfI GANTC 7 cut(s) 182, 242, 457, 897, 970, 1282, 1399
HpaII CCGG 3 cut(s) 558, 913, 928
Hpy166II GTNNAC 5 cut(s) 29, 191, 1066, 1093, 1314
Hpy188III TCNNGA 5 cut(s) 467, 624, 945, 967, 1047
Hpy8I GTNNAC 5 cut(s) 29, 191, 1066, 1093, 1314
HpyAV CCTTC 4 cut(s) 521, 825, 874, 942
HpyCH4III ACNGT 2 cut(s) 775, 1021
HpyCH4IV ACGT 2 cut(s) 72, 1347
HpyF10VI GCNNNNNNNGC 2 cut(s) 765, 922
HpyF3I CTNAG 4 cut(s) 102, 498, 803, 987
HpySE526I ACGT 2 cut(s) 72, 1347
Hsp92II CATG 8 cut(s) 334, 344, 394, 464, 587, 601, 970, 1045
HspAI GCGC 1 cut(s) 452
KroI GCCGGC 1 cut(s) 912
KroNI GCCGGC 1 cut(s) 914
Kzo9I GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
LmnI GCTCC 4 cut(s) 280, 413, 951, 1097
Lsp1109I GCAGC 4 cut(s) 63, 136, 313, 1087
LweI GCATC 3 cut(s) 484, 736, 1402
MaeI CTAG 1 cut(s) 996
MaeII ACGT 2 cut(s) 72, 1347
MaeIII GTNAC 5 cut(s) 17, 265, 769, 1015, 1336
MalI GATC 6 cut(s) 326, 680, 801, 813, 1220, 1241
MbiI CCGCTC 2 cut(s) 146, 1159
MboI GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
MboII GAAGA 7 cut(s) 148, 161, 283, 351, 452, 755, 1228
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 2 cut(s) 956, 1259
MluCI AATT 7 cut(s) 41, 152, 274, 285, 352, 587, 981
MmeI TCCRAC 2 cut(s) 109, 757
MroNI GCCGGC 1 cut(s) 912
MroXI GAANNNNTTC 2 cut(s) 235, 510
MseI TTAA 5 cut(s) 129, 783, 857, 1082, 1344
MslI CAYNNNNRTG 3 cut(s) 345, 582, 596
MspA1I CMGCKG 1 cut(s) 610
MspCI CTTAAG 1 cut(s) 856
MspI CCGG 3 cut(s) 558, 913, 928
MspR9I CCNGG 2 cut(s) 412, 928
Mva1269I GAATGC 3 cut(s) 851, 1304, 1330
MvaI CCWGG 1 cut(s) 412
MwoI GCNNNNNNNGC 2 cut(s) 765, 922
NaeI GCCGGC 1 cut(s) 914
NciI CCSGG 1 cut(s) 928
NdeII GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
NgoMIV GCCGGC 1 cut(s) 912
NlaIII CATG 8 cut(s) 334, 344, 394, 464, 587, 601, 970, 1045
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 2 cut(s) 394, 1045
NspV TTCGAA 2 cut(s) 818, 1170
PagI TCATGA 1 cut(s) 966
PaqCI CACCTGC 1 cut(s) 663
PciI ACATGT 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 2 cut(s) 422, 1167
PctI GAATGC 3 cut(s) 851, 1304, 1330
PdiI GCCGGC 1 cut(s) 914
PdmI GAANNNNTTC 2 cut(s) 235, 510
PfeI GAWTC 7 cut(s) 182, 242, 457, 897, 970, 1282, 1399
PflFI GACNNNGTC 1 cut(s) 599
PfoI TCCNGGA 1 cut(s) 410
PkrI GCNGC 4 cut(s) 78, 126, 303, 1102
PscI ACATGT 1 cut(s) 1041
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 1 cut(s) 282
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 2 cut(s) 454, 652
PsuI RGATCY 1 cut(s) 799
PsyI GACNNNGTC 1 cut(s) 599
RsaI GTAC 2 cut(s) 192, 689
RsaNI GTAC 2 cut(s) 191, 688
RseI CAYNNNNRTG 3 cut(s) 345, 582, 596
SaqAI TTAA 5 cut(s) 129, 783, 857, 1082, 1344
SatI GCNGC 4 cut(s) 77, 125, 302, 1101
Sau3AI GATC 6 cut(s) 324, 678, 799, 811, 1218, 1239
ScrFI CCNGG 2 cut(s) 412, 928
SduI GDGCHC 2 cut(s) 956, 1259
SfaNI GCATC 3 cut(s) 484, 736, 1402
SfcI CTRYAG 1 cut(s) 1053
SfuI TTCGAA 2 cut(s) 818, 1170
SmiMI CAYNNNNRTG 3 cut(s) 345, 582, 596
SmlI CTYRAG 2 cut(s) 381, 856
SmoI CTYRAG 2 cut(s) 381, 856
Sse9I AATT 7 cut(s) 41, 152, 274, 285, 352, 587, 981
SsiI CCGC 4 cut(s) 146, 386, 608, 1157
SspMI CTAG 1 cut(s) 996
StyD4I CCNGG 2 cut(s) 410, 926
StyI CCWWGG 1 cut(s) 1381
TaaI ACNGT 2 cut(s) 775, 1021
TaiI ACGT 2 cut(s) 75, 1350
TasI AATT 7 cut(s) 41, 152, 274, 285, 352, 587, 981
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 7 cut(s) 182, 242, 457, 897, 970, 1282, 1399
Tru1I TTAA 5 cut(s) 129, 783, 857, 1082, 1344
Tru9I TTAA 5 cut(s) 129, 783, 857, 1082, 1344
TscAI CASTG 4 cut(s) 42, 60, 483, 756
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 4 cut(s) 76, 124, 301, 1100
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 7 cut(s) 329, 366, 600, 816, 955, 983, 1229
TspGWI ACGGA 2 cut(s) 674, 1110
TspRI CASTG 4 cut(s) 42, 60, 483, 756
Tth111I GACNNNGTC 1 cut(s) 599
Vha464I CTTAAG 1 cut(s) 856
XapI RAATTY 2 cut(s) 274, 587
XceI RCATGY 2 cut(s) 394, 1045
XmnI GAANNNNTTC 2 cut(s) 235, 510
XspI CTAG 1 cut(s) 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.