Rroxscaffold_7G00180820

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000529
Physical Location & Seq
Reverse (-)
20137115 .. 20141735
4621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180820.1

Sequence Viewer

Length: 1749 bp
ATGTCGCGGGGACGAGCTGATGGGGGCTCAAAGAAACGTTTGGTCACCTCAGTTCTAATTTTGGTGATTATTTGTGGTGGGTTGTACATGTATACTAAAAGAAATGGTTCCACCGCTTTGGAGTATAGCAGTAAAATAAGAAAATTCGGTTCTACTTACTTGGGTGGGGATGAAGAGGTTGACGAGACACCTCCCAAACTTGGTGAAGATGAAGATGATGGTGTTATACTGAAGAGCATACCAGTTTGTGATGATAGACATTCAGAATTAATTCCTTGCTTAGACAGAAACCTTATTTACCAGACAAGGTTGAAGTTGGATCTGTCTTTGATGGAACATTATGAGAGACACTGCCCTGCGTCTGAAAAGCGATTTAACTGTTTAATTCCACCCCCACCTGGATACAAGATCCCCATCAAGTGGCCTAAAAGCAGAGATGAGATTGGATTTCCTGGAGGAGGTACTCACTTTCATTATGGAGCTGATAAGTACATCGCAGCAATGGCTAATATGCTCAACTTTCCTAAGAATAATTTAAACAATGAGGGAAATCTTCGGACAGTCCTTGATGTTGGCTGTGGTGTTGCTAGTTTTGGAGGTTACCTGCTGTCTTCAGATATCCTTACAATGTCCTTGGCACCTAATGATGTCCATCAAAATCAGATCCAGTTTGCTTTAGAAAGAGGAATTCCTGCATATCTTGGTGTTCTAGGGACAAAGAGACTCCCTTACCCAAGTCGATCTTTTGAACTGGCACATTGTTCTCGCTGTAGGATTGACTGGCTTCAAAGGGATGGGATACTTCTCCTTGAGCTAGATAGGGTACTCAGACCAGGAGGCTATTTTGCCTACTCATCTCCCGAAGCCTATGCACAGGATGAGGAGGATCTGAGAATATGGAAGCAGATGAGTGCCCTTGTGGAACGGATGTGTTGGAAAATAGCTGCTAAAAAGAACCAAACTGTTATTTGGGAAAAGCCCATGACTAATGATTGTTACATGGCAAGAGAGCCTGGAACTCAACCTCCGCTTTGTAAATCGGGTGCTGATCCAGATGCCACCTGGGATGTGAAAATGGAGGCTTGCATCACTCCATACACTGAACAAATTCAGAGAGCAAGGGGAAGTGGTTTGGCTCCTTGGCCTGCTCGATTGACAACACCTCCTCCCCGTCTTACTGACTTTGGCTATTCTGCTGATGTATTTGAAAAGGACATGGAAGTATGGCAGCAAAGAGTTGACAATTACTGGAATCTTTTGAGCCCAAAGATTAATCCCGACACAGTGAGAAATGTGATGGACATGAAGGCACACATGGGTTCATTTGCAGCTTCTTTAAGGAGCAAACCTGTTTGGGTTATGAATGTGGTGCCAGAAGATGGACCTAACACACTGAAAATAATATATGATAGAGGCCTAATAGGCTCAATACACAGCTGGTGTGAAGCCTACTCAACCTACCCGAGAACTTATGATCTACTTCATGCTTGGACTGTCTTCTCTGATGTTGAAAGGAAAGGATGTAGTGCCGTTGACTTGATGATTGAGATGGATCGCATCCTCCGGCCCAAAGGTTTCATCATTGTCCGTGATAAGCCGAAGGTAGTGGAGTACATTAAAAAATACATGAAGGCATTACACTGGGAAGCAGTGGCTAGTGCTGATGCAGACCAAGATGAGGATGATGCAGTGTTGATTATCCAGAAAAAGATTTGGCGTACGAGCGAGAGCGTTAGGGAGTTAGAATAG

Protein Analysis

582

Amino Acids

66.02

Weight (kDa)

6.46

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 89 - 148 2.6e-18 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_29 PF03141 147 - 569 3.4e-189 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 189 - 283 4.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 2 cut(s) 637, 1369
AccB7I CCANNNNNTGG 2 cut(s) 420, 1379
AccI GTMKAC 1 cut(s) 92
AccII CGCG 1 cut(s) 7
AciI CCGC 3 cut(s) 7, 114, 1028
AclI AACGTT 1 cut(s) 37
AclWI GGATC 6 cut(s) 327, 403, 658, 894, 1043, 1560
AcsI RAATTY 3 cut(s) 143, 687, 1107
AcuI CTGAAG 2 cut(s) 251, 597
AfaI GTAC 6 cut(s) 86, 463, 491, 825, 1613, 1720
AfiI CCNNNNNNNGG 6 cut(s) 200, 398, 420, 458, 1379, 1678
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 5 cut(s) 313, 749, 788, 1208, 1511
AjnI CCWGG 5 cut(s) 397, 451, 832, 1012, 1061
AloI GAACNNNNNNTCC 2 cut(s) 1009, 1041
AluBI AGCT 6 cut(s) 17, 482, 814, 944, 1331, 1437
AluI AGCT 6 cut(s) 17, 482, 814, 944, 1331, 1437
Alw26I GTCTC 3 cut(s) 179, 340, 715
AlwI GGATC 6 cut(s) 327, 403, 658, 894, 1043, 1560
Ama87I CYCGRG 1 cut(s) 1462
AoxI GGCC 4 cut(s) 422, 1142, 1414, 1565
ApeKI GCWGC 4 cut(s) 497, 944, 1228, 1328
ApoI RAATTY 3 cut(s) 143, 687, 1107
AseI ATTAAT 2 cut(s) 269, 1272
Asp700I GAANNNNTTC 3 cut(s) 106, 270, 1107
AspS9I GGNCC 2 cut(s) 1382, 1566
AsuHPI GGTGA 3 cut(s) 37, 76, 215
AvaI CYCGRG 1 cut(s) 1462
AvaII GGWCC 1 cut(s) 1382
BaeGI GKGCMC 1 cut(s) 916
BanI GGYRCC 2 cut(s) 637, 1369
BanII GRGCYC 2 cut(s) 29, 1265
BbsI GAAGAC 2 cut(s) 603, 1489
BbvI GCAGC 4 cut(s) 509, 931, 1240, 1340
BccI CCATC 9 cut(s) 14, 212, 325, 422, 660, 788, 1291, 1373, 1543
BceAI ACGGC 1 cut(s) 1514
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 5 cut(s) 399, 453, 834, 1014, 1063
BciVI GTATCC 2 cut(s) 395, 792
BcoDI GTCTC 3 cut(s) 179, 340, 715
BfaI CTAG 4 cut(s) 588, 710, 815, 1656
BfmI CTRYAG 1 cut(s) 769
BfuAI ACCTGC 1 cut(s) 612
BfuI GTATCC 2 cut(s) 395, 792
BglI GCCNNNNNGGC 1 cut(s) 1422
BisI GCNGC 4 cut(s) 498, 945, 1229, 1329
BlsI GCNGC 4 cut(s) 499, 946, 1230, 1330
Bme1390I CCNGG 5 cut(s) 399, 453, 834, 1014, 1063
Bme18I GGWCC 1 cut(s) 1382
BmeT110I CYCGRG 1 cut(s) 1462
BmgT120I GGNCC 2 cut(s) 1382, 1566
BmiI GGNNCC 4 cut(s) 109, 639, 1137, 1371
BmrFI CCNGG 5 cut(s) 399, 453, 834, 1014, 1063
BmrI ACTGGG 1 cut(s) 1651
BmsI GCATC 5 cut(s) 1045, 1095, 1566, 1654, 1675
BmuI ACTGGG 1 cut(s) 1651
BpiI GAAGAC 2 cut(s) 603, 1489
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 830
BsaBI GATNNNNATC 2 cut(s) 413, 651
BsaJI CCNNGG 3 cut(s) 633, 1062, 1139
BsaXI ACNNNNNCTCC 6 cut(s) 1009, 1039, 1147, 1150, 1177, 1180
Bsc4I CCNNNNNNNGG 6 cut(s) 200, 398, 420, 458, 1379, 1678
Bse1I ACTGG 6 cut(s) 242, 667, 756, 785, 1253, 1646
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 2 cut(s) 413, 651
BseBI CCWGG 5 cut(s) 399, 453, 834, 1014, 1063
BseDI CCNNGG 3 cut(s) 633, 1062, 1139
BseGI GGATG 8 cut(s) 175, 799, 883, 933, 1072, 1526, 1557, 1687
BseJI GATNNNNATC 2 cut(s) 413, 651
BseLI CCNNNNNNNGG 6 cut(s) 200, 398, 420, 458, 1379, 1678
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 3 cut(s) 63, 841, 881
BseNI ACTGG 6 cut(s) 242, 667, 756, 785, 1253, 1646
BseRI GAGGAG 3 cut(s) 471, 896, 1155
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 4 cut(s) 509, 931, 1240, 1340
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 4 cut(s) 424, 1144, 1416, 1567
BshNI GGYRCC 2 cut(s) 637, 1369
BsiHKCI CYCGRG 1 cut(s) 1462
BsiSI CCGG 1 cut(s) 1564
BsiWI CGTACG 1 cut(s) 1718
BslFI GGGAC 2 cut(s) 24, 727
BslI CCNNNNNNNGG 6 cut(s) 200, 398, 420, 458, 1379, 1678
BsmAI GTCTC 3 cut(s) 179, 340, 715
BsmFI GGGAC 2 cut(s) 24, 727
BsnI GGCC 4 cut(s) 424, 1144, 1416, 1567
BsoBI CYCGRG 1 cut(s) 1462
Bsp1286I GDGCHC 3 cut(s) 29, 916, 1265
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
BspACI CCGC 3 cut(s) 7, 114, 1028
BspANI GGCC 4 cut(s) 424, 1144, 1416, 1567
BspCNI CTCAG 3 cut(s) 62, 840, 882
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 109, 639, 1137, 1371
BspMI ACCTGC 1 cut(s) 612
BspPI GGATC 6 cut(s) 327, 403, 658, 894, 1043, 1560
BspQI GCTCTTC 1 cut(s) 227
BspT107I GGYRCC 2 cut(s) 637, 1369
BsrDI GCAATG 1 cut(s) 507
BsrGI TGTACA 1 cut(s) 84
BsrI ACTGG 6 cut(s) 242, 667, 756, 785, 1253, 1646
BssECI CCNNGG 3 cut(s) 633, 1062, 1139
BssMI GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
BssNAI GTATAC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 633, 1139
Bst1107I GTATAC 1 cut(s) 93
Bst2UI CCWGG 5 cut(s) 399, 453, 834, 1014, 1063
Bst4CI ACNGT 5 cut(s) 380, 562, 964, 1285, 1495
Bst6I CTCTTC 2 cut(s) 168, 227
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 1084, 1146
BstDEI CTNAG 5 cut(s) 49, 280, 525, 827, 890
BstEII GGTNACC 2 cut(s) 43, 599
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 8 cut(s) 175, 799, 883, 933, 1072, 1526, 1557, 1687
BstFNI CGCG 1 cut(s) 7
BstKTI GATC 8 cut(s) 322, 411, 666, 743, 889, 1051, 1477, 1555
BstMAI GTCTC 3 cut(s) 179, 340, 715
BstMBI GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
BstMWI GCNNNNNNNGC 2 cut(s) 503, 1422
BstNI CCWGG 5 cut(s) 399, 453, 834, 1014, 1063
BstNSI RCATGY 1 cut(s) 91
BstPI GGTNACC 2 cut(s) 43, 599
BstSCI CCNGG 5 cut(s) 397, 451, 832, 1012, 1061
BstSFI CTRYAG 1 cut(s) 769
BstSLI GKGCMC 1 cut(s) 916
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 4 cut(s) 509, 931, 1240, 1340
BstV2I GAAGAC 2 cut(s) 603, 1489
BstX2I RGATCY 4 cut(s) 319, 408, 663, 886
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 4 cut(s) 319, 408, 663, 886
BstZ17I GTATAC 1 cut(s) 93
BsuI GTATCC 2 cut(s) 395, 792
BsuRI GGCC 4 cut(s) 424, 1144, 1416, 1567
BtgZI GCGATG 1 cut(s) 478
BtsCI GGATG 8 cut(s) 175, 799, 883, 933, 1072, 1526, 1557, 1687
BtsI GCAGTG 3 cut(s) 349, 1656, 1695
BtsIMutI CAGTG 7 cut(s) 349, 1098, 1290, 1391, 1639, 1656, 1695
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 2 cut(s) 1084, 1146
Cfr13I GGNCC 2 cut(s) 1382, 1566
CseI GACGC 1 cut(s) 348
Csp6I GTAC 6 cut(s) 85, 462, 490, 824, 1612, 1719
CviAII CATG 8 cut(s) 88, 982, 1000, 1216, 1303, 1315, 1484, 1627
CviQI GTAC 6 cut(s) 85, 462, 490, 824, 1612, 1719
DdeI CTNAG 5 cut(s) 49, 280, 525, 827, 890
DpnI GATC 8 cut(s) 321, 410, 665, 742, 888, 1050, 1476, 1554
DpnII GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
DraI TTTAAA 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 168, 227
EarI CTCTTC 2 cut(s) 168, 227
Eco130I CCWWGG 2 cut(s) 633, 1139
Eco147I AGGCCT 1 cut(s) 1416
Eco24I GRGCYC 2 cut(s) 29, 1265
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 1382
Eco57I CTGAAG 2 cut(s) 251, 597
Eco88I CYCGRG 1 cut(s) 1462
Eco91I GGTNACC 2 cut(s) 43, 599
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO65I GGTNACC 2 cut(s) 43, 599
EcoRI GAATTC 1 cut(s) 687
EcoRII CCWGG 5 cut(s) 397, 451, 832, 1012, 1061
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 2 cut(s) 633, 1139
EcoT38I GRGCYC 2 cut(s) 29, 1265
ErhI CCWWGG 2 cut(s) 633, 1139
FaeI CATG 8 cut(s) 91, 985, 1003, 1219, 1306, 1318, 1487, 1630
FalI AAGNNNNNCTT 2 cut(s) 727, 759
FaqI GGGAC 2 cut(s) 24, 727
FatI CATG 8 cut(s) 87, 981, 999, 1215, 1302, 1314, 1483, 1626
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 4 cut(s) 498, 945, 1229, 1329
FokI GGATG 8 cut(s) 182, 806, 890, 940, 1079, 1533, 1544, 1694
FriOI GRGCYC 2 cut(s) 29, 1265
Fsp4HI GCNGC 4 cut(s) 498, 945, 1229, 1329
FspBI CTAG 4 cut(s) 588, 710, 815, 1656
GluI GCNGC 4 cut(s) 498, 945, 1229, 1329
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 4 cut(s) 424, 1144, 1416, 1567
HapII CCGG 1 cut(s) 1564
HgaI GACGC 1 cut(s) 348
Hin1II CATG 8 cut(s) 91, 985, 1003, 1219, 1306, 1318, 1487, 1630
HincII GTYRAC 3 cut(s) 181, 1240, 1534
HindII GTYRAC 3 cut(s) 181, 1240, 1534
HinfI GANTC 2 cut(s) 723, 1252
HpaII CCGG 1 cut(s) 1564
HphI GGTGA 3 cut(s) 37, 76, 215
Hpy166II GTNNAC 4 cut(s) 93, 181, 1240, 1534
Hpy188I TCNGA 9 cut(s) 265, 364, 558, 616, 663, 830, 891, 1113, 1504
Hpy188III TCNNGA 4 cut(s) 860, 1052, 1277, 1702
Hpy8I GTNNAC 4 cut(s) 93, 181, 1240, 1534
HpyAV CCTTC 3 cut(s) 1300, 1594, 1624
HpyCH4III ACNGT 5 cut(s) 380, 562, 964, 1285, 1495
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 6 cut(s) 695, 872, 1086, 1328, 1667, 1688
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 1422
HpyF3I CTNAG 5 cut(s) 49, 280, 525, 827, 890
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 8 cut(s) 91, 985, 1003, 1219, 1306, 1318, 1487, 1630
Kzo9I GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 3 cut(s) 479, 1141, 1341
Lsp1109I GCAGC 4 cut(s) 509, 931, 1240, 1340
LweI GCATC 5 cut(s) 1045, 1095, 1566, 1654, 1675
MaeI CTAG 4 cut(s) 588, 710, 815, 1656
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 3 cut(s) 43, 599, 995
MalI GATC 8 cut(s) 321, 410, 665, 742, 888, 1050, 1476, 1554
MboI GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
MboII GAAGA 8 cut(s) 185, 218, 224, 244, 545, 603, 1388, 1489
MflI RGATCY 4 cut(s) 319, 408, 663, 886
MhlI GDGCHC 3 cut(s) 29, 916, 1265
MluCI AATT 9 cut(s) 57, 143, 266, 270, 384, 532, 687, 1107, 1243
MlyI GAGTC 1 cut(s) 717
MmeI TCCRAC 2 cut(s) 297, 914
MroXI GAANNNNTTC 3 cut(s) 106, 270, 1107
MseI TTAA 7 cut(s) 269, 375, 383, 536, 1272, 1337, 1617
MspA1I CMGCKG 1 cut(s) 1437
MspI CCGG 1 cut(s) 1564
MspR9I CCNGG 5 cut(s) 399, 453, 834, 1014, 1063
MvaI CCWGG 5 cut(s) 399, 453, 834, 1014, 1063
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 503, 1422
NdeII GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
NlaIII CATG 8 cut(s) 91, 985, 1003, 1219, 1306, 1318, 1487, 1630
NlaIV GGNNCC 4 cut(s) 109, 639, 1137, 1371
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 91
PceI AGGCCT 1 cut(s) 1416
PciI ACATGT 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 227
PdmI GAANNNNTTC 3 cut(s) 106, 270, 1107
PfeI GAWTC 1 cut(s) 1252
Pfl23II CGTACG 1 cut(s) 1718
PflMI CCANNNNNTGG 2 cut(s) 420, 1379
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 4 cut(s) 499, 946, 1230, 1330
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
PscI ACATGT 1 cut(s) 87
PshBI ATTAAT 2 cut(s) 269, 1272
Psp1406I AACGTT 1 cut(s) 37
Psp6I CCWGG 5 cut(s) 397, 451, 832, 1012, 1061
PspEI GGTNACC 2 cut(s) 43, 599
PspGI CCWGG 5 cut(s) 397, 451, 832, 1012, 1061
PspLI CGTACG 1 cut(s) 1718
PspN4I GGNNCC 4 cut(s) 109, 639, 1137, 1371
PspPI GGNCC 2 cut(s) 1382, 1566
PsuI RGATCY 4 cut(s) 319, 408, 663, 886
PvuII CAGCTG 1 cut(s) 1437
RsaI GTAC 6 cut(s) 86, 463, 491, 825, 1613, 1720
RsaNI GTAC 6 cut(s) 85, 462, 490, 824, 1612, 1719
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 7 cut(s) 269, 375, 383, 536, 1272, 1337, 1617
SatI GCNGC 4 cut(s) 498, 945, 1229, 1329
Sau3AI GATC 8 cut(s) 319, 408, 663, 740, 886, 1048, 1474, 1552
Sau96I GGNCC 2 cut(s) 1382, 1566
SchI GAGTC 1 cut(s) 717
ScrFI CCNGG 5 cut(s) 399, 453, 834, 1014, 1063
SduI GDGCHC 3 cut(s) 29, 916, 1265
SfaNI GCATC 5 cut(s) 1045, 1095, 1566, 1654, 1675
SfcI CTRYAG 1 cut(s) 769
SinI GGWCC 1 cut(s) 1382
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
Sse9I AATT 9 cut(s) 57, 143, 266, 270, 384, 532, 687, 1107, 1243
SseBI AGGCCT 1 cut(s) 1416
SsiI CCGC 3 cut(s) 7, 114, 1028
SspMI CTAG 4 cut(s) 588, 710, 815, 1656
StuI AGGCCT 1 cut(s) 1416
StyD4I CCNGG 5 cut(s) 397, 451, 832, 1012, 1061
StyI CCWWGG 2 cut(s) 633, 1139
TaaI ACNGT 5 cut(s) 380, 562, 964, 1285, 1495
TaiI ACGT 1 cut(s) 40
TaqI TCGA 2 cut(s) 739, 1150
TasI AATT 9 cut(s) 57, 143, 266, 270, 384, 532, 687, 1107, 1243
TatI WGTACW 3 cut(s) 84, 489, 1611
TfiI GAWTC 1 cut(s) 1252
Tru1I TTAA 7 cut(s) 269, 375, 383, 536, 1272, 1337, 1617
Tru9I TTAA 7 cut(s) 269, 375, 383, 536, 1272, 1337, 1617
TscAI CASTG 7 cut(s) 356, 1105, 1290, 1398, 1646, 1656, 1695
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 4 cut(s) 497, 944, 1228, 1328
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 9 cut(s) 186, 225, 461, 1311, 1319, 1376, 1472, 1567, 1643
TspGWI ACGGA 2 cut(s) 940, 1577
TspRI CASTG 7 cut(s) 356, 1105, 1290, 1398, 1646, 1656, 1695
Van91I CCANNNNNTGG 2 cut(s) 420, 1379
VpaK11BI GGWCC 1 cut(s) 1382
VspI ATTAAT 2 cut(s) 269, 1272
XagI CCTNNNNNAGG 1 cut(s) 456
XapI RAATTY 3 cut(s) 143, 687, 1107
XceI RCATGY 1 cut(s) 91
XcmI CCANNNNNNNNNTGG 1 cut(s) 1059
XmiI GTMKAC 1 cut(s) 92
XmnI GAANNNNTTC 3 cut(s) 106, 270, 1107
XspI CTAG 4 cut(s) 588, 710, 815, 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.