Rroxscaffold_5G00338470

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000529
Physical Location & Seq
Forward (+)
6623060 .. 6635755
12696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00338470.1

Sequence Viewer

Length: 642 bp
ATGAAGTATGGAAAGCAAATATACCTCATACTCACCTTGCACATGAGAAATCTGACCAGAACTGGATGGTTGAAAAAGGTGACAAGATTAGTTTTCCTGGGGGAGGCACACATTTTCACTATGGAGCTGATAAGTATATTGCTTCAATTGCAAATGTTACGTACAGTTTTTGACGTTGGCTGTGGAGTTGCAAGTTTTGGAGGCTATCTTCTGTCATCTGATATTATAACAATGTCCTTAGCACCCAATGATGTGCATCAAAATCAAATCCAATTTGCTTTGGAAAGAGGAATTCCAGCATATCTTGGTGTTCTAGGGACCAAAAGGCTTCCTTACCCAAGCAGATCTTTTGAACTTGCTCACTGTTCCCGTTGTAGAATTGATTGGCTCCAAAGAGATGGGATCCTTCTTCTTGAGCTAGATAGGTTACTCAGGCCAGGAGGCTACTTTGCGTACTCATCTCCAGAAGCATATGCACAGGATGAGGAAGATCTCAAAATATGGAAAGACATGAGTGCCCTTGTGGAACGCATGTGTTGGAGAATAGCTGCAAAAAGGAACCAAACTGTCATTTGGCAGAAACCACTAACAAATGACTGTTATATGCAAAGAGAACCTGGCACTCAACCTCCTCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

213

Amino Acids

24.54

Weight (kDa)

8.9

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 46 - 213 4.1e-81 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 58 - 151 9.1e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AclWI GGATC 2 cut(s) 397, 410
AcsI RAATTY 1 cut(s) 291
AfaI GTAC 2 cut(s) 163, 455
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 3 cut(s) 73, 146, 353
AjnI CCWGG 3 cut(s) 96, 436, 616
AluBI AGCT 3 cut(s) 127, 418, 548
AluI AGCT 3 cut(s) 127, 418, 548
AlwI GGATC 2 cut(s) 397, 410
AoxI GGCC 1 cut(s) 434
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 25, 91
AvaII GGWCC 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 520
BamHI GGATCC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 535
BccI CCATC 2 cut(s) 60, 392
BciT130I CCWGG 3 cut(s) 98, 438, 618
BfaI CTAG 2 cut(s) 314, 419
BglII AGATCT 2 cut(s) 344, 490
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
Bme1390I CCNGG 3 cut(s) 98, 438, 618
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 4 cut(s) 319, 389, 404, 560
BmrFI CCNGG 3 cut(s) 98, 438, 618
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 97
BsaXI ACNNNNNCTCC 1 cut(s) 613
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 67
Bse8I GATNNNNATC 1 cut(s) 255
BseBI CCWGG 3 cut(s) 98, 438, 618
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 2 cut(s) 71, 487
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 445
BseNI ACTGG 1 cut(s) 67
BseRI GAGGAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 1 cut(s) 535
BshFI GGCC 1 cut(s) 436
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 3 cut(s) 344, 402, 490
BspANI GGCC 1 cut(s) 436
BspCNI CTCAG 1 cut(s) 444
BspLI GGNNCC 4 cut(s) 319, 389, 404, 560
BspPI GGATC 2 cut(s) 397, 410
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 3 cut(s) 344, 402, 490
Bst2UI CCWGG 3 cut(s) 98, 438, 618
Bst4CI ACNGT 4 cut(s) 166, 365, 568, 599
BstBAI YACGTR 1 cut(s) 161
BstDEI CTNAG 2 cut(s) 238, 431
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 71, 487
BstKTI GATC 3 cut(s) 347, 405, 493
BstMBI GATC 3 cut(s) 344, 402, 490
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNI CCWGG 3 cut(s) 98, 438, 618
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 3 cut(s) 96, 436, 616
BstSLI GKGCMC 1 cut(s) 520
BstSNI TACGTA 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 535
BstX2I RGATCY 3 cut(s) 344, 402, 490
BstXI CCANNNNNNTGG 1 cut(s) 398
BstYI RGATCY 3 cut(s) 344, 402, 490
BsuRI GGCC 1 cut(s) 436
BtsCI GGATG 2 cut(s) 71, 487
BtsIMutI CAGTG 1 cut(s) 361
Cfr13I GGNCC 1 cut(s) 318
Csp6I GTAC 2 cut(s) 162, 454
CviAII CATG 3 cut(s) 43, 511, 532
CviJI RGCY 9 cut(s) 127, 180, 204, 328, 388, 418, 436, 444, 548
CviKI_1 RGCY 9 cut(s) 127, 180, 204, 328, 388, 418, 436, 444, 548
CviQI GTAC 2 cut(s) 162, 454
DdeI CTNAG 2 cut(s) 238, 431
DpnI GATC 3 cut(s) 346, 404, 492
DpnII GATC 3 cut(s) 344, 402, 490
Eco105I TACGTA 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 3 cut(s) 96, 436, 616
FaeI CATG 3 cut(s) 46, 514, 535
FalI AAGNNNNNCTT 4 cut(s) 316, 348, 331, 363
FaqI GGGAC 1 cut(s) 331
FatI CATG 3 cut(s) 42, 510, 531
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 2 cut(s) 78, 494
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 2 cut(s) 314, 419
GluI GCNGC 1 cut(s) 549
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 1 cut(s) 436
Hin1II CATG 3 cut(s) 46, 514, 535
HphI GGTGA 2 cut(s) 25, 91
Hpy188I TCNGA 2 cut(s) 54, 220
Hpy188III TCNNGA 2 cut(s) 413, 464
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 4 cut(s) 166, 365, 568, 599
HpyCH4IV ACGT 2 cut(s) 160, 174
HpyCH4V TGCA 7 cut(s) 40, 151, 191, 256, 476, 551, 607
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 2 cut(s) 238, 431
HpySE526I ACGT 2 cut(s) 160, 174
Hsp92II CATG 3 cut(s) 46, 514, 535
Kzo9I GATC 3 cut(s) 344, 402, 490
LmnI GCTCC 2 cut(s) 124, 393
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 314, 419
MaeII ACGT 2 cut(s) 160, 174
MaeIII GTNAC 3 cut(s) 79, 156, 426
MalI GATC 3 cut(s) 346, 404, 492
MboI GATC 3 cut(s) 344, 402, 490
MboII GAAGA 3 cut(s) 200, 401, 500
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 3 cut(s) 344, 402, 490
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 4 cut(s) 146, 272, 291, 378
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 8 cut(s) 35, 97, 194, 281, 434, 478, 639, 642
MspR9I CCNGG 3 cut(s) 98, 438, 618
MunI CAATTG 1 cut(s) 146
MvaI CCWGG 3 cut(s) 98, 438, 618
MwoI GCNNNNNNNGC 1 cut(s) 148
NdeI CATATG 1 cut(s) 472
NdeII GATC 3 cut(s) 344, 402, 490
NlaIII CATG 3 cut(s) 46, 514, 535
NlaIV GGNNCC 4 cut(s) 319, 389, 404, 560
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 1 cut(s) 535
PkrI GCNGC 1 cut(s) 550
Ppu21I YACGTR 1 cut(s) 161
PsiI TTATAA 1 cut(s) 227
Psp6I CCWGG 3 cut(s) 96, 436, 616
PspGI CCWGG 3 cut(s) 96, 436, 616
PspN4I GGNNCC 4 cut(s) 319, 389, 404, 560
PspPI GGNCC 1 cut(s) 318
PsuI RGATCY 3 cut(s) 344, 402, 490
RsaI GTAC 2 cut(s) 163, 455
RsaNI GTAC 2 cut(s) 162, 454
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 3 cut(s) 344, 402, 490
Sau96I GGNCC 1 cut(s) 318
ScrFI CCNGG 3 cut(s) 98, 438, 618
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 1 cut(s) 265
SinI GGWCC 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SnaBI TACGTA 1 cut(s) 161
Sse9I AATT 4 cut(s) 146, 272, 291, 378
SspMI CTAG 2 cut(s) 314, 419
StyD4I CCNGG 3 cut(s) 96, 436, 616
TaaI ACNGT 4 cut(s) 166, 365, 568, 599
TaiI ACGT 2 cut(s) 163, 177
TasI AATT 4 cut(s) 146, 272, 291, 378
TscAI CASTG 1 cut(s) 368
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 318
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 291
XceI RCATGY 1 cut(s) 535
XspI CTAG 2 cut(s) 314, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.