Rorug04G0000300

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000529
Physical Location & Seq
Reverse (-)
83147 .. 87139
3993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0000300.1

Sequence Viewer

Length: 1197 bp
ATGATGCAGTTTTCAGTTTTGGAAAGTTTATTGTTGCTGTGGTTATTGTGTAATTTTTTCTCAGGTGATGAAATGACGAGGATCATATGGACAGCAATCAAAGAAAAACTTATTTTCCCCGTGGATTTGGACATGAAGTATTATGATTTAGGGATTGTTAACCGTGATGCTACAGATGACAAATTTACGGTAGAGAGTGCCAAAGCCACTCTTAAGTTCAATGTTGCTGTGAAATGTGCTACAATAACTCCTGATGAGACCAGAATGAAGGAGTTTGGGCTAAAATCAACGTGGAGGAGTCCCAATGGCACAATTAAAAACATTCTAAATGGTACTGTCTTTCGTGAACCTATCCTATGCCGAAACATTCCTAGAATAGTTCCTGGTAAGGCTGGAAGAAACCCATATGTATTGGTAGGCATGCTTTCGGCGATCAGTATCGACTATCGAGCTACTGATACAGTTATCACGGGACCAGGAAAGCTGAAAATGGTATTTGTCTCAGAAGGAGGAAACACCCCTACGGAGCTTAATGTCTATCATTTCAAAGGTCCTGGTGTGGCCCTCGCTATGTATAATGTTGATGAGTGGCCACTATACTTGAGCACCAAAAACACAATTCTTAAGAAATATGACGGCAGGTTTAAGGACATATTGGAGGAGGTTTATGTGGAAAAGTGGAAGCAGAAGTTTGAAGAAAACTCTATATGGTATGAGCATCGGCTAATAGATGACATGGTGGCTTATGCACTCAAAAGTGAGGGTGGATATGTTTGGGCTTGCAAAAACTACGATGGAGATGTCCAGAGTGATTTGCTTGCTCAAGGATTTGGCTCTTTGGGCCTCATGACTTCTGTGGTGCTATCCTCAGATGGGAAAACAATAGAAGCTGAAGCAGCTCATGGGACAGTAACTTGTCATTTTCGGTTACATCAGAAGGGACAAGAAACCAGTACAAACAGTATTGCTTCAATATTTGCATGGACAAGAGGCCTAGAGCATAGGTTGCTAGATTTTGTTCAAAAGTTGGAGGCTGCGTGCATTGAGACATTGGAGGCAGGAAACATGACTAAGGATCTCGCCTTATTGATCCATGGCCCCAAGGTATCAAGGGAGTTCTATTTGAACACAGAAGAGTTCATTGACACAGTCGCAAAGAATGTTGAGCAAAAGCTTCGGGAACCTGCTCTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

398

Amino Acids

44.92

Weight (kDa)

5.91

Isoelectric Point (pI)

24.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 21 - 384 8.8e-54 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 630, 1192
AclWI GGATC 3 cut(s) 89, 1083, 1084
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 912
AfaI GTAC 2 cut(s) 334, 955
AfiI CCNNNNNNNGG 1 cut(s) 873
AflII CTTAAG 2 cut(s) 212, 623
AgsI TTSAA 6 cut(s) 220, 547, 695, 972, 1022, 1126
AjnI CCWGG 3 cut(s) 382, 475, 553
AluBI AGCT 6 cut(s) 452, 484, 529, 890, 899, 1174
AluI AGCT 6 cut(s) 452, 484, 529, 890, 899, 1174
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 3 cut(s) 251, 505, 1040
AlwI GGATC 3 cut(s) 89, 1083, 1084
AoxI GGCC 5 cut(s) 561, 590, 841, 991, 1096
ApeKI GCWGC 2 cut(s) 896, 1034
ApoI RAATTY 1 cut(s) 182
AspS9I GGNCC 5 cut(s) 473, 551, 562, 841, 1097
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 2 cut(s) 473, 551
BalI TGGCCA 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 2 cut(s) 908, 1021
BccI CCATC 2 cut(s) 788, 866
BceAI ACGGC 1 cut(s) 652
BcgI CGANNNNNNTGC 2 cut(s) 1157, 1191
BciT130I CCWGG 3 cut(s) 384, 477, 555
BcoDI GTCTC 3 cut(s) 251, 505, 1040
BfaI CTAG 3 cut(s) 372, 995, 1010
BfmI CTRYAG 1 cut(s) 171
BfrI CTTAAG 2 cut(s) 212, 623
BfuAI ACCTGC 2 cut(s) 630, 1192
BisI GCNGC 2 cut(s) 897, 1035
BlsI GCNGC 2 cut(s) 898, 1036
Bme1390I CCNGG 3 cut(s) 384, 477, 555
Bme18I GGWCC 2 cut(s) 473, 551
BmgT120I GGNCC 5 cut(s) 473, 551, 562, 841, 1097
BmiI GGNNCC 3 cut(s) 474, 1099, 1182
BmrFI CCNGG 3 cut(s) 384, 477, 555
BmsI GCATC 2 cut(s) 157, 727
BpuEI CTTGAG 2 cut(s) 622, 807
BsaBI GATNNNNATC 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 120, 1093, 1101
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 1022, 1052
Bsc4I CCNNNNNNNGG 1 cut(s) 873
Bse1I ACTGG 1 cut(s) 951
Bse8I GATNNNNATC 1 cut(s) 437
BseBI CCWGG 3 cut(s) 384, 477, 555
BseDI CCNNGG 3 cut(s) 120, 1093, 1101
BseJI GATNNNNATC 1 cut(s) 437
BseLI CCNNNNNNNGG 1 cut(s) 873
BseMII CTCAG 3 cut(s) 75, 516, 882
BseNI ACTGG 1 cut(s) 951
BseRI GAGGAG 2 cut(s) 310, 674
BseXI GCAGC 2 cut(s) 908, 1021
BshFI GGCC 5 cut(s) 563, 592, 843, 993, 1098
BsiHKAI GWGCWC 1 cut(s) 608
BslFI GGGAC 4 cut(s) 285, 486, 919, 954
BslI CCNNNNNNNGG 1 cut(s) 873
BsmAI GTCTC 3 cut(s) 251, 505, 1040
BsmFI GGGAC 4 cut(s) 285, 486, 919, 954
BsnI GGCC 5 cut(s) 563, 592, 843, 993, 1098
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 608
Bsp143I GATC 4 cut(s) 81, 432, 1075, 1089
Bsp19I CCATGG 1 cut(s) 1093
BspANI GGCC 5 cut(s) 563, 592, 843, 993, 1098
BspCNI CTCAG 3 cut(s) 74, 515, 881
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 3 cut(s) 474, 1099, 1182
BspMI ACCTGC 2 cut(s) 630, 1192
BspPI GGATC 3 cut(s) 89, 1083, 1084
BspTI CTTAAG 2 cut(s) 212, 623
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 951
BssECI CCNNGG 3 cut(s) 120, 1093, 1101
BssMI GATC 4 cut(s) 81, 432, 1075, 1089
BssT1I CCWWGG 2 cut(s) 1093, 1101
Bst2UI CCWGG 3 cut(s) 384, 477, 555
Bst4CI ACNGT 7 cut(s) 164, 190, 337, 463, 910, 962, 1150
Bst6I CTCTTC 1 cut(s) 1128
BstAFI CTTAAG 2 cut(s) 212, 623
BstAPI GCANNNNNTGC 1 cut(s) 1006
BstC8I GCNNGC 4 cut(s) 422, 781, 819, 1039
BstDEI CTNAG 4 cut(s) 61, 502, 868, 1071
BstDSI CCRYGG 2 cut(s) 120, 1093
BstKTI GATC 4 cut(s) 84, 435, 1078, 1092
BstMAI GTCTC 3 cut(s) 251, 505, 1040
BstMBI GATC 4 cut(s) 81, 432, 1075, 1089
BstMWI GCNNNNNNNGC 3 cut(s) 840, 896, 1006
BstNI CCWGG 3 cut(s) 384, 477, 555
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 382, 475, 553
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 908, 1021
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BsuRI GGCC 5 cut(s) 563, 592, 843, 993, 1098
BtgI CCRYGG 2 cut(s) 120, 1093
BveI ACCTGC 2 cut(s) 630, 1192
Cac8I GCNNGC 4 cut(s) 422, 781, 819, 1039
CciI TCATGA 1 cut(s) 846
Cfr13I GGNCC 5 cut(s) 473, 551, 562, 841, 1097
Csp6I GTAC 2 cut(s) 333, 954
CspCI CAANNNNNGTGG 2 cut(s) 582, 617
CviAII CATG 8 cut(s) 133, 421, 736, 847, 902, 981, 1066, 1094
CviQI GTAC 2 cut(s) 333, 954
DdeI CTNAG 4 cut(s) 61, 502, 868, 1071
DpnI GATC 4 cut(s) 83, 434, 1077, 1091
DpnII GATC 4 cut(s) 81, 432, 1075, 1089
EaeI YGGCCR 1 cut(s) 590
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
Eco130I CCWWGG 2 cut(s) 1093, 1101
Eco147I AGGCCT 1 cut(s) 993
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 473, 551
Eco57I CTGAAG 1 cut(s) 912
EcoO109I RGGNCCY 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 382, 475, 553
EcoT14I CCWWGG 2 cut(s) 1093, 1101
ErhI CCWWGG 2 cut(s) 1093, 1101
FaeI CATG 8 cut(s) 136, 424, 739, 850, 905, 984, 1069, 1097
FalI AAGNNNNNCTT 4 cut(s) 93, 125, 195, 227
FaqI GGGAC 4 cut(s) 285, 486, 919, 954
FatI CATG 8 cut(s) 132, 420, 735, 846, 901, 980, 1065, 1093
FauNDI CATATG 2 cut(s) 86, 406
Fnu4HI GCNGC 2 cut(s) 897, 1035
Fsp4HI GCNGC 2 cut(s) 897, 1035
FspBI CTAG 3 cut(s) 372, 995, 1010
GluI GCNGC 2 cut(s) 897, 1035
HaeIII GGCC 5 cut(s) 563, 592, 843, 993, 1098
Hin1II CATG 8 cut(s) 136, 424, 739, 850, 905, 984, 1069, 1097
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 1172
HinfI GANTC 1 cut(s) 298
HpaI GTTAAC 1 cut(s) 160
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 2 cut(s) 160, 347
Hpy188I TCNGA 3 cut(s) 505, 871, 936
Hpy188III TCNNGA 5 cut(s) 251, 344, 805, 847, 1178
Hpy8I GTNNAC 2 cut(s) 160, 347
HpyAV CCTTC 3 cut(s) 262, 500, 931
HpyCH4III ACNGT 7 cut(s) 164, 190, 337, 463, 910, 962, 1150
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 5 cut(s) 7, 749, 783, 980, 1041
HpyF10VI GCNNNNNNNGC 3 cut(s) 840, 896, 1006
HpyF3I CTNAG 4 cut(s) 61, 502, 868, 1071
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 8 cut(s) 136, 424, 739, 850, 905, 984, 1069, 1097
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 4 cut(s) 81, 432, 1075, 1089
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 2 cut(s) 908, 1021
LweI GCATC 2 cut(s) 157, 727
MaeI CTAG 3 cut(s) 372, 995, 1010
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 910, 927
MalI GATC 4 cut(s) 83, 434, 1077, 1091
MboI GATC 4 cut(s) 81, 432, 1075, 1089
MboII GAAGA 3 cut(s) 408, 707, 1145
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 1 cut(s) 608
MlsI TGGCCA 1 cut(s) 592
MluCI AATT 4 cut(s) 52, 182, 312, 618
MluNI TGGCCA 1 cut(s) 592
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 1 cut(s) 1008
Mox20I TGGCCA 1 cut(s) 592
MscI TGGCCA 1 cut(s) 592
MseI TTAA 6 cut(s) 159, 213, 315, 531, 624, 645
Msp20I TGGCCA 1 cut(s) 592
MspCI CTTAAG 2 cut(s) 212, 623
MspR9I CCNGG 3 cut(s) 384, 477, 555
MvaI CCWGG 3 cut(s) 384, 477, 555
MwoI GCNNNNNNNGC 3 cut(s) 840, 896, 1006
NcoI CCATGG 1 cut(s) 1093
NdeI CATATG 2 cut(s) 86, 406
NdeII GATC 4 cut(s) 81, 432, 1075, 1089
NlaIII CATG 8 cut(s) 136, 424, 739, 850, 905, 984, 1069, 1097
NlaIV GGNNCC 3 cut(s) 474, 1099, 1182
NspI RCATGY 1 cut(s) 424
PaeI GCATGC 1 cut(s) 424
PagI TCATGA 1 cut(s) 846
PceI AGGCCT 1 cut(s) 993
PflFI GACNNNGTC 1 cut(s) 1148
PkrI GCNGC 2 cut(s) 898, 1036
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
PpuMI RGGWCCY 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 3 cut(s) 382, 475, 553
PspGI CCWGG 3 cut(s) 382, 475, 553
PspN4I GGNNCC 3 cut(s) 474, 1099, 1182
PspPI GGNCC 5 cut(s) 473, 551, 562, 841, 1097
PspPPI RGGWCCY 1 cut(s) 551
PsuI RGATCY 1 cut(s) 1075
PsyI GACNNNGTC 1 cut(s) 1148
RsaI GTAC 2 cut(s) 334, 955
RsaNI GTAC 2 cut(s) 333, 954
SaqAI TTAA 6 cut(s) 159, 213, 315, 531, 624, 645
SatI GCNGC 2 cut(s) 897, 1035
Sau3AI GATC 4 cut(s) 81, 432, 1075, 1089
Sau96I GGNCC 5 cut(s) 473, 551, 562, 841, 1097
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 3 cut(s) 384, 477, 555
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 2 cut(s) 157, 727
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 2 cut(s) 473, 551
SmlI CTYRAG 4 cut(s) 212, 601, 623, 822
SmoI CTYRAG 4 cut(s) 212, 601, 623, 822
SphI GCATGC 1 cut(s) 424
Sse9I AATT 4 cut(s) 52, 182, 312, 618
SseBI AGGCCT 1 cut(s) 993
SspI AATATT 1 cut(s) 975
SspMI CTAG 3 cut(s) 372, 995, 1010
StuI AGGCCT 1 cut(s) 993
StyD4I CCNGG 3 cut(s) 382, 475, 553
StyI CCWWGG 2 cut(s) 1093, 1101
TaaI ACNGT 7 cut(s) 164, 190, 337, 463, 910, 962, 1150
TaiI ACGT 1 cut(s) 293
TaqI TCGA 2 cut(s) 441, 448
TasI AATT 4 cut(s) 52, 182, 312, 618
TatI WGTACW 1 cut(s) 953
Tru1I TTAA 6 cut(s) 159, 213, 315, 531, 624, 645
Tru9I TTAA 6 cut(s) 159, 213, 315, 531, 624, 645
TseI GCWGC 2 cut(s) 896, 1034
TspDTI ATGAA 4 cut(s) 84, 149, 281, 1129
TspGWI ACGGA 1 cut(s) 539
Tth111I GACNNNGTC 1 cut(s) 1148
Vha464I CTTAAG 2 cut(s) 212, 623
VpaK11BI GGWCC 2 cut(s) 473, 551
XapI RAATTY 1 cut(s) 182
XceI RCATGY 1 cut(s) 424
XspI CTAG 3 cut(s) 372, 995, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.