Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000529

Physical Location & Seq

Genomic Coordinates Reverse (-)

27459405 .. 27463679

Gene Structure 4275 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1181600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGTCGAAGGGAAGGGCTGACGGGAGCCCAAAGAAACGTTTGGTCACCTCAATTCTTGTTCTGGTGATCATTTGTGGTCTCCTATACTTGTATTCTAAGAAAAATGATTCCTCCACTCTTGAGTATGGCAGTAAGATAAGAAAATTCCGTTCTACTTACTTGGGTGCGGATGAAGATGTTGATGAATCACCTCCCAAACTTGGTGAAGATGAAGAGGATGGTGTTATATTGAAGAGCATACCAGTTTGTGATGACAGACATTCAGAACTGATTCCTTGCTTAGACAGAAACCTAATCTACGAAACAAGGTTGAAGTTGGATCTGTCTGTGATGGAGCATTACGAGAGACACTGCCCAGTGCCTGAAAGGCGATATAACTGTTTGATTCCACCTCCTTCTGGATATAAGATCCCAATCAAGTGGCCTAAAAGCCGAGACGAGGTATGGAAAGCAAATATACCTCATACTCACCTTGCGACCGAGAAGTCTGACCAAAAGTGGATGGTTGTCAAAGGTGAAAAGATTGGATTTCCTGGAGGAGGTACTCACTTCCATTATGGAGCTGGCAAGTACATCGCTTCAATGGCTAATATGCTCAACTTTCCTAAGAACATTTTAAATAATGGAGGAAGGCTCCGAACAGTCCTTGATGTTGGTTGTGGTGTTGCTAGTTTTGGAGGATACCTGCTGTCTTCTGATATTATCGCAATGTCCTTGGCACCTAATGATGTTCATCAAAATCAGATCCAGTTTGCATTGGAGAGAGGAATTCCTGCATATCTTGGTGTTTTAGGGACCAAGAGACTCCCTTACCCAAGTAGATCTTTTGAACTGGCTCATTGTTCTCGCTGTAGGATTGATTGGCTTCAAAGGGATGGGATACTTCTCCTTGAGTTAGATAGGGTACTTAGACCGGGAGGTTATTTTGCCTACTCATCTCCTGAAGCCTATGCACAGGATGAAGAGGACCTTAGAATTTGGAAAGCGATGAGTGCCCTTGTGGAACGGATGTGCTGGAAAATTGCTGCTAAAAGGAACCAAACTGTTATTTGGGTCAAGCCGCTGACTAATGATTGTTACATGGAAAGACCTCCTGGTACTCAACCACCCCTTTGCAGATCGGATGATGATCCAGATGCAGTCTATAATGTGAAAATGGAGGCTTGCATCACGCCGTACTCTGAGCAAAACCATAGAGCAAGAGGAAGTGGTTTGGCTCCTTGGCCTGCTCGATTGACTACGCCTCCACCTCGTCTTGGTGATTTTGGCTATTCCAGCGACATATTTGAAAAGGACATGGAAGTTTGGCAACAACGAGTTGAAAATTACTGGAATCTTTTGAGCCCAAAGATTAGTTCTGACACAATAAGGAATGTGATGGACATGAAGGCAAACCTGGGTTCATTTGCAGGGGCTTTGAAGAACAAAGATGTTTGGGTTATGAATGTGGTGCCAGAAGACGGACCTAACACACTTAAAATAATATATGACAGAGGACTGATAGGCTCAGTACACAACTGGTGTGAAGCCTACTCAACCTACCCACGAACTTATGATCTACTTCATGCTTGGACCATCTTCTCCGACATTGAAAGGAAAGGATGTAGCGGCGTTGACTTGTTAATTGAGATGGATCGCATCTTGAGGCCCAAAGGTTTCATCATTGTGCGTGATAACCGAAGGGTGGTGGAGTTCATAAACAAACATATGAAGGCGTTACACTGGGATGCAGTGGCTACCGCTGATGCGGAGGGAGGCACCGAGAAAGATGATGTGGTGTTTATTATCCAGAAAAAGATTTGGCGTACGAGCGAGAGCTTTAGAAATGTAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

612

Amino Acids

69.47

Weight (kDa)

7.84

Isoelectric Point (pI)

43.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	89 - 598	6e-225	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	216 - 310	3.2e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000529)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca	FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica	MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica	Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis	pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis	RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata	RLG00000009742 RLG00000012463
rosa_multiflora	Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii	Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa	Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha	Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana	Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	484
Acc36I	ACCTGC	1 cut(s)	693
AccB1I	GGYRCC	3 cut(s)	718, 1450, 1757
AciI	CCGC	5 cut(s)	167, 1061, 1608, 1740, 1748
AclI	AACGTT	1 cut(s)	37
AclWI	GGATC	5 cut(s)	327, 403, 739, 1124, 1641
AcsI	RAATTY	3 cut(s)	143, 768, 975
AcuI	CTGAAG	1 cut(s)	963
AfaI	GTAC	7 cut(s)	544, 572, 906, 1099, 1178, 1512, 1807
AfiI	CCNNNNNNNGG	7 cut(s)	200, 398, 439, 539, 1256, 1460, 1684
AgsI	TTSAA	9 cut(s)	232, 313, 582, 830, 869, 1289, 1322, 1420, 1592
AjnI	CCWGG	3 cut(s)	532, 1093, 1395
AjuI	GAANNNNNNNTTGG	2 cut(s)	1339, 1371
AluBI	AGCT	2 cut(s)	563, 1818
AluI	AGCT	2 cut(s)	563, 1818
Alw26I	GTCTC	4 cut(s)	83, 340, 429, 796
AlwI	GGATC	5 cut(s)	327, 403, 739, 1124, 1641
AlwNI	CAGNNNCTG	1 cut(s)	362
AoxI	GGCC	3 cut(s)	422, 1223, 1646
ApeKI	GCWGC	1 cut(s)	1025
ApoI	RAATTY	3 cut(s)	143, 768, 975
Asp700I	GAANNNNTTC	1 cut(s)	270
AspS9I	GGNCC	5 cut(s)	795, 967, 1463, 1572, 1647
AsuC2I	CCSGG	1 cut(s)	915
AsuHPI	GGTGA	7 cut(s)	37, 76, 180, 215, 461, 527, 1271
AvaII	GGWCC	4 cut(s)	795, 967, 1463, 1572
BaeGI	GKGCMC	1 cut(s)	997
BanI	GGYRCC	3 cut(s)	718, 1450, 1757
BanII	GRGCYC	2 cut(s)	29, 1346
BbsI	GAAGAC	2 cut(s)	684, 1464
BbvI	GCAGC	1 cut(s)	1012
BccI	CCATC	7 cut(s)	212, 325, 496, 869, 1372, 1583, 1624
BceAI	ACGGC	1 cut(s)	1159
BcgI	CGANNNNNNTGC	2 cut(s)	556, 590
BciT130I	CCWGG	3 cut(s)	534, 1095, 1397
BciVI	GTATCC	2 cut(s)	674, 873
BclI	TGATCA	1 cut(s)	66
BcnI	CCSGG	1 cut(s)	915
BcoDI	GTCTC	4 cut(s)	83, 340, 429, 796
BfaI	CTAG	1 cut(s)	669
BfmI	CTRYAG	1 cut(s)	850
BfuAI	ACCTGC	1 cut(s)	693
BfuI	GTATCC	2 cut(s)	674, 873
BglI	GCCNNNNNGGC	1 cut(s)	367
BglII	AGATCT	1 cut(s)	821
BisI	GCNGC	3 cut(s)	1026, 1061, 1609
BlsI	GCNGC	3 cut(s)	1027, 1062, 1610
Bme1390I	CCNGG	4 cut(s)	534, 915, 1095, 1397
Bme18I	GGWCC	4 cut(s)	795, 967, 1463, 1572
BmgT120I	GGNCC	5 cut(s)	795, 967, 1463, 1572, 1647
BmiI	GGNNCC	8 cut(s)	26, 635, 720, 796, 1037, 1218, 1452, 1759
BmrFI	CCNGG	4 cut(s)	534, 915, 1095, 1397
BmrI	ACTGGG	2 cut(s)	350, 1732
BmsI	GCATC	5 cut(s)	1126, 1176, 1647, 1717, 1735
BmuI	ACTGGG	2 cut(s)	350, 1732
BpiI	GAAGAC	2 cut(s)	684, 1464
BplI	GAGNNNNNCTC	2 cut(s)	618, 650
BpmI	CTGGAG	1 cut(s)	555
BpuEI	CTTGAG	3 cut(s)	140, 911, 1663
BpuMI	CCSGG	1 cut(s)	915
BsaBI	GATNNNNATC	3 cut(s)	413, 732, 1128
BsaI	GGTCTC	1 cut(s)	83
BsaJI	CCNNGG	3 cut(s)	714, 1220, 1396
BsaXI	ACNNNNNCTCC	2 cut(s)	1228, 1258
Bsc4I	CCNNNNNNNGG	7 cut(s)	200, 398, 439, 539, 1256, 1460, 1684
Bse1I	ACTGG	7 cut(s)	242, 356, 748, 837, 1334, 1523, 1727
Bse3DI	GCAATG	1 cut(s)	714
Bse8I	GATNNNNATC	3 cut(s)	413, 732, 1128
BseBI	CCWGG	3 cut(s)	534, 1095, 1397
BseDI	CCNNGG	3 cut(s)	714, 1220, 1396
BseGI	GGATG	9 cut(s)	175, 223, 507, 880, 964, 1014, 1129, 1607, 1732
BseJI	GATNNNNATC	3 cut(s)	413, 732, 1128
BseLI	CCNNNNNNNGG	7 cut(s)	200, 398, 439, 539, 1256, 1460, 1684
BseMI	GCAATG	1 cut(s)	714
BseMII	CTCAG	2 cut(s)	1173, 1521
BseNI	ACTGG	7 cut(s)	242, 356, 748, 837, 1334, 1523, 1727
BseRI	GAGGAG	1 cut(s)	552
BseSI	GKGCMC	1 cut(s)	997
BseXI	GCAGC	1 cut(s)	1012
Bsh1285I	CGRYCG	1 cut(s)	480
BshFI	GGCC	3 cut(s)	424, 1225, 1648
BshNI	GGYRCC	3 cut(s)	718, 1450, 1757
BsiEI	CGRYCG	1 cut(s)	480
BsiSI	CCGG	1 cut(s)	914
BsiWI	CGTACG	1 cut(s)	1805
BslFI	GGGAC	1 cut(s)	808
BslI	CCNNNNNNNGG	7 cut(s)	200, 398, 439, 539, 1256, 1460, 1684
BsmAI	GTCTC	4 cut(s)	83, 340, 429, 796
BsmBI	CGTCTC	1 cut(s)	429
BsmFI	GGGAC	1 cut(s)	808
BsnI	GGCC	3 cut(s)	424, 1225, 1648
Bso31I	GGTCTC	1 cut(s)	83
Bsp1286I	GDGCHC	3 cut(s)	29, 997, 1346
Bsp143I	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
BspACI	CCGC	5 cut(s)	167, 1061, 1608, 1740, 1748
BspANI	GGCC	3 cut(s)	424, 1225, 1648
BspCNI	CTCAG	2 cut(s)	1174, 1520
BspLI	GGNNCC	8 cut(s)	26, 635, 720, 796, 1037, 1218, 1452, 1759
BspMI	ACCTGC	1 cut(s)	693
BspPI	GGATC	5 cut(s)	327, 403, 739, 1124, 1641
BspQI	GCTCTTC	1 cut(s)	227
BspT107I	GGYRCC	3 cut(s)	718, 1450, 1757
BspTNI	GGTCTC	1 cut(s)	83
BsrDI	GCAATG	1 cut(s)	714
BsrI	ACTGG	7 cut(s)	242, 356, 748, 837, 1334, 1523, 1727
BssECI	CCNNGG	3 cut(s)	714, 1220, 1396
BssMI	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
BssT1I	CCWWGG	2 cut(s)	714, 1220
Bst2UI	CCWGG	3 cut(s)	534, 1095, 1397
Bst4CI	ACNGT	3 cut(s)	380, 643, 1045
Bst6I	CTCTTC	3 cut(s)	207, 227, 957
BstC8I	GCNNGC	3 cut(s)	565, 1165, 1227
BstDEI	CTNAG	7 cut(s)	96, 280, 606, 908, 971, 1182, 1507
BstEII	GGTNACC	1 cut(s)	43
BstENI	CCTNNNNNAGG	1 cut(s)	537
BstF5I	GGATG	9 cut(s)	175, 223, 507, 880, 964, 1014, 1129, 1607, 1732
BstKTI	GATC	9 cut(s)	69, 322, 411, 747, 824, 1121, 1132, 1558, 1636
BstMAI	GTCTC	4 cut(s)	83, 340, 429, 796
BstMBI	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
BstMCI	CGRYCG	1 cut(s)	480
BstMWI	GCNNNNNNNGC	4 cut(s)	367, 584, 992, 1275
BstNI	CCWGG	3 cut(s)	534, 1095, 1397
BstPI	GGTNACC	1 cut(s)	43
BstSCI	CCNGG	4 cut(s)	532, 913, 1093, 1395
BstSFI	CTRYAG	1 cut(s)	850
BstSLI	GKGCMC	1 cut(s)	997
BstV1I	GCAGC	1 cut(s)	1012
BstV2I	GAAGAC	2 cut(s)	684, 1464
BstX2I	RGATCY	4 cut(s)	319, 408, 744, 821
BstXI	CCANNNNNNTGG	1 cut(s)	420
BstYI	RGATCY	4 cut(s)	319, 408, 744, 821
BsuI	GTATCC	2 cut(s)	674, 873
BsuRI	GGCC	3 cut(s)	424, 1225, 1648
BtgZI	GCGATG	2 cut(s)	559, 1001
BtsCI	GGATG	9 cut(s)	175, 223, 507, 880, 964, 1014, 1129, 1607, 1732
BtsI	GCAGTG	2 cut(s)	349, 1737
BtsIMutI	CAGTG	4 cut(s)	349, 363, 1720, 1737
BveI	ACCTGC	1 cut(s)	693
Cac8I	GCNNGC	3 cut(s)	565, 1165, 1227
CaiI	CAGNNNCTG	1 cut(s)	362
Cfr13I	GGNCC	5 cut(s)	795, 967, 1463, 1572, 1647
Csp6I	GTAC	7 cut(s)	543, 571, 905, 1098, 1177, 1511, 1806
CviAII	CATG	4 cut(s)	1081, 1297, 1384, 1565
CviQI	GTAC	7 cut(s)	543, 571, 905, 1098, 1177, 1511, 1806
DdeI	CTNAG	7 cut(s)	96, 280, 606, 908, 971, 1182, 1507
DpnI	GATC	9 cut(s)	68, 321, 410, 746, 823, 1120, 1131, 1557, 1635
DpnII	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
DraI	TTTAAA	1 cut(s)	618
DrdI	GACNNNNNNGTC	1 cut(s)	484
DseDI	GACNNNNNNGTC	1 cut(s)	484
Eam1104I	CTCTTC	3 cut(s)	207, 227, 957
EarI	CTCTTC	3 cut(s)	207, 227, 957
Eco130I	CCWWGG	2 cut(s)	714, 1220
Eco24I	GRGCYC	2 cut(s)	29, 1346
Eco31I	GGTCTC	1 cut(s)	83
Eco47I	GGWCC	4 cut(s)	795, 967, 1463, 1572
Eco57I	CTGAAG	1 cut(s)	963
Eco91I	GGTNACC	1 cut(s)	43
EcoNI	CCTNNNNNAGG	1 cut(s)	537
EcoO109I	RGGNCCY	1 cut(s)	967
EcoO65I	GGTNACC	1 cut(s)	43
EcoRI	GAATTC	1 cut(s)	768
EcoRII	CCWGG	3 cut(s)	532, 1093, 1395
EcoT14I	CCWWGG	2 cut(s)	714, 1220
EcoT38I	GRGCYC	2 cut(s)	29, 1346
ErhI	CCWWGG	2 cut(s)	714, 1220
Esp3I	CGTCTC	1 cut(s)	429
FaeI	CATG	4 cut(s)	1084, 1300, 1387, 1568
FalI	AAGNNNNNCTT	4 cut(s)	808, 840, 954, 986
FaqI	GGGAC	1 cut(s)	808
FatI	CATG	4 cut(s)	1080, 1296, 1383, 1564
FauNDI	CATATG	1 cut(s)	1707
FbaI	TGATCA	1 cut(s)	66
Fnu4HI	GCNGC	3 cut(s)	1026, 1061, 1609
FokI	GGATG	9 cut(s)	182, 230, 514, 887, 971, 1021, 1136, 1614, 1739
FriOI	GRGCYC	2 cut(s)	29, 1346
Fsp4HI	GCNGC	3 cut(s)	1026, 1061, 1609
FspBI	CTAG	1 cut(s)	669
GluI	GCNGC	3 cut(s)	1026, 1061, 1609
GsuI	CTGGAG	1 cut(s)	555
HaeIII	GGCC	3 cut(s)	424, 1225, 1648
HapII	CCGG	1 cut(s)	914
Hin1II	CATG	4 cut(s)	1084, 1300, 1387, 1568
HincII	GTYRAC	1 cut(s)	1615
HindII	GTYRAC	1 cut(s)	1615
HinfI	GANTC	6 cut(s)	107, 185, 271, 385, 804, 1333
HpaII	CCGG	1 cut(s)	914
HphI	GGTGA	7 cut(s)	37, 76, 180, 215, 461, 527, 1271
Hpy166II	GTNNAC	2 cut(s)	1513, 1615
Hpy188I	TCNGA	9 cut(s)	265, 490, 638, 697, 744, 1123, 1183, 1360, 1585
Hpy188III	TCNNGA	6 cut(s)	119, 399, 941, 1133, 1642, 1789
Hpy8I	GTNNAC	2 cut(s)	1513, 1615
HpyAV	CCTTC	6 cut(s)	6, 405, 624, 1381, 1674, 1705
HpyCH4III	ACNGT	3 cut(s)	380, 643, 1045
HpyCH4IV	ACGT	1 cut(s)	37
HpyCH4V	TGCA	8 cut(s)	755, 776, 953, 1116, 1139, 1167, 1409, 1730
HpyF10VI	GCNNNNNNNGC	4 cut(s)	367, 584, 992, 1275
HpyF3I	CTNAG	7 cut(s)	96, 280, 606, 908, 971, 1182, 1507
HpySE526I	ACGT	1 cut(s)	37
Hsp92II	CATG	4 cut(s)	1084, 1300, 1387, 1568
Ksp22I	TGATCA	1 cut(s)	66
Kzo9I	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
LguI	GCTCTTC	1 cut(s)	227
LmnI	GCTCC	5 cut(s)	24, 334, 560, 639, 1222
Lsp1109I	GCAGC	1 cut(s)	1012
LweI	GCATC	5 cut(s)	1126, 1176, 1647, 1717, 1735
MaeI	CTAG	1 cut(s)	669
MaeII	ACGT	1 cut(s)	37
MaeIII	GTNAC	3 cut(s)	43, 1076, 1716
MalI	GATC	9 cut(s)	68, 321, 410, 746, 823, 1120, 1131, 1557, 1635
MboI	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
MboII	GAAGA	9 cut(s)	185, 218, 224, 244, 684, 974, 1432, 1469, 1570
MflI	RGATCY	4 cut(s)	319, 408, 744, 821
MhlI	GDGCHC	3 cut(s)	29, 997, 1346
MluCI	AATT	7 cut(s)	51, 143, 768, 975, 1020, 1324, 1623
MlyI	GAGTC	1 cut(s)	798
MmeI	TCCRAC	2 cut(s)	297, 1608
MroXI	GAANNNNTTC	1 cut(s)	270
MseI	TTAA	3 cut(s)	617, 1476, 1622
MslI	CAYNNNNRTG	2 cut(s)	1664, 1725
MspA1I	CMGCKG	2 cut(s)	1063, 1742
MspI	CCGG	1 cut(s)	914
MspR9I	CCNGG	4 cut(s)	534, 915, 1095, 1397
MvaI	CCWGG	3 cut(s)	534, 1095, 1397
MwoI	GCNNNNNNNGC	4 cut(s)	367, 584, 992, 1275
NciI	CCSGG	1 cut(s)	915
NdeI	CATATG	1 cut(s)	1707
NdeII	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
NlaIII	CATG	4 cut(s)	1084, 1300, 1387, 1568
NlaIV	GGNNCC	8 cut(s)	26, 635, 720, 796, 1037, 1218, 1452, 1759
NmeAIII	GCCGAG	1 cut(s)	458
NmuCI	GTSAC	1 cut(s)	43
PciSI	GCTCTTC	1 cut(s)	227
PdmI	GAANNNNTTC	1 cut(s)	270
PfeI	GAWTC	5 cut(s)	107, 185, 271, 385, 1333
Pfl23II	CGTACG	1 cut(s)	1805
PfoI	TCCNGGA	1 cut(s)	532
PkrI	GCNGC	3 cut(s)	1027, 1062, 1610
PleI	GAGTC	1 cut(s)	798
PpsI	GAGTC	1 cut(s)	798
PpuMI	RGGWCCY	1 cut(s)	967
Psp1406I	AACGTT	1 cut(s)	37
Psp5II	RGGWCCY	1 cut(s)	967
Psp6I	CCWGG	3 cut(s)	532, 1093, 1395
PspEI	GGTNACC	1 cut(s)	43
PspGI	CCWGG	3 cut(s)	532, 1093, 1395
PspLI	CGTACG	1 cut(s)	1805
PspN4I	GGNNCC	8 cut(s)	26, 635, 720, 796, 1037, 1218, 1452, 1759
PspPI	GGNCC	5 cut(s)	795, 967, 1463, 1572, 1647
PspPPI	RGGWCCY	1 cut(s)	967
PstNI	CAGNNNCTG	1 cut(s)	362
PsuI	RGATCY	4 cut(s)	319, 408, 744, 821
RsaI	GTAC	7 cut(s)	544, 572, 906, 1099, 1178, 1512, 1807
RsaNI	GTAC	7 cut(s)	543, 571, 905, 1098, 1177, 1511, 1806
RseI	CAYNNNNRTG	2 cut(s)	1664, 1725
SapI	GCTCTTC	1 cut(s)	227
SaqAI	TTAA	3 cut(s)	617, 1476, 1622
SatI	GCNGC	3 cut(s)	1026, 1061, 1609
Sau3AI	GATC	9 cut(s)	66, 319, 408, 744, 821, 1118, 1129, 1555, 1633
Sau96I	GGNCC	5 cut(s)	795, 967, 1463, 1572, 1647
SchI	GAGTC	1 cut(s)	798
ScrFI	CCNGG	4 cut(s)	534, 915, 1095, 1397
SduI	GDGCHC	3 cut(s)	29, 997, 1346
SfaNI	GCATC	5 cut(s)	1126, 1176, 1647, 1717, 1735
SfcI	CTRYAG	1 cut(s)	850
SinI	GGWCC	4 cut(s)	795, 967, 1463, 1572
SmiMI	CAYNNNNRTG	2 cut(s)	1664, 1725
SmlI	CTYRAG	3 cut(s)	119, 890, 1642
SmoI	CTYRAG	3 cut(s)	119, 890, 1642
Sse9I	AATT	7 cut(s)	51, 143, 768, 975, 1020, 1324, 1623
SsiI	CCGC	5 cut(s)	167, 1061, 1608, 1740, 1748
SspMI	CTAG	1 cut(s)	669
StyD4I	CCNGG	4 cut(s)	532, 913, 1093, 1395
StyI	CCWWGG	2 cut(s)	714, 1220
TaaI	ACNGT	3 cut(s)	380, 643, 1045
TaiI	ACGT	1 cut(s)	40
TaqI	TCGA	2 cut(s)	5, 1231
TaqII	GACCGA	1 cut(s)	494
TasI	AATT	7 cut(s)	51, 143, 768, 975, 1020, 1324, 1623
TatI	WGTACW	2 cut(s)	570, 1510
TauI	GCSGC	2 cut(s)	1063, 1611
TfiI	GAWTC	5 cut(s)	107, 185, 271, 385, 1333
Tru1I	TTAA	3 cut(s)	617, 1476, 1622
Tru9I	TTAA	3 cut(s)	617, 1476, 1622
TscAI	CASTG	4 cut(s)	356, 363, 1727, 1737
TseFI	GTSAC	1 cut(s)	43
TseI	GCWGC	1 cut(s)	1025
Tsp45I	GTSAC	1 cut(s)	43
TspGWI	ACGGA	3 cut(s)	137, 1021, 1476
TspRI	CASTG	4 cut(s)	356, 363, 1727, 1737
VpaK11BI	GGWCC	4 cut(s)	795, 967, 1463, 1572
XagI	CCTNNNNNAGG	1 cut(s)	537
XapI	RAATTY	3 cut(s)	143, 768, 975
XcmI	CCANNNNNNNNNTGG	1 cut(s)	560
XmnI	GAANNNNTTC	1 cut(s)	270
XspI	CTAG	1 cut(s)	669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.