Methyltransferase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 192 |
| AccB1I | GGYRCC | 3 cut(s) | 217, 949, 1256 |
| AccBSI | CCGCTC | 1 cut(s) | 505 |
| AciI | CCGC | 6 cut(s) | 505, 560, 590, 1107, 1239, 1247 |
| AclWI | GGATC | 3 cut(s) | 238, 623, 1140 |
| AcsI | RAATTY | 2 cut(s) | 267, 474 |
| AcuI | CTGAAG | 1 cut(s) | 462 |
| AfaI | GTAC | 7 cut(s) | 43, 71, 405, 598, 677, 1011, 1309 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 38, 755, 959, 1183 |
| AgsI | TTSAA | 6 cut(s) | 329, 368, 788, 821, 919, 1091 |
| AjnI | CCWGG | 5 cut(s) | 31, 412, 592, 894, 1067 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 838, 870 |
| AluBI | AGCT | 3 cut(s) | 62, 803, 1320 |
| AluI | AGCT | 3 cut(s) | 62, 803, 1320 |
| Alw26I | GTCTC | 1 cut(s) | 295 |
| AlwI | GGATC | 3 cut(s) | 238, 623, 1140 |
| AoxI | GGCC | 2 cut(s) | 722, 1145 |
| ApeKI | GCWGC | 1 cut(s) | 524 |
| ApoI | RAATTY | 2 cut(s) | 267, 474 |
| AspS9I | GGNCC | 5 cut(s) | 294, 466, 962, 1071, 1146 |
| AsuHPI | GGTGA | 2 cut(s) | 26, 770 |
| AvaII | GGWCC | 4 cut(s) | 294, 466, 962, 1071 |
| BaeGI | GKGCMC | 1 cut(s) | 496 |
| BanI | GGYRCC | 3 cut(s) | 217, 949, 1256 |
| BanII | GRGCYC | 1 cut(s) | 845 |
| BbsI | GAAGAC | 2 cut(s) | 183, 963 |
| BbvI | GCAGC | 1 cut(s) | 511 |
| BccI | CCATC | 4 cut(s) | 368, 871, 1082, 1123 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 55, 89 |
| BciT130I | CCWGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| BciVI | GTATCC | 2 cut(s) | 173, 372 |
| BcoDI | GTCTC | 1 cut(s) | 295 |
| BfaI | CTAG | 1 cut(s) | 168 |
| BfmI | CTRYAG | 1 cut(s) | 349 |
| BfuAI | ACCTGC | 1 cut(s) | 192 |
| BfuI | GTATCC | 2 cut(s) | 173, 372 |
| BglII | AGATCT | 1 cut(s) | 320 |
| BisI | GCNGC | 3 cut(s) | 525, 560, 1108 |
| BlsI | GCNGC | 3 cut(s) | 526, 561, 1109 |
| Bme1390I | CCNGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| Bme18I | GGWCC | 4 cut(s) | 294, 466, 962, 1071 |
| BmgT120I | GGNCC | 5 cut(s) | 294, 466, 962, 1071, 1146 |
| BmiI | GGNNCC | 7 cut(s) | 134, 219, 295, 536, 717, 951, 1258 |
| BmrFI | CCNGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| BmrI | ACTGGG | 1 cut(s) | 1231 |
| BmsI | GCATC | 5 cut(s) | 86, 625, 675, 1146, 1234 |
| BmuI | ACTGGG | 1 cut(s) | 1231 |
| BpiI | GAAGAC | 2 cut(s) | 183, 963 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 117, 149 |
| BpmI | CTGGAG | 1 cut(s) | 54 |
| BpuEI | CTTGAG | 2 cut(s) | 410, 1162 |
| BsaBI | GATNNNNATC | 2 cut(s) | 231, 627 |
| BsaJI | CCNNGG | 3 cut(s) | 213, 719, 895 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 494, 524, 727, 757 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 38, 755, 959, 1183 |
| Bse1I | ACTGG | 4 cut(s) | 336, 833, 1022, 1226 |
| Bse3DI | GCAATG | 1 cut(s) | 213 |
| Bse8I | GATNNNNATC | 2 cut(s) | 231, 627 |
| BseBI | CCWGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| BseDI | CCNNGG | 3 cut(s) | 213, 719, 895 |
| BseGI | GGATG | 5 cut(s) | 379, 463, 513, 628, 1106 |
| BseJI | GATNNNNATC | 2 cut(s) | 231, 627 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 38, 755, 959, 1183 |
| BseMI | GCAATG | 1 cut(s) | 213 |
| BseMII | CTCAG | 2 cut(s) | 672, 1020 |
| BseNI | ACTGG | 4 cut(s) | 336, 833, 1022, 1226 |
| BseRI | GAGGAG | 1 cut(s) | 51 |
| BseSI | GKGCMC | 1 cut(s) | 496 |
| BseXI | GCAGC | 1 cut(s) | 511 |
| BshFI | GGCC | 2 cut(s) | 724, 1147 |
| BshNI | GGYRCC | 3 cut(s) | 217, 949, 1256 |
| BsiWI | CGTACG | 1 cut(s) | 1307 |
| BslFI | GGGAC | 1 cut(s) | 307 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 38, 755, 959, 1183 |
| BsmAI | GTCTC | 1 cut(s) | 295 |
| BsmFI | GGGAC | 1 cut(s) | 307 |
| BsnI | GGCC | 2 cut(s) | 724, 1147 |
| Bsp1286I | GDGCHC | 2 cut(s) | 496, 845 |
| Bsp143I | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| BspACI | CCGC | 6 cut(s) | 505, 560, 590, 1107, 1239, 1247 |
| BspANI | GGCC | 2 cut(s) | 724, 1147 |
| BspCNI | CTCAG | 2 cut(s) | 673, 1019 |
| BspLI | GGNNCC | 7 cut(s) | 134, 219, 295, 536, 717, 951, 1258 |
| BspMI | ACCTGC | 1 cut(s) | 192 |
| BspPI | GGATC | 3 cut(s) | 238, 623, 1140 |
| BspT107I | GGYRCC | 3 cut(s) | 217, 949, 1256 |
| BsrBI | CCGCTC | 1 cut(s) | 505 |
| BsrDI | GCAATG | 1 cut(s) | 213 |
| BsrI | ACTGG | 4 cut(s) | 336, 833, 1022, 1226 |
| BssECI | CCNNGG | 3 cut(s) | 213, 719, 895 |
| BssMI | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| BssT1I | CCWWGG | 2 cut(s) | 213, 719 |
| Bst2UI | CCWGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| Bst4CI | ACNGT | 3 cut(s) | 142, 544, 675 |
| Bst6I | CTCTTC | 2 cut(s) | 456, 808 |
| BstC8I | GCNNGC | 2 cut(s) | 64, 664 |
| BstDEI | CTNAG | 5 cut(s) | 105, 407, 470, 681, 1006 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 36 |
| BstF5I | GGATG | 5 cut(s) | 379, 463, 513, 628, 1106 |
| BstKTI | GATC | 6 cut(s) | 246, 323, 620, 631, 1057, 1135 |
| BstMAI | GTCTC | 1 cut(s) | 295 |
| BstMBI | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 83, 491, 774 |
| BstNI | CCWGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| BstSCI | CCNGG | 5 cut(s) | 31, 412, 592, 894, 1067 |
| BstSFI | CTRYAG | 1 cut(s) | 349 |
| BstSLI | GKGCMC | 1 cut(s) | 496 |
| BstV1I | GCAGC | 1 cut(s) | 511 |
| BstV2I | GAAGAC | 2 cut(s) | 183, 963 |
| BstX2I | RGATCY | 2 cut(s) | 243, 320 |
| BstYI | RGATCY | 2 cut(s) | 243, 320 |
| BsuI | GTATCC | 2 cut(s) | 173, 372 |
| BsuRI | GGCC | 2 cut(s) | 724, 1147 |
| BtgZI | GCGATG | 2 cut(s) | 58, 500 |
| BtsCI | GGATG | 5 cut(s) | 379, 463, 513, 628, 1106 |
| BtsI | GCAGTG | 1 cut(s) | 1236 |
| BtsIMutI | CAGTG | 2 cut(s) | 1219, 1236 |
| BveI | ACCTGC | 1 cut(s) | 192 |
| Cac8I | GCNNGC | 2 cut(s) | 64, 664 |
| Cfr13I | GGNCC | 5 cut(s) | 294, 466, 962, 1071, 1146 |
| Csp6I | GTAC | 7 cut(s) | 42, 70, 404, 597, 676, 1010, 1308 |
| CviAII | CATG | 4 cut(s) | 580, 796, 883, 1064 |
| CviQI | GTAC | 7 cut(s) | 42, 70, 404, 597, 676, 1010, 1308 |
| DdeI | CTNAG | 5 cut(s) | 105, 407, 470, 681, 1006 |
| DpnI | GATC | 6 cut(s) | 245, 322, 619, 630, 1056, 1134 |
| DpnII | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| DraI | TTTAAA | 1 cut(s) | 117 |
| Eam1104I | CTCTTC | 2 cut(s) | 456, 808 |
| EarI | CTCTTC | 2 cut(s) | 456, 808 |
| Eco130I | CCWWGG | 2 cut(s) | 213, 719 |
| Eco24I | GRGCYC | 1 cut(s) | 845 |
| Eco47I | GGWCC | 4 cut(s) | 294, 466, 962, 1071 |
| Eco57I | CTGAAG | 1 cut(s) | 462 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 36 |
| EcoO109I | RGGNCCY | 1 cut(s) | 466 |
| EcoRI | GAATTC | 1 cut(s) | 267 |
| EcoRII | CCWGG | 5 cut(s) | 31, 412, 592, 894, 1067 |
| EcoT14I | CCWWGG | 2 cut(s) | 213, 719 |
| EcoT38I | GRGCYC | 1 cut(s) | 845 |
| ErhI | CCWWGG | 2 cut(s) | 213, 719 |
| FaeI | CATG | 4 cut(s) | 583, 799, 886, 1067 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 307, 339, 453, 485 |
| FaqI | GGGAC | 1 cut(s) | 307 |
| FatI | CATG | 4 cut(s) | 579, 795, 882, 1063 |
| FauNDI | CATATG | 1 cut(s) | 1206 |
| Fnu4HI | GCNGC | 3 cut(s) | 525, 560, 1108 |
| FokI | GGATG | 5 cut(s) | 386, 470, 520, 635, 1113 |
| FriOI | GRGCYC | 1 cut(s) | 845 |
| Fsp4HI | GCNGC | 3 cut(s) | 525, 560, 1108 |
| FspBI | CTAG | 1 cut(s) | 168 |
| GluI | GCNGC | 3 cut(s) | 525, 560, 1108 |
| GsuI | CTGGAG | 1 cut(s) | 54 |
| HaeIII | GGCC | 2 cut(s) | 724, 1147 |
| Hin1II | CATG | 4 cut(s) | 583, 799, 886, 1067 |
| HincII | GTYRAC | 1 cut(s) | 1114 |
| HindII | GTYRAC | 1 cut(s) | 1114 |
| HindIII | AAGCTT | 1 cut(s) | 801 |
| HinfI | GANTC | 2 cut(s) | 303, 832 |
| HphI | GGTGA | 2 cut(s) | 26, 770 |
| Hpy166II | GTNNAC | 2 cut(s) | 1012, 1114 |
| Hpy188I | TCNGA | 7 cut(s) | 137, 196, 243, 622, 682, 859, 1084 |
| Hpy188III | TCNNGA | 4 cut(s) | 440, 632, 1141, 1291 |
| Hpy8I | GTNNAC | 2 cut(s) | 1012, 1114 |
| HpyAV | CCTTC | 3 cut(s) | 123, 880, 1204 |
| HpyCH4III | ACNGT | 3 cut(s) | 142, 544, 675 |
| HpyCH4V | TGCA | 7 cut(s) | 206, 254, 275, 615, 638, 666, 908 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 83, 491, 774 |
| HpyF3I | CTNAG | 5 cut(s) | 105, 407, 470, 681, 1006 |
| Hsp92II | CATG | 4 cut(s) | 583, 799, 886, 1067 |
| Kzo9I | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| LmnI | GCTCC | 4 cut(s) | 59, 138, 502, 721 |
| Lsp1109I | GCAGC | 1 cut(s) | 511 |
| LweI | GCATC | 5 cut(s) | 86, 625, 675, 1146, 1234 |
| MaeI | CTAG | 1 cut(s) | 168 |
| MaeIII | GTNAC | 2 cut(s) | 575, 1215 |
| MalI | GATC | 6 cut(s) | 245, 322, 619, 630, 1056, 1134 |
| MbiI | CCGCTC | 1 cut(s) | 505 |
| MboI | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| MboII | GAAGA | 6 cut(s) | 183, 473, 825, 931, 968, 1069 |
| MflI | RGATCY | 2 cut(s) | 243, 320 |
| MhlI | GDGCHC | 2 cut(s) | 496, 845 |
| MluCI | AATT | 6 cut(s) | 248, 267, 474, 519, 823, 1122 |
| MlyI | GAGTC | 1 cut(s) | 297 |
| MmeI | TCCRAC | 2 cut(s) | 882, 1107 |
| MseI | TTAA | 3 cut(s) | 116, 975, 1121 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1163 |
| MspA1I | CMGCKG | 2 cut(s) | 562, 1241 |
| MspR9I | CCNGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| MvaI | CCWGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 83, 491, 774 |
| NdeI | CATATG | 1 cut(s) | 1206 |
| NdeII | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| NlaIII | CATG | 4 cut(s) | 583, 799, 886, 1067 |
| NlaIV | GGNNCC | 7 cut(s) | 134, 219, 295, 536, 717, 951, 1258 |
| PfeI | GAWTC | 1 cut(s) | 832 |
| Pfl23II | CGTACG | 1 cut(s) | 1307 |
| PfoI | TCCNGGA | 1 cut(s) | 31 |
| PkrI | GCNGC | 3 cut(s) | 526, 561, 1109 |
| PleI | GAGTC | 1 cut(s) | 297 |
| PpsI | GAGTC | 1 cut(s) | 297 |
| PpuMI | RGGWCCY | 1 cut(s) | 466 |
| Psp5II | RGGWCCY | 1 cut(s) | 466 |
| Psp6I | CCWGG | 5 cut(s) | 31, 412, 592, 894, 1067 |
| PspGI | CCWGG | 5 cut(s) | 31, 412, 592, 894, 1067 |
| PspLI | CGTACG | 1 cut(s) | 1307 |
| PspN4I | GGNNCC | 7 cut(s) | 134, 219, 295, 536, 717, 951, 1258 |
| PspPI | GGNCC | 5 cut(s) | 294, 466, 962, 1071, 1146 |
| PspPPI | RGGWCCY | 1 cut(s) | 466 |
| PsuI | RGATCY | 2 cut(s) | 243, 320 |
| RsaI | GTAC | 7 cut(s) | 43, 71, 405, 598, 677, 1011, 1309 |
| RsaNI | GTAC | 7 cut(s) | 42, 70, 404, 597, 676, 1010, 1308 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1163 |
| SaqAI | TTAA | 3 cut(s) | 116, 975, 1121 |
| SatI | GCNGC | 3 cut(s) | 525, 560, 1108 |
| Sau3AI | GATC | 6 cut(s) | 243, 320, 617, 628, 1054, 1132 |
| Sau96I | GGNCC | 5 cut(s) | 294, 466, 962, 1071, 1146 |
| SchI | GAGTC | 1 cut(s) | 297 |
| ScrFI | CCNGG | 5 cut(s) | 33, 414, 594, 896, 1069 |
| SduI | GDGCHC | 2 cut(s) | 496, 845 |
| SfaNI | GCATC | 5 cut(s) | 86, 625, 675, 1146, 1234 |
| SfcI | CTRYAG | 1 cut(s) | 349 |
| SinI | GGWCC | 4 cut(s) | 294, 466, 962, 1071 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1163 |
| SmlI | CTYRAG | 2 cut(s) | 389, 1141 |
| SmoI | CTYRAG | 2 cut(s) | 389, 1141 |
| Sse9I | AATT | 6 cut(s) | 248, 267, 474, 519, 823, 1122 |
| SsiI | CCGC | 6 cut(s) | 505, 560, 590, 1107, 1239, 1247 |
| SspMI | CTAG | 1 cut(s) | 168 |
| StyD4I | CCNGG | 5 cut(s) | 31, 412, 592, 894, 1067 |
| StyI | CCWWGG | 2 cut(s) | 213, 719 |
| TaaI | ACNGT | 3 cut(s) | 142, 544, 675 |
| TaqI | TCGA | 1 cut(s) | 730 |
| TasI | AATT | 6 cut(s) | 248, 267, 474, 519, 823, 1122 |
| TatI | WGTACW | 2 cut(s) | 69, 1009 |
| TauI | GCSGC | 2 cut(s) | 562, 1110 |
| TfiI | GAWTC | 1 cut(s) | 832 |
| Tru1I | TTAA | 3 cut(s) | 116, 975, 1121 |
| Tru9I | TTAA | 3 cut(s) | 116, 975, 1121 |
| TscAI | CASTG | 2 cut(s) | 1226, 1236 |
| TseI | GCWGC | 1 cut(s) | 524 |
| TspDTI | ATGAA | 9 cut(s) | 221, 474, 891, 899, 956, 1052, 1147, 1183, 1223 |
| TspGWI | ACGGA | 1 cut(s) | 975 |
| TspRI | CASTG | 2 cut(s) | 1226, 1236 |
| VpaK11BI | GGWCC | 4 cut(s) | 294, 466, 962, 1071 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 36 |
| XapI | RAATTY | 2 cut(s) | 267, 474 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 59 |
| XspI | CTAG | 1 cut(s) | 168 |