Rroxscaffold_5G00338730

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000529
Physical Location & Seq
Forward (+)
6982537 .. 6985021
2485 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00338730.1

Sequence Viewer

Length: 606 bp
ATGGGTATTATGGTTCCCTCTTTGGCTGGTGCATCAGCTTTAGAATATGGCAGTAGATCTTTGAGAAAGCTTGGTTCATCTTATTTGGGTGGGGATGATGATACCGATGGCAAGCTGGATGAATCTTCAACAAAGTATGGGCAGGAAGATGGAGATGATGATGTTACAGTGAAGAGCTTCCCGGTTTGTGATGATCGCCATTCGGAACTTATCCCTTGCTTGGACAGAAATCTCATATATCAAATAAGACTGAAGTTGGACCTGTCTTTGATGGAGCACTATGAGAGGCATTGTCCTTCTCCAGAAAGGCGATACAATTGCTTGATTCCTCCTCCAATAGGGTACAAGGTCCCAATCAAATGGCCCCAGAGCAGAGATGAAGTATGGAAAGCAAATATACCTCATACTCACCTTGCACATGAGAAATCCGACCAGAACTGGATGGTTGAAAAAGGTGACAAGATTAGTTTTCCTGGGGGAGGCACACATTTTCACTATGGAGCTGATAAGTATATTGCTTCAATTGCAAATGTAAGATTGCTTCCTCGAAACTGTCTTTGCCAAAAAGGATGGTGTAGGCGTGTAGCACTAATTGACAGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

201

Amino Acids

22.78

Weight (kDa)

6.7

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 69 - 178 4.5e-45 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 272
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 220, 338, 479
AgsI TTSAA 3 cut(s) 129, 449, 522
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 5 cut(s) 38, 70, 115, 177, 503
AluI AGCT 5 cut(s) 38, 70, 115, 177, 503
Alw21I GWGCWC 1 cut(s) 279
AoxI GGCC 1 cut(s) 362
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 259, 349, 363
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 2 cut(s) 401, 467
AvaII GGWCC 2 cut(s) 259, 349
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
Bbv12I GWGCWC 1 cut(s) 279
BccI CCATC 5 cut(s) 101, 143, 265, 436, 564
BcgI CGANNNNNNTGC 2 cut(s) 300, 334
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 182
BglII AGATCT 1 cut(s) 56
Bme1390I CCNGG 2 cut(s) 182, 474
Bme18I GGWCC 2 cut(s) 259, 349
BmgT120I GGNCC 3 cut(s) 259, 349, 363
BmiI GGNNCC 3 cut(s) 15, 351, 365
BmrFI CCNGG 2 cut(s) 182, 474
BmsI GCATC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 473
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 338, 479
Bse1I ACTGG 1 cut(s) 443
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 473
BseGI GGATG 4 cut(s) 100, 124, 447, 575
BseLI CCNNNNNNNGG 3 cut(s) 220, 338, 479
BseNI ACTGG 1 cut(s) 443
BseRI GAGGAG 1 cut(s) 321
BshFI GGCC 1 cut(s) 364
BsiHKAI GWGCWC 1 cut(s) 279
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 3 cut(s) 220, 338, 479
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 1 cut(s) 364
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 2 cut(s) 56, 193
BspANI GGCC 1 cut(s) 364
BspLI GGNNCC 3 cut(s) 15, 351, 365
BspQI GCTCTTC 1 cut(s) 167
BsrI ACTGG 1 cut(s) 443
BssECI CCNNGG 1 cut(s) 473
BssMI GATC 2 cut(s) 56, 193
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 169, 554
Bst6I CTCTTC 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 113
BstENI CCTNNNNNAGG 2 cut(s) 336, 477
BstF5I GGATG 4 cut(s) 100, 124, 447, 575
BstKTI GATC 2 cut(s) 59, 196
BstMBI GATC 2 cut(s) 56, 193
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 180, 472
BstX2I RGATCY 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 360
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 1 cut(s) 364
BtsCI GGATG 4 cut(s) 100, 124, 447, 575
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 113
Cfr13I GGNCC 3 cut(s) 259, 349, 363
Csp6I GTAC 1 cut(s) 343
CviAII CATG 1 cut(s) 419
CviJI RGCY 7 cut(s) 26, 38, 70, 115, 177, 364, 503
CviKI_1 RGCY 7 cut(s) 26, 38, 70, 115, 177, 364, 503
CviQI GTAC 1 cut(s) 343
DpnI GATC 2 cut(s) 58, 195
DpnII GATC 2 cut(s) 56, 193
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Eco47I GGWCC 2 cut(s) 259, 349
Eco57I CTGAAG 1 cut(s) 272
EcoNI CCTNNNNNAGG 2 cut(s) 336, 477
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 472
FaeI CATG 1 cut(s) 422
FaqI GGGAC 1 cut(s) 335
FatI CATG 1 cut(s) 418
FokI GGATG 4 cut(s) 107, 131, 454, 582
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 1 cut(s) 364
HapII CCGG 1 cut(s) 182
Hin1II CATG 1 cut(s) 422
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 2 cut(s) 122, 325
HpaII CCGG 1 cut(s) 182
HphI GGTGA 2 cut(s) 401, 467
Hpy188I TCNGA 2 cut(s) 205, 430
Hpy188III TCNNGA 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 2 cut(s) 169, 554
HpyCH4V TGCA 3 cut(s) 32, 416, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
Hsp92II CATG 1 cut(s) 422
Kzo9I GATC 2 cut(s) 56, 193
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 2 cut(s) 274, 500
LweI GCATC 1 cut(s) 41
MaeIII GTNAC 2 cut(s) 163, 455
MalI GATC 2 cut(s) 58, 195
MboI GATC 2 cut(s) 56, 193
MboII GAAGA 3 cut(s) 117, 158, 184
MfeI CAATTG 2 cut(s) 316, 522
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 3 cut(s) 316, 522, 591
MmeI TCCRAC 2 cut(s) 237, 453
MnlI CCTC 7 cut(s) 28, 279, 339, 342, 411, 473, 555
MroXI GAANNNNTTC 1 cut(s) 176
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 182, 474
MunI CAATTG 2 cut(s) 316, 522
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 524
NciI CCSGG 1 cut(s) 182
NdeII GATC 2 cut(s) 56, 193
NlaIII CATG 1 cut(s) 422
NlaIV GGNNCC 3 cut(s) 15, 351, 365
NmuCI GTSAC 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 122, 325
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 15, 351, 365
PspPI GGNCC 3 cut(s) 259, 349, 363
PspPPI RGGWCCY 1 cut(s) 349
PsuI RGATCY 1 cut(s) 56
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SapI GCTCTTC 1 cut(s) 167
Sau3AI GATC 2 cut(s) 56, 193
Sau96I GGNCC 3 cut(s) 259, 349, 363
ScrFI CCNGG 2 cut(s) 182, 474
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 1 cut(s) 41
SinI GGWCC 2 cut(s) 259, 349
Sse9I AATT 3 cut(s) 316, 522, 591
StyD4I CCNGG 2 cut(s) 180, 472
TaaI ACNGT 2 cut(s) 169, 554
TaqI TCGA 1 cut(s) 547
TasI AATT 3 cut(s) 316, 522, 591
TfiI GAWTC 2 cut(s) 122, 325
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 3 cut(s) 66, 135, 393
TspRI CASTG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 259, 349
XagI CCTNNNNNAGG 2 cut(s) 336, 477
XmnI GAANNNNTTC 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.