MD07G1036300.v1.1

Annexin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001318
Physical Location & Seq
Reverse (-)
2998994 .. 3002547
3554 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1036300.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGCAAGCCTCATTGCTCCAGACCACTTCTCTCCTCACGACGATGCAGAAGCCCTCAGAAACGCATGCAAAGAGCAAGAGCACAAGACAGACACTAGTAAGAGAGGACTCAAACCTAGAAAATTATCTCCAACAATGGCTACCCTAATTGCTCCTGAAAACTTCTCTCCCGTCGACGATGCTGAAGTTCTTCGAAAATCTGCGAAAGGATGGGGGACTGATGAGAAGGCTATACTCTCTATACTTGGACATAGAAACGCAGCACAGAGAAAGCAAATCAGGCTTGCTTACGAGCAATCTTATCAGGAAGATCTTCTCAAGCGCCTTGAGTCCGAGCTCTCTGGGGATTTTGAGAAAGCGGTGTACCGTTGGATATTGGATCCTGCAGATCGAGATGCTGTTTTGGCTCATGTGGCCATCAAGAAATCTGAGTGTAACTACAATGTCATCATCGAGATTGCTACCATTCTATCTCCTGAAGAGCTCCTTGCAGTAAGGAAGGCTTACCAAGTTCGCTACAAGCACTCCTTGGAGGAAGACTTGGCTGCACACACCACTGGCGATGTCCGCAAGCTTTTGGTTGCTTTAGTGACTGCGTATCGTTATGATGGTTCTGAGATCAATGCAAAACTGGCGAATTCGGAAGCTGATATCATTCATGATGCTATCAAAGACAAGGCTTTCAATCATGATGAAGTTATTAGGATCCTAAGTACTAGGAGCAAGACTCAGCTCATGGCAACCTTTAACCGCTACCGAGATGACCATGGCACTTCCATCAGTAAGAGTTTGTTGGAAGAGGGTTGTGATGGTCTCCAGAAGGCACTGCATGTAGCCATCCGATGCCTCAATGACCCCAAGAAGTACTTTGAGAAGGTTCTTCGCAACGCAATCAAGAGGGTTGGAACTGATGAGGATGCTCTCACTCGTGTGATTGTGACAAGGTCCGAGAGGGACTTGAAAGACATCAAAGAGGTTTACTACAAGAAGAACAGCGTTCCTCTCGACCATGCTGTGGCCAAAGAAACTTCCGGGGATTACAAGGACTTCCTCCTTACTCTGCTGGGGAAGGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Annexin

Protein Analysis

360

Amino Acids

40.6

Weight (kDa)

6.66

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Annexin PF00191 60 - 121 8.5e-23 Annexin
Annexin PF00191 132 - 196 2.4e-15 Annexin
Annexin PF00191 215 - 276 1.1e-09 Annexin
Annexin PF00191 289 - 354 9.1e-19 Annexin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1015
AccI GTMKAC 1 cut(s) 174
AciI CCGC 3 cut(s) 359, 568, 751
AclWI GGATC 4 cut(s) 374, 387, 700, 713
AcoI YGGCCR 2 cut(s) 414, 1017
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 2 cut(s) 204, 498
AfaI GTAC 3 cut(s) 365, 715, 866
AfiI CCNNNNNNNGG 1 cut(s) 1015
AgsI TTSAA 2 cut(s) 685, 961
AhlI ACTAGT 1 cut(s) 95
AleI CACNNNNGTG 1 cut(s) 929
AluBI AGCT 5 cut(s) 337, 484, 574, 647, 733
AluI AGCT 5 cut(s) 337, 484, 574, 647, 733
Alw21I GWGCWC 3 cut(s) 84, 339, 486
Alw26I GTCTC 1 cut(s) 818
AlwI GGATC 4 cut(s) 374, 387, 700, 713
AoxI GGCC 2 cut(s) 414, 1017
ApeKI GCWGC 2 cut(s) 260, 545
ApoI RAATTY 1 cut(s) 637
Asp700I GAANNNNTTC 2 cut(s) 189, 312
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 945
AsuC2I CCSGG 1 cut(s) 1033
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 945
BalI TGGCCA 2 cut(s) 416, 1019
BamHI GGATCC 2 cut(s) 379, 705
BanII GRGCYC 2 cut(s) 339, 486
BauI CACGAG 1 cut(s) 927
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 3 cut(s) 84, 339, 486
BbvI GCAGC 2 cut(s) 272, 532
BccI CCATC 6 cut(s) 204, 425, 602, 785, 803, 845
BcnI CCSGG 1 cut(s) 1033
BcoDI GTCTC 1 cut(s) 818
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 3 cut(s) 96, 117, 717
BfmI CTRYAG 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 325
BglII AGATCT 1 cut(s) 310
BisI GCNGC 2 cut(s) 261, 546
BlsI GCNGC 2 cut(s) 262, 547
BmcAI AGTACT 2 cut(s) 715, 866
Bme1390I CCNGG 1 cut(s) 1033
Bme18I GGWCC 1 cut(s) 945
BmgT120I GGNCC 1 cut(s) 945
BmiI GGNNCC 2 cut(s) 381, 707
BmrFI CCNGG 1 cut(s) 1033
BmsI GCATC 6 cut(s) 34, 169, 385, 652, 833, 907
BpiI GAAGAC 1 cut(s) 543
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 193
BpuEI CTTGAG 2 cut(s) 302, 347
BpuMI CCSGG 1 cut(s) 1033
BsaI GGTCTC 1 cut(s) 818
BsaJI CCNNGG 3 cut(s) 528, 766, 1032
BsaXI ACNNNNNCTCC 2 cut(s) 509, 539
Bsc4I CCNNNNNNNGG 1 cut(s) 1015
Bse1I ACTGG 2 cut(s) 562, 636
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 3 cut(s) 528, 766, 1032
BseGI GGATG 3 cut(s) 215, 837, 922
BseLI CCNNNNNNNGG 1 cut(s) 1015
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 4 cut(s) 70, 420, 606, 743
BseNI ACTGG 2 cut(s) 562, 636
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 2 cut(s) 272, 532
BseYI CCCAGC 1 cut(s) 1063
BsgI GTGCAG 1 cut(s) 531
BshFI GGCC 2 cut(s) 416, 1019
BsiHKAI GWGCWC 3 cut(s) 84, 339, 486
BsiSI CCGG 1 cut(s) 1032
BslFI GGGAC 2 cut(s) 229, 968
BslI CCNNNNNNNGG 1 cut(s) 1015
BsmAI GTCTC 1 cut(s) 818
BsmFI GGGAC 2 cut(s) 229, 968
BsnI GGCC 2 cut(s) 416, 1019
Bso31I GGTCTC 1 cut(s) 818
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 3 cut(s) 84, 339, 486
Bsp143I GATC 5 cut(s) 310, 379, 388, 618, 705
Bsp19I CCATGG 1 cut(s) 766
BspACI CCGC 3 cut(s) 359, 568, 751
BspANI GGCC 2 cut(s) 416, 1019
BspCNI CTCAG 4 cut(s) 69, 421, 607, 742
BspHI TCATGA 2 cut(s) 658, 688
BspLI GGNNCC 2 cut(s) 381, 707
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 4 cut(s) 374, 387, 700, 713
BspQI GCTCTTC 1 cut(s) 474
BspT104I TTCGAA 1 cut(s) 193
BspTNI GGTCTC 1 cut(s) 818
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 2 cut(s) 562, 636
BssECI CCNNGG 3 cut(s) 528, 766, 1032
BssMI GATC 5 cut(s) 310, 379, 388, 618, 705
BssSI CACGAG 1 cut(s) 927
BssT1I CCWWGG 2 cut(s) 528, 766
Bst2BI CACGAG 1 cut(s) 927
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 2 cut(s) 474, 792
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 4 cut(s) 7, 67, 285, 572
BstDEI CTNAG 5 cut(s) 56, 429, 615, 710, 729
BstDSI CCRYGG 1 cut(s) 766
BstF5I GGATG 3 cut(s) 215, 837, 922
BstH2I RGCGCY 1 cut(s) 325
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 5 cut(s) 313, 382, 391, 621, 708
BstMAI GTCTC 1 cut(s) 818
BstMBI GATC 5 cut(s) 310, 379, 388, 618, 705
BstMWI GCNNNNNNNGC 5 cut(s) 280, 404, 413, 567, 632
BstNSI RCATGY 2 cut(s) 69, 833
BstSCI CCNGG 1 cut(s) 1031
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 272, 532
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 3 cut(s) 310, 379, 705
BstYI RGATCY 3 cut(s) 310, 379, 705
BsuRI GGCC 2 cut(s) 416, 1019
BtgI CCRYGG 1 cut(s) 766
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 3 cut(s) 215, 837, 922
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 2 cut(s) 555, 824
Cac8I GCNNGC 4 cut(s) 7, 67, 285, 572
CciI TCATGA 2 cut(s) 658, 688
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 945
Csp6I GTAC 3 cut(s) 364, 714, 865
CviAII CATG 8 cut(s) 66, 410, 659, 689, 736, 767, 830, 1010
CviQI GTAC 3 cut(s) 364, 714, 865
DdeI CTNAG 5 cut(s) 56, 429, 615, 710, 729
DpnI GATC 5 cut(s) 312, 381, 390, 620, 707
DpnII GATC 5 cut(s) 310, 379, 388, 618, 705
EaeI YGGCCR 2 cut(s) 414, 1017
Eam1104I CTCTTC 2 cut(s) 474, 792
EarI CTCTTC 2 cut(s) 474, 792
Ecl136II GAGCTC 2 cut(s) 337, 484
Eco130I CCWWGG 2 cut(s) 528, 766
Eco24I GRGCYC 2 cut(s) 339, 486
Eco31I GGTCTC 1 cut(s) 818
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 945
Eco53kI GAGCTC 2 cut(s) 337, 484
Eco57I CTGAAG 2 cut(s) 204, 498
EcoICRI GAGCTC 2 cut(s) 337, 484
EcoRI GAATTC 1 cut(s) 637
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 528, 766
EcoT38I GRGCYC 2 cut(s) 339, 486
ErhI CCWWGG 2 cut(s) 528, 766
FaeI CATG 8 cut(s) 69, 413, 662, 692, 739, 770, 833, 1013
FalI AAGNNNNNCTT 8 cut(s) 471, 503, 487, 519, 512, 544, 851, 883
FaqI GGGAC 2 cut(s) 229, 968
FatI CATG 8 cut(s) 65, 409, 658, 688, 735, 766, 829, 1009
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 261, 546
FokI GGATG 3 cut(s) 222, 824, 929
FriOI GRGCYC 2 cut(s) 339, 486
Fsp4HI GCNGC 2 cut(s) 261, 546
FspBI CTAG 3 cut(s) 96, 117, 717
GlaI GCGC 1 cut(s) 323
GluI GCNGC 2 cut(s) 261, 546
GsaI CCCAGC 1 cut(s) 1067
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 1 cut(s) 325
HaeIII GGCC 2 cut(s) 416, 1019
HapII CCGG 1 cut(s) 1032
HhaI GCGC 1 cut(s) 324
Hin1II CATG 8 cut(s) 69, 413, 662, 692, 739, 770, 833, 1013
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 3 cut(s) 108, 329, 727
HpaII CCGG 1 cut(s) 1032
Hpy166II GTNNAC 3 cut(s) 175, 364, 979
Hpy188I TCNGA 7 cut(s) 59, 334, 430, 616, 643, 842, 949
Hpy8I GTNNAC 3 cut(s) 175, 364, 979
Hpy99I CGWCG 3 cut(s) 44, 176, 179
HpyAV CCTTC 5 cut(s) 220, 493, 814, 868, 1063
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4V TGCA 7 cut(s) 47, 69, 386, 491, 548, 626, 829
HpyF10VI GCNNNNNNNGC 5 cut(s) 280, 404, 413, 567, 632
HpyF3I CTNAG 5 cut(s) 56, 429, 615, 710, 729
Hsp92II CATG 8 cut(s) 69, 413, 662, 692, 739, 770, 833, 1013
HspAI GCGC 1 cut(s) 322
Kzo9I GATC 5 cut(s) 310, 379, 388, 618, 705
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 4 cut(s) 22, 157, 489, 720
Lsp1109I GCAGC 2 cut(s) 272, 532
LweI GCATC 6 cut(s) 34, 169, 385, 652, 833, 907
MaeI CTAG 3 cut(s) 96, 117, 717
MaeIII GTNAC 3 cut(s) 434, 589, 937
MalI GATC 5 cut(s) 312, 381, 390, 620, 707
MboI GATC 5 cut(s) 310, 379, 388, 618, 705
MboII GAAGA 8 cut(s) 182, 305, 320, 491, 548, 809, 872, 1000
MflI RGATCY 3 cut(s) 310, 379, 705
MhlI GDGCHC 3 cut(s) 84, 339, 486
MlsI TGGCCA 2 cut(s) 416, 1019
MluCI AATT 3 cut(s) 122, 147, 637
MluNI TGGCCA 2 cut(s) 416, 1019
MlyI GAGTC 3 cut(s) 102, 338, 721
MmeI TCCRAC 4 cut(s) 155, 350, 774, 883
Mox20I TGGCCA 2 cut(s) 416, 1019
MroXI GAANNNNTTC 2 cut(s) 189, 312
MscI TGGCCA 2 cut(s) 416, 1019
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 2 cut(s) 42, 929
Msp20I TGGCCA 2 cut(s) 416, 1019
MspI CCGG 1 cut(s) 1032
MspR9I CCNGG 1 cut(s) 1033
MwoI GCNNNNNNNGC 5 cut(s) 280, 404, 413, 567, 632
NciI CCSGG 1 cut(s) 1033
NcoI CCATGG 1 cut(s) 766
NdeII GATC 5 cut(s) 310, 379, 388, 618, 705
NlaIII CATG 8 cut(s) 69, 413, 662, 692, 739, 770, 833, 1013
NlaIV GGNNCC 2 cut(s) 381, 707
NmuCI GTSAC 2 cut(s) 589, 937
NspI RCATGY 2 cut(s) 69, 833
NspV TTCGAA 1 cut(s) 193
OliI CACNNNNGTG 1 cut(s) 929
PaeI GCATGC 1 cut(s) 69
PagI TCATGA 2 cut(s) 658, 688
PciSI GCTCTTC 1 cut(s) 474
PdmI GAANNNNTTC 2 cut(s) 189, 312
PflFI GACNNNGTC 1 cut(s) 943
PflMI CCANNNNNTGG 1 cut(s) 1015
PkrI GCNGC 2 cut(s) 262, 547
PleI GAGTC 3 cut(s) 102, 337, 721
PpsI GAGTC 3 cut(s) 102, 337, 721
Psp124BI GAGCTC 2 cut(s) 339, 486
PspFI CCCAGC 1 cut(s) 1063
PspN4I GGNNCC 2 cut(s) 381, 707
PspPI GGNCC 1 cut(s) 945
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 3 cut(s) 310, 379, 705
PsyI GACNNNGTC 1 cut(s) 943
RsaI GTAC 3 cut(s) 365, 715, 866
RsaNI GTAC 3 cut(s) 364, 714, 865
RseI CAYNNNNRTG 2 cut(s) 42, 929
SacI GAGCTC 2 cut(s) 339, 486
SalI GTCGAC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 2 cut(s) 261, 546
Sau3AI GATC 5 cut(s) 310, 379, 388, 618, 705
Sau96I GGNCC 1 cut(s) 945
ScaI AGTACT 2 cut(s) 715, 866
SchI GAGTC 3 cut(s) 102, 338, 721
ScrFI CCNGG 1 cut(s) 1033
SduI GDGCHC 3 cut(s) 84, 339, 486
SfaNI GCATC 6 cut(s) 34, 169, 385, 652, 833, 907
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 193
SgrDI CGTCGACG 1 cut(s) 173
SinI GGWCC 1 cut(s) 945
SmiMI CAYNNNNRTG 2 cut(s) 42, 929
SmlI CTYRAG 2 cut(s) 317, 326
SmoI CTYRAG 2 cut(s) 317, 326
SpeI ACTAGT 1 cut(s) 95
SphI GCATGC 1 cut(s) 69
Sse9I AATT 3 cut(s) 122, 147, 637
SsiI CCGC 3 cut(s) 359, 568, 751
SspMI CTAG 3 cut(s) 96, 117, 717
SstI GAGCTC 2 cut(s) 339, 486
StyD4I CCNGG 1 cut(s) 1031
StyI CCWWGG 2 cut(s) 528, 766
TaaI ACNGT 1 cut(s) 368
TaqI TCGA 5 cut(s) 174, 193, 391, 453, 1005
TasI AATT 3 cut(s) 122, 147, 637
TatI WGTACW 2 cut(s) 713, 864
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 2 cut(s) 562, 831
TseFI GTSAC 2 cut(s) 589, 937
TseI GCWGC 2 cut(s) 260, 545
Tsp45I GTSAC 2 cut(s) 589, 937
TspDTI ATGAA 2 cut(s) 647, 708
TspRI CASTG 2 cut(s) 562, 831
Tth111I GACNNNGTC 1 cut(s) 943
Van91I CCANNNNNTGG 1 cut(s) 1015
VpaK11BI GGWCC 1 cut(s) 945
XapI RAATTY 1 cut(s) 637
XceI RCATGY 2 cut(s) 69, 833
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 189, 312
XspI CTAG 3 cut(s) 96, 117, 717
ZrmI AGTACT 2 cut(s) 715, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.