pycom05g18230

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000529
Physical Location & Seq
Reverse (-)
21197050 .. 21198273
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18230.4

Sequence Viewer

Length: 897 bp
ATGTCACGGGGAAGGGCTGACGGGATCCCAAAGAAACGTTTGGTCACCTCAGTTCTTGTTTTGGTGATCATTTGTGGTCTCCTATACTTGTATTCTAAGAAAAATGGTTCCTCCGGTCTCGAGTATGGCAGTAAGATAAGAAAATTCGGTTCTACTTACTTGGGTGCGGATGAAGATGTTGACGAGTCACCTCCCAAACTTGGTGAAGATGAAGAGGATGGTGTTATATTGAAGAGCATACCGGTTTGTGATGATAGACATTCAGAACTGATTCCTTGCTTAGACAGAAACCTTATTTATGAAACAAGGTTGAAGTTGGATCTGTCTGTGATGGAGCATTACGAGAGACACTGCCCAGTGCCTGAAAGGCGATTTAACTGTTTGATTCCACCTCCTCCTGGATATAAGATCCCAATTAAGTGGCCTAAAAGCCGAGACGAGGTATGGAAAGCAAATATACCTCATACGCACCTTGCGACTGAGAAGTCTGACCAAAAGTGGATGGTTGTCAAAGGTGAAAAGATTGGATTTCCTGGAGGAGGTACTCACTTCCATTATGGAGCTGACAAGTACATCGCATCAATGGCTAATATGCTCAACTTTTCTAACAACATTTTGAATAATGGAGGAAGGCTCCGAACGGTCCTTGATGTTGGTTGTGGTGTTGCTAGTTTTGGAGGATACCTGCTTTCTTCTGATATTATCACAATGTCCTTGGCACCTAATGATGTTCATCAAAATCGATCCAGTTTGCATTGGAGAGAGGAATTCCTGCGTATCTTGGTGTTTTGGGGACCAAGAGACTCCCGTACCCAAGTAGATCTTTTGAACTGGCACATTGTTCTCGCTGTAGGATCGATTGGCTTCAAAGGGATGGGATACTTCTCCTTGAGTTAG

Protein Analysis

299

Amino Acids

33.48

Weight (kDa)

8.43

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 484
Acc36I ACCTGC 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 718
AciI CCGC 1 cut(s) 167
AclI AACGTT 1 cut(s) 37
AclWI GGATC 6 cut(s) 19, 32, 327, 403, 738, 862
AcsI RAATTY 2 cut(s) 143, 767
AfaI GTAC 3 cut(s) 544, 572, 811
AfiI CCNNNNNNNGG 4 cut(s) 200, 398, 439, 539
AgeI ACCGGT 1 cut(s) 241
AgsI TTSAA 5 cut(s) 232, 313, 619, 829, 868
AjnI CCWGG 2 cut(s) 397, 532
AluBI AGCT 1 cut(s) 563
AluI AGCT 1 cut(s) 563
Alw26I GTCTC 5 cut(s) 83, 122, 340, 429, 795
AlwI GGATC 6 cut(s) 19, 32, 327, 403, 738, 862
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 1 cut(s) 422
ApoI RAATTY 2 cut(s) 143, 767
AsiGI ACCGGT 1 cut(s) 241
Asp700I GAANNNNTTC 1 cut(s) 270
AspS9I GGNCC 2 cut(s) 643, 794
AsuHPI GGTGA 5 cut(s) 37, 76, 180, 215, 527
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 2 cut(s) 643, 794
BaeI ACNNNNGTAYC 2 cut(s) 793, 826
BamHI GGATCC 1 cut(s) 24
BanI GGYRCC 1 cut(s) 718
BccI CCATC 4 cut(s) 212, 325, 496, 868
BciT130I CCWGG 2 cut(s) 399, 534
BciVI GTATCC 2 cut(s) 674, 872
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 5 cut(s) 83, 122, 340, 429, 795
BfaI CTAG 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 849
BfuAI ACCTGC 1 cut(s) 693
BfuI GTATCC 2 cut(s) 674, 872
BglI GCCNNNNNGGC 1 cut(s) 367
BglII AGATCT 1 cut(s) 820
Bme1390I CCNGG 2 cut(s) 399, 534
Bme18I GGWCC 2 cut(s) 643, 794
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 643, 794
BmiI GGNNCC 5 cut(s) 26, 109, 635, 720, 795
BmrFI CCNGG 2 cut(s) 399, 534
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 587
BmuI ACTGGG 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 618, 650
BpmI CTGGAG 1 cut(s) 555
Bsa29I ATCGAT 2 cut(s) 742, 857
BsaBI GATNNNNATC 1 cut(s) 732
BsaI GGTCTC 2 cut(s) 83, 122
BsaJI CCNNGG 1 cut(s) 714
BsaWI WCCGGW 2 cut(s) 113, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 398, 439, 539
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 3 cut(s) 356, 747, 836
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 399, 534
BseCI ATCGAT 2 cut(s) 742, 857
BseDI CCNNGG 1 cut(s) 714
BseGI GGATG 4 cut(s) 175, 223, 507, 879
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 4 cut(s) 200, 398, 439, 539
BseMII CTCAG 2 cut(s) 63, 471
BseNI ACTGG 3 cut(s) 356, 747, 836
BseRI GAGGAG 2 cut(s) 384, 552
BshFI GGCC 1 cut(s) 424
BshNI GGYRCC 1 cut(s) 718
BshTI ACCGGT 1 cut(s) 241
BshVI ATCGAT 2 cut(s) 742, 857
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 114, 242
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 4 cut(s) 200, 398, 439, 539
BsmAI GTCTC 5 cut(s) 83, 122, 340, 429, 795
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 807
BsnI GGCC 1 cut(s) 424
Bso31I GGTCTC 2 cut(s) 83, 122
BsoBI CYCGRG 1 cut(s) 119
Bsp143I GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 2 cut(s) 62, 472
BspDI ATCGAT 2 cut(s) 742, 857
BspLI GGNNCC 5 cut(s) 26, 109, 635, 720, 795
BspMI ACCTGC 1 cut(s) 693
BspPI GGATC 6 cut(s) 19, 32, 327, 403, 738, 862
BspQI GCTCTTC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 718
BspTNI GGTCTC 2 cut(s) 83, 122
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 3 cut(s) 356, 747, 836
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 714
BssMI GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 2 cut(s) 399, 534
Bst4CI ACNGT 2 cut(s) 380, 643
Bst6I CTCTTC 2 cut(s) 207, 227
BstDEI CTNAG 4 cut(s) 49, 96, 280, 480
BstEII GGTNACC 1 cut(s) 43
BstENI CCTNNNNNAGG 1 cut(s) 537
BstF5I GGATG 4 cut(s) 175, 223, 507, 879
BstKTI GATC 7 cut(s) 27, 69, 322, 411, 746, 823, 857
BstMAI GTCTC 5 cut(s) 83, 122, 340, 429, 795
BstMBI GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
BstMWI GCNNNNNNNGC 2 cut(s) 367, 584
BstNI CCWGG 2 cut(s) 399, 534
BstPI GGTNACC 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 397, 532
BstSFI CTRYAG 1 cut(s) 849
BstX2I RGATCY 4 cut(s) 24, 319, 408, 820
BstXI CCANNNNNNTGG 1 cut(s) 420
BstYI RGATCY 4 cut(s) 24, 319, 408, 820
Bsu15I ATCGAT 2 cut(s) 742, 857
BsuI GTATCC 2 cut(s) 674, 872
BsuRI GGCC 1 cut(s) 424
BsuTUI ATCGAT 2 cut(s) 742, 857
BtgZI GCGATG 1 cut(s) 559
BtsCI GGATG 4 cut(s) 175, 223, 507, 879
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 349, 363
BveI ACCTGC 1 cut(s) 693
CaiI CAGNNNCTG 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 2 cut(s) 643, 794
ClaI ATCGAT 2 cut(s) 742, 857
Csp6I GTAC 3 cut(s) 543, 571, 810
CspAI ACCGGT 1 cut(s) 241
CviJI RGCY 7 cut(s) 17, 424, 432, 563, 587, 634, 864
CviKI_1 RGCY 7 cut(s) 17, 424, 432, 563, 587, 634, 864
CviQI GTAC 3 cut(s) 543, 571, 810
DdeI CTNAG 4 cut(s) 49, 96, 280, 480
DpnI GATC 7 cut(s) 26, 68, 321, 410, 745, 822, 856
DpnII GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 207, 227
EarI CTCTTC 2 cut(s) 207, 227
Eco130I CCWWGG 1 cut(s) 714
Eco31I GGTCTC 2 cut(s) 83, 122
Eco47I GGWCC 2 cut(s) 643, 794
Eco88I CYCGRG 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 537
EcoO65I GGTNACC 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 767
EcoRII CCWGG 2 cut(s) 397, 532
EcoT14I CCWWGG 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 714
Esp3I CGTCTC 1 cut(s) 429
FalI AAGNNNNNCTT 2 cut(s) 807, 839
FaqI GGGAC 1 cut(s) 807
FbaI TGATCA 1 cut(s) 66
FokI GGATG 4 cut(s) 182, 230, 514, 886
FspBI CTAG 1 cut(s) 669
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 424
HapII CCGG 2 cut(s) 114, 242
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 4 cut(s) 185, 271, 385, 803
HpaII CCGG 2 cut(s) 114, 242
HphI GGTGA 5 cut(s) 37, 76, 180, 215, 527
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 265, 490, 638, 697
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 2 cut(s) 6, 624
HpyCH4III ACNGT 2 cut(s) 380, 643
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 1 cut(s) 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 367, 584
HpyF3I CTNAG 4 cut(s) 49, 96, 280, 480
HpySE526I ACGT 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 3 cut(s) 334, 560, 639
LweI GCATC 1 cut(s) 587
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 3 cut(s) 3, 43, 186
MalI GATC 7 cut(s) 26, 68, 321, 410, 745, 822, 856
MboI GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
MboII GAAGA 5 cut(s) 185, 218, 224, 244, 684
MflI RGATCY 4 cut(s) 24, 319, 408, 820
MluCI AATT 3 cut(s) 143, 414, 767
MlyI GAGTC 2 cut(s) 194, 797
MmeI TCCRAC 1 cut(s) 297
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 2 cut(s) 375, 417
MspI CCGG 2 cut(s) 114, 242
MspR9I CCNGG 2 cut(s) 399, 534
MvaI CCWGG 2 cut(s) 399, 534
MwoI GCNNNNNNNGC 2 cut(s) 367, 584
NdeII GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
NlaIV GGNNCC 5 cut(s) 26, 109, 635, 720, 795
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 3 cut(s) 3, 43, 186
PaeR7I CTCGAG 1 cut(s) 119
PciSI GCTCTTC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 271, 385
PfoI TCCNGGA 2 cut(s) 397, 532
PinAI ACCGGT 1 cut(s) 241
PleI GAGTC 2 cut(s) 193, 797
PpsI GAGTC 2 cut(s) 193, 797
Psp1406I AACGTT 1 cut(s) 37
Psp6I CCWGG 2 cut(s) 397, 532
PspEI GGTNACC 1 cut(s) 43
PspGI CCWGG 2 cut(s) 397, 532
PspN4I GGNNCC 5 cut(s) 26, 109, 635, 720, 795
PspPI GGNCC 2 cut(s) 643, 794
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 4 cut(s) 24, 319, 408, 820
RsaI GTAC 3 cut(s) 544, 572, 811
RsaNI GTAC 3 cut(s) 543, 571, 810
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 375, 417
Sau3AI GATC 7 cut(s) 24, 66, 319, 408, 743, 820, 854
Sau96I GGNCC 2 cut(s) 643, 794
SchI GAGTC 2 cut(s) 194, 797
ScrFI CCNGG 2 cut(s) 399, 534
SfaNI GCATC 1 cut(s) 587
SfcI CTRYAG 1 cut(s) 849
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 2 cut(s) 643, 794
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 119, 889
SmoI CTYRAG 2 cut(s) 119, 889
Sse9I AATT 3 cut(s) 143, 414, 767
SsiI CCGC 1 cut(s) 167
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 397, 532
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 2 cut(s) 380, 643
TaiI ACGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 120, 742, 857
TasI AATT 3 cut(s) 143, 414, 767
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 2 cut(s) 271, 385
Tru1I TTAA 2 cut(s) 375, 417
Tru9I TTAA 2 cut(s) 375, 417
TscAI CASTG 2 cut(s) 356, 363
TseFI GTSAC 3 cut(s) 3, 43, 186
Tsp45I GTSAC 3 cut(s) 3, 43, 186
TspDTI ATGAA 4 cut(s) 186, 225, 315, 722
TspRI CASTG 2 cut(s) 356, 363
VpaK11BI GGWCC 2 cut(s) 643, 794
XagI CCTNNNNNAGG 1 cut(s) 537
XapI RAATTY 2 cut(s) 143, 767
XhoI CTCGAG 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.