Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000529

Physical Location & Seq

Genomic Coordinates Forward (+)

20398891 .. 20400371

Gene Structure 1481 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1215000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 813 bp

ATGATGAGAGAGAGATCTGATCCGGCTCAAAAGAAGTGTATAATCACATCTCTGTGTATAGTGGCAATCCTTCTTGGTTTCCTATATGTGTATTATGGATCCCTTTTTAGCTCTCAAAGTCACGGTGCATCAGCACTAGAATATGGTAGCAGATCTTTGAGAAAGCTTGGTTCATCTTGTTTGGGTGAGGATGAAGATAACAATAAGAAGCAAGATGAATCTTCAACAAAATATGGGCAGGAAAATGGAGAGGACGATGTCACATTGAAGAACCTCCTTATGGAGCACTATGAAAGACATTGCCCTACCCTAGAAAGGCGTTTCAATTGCTTGATTCCTCCTCCACCAAGGTCCCAATCAAATGGCCCCAAAGCAGAGATGAAGTTTCAAAAGCAAATATACCTCATACTCACCTTGCACATGAGAAATCCAATCAAAACTAGATGGTTAAAAAAGGTGAAAAGATATAGTTTCCTAGGGGAGGCACACATTTCCACTGTGGAGCTGATAGTTTTTTATGTTGGCTATGAAGTTGCAAGTTTTGGAGCATATTTACTTTCATTTGATATTATAGTAATTTCATTAGCACCCAATGATGGGCATCAAAACCTGATCCAATTTGCTTTAGAAATAGAAATTCCAGCCTTTTTTGGTGTTCTAGGAATCAAAAGGCTTCCTTACCCTAGCAAATCCTTTGAACTTGCTCATTGTTCCCGCTGTAGAATTGATTGGCTTCAACAAGATGAAATCCTCCTTCTTAAGCTGGATAGGTTGATCAGACCAGGAGGCCACTTTGCGTACTCATCTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005911 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009506 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0030054 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

271

Amino Acids

30.86

Weight (kDa)

8.58

Isoelectric Point (pI)

51.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	170 - 269	3.9e-42	Putative S-adenosyl-L-methionine-dependent methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000529)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca	FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica	MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica	Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis	pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis	RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata	RLG00000009742 RLG00000012463
rosa_multiflora	Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii	Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa	Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha	Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana	Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	715
AclWI	GGATC	4 cut(s)	14, 93, 106, 607
AcsI	RAATTY	1 cut(s)	636
AfaI	GTAC	1 cut(s)	800
AfiI	CCNNNNNNNGG	5 cut(s)	280, 315, 481, 596, 597
AflII	CTTAAG	1 cut(s)	758
AgsI	TTSAA	6 cut(s)	225, 268, 325, 389, 698, 737
AjnI	CCWGG	1 cut(s)	781
AluBI	AGCT	4 cut(s)	111, 166, 505, 763
AluI	AGCT	4 cut(s)	111, 166, 505, 763
Alw21I	GWGCWC	1 cut(s)	288
AlwI	GGATC	4 cut(s)	14, 93, 106, 607
AoxI	GGCC	2 cut(s)	364, 787
ApoI	RAATTY	1 cut(s)	636
AspA2I	CCTAGG	1 cut(s)	475
AspS9I	GGNCC	2 cut(s)	351, 365
AsuHPI	GGTGA	3 cut(s)	197, 403, 469
AvaII	GGWCC	1 cut(s)	351
AvrII	CCTAGG	1 cut(s)	475
BamHI	GGATCC	1 cut(s)	98
Bbv12I	GWGCWC	1 cut(s)	288
BccI	CCATC	2 cut(s)	438, 590
BciT130I	CCWGG	1 cut(s)	783
BclI	TGATCA	1 cut(s)	774
BfaI	CTAG	6 cut(s)	137, 311, 441, 476, 659, 684
BfmI	CTRYAG	1 cut(s)	718
BfrI	CTTAAG	1 cut(s)	758
BglII	AGATCT	2 cut(s)	14, 152
BlnI	CCTAGG	1 cut(s)	475
Bme1390I	CCNGG	1 cut(s)	783
Bme18I	GGWCC	1 cut(s)	351
BmgT120I	GGNCC	2 cut(s)	351, 365
BmiI	GGNNCC	3 cut(s)	100, 353, 367
BmrFI	CCNGG	1 cut(s)	783
BmsI	GCATC	2 cut(s)	137, 610
BsaBI	GATNNNNATC	1 cut(s)	600
BsaJI	CCNNGG	2 cut(s)	347, 475
Bsc4I	CCNNNNNNNGG	5 cut(s)	280, 315, 481, 596, 597
Bse3DI	GCAATG	1 cut(s)	298
Bse8I	GATNNNNATC	1 cut(s)	600
BseBI	CCWGG	1 cut(s)	783
BseDI	CCNNGG	2 cut(s)	347, 475
BseGI	GGATG	1 cut(s)	196
BseJI	GATNNNNATC	1 cut(s)	600
BseLI	CCNNNNNNNGG	5 cut(s)	280, 315, 481, 596, 597
BseMI	GCAATG	1 cut(s)	298
BseRI	GAGGAG	1 cut(s)	330
BshFI	GGCC	2 cut(s)	366, 789
BsiHKAI	GWGCWC	1 cut(s)	288
BsiSI	CCGG	1 cut(s)	23
BslFI	GGGAC	1 cut(s)	337
BslI	CCNNNNNNNGG	5 cut(s)	280, 315, 481, 596, 597
BsmFI	GGGAC	1 cut(s)	337
BsnI	GGCC	2 cut(s)	366, 789
Bsp1286I	GDGCHC	1 cut(s)	288
Bsp143I	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
BspACI	CCGC	1 cut(s)	715
BspANI	GGCC	2 cut(s)	366, 789
BspLI	GGNNCC	3 cut(s)	100, 353, 367
BspPI	GGATC	4 cut(s)	14, 93, 106, 607
BspTI	CTTAAG	1 cut(s)	758
BsrDI	GCAATG	1 cut(s)	298
BssECI	CCNNGG	2 cut(s)	347, 475
BssMI	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
BssT1I	CCWWGG	2 cut(s)	347, 475
Bst2UI	CCWGG	1 cut(s)	783
Bst4CI	ACNGT	2 cut(s)	125, 499
BstAFI	CTTAAG	1 cut(s)	758
BstENI	CCTNNNNNAGG	1 cut(s)	479
BstF5I	GGATG	1 cut(s)	196
BstKTI	GATC	6 cut(s)	17, 22, 101, 155, 615, 777
BstMBI	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
BstNI	CCWGG	1 cut(s)	783
BstSCI	CCNGG	1 cut(s)	781
BstSFI	CTRYAG	1 cut(s)	718
BstX2I	RGATCY	3 cut(s)	14, 98, 152
BstXI	CCANNNNNNTGG	1 cut(s)	362
BstYI	RGATCY	3 cut(s)	14, 98, 152
BsuRI	GGCC	2 cut(s)	366, 789
BtsCI	GGATG	1 cut(s)	196
BtsIMutI	CAGTG	1 cut(s)	495
Cfr13I	GGNCC	2 cut(s)	351, 365
Csp6I	GTAC	1 cut(s)	799
CviAII	CATG	1 cut(s)	421
CviQI	GTAC	1 cut(s)	799
DpnI	GATC	6 cut(s)	16, 21, 100, 154, 614, 776
DpnII	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
Eco130I	CCWWGG	2 cut(s)	347, 475
Eco47I	GGWCC	1 cut(s)	351
EcoNI	CCTNNNNNAGG	1 cut(s)	479
EcoO109I	RGGNCCY	1 cut(s)	351
EcoRII	CCWGG	1 cut(s)	781
EcoT14I	CCWWGG	2 cut(s)	347, 475
ErhI	CCWWGG	2 cut(s)	347, 475
FaeI	CATG	1 cut(s)	424
FalI	AAGNNNNNCTT	2 cut(s)	661, 693
FaqI	GGGAC	1 cut(s)	337
FatI	CATG	1 cut(s)	420
FauI	CCCGC	1 cut(s)	722
FbaI	TGATCA	1 cut(s)	774
FokI	GGATG	1 cut(s)	203
FspBI	CTAG	6 cut(s)	137, 311, 441, 476, 659, 684
HaeIII	GGCC	2 cut(s)	366, 789
HapII	CCGG	1 cut(s)	23
Hin1II	CATG	1 cut(s)	424
HindIII	AAGCTT	1 cut(s)	164
HinfI	GANTC	3 cut(s)	218, 334, 663
HpaII	CCGG	1 cut(s)	23
HphI	GGTGA	3 cut(s)	197, 403, 469
Hpy188I	TCNGA	2 cut(s)	19, 779
HpyAV	CCTTC	2 cut(s)	80, 764
HpyCH4III	ACNGT	2 cut(s)	125, 499
HpyCH4V	TGCA	3 cut(s)	128, 418, 536
Hsp92II	CATG	1 cut(s)	424
Ksp22I	TGATCA	1 cut(s)	774
Kzo9I	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
LmnI	GCTCC	3 cut(s)	283, 502, 545
LpnPI	CCDG	7 cut(s)	36, 224, 623, 654, 749, 768, 795
LweI	GCATC	2 cut(s)	137, 610
MaeI	CTAG	6 cut(s)	137, 311, 441, 476, 659, 684
MaeIII	GTNAC	2 cut(s)	119, 259
MalI	GATC	6 cut(s)	16, 21, 100, 154, 614, 776
MboI	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
MboII	GAAGA	3 cut(s)	206, 213, 280
MfeI	CAATTG	1 cut(s)	325
MflI	RGATCY	3 cut(s)	14, 98, 152
MhlI	GDGCHC	1 cut(s)	288
MluCI	AATT	5 cut(s)	325, 576, 617, 636, 723
MnlI	CCTC	9 cut(s)	181, 244, 284, 348, 351, 413, 475, 761, 779
MseI	TTAA	3 cut(s)	449, 759, 811
MslI	CAYNNNNRTG	1 cut(s)	52
MspA1I	CMGCKG	1 cut(s)	717
MspCI	CTTAAG	1 cut(s)	758
MspI	CCGG	1 cut(s)	23
MspR9I	CCNGG	1 cut(s)	783
MunI	CAATTG	1 cut(s)	325
MvaI	CCWGG	1 cut(s)	783
NdeII	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
NlaIII	CATG	1 cut(s)	424
NlaIV	GGNNCC	3 cut(s)	100, 353, 367
NmuCI	GTSAC	2 cut(s)	119, 259
PfeI	GAWTC	3 cut(s)	218, 334, 663
PflFI	GACNNNGTC	1 cut(s)	257
PpuMI	RGGWCCY	1 cut(s)	351
Psp5II	RGGWCCY	1 cut(s)	351
Psp6I	CCWGG	1 cut(s)	781
PspGI	CCWGG	1 cut(s)	781
PspN4I	GGNNCC	3 cut(s)	100, 353, 367
PspPI	GGNCC	2 cut(s)	351, 365
PspPPI	RGGWCCY	1 cut(s)	351
PsuI	RGATCY	3 cut(s)	14, 98, 152
PsyI	GACNNNGTC	1 cut(s)	257
RsaI	GTAC	1 cut(s)	800
RsaNI	GTAC	1 cut(s)	799
RseI	CAYNNNNRTG	1 cut(s)	52
SaqAI	TTAA	3 cut(s)	449, 759, 811
Sau3AI	GATC	6 cut(s)	14, 19, 98, 152, 612, 774
Sau96I	GGNCC	2 cut(s)	351, 365
ScrFI	CCNGG	1 cut(s)	783
SduI	GDGCHC	1 cut(s)	288
SfaNI	GCATC	2 cut(s)	137, 610
SfcI	CTRYAG	1 cut(s)	718
SinI	GGWCC	1 cut(s)	351
SmiMI	CAYNNNNRTG	1 cut(s)	52
SmlI	CTYRAG	1 cut(s)	758
SmoI	CTYRAG	1 cut(s)	758
Sse9I	AATT	5 cut(s)	325, 576, 617, 636, 723
SsiI	CCGC	1 cut(s)	715
SspMI	CTAG	6 cut(s)	137, 311, 441, 476, 659, 684
StyD4I	CCNGG	1 cut(s)	781
StyI	CCWWGG	2 cut(s)	347, 475
TaaI	ACNGT	2 cut(s)	125, 499
TasI	AATT	5 cut(s)	325, 576, 617, 636, 723
TfiI	GAWTC	3 cut(s)	218, 334, 663
Tru1I	TTAA	3 cut(s)	449, 759, 811
Tru9I	TTAA	3 cut(s)	449, 759, 811
TscAI	CASTG	1 cut(s)	502
TseFI	GTSAC	2 cut(s)	119, 259
Tsp45I	GTSAC	2 cut(s)	119, 259
TspDTI	ATGAA	9 cut(s)	162, 207, 231, 306, 395, 543, 549, 570, 759
TspRI	CASTG	1 cut(s)	502
Tth111I	GACNNNGTC	1 cut(s)	257
Vha464I	CTTAAG	1 cut(s)	758
VpaK11BI	GGWCC	1 cut(s)	351
XagI	CCTNNNNNAGG	1 cut(s)	479
XapI	RAATTY	1 cut(s)	636
XmaJI	CCTAGG	1 cut(s)	475
XspI	CTAG	6 cut(s)	137, 311, 441, 476, 659, 684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.