MD06G1222700.v1.1

Glycosyltransferase-like domain-containing protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001096
Physical Location & Seq
Reverse (-)
35406697 .. 35408476
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222700.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGCGGTGATGTATGATTCGATACTAGCCCGAAGCTTTAGTAAGCACGAGCAGAAGAAATTGAGATATGGAGCATTCGTTTGCTGCTTGCTCATAGCACTGTGCTTCTGCACTCTGCTGAAACCTAATTTGAATCCTCTACCAGCTTTGAAGTTGGAGAGATCAGTTGGTGTCAAACAAAAGATATTGGCACTCTGGGAAACAAACAGCACTCAGCAAGTGATAAAAAATGTGCTAGAATTTCCGGAGATAAAAACCGAGGAGTTTGAAAATTTGAGTGATCAAGTGTTAGCAGATTCTGCTGATTTGCAGCAAAATGCAAGCATTCTCACTAGTCCCATTGAAAGCAGCATGTCGGTAGGTGAGGAGACTAAGAGTAAGCACCCTTCCTCCAATACAGCTGGTACAAACATAAAGAAACCATTAACTAACGCGAACATTTTTGCAGTTGTGGAAGTTGTAGAGGAGAATTTGGAGCCACTTTGCAAAACTGAGGAACCAAGAACCGAATACTGTGAGATCATTAACAGGGATGTTCGCGTAGATGCAAACTCTTCCTCAGTTTTTGTTGCTTCATCTTCTCAAATTTGGAACAGGTCCTGGACTATAAGACCTTATGCTCGAAAAGAGGACAAAACTGCGTTGGGCAGTACTCGAGCATGGTCAGTGAAACCAGTACAAACTGGTGCTCAGCTCAATATCCCTCAGTGTACTCGAGATCATAGCGTTCCAGCCATCTTGTTTTCTACTGGAGGATACGTAGGAAACCATTTCCATGAGTTCACTGATGTGGTGATTCCACTGTTTATTACTTCTCGAAAATATGATGGAGAAGTTCAATTTCTTGTTTCAGATATGAAACCATGGTGGATTGCAAAGTACCAAGCAATACTAAAAGGACTGTCCAAGTACGAGATTATCGACATTGACAAAGAAGATGCAGTACATTGCTTCCCAAGCGTAACCGTTGGCCTTAAACGACACGAAAAGGAGCTGAGCATTGATCCTTCAAAGCATTCATATTCCATGAAAGACTTCAGAGAGTTTCTAAGAAACCGTTACTCGTTGAAAAGAGCCAGTGCAATCAGAATCAGAGAAAATCGTCAGCGAAAAAGGCCCCGGCTTCTGATCATCCCAAGAAAGAGAACAAGGTCTTTCACGAACACAGGGGAAATTACGAAAATGGCTAGACGCTTGGGGTTCAAGGTAATCGTCGCAGAAGCTGACATGAACTTGTCTAAATTTGCAGAAGTAGTCAACTCTTGTGATGTGTTAGTGGGAGTTCATGGAGCTGGCCTCACAAACCTTGTCTTCCTTCCAGAAAATGCAATTTTGATTCAAATACTTCCAGTCGGACGGTTCCAATGGCTCGCAACCAACTACTTCGGCAAGCCTTCGGAGGGTATGAATCTCAAGTATTTAGAGTACGAAATAAGCAACGAGGAGAGCACGTTGACACAGCAGTACCCGCTTGATCACCCGGTTTTCACCGATCCTTCATCGATCGGAAAACAGGGTTGGCTAGCGTTCTATTCACTCTATCTGCAAAAACAAAACGTACATCTTAATGTCCAAAGATTTAGACCTACATTGTTAAAAGCTCTTGAGCTTCTGCATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

541

Amino Acids

61.46

Weight (kDa)

9.04

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 340 - 445 6.4e-13 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 434, 540
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 2 cut(s) 5, 1469
AclWI GGATC 2 cut(s) 998, 1487
AcsI RAATTY 5 cut(s) 239, 271, 469, 585, 1241
AcuI CTGAAG 1 cut(s) 1021
AfiI CCNNNNNNNGG 1 cut(s) 1400
AgsI TTSAA 9 cut(s) 133, 151, 269, 344, 839, 1011, 1069, 1204, 1340
AhlI ACTAGT 1 cut(s) 332
AjnI CCWGG 1 cut(s) 599
AjuI GAANNNNNNNTTGG 2 cut(s) 1501, 1533
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 9 cut(s) 36, 146, 401, 694, 994, 1223, 1292, 1601, 1609
AluI AGCT 9 cut(s) 36, 146, 401, 694, 994, 1223, 1292, 1601, 1609
Alw21I GWGCWC 2 cut(s) 691, 1451
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 2 cut(s) 998, 1487
AlwNI CAGNNNCTG 3 cut(s) 299, 600, 1223
Ama87I CYCGRG 2 cut(s) 654, 714
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 3 cut(s) 970, 1115, 1294
ApeKI GCWGC 3 cut(s) 84, 310, 348
ApoI RAATTY 5 cut(s) 239, 271, 469, 585, 1241
Asp700I GAANNNNTTC 1 cut(s) 1034
AspS9I GGNCC 2 cut(s) 597, 1116
AsuC2I CCSGG 2 cut(s) 1120, 1481
AsuHPI GGTGA 5 cut(s) 19, 374, 805, 1469, 1480
AsuNHI GCTAGC 1 cut(s) 1522
AvaI CYCGRG 2 cut(s) 654, 714
AvaII GGWCC 1 cut(s) 597
BaeI ACNNNNGTAYC 2 cut(s) 1448, 1481
BarI GAAGNNNNNNTAC 4 cut(s) 370, 402, 927, 959
BauI CACGAG 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 1303
Bbv12I GWGCWC 2 cut(s) 691, 1451
BbvI GCAGC 3 cut(s) 71, 322, 360
BccI CCATC 2 cut(s) 743, 821
BciT130I CCWGG 1 cut(s) 601
BciVI GTATCC 1 cut(s) 749
BclI TGATCA 3 cut(s) 280, 1128, 1474
BcnI CCSGG 2 cut(s) 1120, 1481
BcoDI GTCTC 1 cut(s) 362
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 5 cut(s) 26, 236, 333, 1188, 1523
BfuI GTATCC 1 cut(s) 749
BisI GCNGC 3 cut(s) 85, 311, 349
BlpI GCTNAGC 2 cut(s) 690, 995
BlsI GCNGC 3 cut(s) 86, 312, 350
BmcAI AGTACT 1 cut(s) 652
Bme1390I CCNGG 3 cut(s) 601, 1120, 1481
Bme18I GGWCC 1 cut(s) 597
BmeT110I CYCGRG 2 cut(s) 654, 714
BmgT120I GGNCC 2 cut(s) 597, 1116
BmiI GGNNCC 4 cut(s) 477, 498, 1118, 1361
BmrFI CCNGG 3 cut(s) 601, 1120, 1481
BmsI GCATC 2 cut(s) 535, 928
BmtI GCTAGC 1 cut(s) 1526
BpiI GAAGAC 1 cut(s) 1303
BplI GAGNNNNNCTC 2 cut(s) 1281, 1313
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 2 cut(s) 690, 995
BpuEI CTTGAG 1 cut(s) 1397
BpuMI CCSGG 2 cut(s) 1120, 1481
Bsa29I ATCGAT 1 cut(s) 1502
BsaAI YACGTR 1 cut(s) 760
BsaJI CCNNGG 3 cut(s) 258, 863, 1118
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 8 cut(s) 63, 93, 374, 404, 744, 774, 1436, 1466
Bsc4I CCNNNNNNNGG 1 cut(s) 1400
Bse1I ACTGG 5 cut(s) 674, 688, 754, 1077, 1349
Bse3DI GCAATG 1 cut(s) 946
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 1 cut(s) 601
BseCI ATCGAT 1 cut(s) 1502
BseDI CCNNGG 3 cut(s) 258, 863, 1118
BseGI GGATG 2 cut(s) 538, 1131
BseLI CCNNNNNNNGG 1 cut(s) 1400
BseMI GCAATG 1 cut(s) 946
BseMII CTCAG 6 cut(s) 227, 483, 573, 704, 719, 986
BseNI ACTGG 5 cut(s) 674, 688, 754, 1077, 1349
BseRI GAGGAG 4 cut(s) 275, 380, 479, 1457
BseXI GCAGC 3 cut(s) 71, 322, 360
BsgI GTGCAG 1 cut(s) 94
Bsh1236I CGCG 2 cut(s) 434, 540
Bsh1285I CGRYCG 1 cut(s) 1506
BshFI GGCC 3 cut(s) 972, 1117, 1296
BshVI ATCGAT 1 cut(s) 1502
BsiEI CGRYCG 1 cut(s) 1506
BsiHKAI GWGCWC 2 cut(s) 691, 1451
BsiHKCI CYCGRG 2 cut(s) 654, 714
BsiSI CCGG 3 cut(s) 245, 1120, 1481
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 1400
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 321
BsmI GAATGC 3 cut(s) 74, 324, 1015
BsnI GGCC 3 cut(s) 972, 1117, 1296
BsoBI CYCGRG 2 cut(s) 654, 714
Bsp1286I GDGCHC 2 cut(s) 691, 1451
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
Bsp1720I GCTNAGC 2 cut(s) 690, 995
Bsp19I CCATGG 1 cut(s) 863
BspACI CCGC 2 cut(s) 5, 1469
BspANI GGCC 3 cut(s) 972, 1117, 1296
BspCNI CTCAG 6 cut(s) 226, 484, 572, 703, 718, 987
BspDI ATCGAT 1 cut(s) 1502
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 434, 540
BspLI GGNNCC 4 cut(s) 477, 498, 1118, 1361
BspOI GCTAGC 1 cut(s) 1526
BspPI GGATC 2 cut(s) 998, 1487
BsrDI GCAATG 1 cut(s) 946
BsrI ACTGG 5 cut(s) 674, 688, 754, 1077, 1349
BssECI CCNNGG 3 cut(s) 258, 863, 1118
BssMI GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
BssSI CACGAG 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 863
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 7 cut(s) 102, 515, 804, 903, 967, 1058, 1359
Bst6I CTCTTC 1 cut(s) 559
BstAPI GCANNNNNTGC 1 cut(s) 299
BstBAI YACGTR 1 cut(s) 760
BstC8I GCNNGC 6 cut(s) 89, 322, 1294, 1371, 1391, 1524
BstDEI CTNAG 8 cut(s) 213, 372, 492, 559, 690, 705, 995, 1049
BstDSI CCRYGG 1 cut(s) 863
BstF5I GGATG 2 cut(s) 538, 1131
BstFNI CGCG 2 cut(s) 434, 540
BstKTI GATC 9 cut(s) 164, 283, 522, 721, 1006, 1131, 1477, 1495, 1506
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
BstMCI CGRYCG 1 cut(s) 1506
BstMWI GCNNNNNNNGC 4 cut(s) 299, 957, 1114, 1468
BstNI CCWGG 1 cut(s) 601
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 3 cut(s) 599, 1118, 1479
BstSNI TACGTA 1 cut(s) 760
BstUI CGCG 2 cut(s) 434, 540
BstV1I GCAGC 3 cut(s) 71, 322, 360
BstV2I GAAGAC 1 cut(s) 1303
Bsu15I ATCGAT 1 cut(s) 1502
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 3 cut(s) 972, 1117, 1296
BsuTUI ATCGAT 1 cut(s) 1502
BtgI CCRYGG 1 cut(s) 863
BtsCI GGATG 2 cut(s) 538, 1131
BtsIMutI CAGTG 6 cut(s) 98, 672, 713, 783, 800, 1084
Cac8I GCNNGC 6 cut(s) 89, 322, 1294, 1371, 1391, 1524
CaiI CAGNNNCTG 3 cut(s) 299, 600, 1223
Cfr13I GGNCC 2 cut(s) 597, 1116
ClaI ATCGAT 1 cut(s) 1502
CseI GACGC 1 cut(s) 1200
CviAII CATG 7 cut(s) 352, 660, 776, 864, 1027, 1228, 1286
DdeI CTNAG 8 cut(s) 213, 372, 492, 559, 690, 705, 995, 1049
DpnI GATC 9 cut(s) 163, 282, 521, 720, 1005, 1130, 1476, 1494, 1505
DpnII GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
Eam1104I CTCTTC 1 cut(s) 559
EarI CTCTTC 1 cut(s) 559
Eco105I TACGTA 1 cut(s) 760
Eco130I CCWWGG 1 cut(s) 863
Eco47I GGWCC 1 cut(s) 597
Eco57I CTGAAG 1 cut(s) 1021
Eco88I CYCGRG 2 cut(s) 654, 714
EcoO109I RGGNCCY 2 cut(s) 597, 1116
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 1 cut(s) 863
ErhI CCWWGG 1 cut(s) 863
FaeI CATG 7 cut(s) 355, 663, 779, 867, 1030, 1231, 1289
FaqI GGGAC 1 cut(s) 321
FatI CATG 7 cut(s) 351, 659, 775, 863, 1026, 1227, 1285
FauI CCCGC 1 cut(s) 1476
FbaI TGATCA 3 cut(s) 280, 1128, 1474
Fnu4HI GCNGC 3 cut(s) 85, 311, 349
FokI GGATG 2 cut(s) 545, 1118
Fsp4HI GCNGC 3 cut(s) 85, 311, 349
FspBI CTAG 5 cut(s) 26, 236, 333, 1188, 1523
GluI GCNGC 3 cut(s) 85, 311, 349
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 3 cut(s) 972, 1117, 1296
HapII CCGG 3 cut(s) 245, 1120, 1481
HgaI GACGC 1 cut(s) 1200
Hin1II CATG 7 cut(s) 355, 663, 779, 867, 1030, 1231, 1289
HincII GTYRAC 2 cut(s) 1258, 1455
HindII GTYRAC 2 cut(s) 1258, 1455
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 7 cut(s) 17, 133, 296, 796, 1089, 1336, 1408
HpaII CCGG 3 cut(s) 245, 1120, 1481
HphI GGTGA 5 cut(s) 19, 374, 805, 1469, 1480
Hpy166II GTNNAC 4 cut(s) 711, 783, 1258, 1455
Hpy188I TCNGA 8 cut(s) 853, 1040, 1088, 1094, 1128, 1355, 1399, 1508
Hpy188III TCNNGA 6 cut(s) 245, 716, 816, 1159, 1319, 1604
Hpy8I GTNNAC 4 cut(s) 711, 783, 1258, 1455
Hpy99I CGWCG 1 cut(s) 1217
HpyAV CCTTC 5 cut(s) 396, 1017, 1325, 1404, 1506
HpyCH4III ACNGT 7 cut(s) 102, 515, 804, 903, 967, 1058, 1359
HpyCH4IV ACGT 3 cut(s) 759, 1451, 1557
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 957, 1114, 1468
HpyF3I CTNAG 8 cut(s) 213, 372, 492, 559, 690, 705, 995, 1049
HpySE526I ACGT 3 cut(s) 759, 1451, 1557
Hsp92II CATG 7 cut(s) 355, 663, 779, 867, 1030, 1231, 1289
Kpn2I TCCGGA 1 cut(s) 244
Ksp22I TGATCA 3 cut(s) 280, 1128, 1474
Kzo9I GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
LmnI GCTCC 4 cut(s) 71, 475, 991, 1289
Lsp1109I GCAGC 3 cut(s) 71, 322, 360
LweI GCATC 2 cut(s) 535, 928
MaeI CTAG 5 cut(s) 26, 236, 333, 1188, 1523
MaeII ACGT 3 cut(s) 759, 1451, 1557
MaeIII GTNAC 2 cut(s) 961, 1058
MalI GATC 9 cut(s) 163, 282, 521, 720, 1005, 1130, 1476, 1494, 1505
MboI GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
MboII GAAGA 5 cut(s) 67, 546, 570, 947, 1303
MhlI GDGCHC 2 cut(s) 691, 1451
MmeI TCCRAC 2 cut(s) 135, 1333
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 5 cut(s) 425, 525, 975, 1566, 1595
MslI CAYNNNNRTG 3 cut(s) 774, 788, 1566
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 3 cut(s) 245, 1120, 1481
MspR9I CCNGG 3 cut(s) 601, 1120, 1481
Mva1269I GAATGC 3 cut(s) 74, 324, 1015
MvaI CCWGG 1 cut(s) 601
MvnI CGCG 2 cut(s) 434, 540
MwoI GCNNNNNNNGC 4 cut(s) 299, 957, 1114, 1468
NciI CCSGG 2 cut(s) 1120, 1481
NcoI CCATGG 1 cut(s) 863
NdeII GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
NheI GCTAGC 1 cut(s) 1522
NlaIII CATG 7 cut(s) 355, 663, 779, 867, 1030, 1231, 1289
NlaIV GGNNCC 4 cut(s) 477, 498, 1118, 1361
NspI RCATGY 1 cut(s) 355
OliI CACNNNNGTG 1 cut(s) 788
PaeR7I CTCGAG 2 cut(s) 654, 714
PcsI WCGNNNNNNNCGW 1 cut(s) 918
PctI GAATGC 3 cut(s) 74, 324, 1015
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 7 cut(s) 17, 133, 296, 796, 1089, 1336, 1408
PfoI TCCNGGA 1 cut(s) 599
PkrI GCNGC 3 cut(s) 86, 312, 350
Ple19I CGATCG 1 cut(s) 1506
Ppu21I YACGTR 1 cut(s) 760
PpuMI RGGWCCY 1 cut(s) 597
Psp5II RGGWCCY 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 4 cut(s) 477, 498, 1118, 1361
PspPI GGNCC 2 cut(s) 597, 1116
PspPPI RGGWCCY 1 cut(s) 597
PspXI VCTCGAGB 1 cut(s) 654
PstNI CAGNNNCTG 3 cut(s) 299, 600, 1223
PvuI CGATCG 1 cut(s) 1506
PvuII CAGCTG 1 cut(s) 401
RseI CAYNNNNRTG 3 cut(s) 774, 788, 1566
SaqAI TTAA 5 cut(s) 425, 525, 975, 1566, 1595
SatI GCNGC 3 cut(s) 85, 311, 349
Sau3AI GATC 9 cut(s) 161, 280, 519, 718, 1003, 1128, 1474, 1492, 1503
Sau96I GGNCC 2 cut(s) 597, 1116
ScaI AGTACT 1 cut(s) 652
ScrFI CCNGG 3 cut(s) 601, 1120, 1481
SduI GDGCHC 2 cut(s) 691, 1451
SfaNI GCATC 2 cut(s) 535, 928
Sfr274I CTCGAG 2 cut(s) 654, 714
SinI GGWCC 1 cut(s) 597
SlaI CTCGAG 2 cut(s) 654, 714
SmiMI CAYNNNNRTG 3 cut(s) 774, 788, 1566
SmlI CTYRAG 4 cut(s) 654, 714, 1412, 1604
SmoI CTYRAG 4 cut(s) 654, 714, 1412, 1604
SnaBI TACGTA 1 cut(s) 760
SpeI ACTAGT 1 cut(s) 332
SsiI CCGC 2 cut(s) 5, 1469
SspMI CTAG 5 cut(s) 26, 236, 333, 1188, 1523
StyD4I CCNGG 3 cut(s) 599, 1118, 1479
StyI CCWWGG 1 cut(s) 863
TaaI ACNGT 7 cut(s) 102, 515, 804, 903, 967, 1058, 1359
TaiI ACGT 3 cut(s) 762, 1454, 1560
TaqI TCGA 7 cut(s) 20, 622, 655, 715, 817, 921, 1502
TatI WGTACW 4 cut(s) 650, 676, 710, 943
TfiI GAWTC 7 cut(s) 17, 133, 296, 796, 1089, 1336, 1408
Tru1I TTAA 5 cut(s) 425, 525, 975, 1566, 1595
Tru9I TTAA 5 cut(s) 425, 525, 975, 1566, 1595
TscAI CASTG 6 cut(s) 105, 672, 713, 790, 807, 1084
TseI GCWGC 3 cut(s) 84, 310, 348
TspDTI ATGAA 8 cut(s) 564, 872, 1008, 1043, 1244, 1274, 1421, 1488
TspRI CASTG 6 cut(s) 105, 672, 713, 790, 807, 1084
VpaK11BI GGWCC 1 cut(s) 597
XapI RAATTY 5 cut(s) 239, 271, 469, 585, 1241
XceI RCATGY 1 cut(s) 355
XhoI CTCGAG 2 cut(s) 654, 714
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 5 cut(s) 26, 236, 333, 1188, 1523
ZrmI AGTACT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.