Rh4DG045000

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000529
Physical Location & Seq
Forward (+)
7405823 .. 7407837
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG045000.1

Sequence Viewer

Length: 1095 bp
ATGATGAGGGGAAGATCTGATCCAGCCCAAAAGAAGCGCGTGATCACCATTCTGTGTATTTTGGCACTCTTTCGTGTTTTCCTGTATGGGTATTATGGTTCCATCTTTGGCTCTCAAAGTCATGGTGCATCAGCTCTAGAATATGGCAGTAGATCTTTGAGAAAGCTTGGTTCATCTTATTTGGGTGGGGATGATGATACCGATGGCAAGCTGGATGAATCTTCAACAAAGTATGGGCAGGAAGATGGAGATGATGATGTTACAGTGAAGAGCTTCCCGGTTTGTGATGATCGCCATTCGGAACTTATCCGTTGCTTGGACAGAAATCTCATATATCAAATGAGACTAAAGTCCCAATCAAATGGCCCCAGAGCAGAGATGAAGTATGGAAAGCAAATATACCTCATACTTACCTTGCACATGAGAAATCTGACCAGAACTGGATGGTTGAAAAAGGTGACAAGATTAGTTTTCCTGGGGGAGGCACACATTTTCACTATGGAGCTGATAAGTATATTGCTTCAATTGCAAATGTTACGTACAGTTTTTGACGTTGGCTGTGGAGTTGCAAGTTTTGGAGGCTATCTTCTGTCATCTGATATTATAACAATGTCCTTAGCACCCAATGATGTGCATCAAAATCAAATCCAATTTGCTTTGGAAAGCGGAATTCCAGCATATCTTGGTGTTCTAGGGACCAAAAGGCTTCCTTACCCAAGCAGATCTTTTGAACTTGCTCACTGTTCCCGTTGTAGAATTGATTGGCTCCAAAGAGATGGGATCCTTCTTCTTGAGCTAGATAGGTTGCTCAGGCCAGGAGGCTACTTTGCGTACTCATCTCCAGAAGCATATGCACAGGATGAGGAAGATCTCAAAATATGGAAAGAGATGAGTGCCCTTGTGGAACGCATGTGTTGGAGAATAGCTGCAAAAAGGAACCAAACTGTCATTTGGCAGAAACCACTAACAAATGACTGTTATATGCAAAGAGAACCTGGCACTCAACCTCCTCTCTGCCGATCTGATGATGATCCAGATGCAATCTGGGGTGTGCCAATGGAAGCTTGCATCTCACCGTACTCTGATCGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

364

Amino Acids

41.37

Weight (kDa)

7.51

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 172 - 363 1.5e-89 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AccII CGCG 1 cut(s) 39
AciI CCGC 1 cut(s) 666
AclWI GGATC 4 cut(s) 14, 775, 788, 1025
AcsI RAATTY 1 cut(s) 669
AfaI GTAC 3 cut(s) 541, 833, 1079
AfiI CCNNNNNNNGG 2 cut(s) 316, 481
AgsI TTSAA 4 cut(s) 225, 451, 524, 731
AjnI CCWGG 3 cut(s) 474, 814, 994
AluBI AGCT 8 cut(s) 134, 166, 211, 273, 505, 796, 926, 1064
AluI AGCT 8 cut(s) 134, 166, 211, 273, 505, 796, 926, 1064
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 4 cut(s) 14, 775, 788, 1025
AoxI GGCC 2 cut(s) 364, 812
ApeKI GCWGC 1 cut(s) 926
ApoI RAATTY 1 cut(s) 669
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 365, 696
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 3 cut(s) 37, 469, 1065
AvaII GGWCC 1 cut(s) 696
BaeGI GKGCMC 1 cut(s) 898
BamHI GGATCC 1 cut(s) 780
BbvI GCAGC 1 cut(s) 913
BccI CCATC 5 cut(s) 110, 197, 239, 438, 770
BciT130I CCWGG 3 cut(s) 476, 816, 996
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 3 cut(s) 137, 692, 797
BglII AGATCT 4 cut(s) 14, 152, 722, 868
BisI GCNGC 1 cut(s) 927
BlsI GCNGC 1 cut(s) 928
Bme1390I CCNGG 4 cut(s) 278, 476, 816, 996
Bme18I GGWCC 1 cut(s) 696
BmgT120I GGNCC 2 cut(s) 365, 696
BmiI GGNNCC 6 cut(s) 100, 367, 697, 767, 782, 938
BmrFI CCNGG 4 cut(s) 278, 476, 816, 996
BmsI GCATC 4 cut(s) 137, 643, 1027, 1077
BoxI GACNNNNGTC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 825
Bpu10I CCTNAGC 2 cut(s) 616, 809
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 539
BsaBI GATNNNNATC 2 cut(s) 633, 1029
BsaJI CCNNGG 1 cut(s) 475
BsaXI ACNNNNNCTCC 2 cut(s) 991, 1021
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 481
Bse1I ACTGG 1 cut(s) 445
Bse8I GATNNNNATC 2 cut(s) 633, 1029
BseBI CCWGG 3 cut(s) 476, 816, 996
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 4 cut(s) 196, 220, 449, 865
BseJI GATNNNNATC 2 cut(s) 633, 1029
BseLI CCNNNNNNNGG 2 cut(s) 316, 481
BseMII CTCAG 1 cut(s) 823
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 1 cut(s) 999
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 1 cut(s) 913
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 366, 814
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 337, 709
BslI CCNNNNNNNGG 2 cut(s) 316, 481
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 2 cut(s) 337, 709
BsnI GGCC 2 cut(s) 366, 814
Bsp1286I GDGCHC 1 cut(s) 898
BspACI CCGC 1 cut(s) 666
BspANI GGCC 2 cut(s) 366, 814
BspCNI CTCAG 1 cut(s) 822
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 6 cut(s) 100, 367, 697, 767, 782, 938
BspPI GGATC 4 cut(s) 14, 775, 788, 1025
BspQI GCTCTTC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 1 cut(s) 475
Bst2UI CCWGG 3 cut(s) 476, 816, 996
Bst4CI ACNGT 6 cut(s) 265, 544, 743, 946, 977, 1077
Bst6I CTCTTC 1 cut(s) 263
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 209, 1066
BstDEI CTNAG 2 cut(s) 616, 809
BstENI CCTNNNNNAGG 1 cut(s) 479
BstF5I GGATG 4 cut(s) 196, 220, 449, 865
BstFNI CGCG 1 cut(s) 39
BstHHI GCGC 1 cut(s) 39
BstMAI GTCTC 1 cut(s) 337
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstNI CCWGG 3 cut(s) 476, 816, 996
BstNSI RCATGY 1 cut(s) 913
BstPAI GACNNNNGTC 1 cut(s) 349
BstSCI CCNGG 4 cut(s) 276, 474, 814, 994
BstSLI GKGCMC 1 cut(s) 898
BstSNI TACGTA 1 cut(s) 539
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 913
BstX2I RGATCY 5 cut(s) 14, 152, 722, 780, 868
BstXI CCANNNNNNTGG 2 cut(s) 362, 776
BstYI RGATCY 5 cut(s) 14, 152, 722, 780, 868
BsuRI GGCC 2 cut(s) 366, 814
BtsCI GGATG 4 cut(s) 196, 220, 449, 865
BtsIMutI CAGTG 2 cut(s) 270, 739
Cac8I GCNNGC 2 cut(s) 209, 1066
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 365, 696
Csp6I GTAC 3 cut(s) 540, 832, 1078
CviAII CATG 3 cut(s) 122, 421, 910
CviQI GTAC 3 cut(s) 540, 832, 1078
DdeI CTNAG 2 cut(s) 616, 809
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco105I TACGTA 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 696
EcoNI CCTNNNNNAGG 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 3 cut(s) 474, 814, 994
FaeI CATG 3 cut(s) 125, 424, 913
FalI AAGNNNNNCTT 4 cut(s) 694, 726, 709, 741
FaqI GGGAC 2 cut(s) 337, 709
FatI CATG 3 cut(s) 121, 420, 909
FauNDI CATATG 1 cut(s) 850
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 927
FokI GGATG 4 cut(s) 203, 227, 456, 872
Fsp4HI GCNGC 1 cut(s) 927
FspBI CTAG 3 cut(s) 137, 692, 797
GlaI GCGC 1 cut(s) 38
GluI GCNGC 1 cut(s) 927
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 2 cut(s) 366, 814
HapII CCGG 1 cut(s) 278
HhaI GCGC 1 cut(s) 39
Hin1II CATG 3 cut(s) 125, 424, 913
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HindIII AAGCTT 2 cut(s) 164, 1062
HinfI GANTC 1 cut(s) 218
HpaII CCGG 1 cut(s) 278
HphI GGTGA 3 cut(s) 37, 469, 1065
Hpy188I TCNGA 6 cut(s) 19, 301, 432, 598, 1024, 1084
Hpy188III TCNNGA 4 cut(s) 137, 791, 842, 1034
HpyAV CCTTC 1 cut(s) 794
HpyCH4III ACNGT 6 cut(s) 265, 544, 743, 946, 977, 1077
HpyCH4IV ACGT 2 cut(s) 538, 552
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
HpyF3I CTNAG 2 cut(s) 616, 809
HpySE526I ACGT 2 cut(s) 538, 552
Hsp92II CATG 3 cut(s) 125, 424, 913
HspAI GCGC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 42
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 502, 771
Lsp1109I GCAGC 1 cut(s) 913
LweI GCATC 4 cut(s) 137, 643, 1027, 1077
MaeI CTAG 3 cut(s) 137, 692, 797
MaeII ACGT 2 cut(s) 538, 552
MaeIII GTNAC 3 cut(s) 259, 457, 534
MboII GAAGA 7 cut(s) 24, 213, 254, 280, 578, 779, 878
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 5 cut(s) 14, 152, 722, 780, 868
MhlI GDGCHC 1 cut(s) 898
MluCI AATT 4 cut(s) 524, 650, 669, 756
MmeI TCCRAC 1 cut(s) 896
MnlI CCTC 7 cut(s) 413, 475, 572, 812, 856, 1017, 1020
MroXI GAANNNNTTC 1 cut(s) 272
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 4 cut(s) 278, 476, 816, 996
MunI CAATTG 1 cut(s) 524
MvaI CCWGG 3 cut(s) 476, 816, 996
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 526
NciI CCSGG 1 cut(s) 278
NdeI CATATG 1 cut(s) 850
NlaIII CATG 3 cut(s) 125, 424, 913
NlaIV GGNNCC 6 cut(s) 100, 367, 697, 767, 782, 938
NmuCI GTSAC 1 cut(s) 457
NspI RCATGY 1 cut(s) 913
PciSI GCTCTTC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 1 cut(s) 928
Ppu21I YACGTR 1 cut(s) 539
PshAI GACNNNNGTC 1 cut(s) 349
PsiI TTATAA 1 cut(s) 605
Psp6I CCWGG 3 cut(s) 474, 814, 994
PspGI CCWGG 3 cut(s) 474, 814, 994
PspN4I GGNNCC 6 cut(s) 100, 367, 697, 767, 782, 938
PspPI GGNCC 2 cut(s) 365, 696
PsuI RGATCY 5 cut(s) 14, 152, 722, 780, 868
RsaI GTAC 3 cut(s) 541, 833, 1079
RsaNI GTAC 3 cut(s) 540, 832, 1078
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 1 cut(s) 927
Sau96I GGNCC 2 cut(s) 365, 696
ScrFI CCNGG 4 cut(s) 278, 476, 816, 996
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 4 cut(s) 137, 643, 1027, 1077
SinI GGWCC 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
SnaBI TACGTA 1 cut(s) 539
Sse9I AATT 4 cut(s) 524, 650, 669, 756
SsiI CCGC 1 cut(s) 666
SspMI CTAG 3 cut(s) 137, 692, 797
StyD4I CCNGG 4 cut(s) 276, 474, 814, 994
TaaI ACNGT 6 cut(s) 265, 544, 743, 946, 977, 1077
TaiI ACGT 2 cut(s) 541, 555
TasI AATT 4 cut(s) 524, 650, 669, 756
TfiI GAWTC 1 cut(s) 218
TscAI CASTG 2 cut(s) 270, 746
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 1 cut(s) 926
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 3 cut(s) 162, 231, 395
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 270, 746
VpaK11BI GGWCC 1 cut(s) 696
XagI CCTNNNNNAGG 1 cut(s) 479
XapI RAATTY 1 cut(s) 669
XbaI TCTAGA 1 cut(s) 136
XceI RCATGY 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 1041
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 3 cut(s) 137, 692, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.