Rorug03G0372400

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000529
Physical Location & Seq
Forward (+)
45724968 .. 45730171
5204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0372400.1

Sequence Viewer

Length: 1530 bp
ATGCAACAGCAAATTGACGAACTGAAAGTACTAGTGAAGGAAGAAGTTTTCGCATCATATGATGTTTCATATCAGTTGAAATTAATTGATGAAATCCAACGCCTAGGTGTGGCATACCATTTTGAAACAGAAATAGATGAAGCACTAGAAAATATCTATGTTAAATACCATGATGATGGTGATCTGGACCTATACAATGTTTCTCTTCGTTTTCGTCTACTAAGACAAAATGGATATAATGTTTCATCTGATATATTCAGCAAATTTAAAGATGTAAACAGTTACTTCAAGGAGAGCTTAATTGCTGATATCTTAGCTATCCTATGCTTGTATGAGGCAACACATCTTAGGGTTCATGGAGAAGAAATACTAGAAGAAGCTCTTGTTTTCACCACCACCCACCTTGAATTGGCGATAAGTGGTGTAAGCTATCCACTAGCTGCAAAAATATCTCAAGCCCTAGAGAGGCCTCTGCGTAAAGGTCTAGAGAGGTTATGTACCAGGAATTACATACCAATCTACGAAGCCATGACTCCGCATAACGAAGCGCTACTGAAACTTGCGAAGTTAGATTTCAATCTGGTTCAATCTTTGCACAAAGAGGAGCTTAGTGAGGTTTCAAGGTGGGGGAAAGAACTAGGCTTTGAAAAGAATCTTCCTTTTGTAAGGTATAGGATTGTGGAGTGTTTCTTTTGGATGGTGGGAATGTATTTTGAACCCCGATACTCGGTTGGAAGAATAATCGGAACAAAACTGTGTGTTCTAGCTACAATTTTGGATGATATCTTTGATGCATGTGGTACACTTGAAGAACTCAAGATATTTCGTGAAGCAGTTGACAGGTTTGATGTCAACTATTGCACAAATGGTCTACCACGGTACATGCAAGTATACTATCATTTACTTTTGAATACTATGAATGAAATTGAGGAAGAGCTAGAAAAGCAAGGAATATCGTACCGAGTCCACTACGCAAAACAAGCTTTGAAGAATCAAGCTAGAGACTACCTTATTGAGGCCCAATGGCTCCATGAAGGATGTATCCCTTGCATGGAGGAGTATATGCATGTCAGATTGACTTCTGTTGGTGTTGCTATGACTGTAGTCTTTTCTTTAATTGGAATGGAAGAAACTATTACCGAGGAAACATTTGAGTGGATTTTGAAGTACCCGAAAAGTATTAGGGCTTCGAGCCTTATCTTTAGGCTCATGGATGACATTGCGGGGAGTAAGCATAAGAAAGAAAAAGGGGATGTTGCTTCTAGTATTGAATGTTACATGAAGCAATATGAGGTCTCAGAGAAAGAGACTATCGATGTGTTTAACAAACAAATTATAGATGCATGGAAGGACATAAATGAGGATTTACTTCGACCAACTGTTGTGCCAATGTGTGCGATTAAGCTCGGTCTTAATTTCGCAAGAGGTGTTGATCTGGTTTACAAAGAAGAAGATGAATTCACATATGTTGGAGAAGTAGTTAAACGCTCTGTCGCTGCACTTTTTGTTGATCCATTGCCACTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

509

Amino Acids

59.11

Weight (kDa)

5.03

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 1 - 154 7.3e-50 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 211 - 451 3e-89 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 251 - 451 7.4e-52 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 217, 871, 891
AciI CCGC 2 cut(s) 536, 1223
AclWI GGATC 1 cut(s) 1505
AcsI RAATTY 2 cut(s) 263, 1457
AfaI GTAC 6 cut(s) 30, 499, 802, 881, 959, 1169
AfeI AGCGCT 1 cut(s) 549
AfiI CCNNNNNNNGG 6 cut(s) 109, 409, 465, 727, 1015, 1051
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 1 cut(s) 500
Alw26I GTCTC 3 cut(s) 996, 1300, 1301
AlwI GGATC 1 cut(s) 1505
Aor51HI AGCGCT 1 cut(s) 549
AoxI GGCC 2 cut(s) 467, 1017
ApeKI GCWGC 2 cut(s) 440, 1496
ApoI RAATTY 2 cut(s) 263, 1457
AseI ATTAAT 1 cut(s) 83
AspA2I CCTAGG 1 cut(s) 103
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 2 cut(s) 187, 1018
AsuHPI GGTGA 2 cut(s) 191, 382
AvaII GGWCC 1 cut(s) 187
AvrII CCTAGG 1 cut(s) 103
BarI GAAGNNNNNNTAC 2 cut(s) 1171, 1203
BbvI GCAGC 2 cut(s) 427, 1483
BccI CCATC 2 cut(s) 170, 691
BciT130I CCWGG 1 cut(s) 502
BciVI GTATCC 1 cut(s) 1052
BcoDI GTCTC 3 cut(s) 996, 1300, 1301
BcuI ACTAGT 1 cut(s) 31
BfmI CTRYAG 1 cut(s) 1101
BfoI RGCGCY 1 cut(s) 551
BfuI GTATCC 1 cut(s) 1052
BisI GCNGC 2 cut(s) 441, 1497
BlnI CCTAGG 1 cut(s) 103
BlsI GCNGC 2 cut(s) 442, 1498
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 502
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 187, 1018
BmiI GGNNCC 1 cut(s) 1028
BmrFI CCNGG 1 cut(s) 502
BmsI GCATC 3 cut(s) 62, 781, 1330
BoxI GACNNNNGTC 1 cut(s) 1103
BpuEI CTTGAG 2 cut(s) 438, 800
Bsa29I ATCGAT 1 cut(s) 1314
BsaBI GATNNNNATC 2 cut(s) 180, 576
BsaI GGTCTC 1 cut(s) 1300
BsaJI CCNNGG 3 cut(s) 103, 875, 1140
BsaXI ACNNNNNCTCC 2 cut(s) 1464, 1494
Bsc4I CCNNNNNNNGG 6 cut(s) 109, 409, 465, 727, 1015, 1051
Bse3DI GCAATG 2 cut(s) 1218, 1514
Bse8I GATNNNNATC 2 cut(s) 180, 576
BseBI CCWGG 1 cut(s) 502
BseCI ATCGAT 1 cut(s) 1314
BseDI CCNNGG 3 cut(s) 103, 875, 1140
BseGI GGATG 5 cut(s) 702, 784, 1043, 1219, 1258
BseJI GATNNNNATC 2 cut(s) 180, 576
BseLI CCNNNNNNNGG 6 cut(s) 109, 409, 465, 727, 1015, 1051
BseMI GCAATG 2 cut(s) 1218, 1514
BseMII CTCAG 1 cut(s) 1311
BseRI GAGGAG 2 cut(s) 617, 1070
BseXI GCAGC 2 cut(s) 427, 1483
BsgI GTGCAG 1 cut(s) 1482
BshFI GGCC 2 cut(s) 469, 1019
BshVI ATCGAT 1 cut(s) 1314
BslI CCNNNNNNNGG 6 cut(s) 109, 409, 465, 727, 1015, 1051
BsmAI GTCTC 3 cut(s) 996, 1300, 1301
BsnI GGCC 2 cut(s) 469, 1019
Bso31I GGTCTC 1 cut(s) 1300
Bsp143I GATC 3 cut(s) 181, 1432, 1510
BspACI CCGC 2 cut(s) 536, 1223
BspANI GGCC 2 cut(s) 469, 1019
BspCNI CTCAG 1 cut(s) 1310
BspDI ATCGAT 1 cut(s) 1314
BspLI GGNNCC 1 cut(s) 1028
BspPI GGATC 1 cut(s) 1505
BspQI GCTCTTC 1 cut(s) 927
BspTNI GGTCTC 1 cut(s) 1300
BsrDI GCAATG 2 cut(s) 1218, 1514
BssECI CCNNGG 3 cut(s) 103, 875, 1140
BssMI GATC 3 cut(s) 181, 1432, 1510
BssNAI GTATAC 1 cut(s) 892
BssT1I CCWWGG 1 cut(s) 103
Bst1107I GTATAC 1 cut(s) 892
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 5 cut(s) 281, 756, 879, 1102, 1381
Bst6I CTCTTC 2 cut(s) 210, 927
BstDEI CTNAG 5 cut(s) 221, 313, 347, 608, 1297
BstDSI CCRYGG 1 cut(s) 875
BstENI CCTNNNNNAGG 1 cut(s) 1013
BstF5I GGATG 5 cut(s) 702, 784, 1043, 1219, 1258
BstH2I RGCGCY 1 cut(s) 551
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 3 cut(s) 184, 1435, 1513
BstMAI GTCTC 3 cut(s) 996, 1300, 1301
BstMBI GATC 3 cut(s) 181, 1432, 1510
BstMWI GCNNNNNNNGC 2 cut(s) 943, 980
BstNI CCWGG 1 cut(s) 502
BstNSI RCATGY 3 cut(s) 798, 886, 1070
BstPAI GACNNNNGTC 1 cut(s) 1103
BstSCI CCNGG 1 cut(s) 500
BstSFI CTRYAG 1 cut(s) 1101
BstV1I GCAGC 2 cut(s) 427, 1483
BstXI CCANNNNNNTGG 1 cut(s) 176
BstZ17I GTATAC 1 cut(s) 892
Bsu15I ATCGAT 1 cut(s) 1314
BsuI GTATCC 1 cut(s) 1052
BsuRI GGCC 2 cut(s) 469, 1019
BsuTUI ATCGAT 1 cut(s) 1314
BtgI CCRYGG 1 cut(s) 875
BtsCI GGATG 5 cut(s) 702, 784, 1043, 1219, 1258
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 2 cut(s) 187, 1018
ClaI ATCGAT 1 cut(s) 1314
Csp6I GTAC 6 cut(s) 29, 498, 801, 880, 958, 1168
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviQI GTAC 6 cut(s) 29, 498, 801, 880, 958, 1168
DdeI CTNAG 5 cut(s) 221, 313, 347, 608, 1297
DpnI GATC 3 cut(s) 183, 1434, 1512
DpnII GATC 3 cut(s) 181, 1432, 1510
DraI TTTAAA 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 210, 927
EarI CTCTTC 2 cut(s) 210, 927
Eco130I CCWWGG 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 469
Eco31I GGTCTC 1 cut(s) 1300
Eco32I GATATC 2 cut(s) 310, 784
Eco47I GGWCC 1 cut(s) 187
Eco47III AGCGCT 1 cut(s) 549
EcoNI CCTNNNNNAGG 1 cut(s) 1013
EcoRI GAATTC 1 cut(s) 1457
EcoRII CCWGG 1 cut(s) 500
EcoRV GATATC 2 cut(s) 310, 784
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 3 cut(s) 796, 1068, 1345
ErhI CCWWGG 1 cut(s) 103
FalI AAGNNNNNCTT 6 cut(s) 281, 313, 366, 398, 591, 623
FauI CCCGC 1 cut(s) 1216
FauNDI CATATG 2 cut(s) 58, 1465
FblI GTMKAC 3 cut(s) 217, 871, 891
Fnu4HI GCNGC 2 cut(s) 441, 1497
FokI GGATG 5 cut(s) 709, 791, 1050, 1226, 1265
Fsp4HI GCNGC 2 cut(s) 441, 1497
GlaI GCGC 1 cut(s) 549
GluI GCNGC 2 cut(s) 441, 1497
HaeII RGCGCY 1 cut(s) 551
HaeIII GGCC 2 cut(s) 469, 1019
HhaI GCGC 1 cut(s) 550
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 2 cut(s) 838, 853
HindII GTYRAC 2 cut(s) 838, 853
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 4 cut(s) 532, 652, 963, 991
HphI GGTGA 2 cut(s) 191, 382
Hpy166II GTNNAC 9 cut(s) 218, 277, 803, 838, 853, 872, 892, 967, 1441
Hpy188I TCNGA 4 cut(s) 250, 746, 1073, 1300
Hpy188III TCNNGA 4 cut(s) 185, 485, 817, 827
Hpy8I GTNNAC 9 cut(s) 218, 277, 803, 838, 853, 872, 892, 967, 1441
HpyAV CCTTC 3 cut(s) 31, 1028, 1342
HpyCH4III ACNGT 5 cut(s) 281, 756, 879, 1102, 1381
HpyF10VI GCNNNNNNNGC 2 cut(s) 943, 980
HpyF3I CTNAG 5 cut(s) 221, 313, 347, 608, 1297
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 3 cut(s) 181, 1432, 1510
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 2 cut(s) 604, 1032
LpnPI CCDG 6 cut(s) 170, 487, 514, 566, 826, 1421
Lsp1109I GCAGC 2 cut(s) 427, 1483
LweI GCATC 3 cut(s) 62, 781, 1330
MaeIII GTNAC 2 cut(s) 281, 1274
MalI GATC 3 cut(s) 183, 1434, 1512
MboI GATC 3 cut(s) 181, 1432, 1510
MlyI GAGTC 2 cut(s) 526, 972
MmeI TCCRAC 3 cut(s) 121, 712, 1450
Mph1103I ATGCAT 3 cut(s) 796, 1068, 1345
MseI TTAA 9 cut(s) 83, 162, 267, 299, 1115, 1323, 1401, 1413, 1482
MslI CAYNNNNRTG 3 cut(s) 174, 1153, 1525
MspR9I CCNGG 1 cut(s) 502
MvaI CCWGG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 943, 980
NdeI CATATG 2 cut(s) 58, 1465
NdeII GATC 3 cut(s) 181, 1432, 1510
NlaIV GGNNCC 1 cut(s) 1028
NsiI ATGCAT 3 cut(s) 796, 1068, 1345
NspI RCATGY 3 cut(s) 798, 886, 1070
PceI AGGCCT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 927
PfeI GAWTC 2 cut(s) 652, 991
PkrI GCNGC 2 cut(s) 442, 1498
PleI GAGTC 2 cut(s) 526, 971
PpsI GAGTC 2 cut(s) 526, 971
PshAI GACNNNNGTC 1 cut(s) 1103
PshBI ATTAAT 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 1 cut(s) 1028
PspPI GGNCC 2 cut(s) 187, 1018
PsrI GAACNNNNNNTAC 2 cut(s) 12, 44
RsaI GTAC 6 cut(s) 30, 499, 802, 881, 959, 1169
RsaNI GTAC 6 cut(s) 29, 498, 801, 880, 958, 1168
RseI CAYNNNNRTG 3 cut(s) 174, 1153, 1525
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 9 cut(s) 83, 162, 267, 299, 1115, 1323, 1401, 1413, 1482
SatI GCNGC 2 cut(s) 441, 1497
Sau3AI GATC 3 cut(s) 181, 1432, 1510
Sau96I GGNCC 2 cut(s) 187, 1018
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 2 cut(s) 526, 972
ScrFI CCNGG 1 cut(s) 502
SfaNI GCATC 3 cut(s) 62, 781, 1330
SfcI CTRYAG 1 cut(s) 1101
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 3 cut(s) 174, 1153, 1525
SmlI CTYRAG 2 cut(s) 453, 815
SmoI CTYRAG 2 cut(s) 453, 815
SpeI ACTAGT 1 cut(s) 31
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 2 cut(s) 536, 1223
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 1 cut(s) 500
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 5 cut(s) 281, 756, 879, 1102, 1381
TaqI TCGA 4 cut(s) 1190, 1314, 1372, 1524
TaqII GACCGA 1 cut(s) 1397
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 2 cut(s) 652, 991
Tru1I TTAA 9 cut(s) 83, 162, 267, 299, 1115, 1323, 1401, 1413, 1482
Tru9I TTAA 9 cut(s) 83, 162, 267, 299, 1115, 1323, 1401, 1413, 1482
TseI GCWGC 2 cut(s) 440, 1496
VpaK11BI GGWCC 1 cut(s) 187
VspI ATTAAT 1 cut(s) 83
XagI CCTNNNNNAGG 1 cut(s) 1013
XapI RAATTY 2 cut(s) 263, 1457
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 3 cut(s) 798, 886, 1070
XmaJI CCTAGG 1 cut(s) 103
XmiI GTMKAC 3 cut(s) 217, 871, 891
ZrmI AGTACT 1 cut(s) 30
Zsp2I ATGCAT 3 cut(s) 796, 1068, 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.