Rh4BG043700

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000529
Physical Location & Seq
Forward (+)
7900642 .. 7906198
5557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG043700.1

Sequence Viewer

Length: 738 bp
ATGGTGTTGGATTCATTGAGGGAGCCAACCGTGCTGCTGGGGGTGAAGCGTAAGGCTATGGAGGCTTTGGATCGAGTAGGCAAGTGGTATGTTTCAAACCCAGAACCTTTGCTCCAGGGTACAAGGTCCCAATCAAATGGCCCCAGAGCAGAGATGAAGTATGGAAAGCAAATATACCTCATACTCACCTTGCACATGAGAAATCCGACCAGAACTGGATGGTTGAAAAAGGTGACAAGATTAGTTTTCCTGGGGGAGGCACGCATTTTCACTATGGAGCTGATAAGTATATTGCTTCAATTGCAAATGTTACGTACAGTTTTTGACGTTGGCTGTGGAGTTGCAAGTTTTGGAGGCTATCTTCTGTCATCTGATATTATAACAATGTCCTTAGCACCCAATGATGTGCATCAAAATCAAATCCAATTTGCTTTGGAAAGAGGAATTCCAGCATATCTTGGTGTTCTAGGGACCAAAAGGCTTCCTTACCCAAGCAGATCTTTTGAACTTGCTCACTGTTCCCGTTGTAGAATTGATTGGCTCCAAAGAGATGGGATCCATCTTCTTGAGCTAGAGCCTGAGGAGGCTACTTTGCGTACTCATCTCCAGAAGCATATGCACAGGAGAGGAAGATCTCAAAATATGGAAAGAGATGAGTGCCCTTGTGGAACGCATGTGTTGGAGAATAGCTGCAAAAAGGAACCAAACTGTCATTTGGCAGAAACCACTAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

245

Amino Acids

27.98

Weight (kDa)

9.23

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 98 - 192 1e-48 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AclWI GGATC 3 cut(s) 78, 550, 563
AcsI RAATTY 1 cut(s) 444
AfaI GTAC 3 cut(s) 121, 316, 598
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 4 cut(s) 96, 226, 299, 506
AjnI CCWGG 2 cut(s) 114, 249
AloI GAACNNNNNNTCC 2 cut(s) 96, 128
AluBI AGCT 3 cut(s) 280, 571, 690
AluI AGCT 3 cut(s) 280, 571, 690
AlwI GGATC 3 cut(s) 78, 550, 563
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 34, 690
ApoI RAATTY 1 cut(s) 444
AspS9I GGNCC 3 cut(s) 126, 140, 471
AsuHPI GGTGA 3 cut(s) 55, 178, 244
AvaII GGWCC 2 cut(s) 126, 471
AxyI CCTNAGG 1 cut(s) 579
BaeGI GKGCMC 1 cut(s) 662
BamHI GGATCC 1 cut(s) 555
BbvI GCAGC 2 cut(s) 21, 677
BccI CCATC 3 cut(s) 213, 545, 567
BciT130I CCWGG 2 cut(s) 116, 251
BfaI CTAG 2 cut(s) 467, 572
BglII AGATCT 2 cut(s) 497, 632
BisI GCNGC 2 cut(s) 35, 691
BlsI GCNGC 2 cut(s) 36, 692
Bme1390I CCNGG 2 cut(s) 116, 251
Bme18I GGWCC 2 cut(s) 126, 471
BmgT120I GGNCC 3 cut(s) 126, 140, 471
BmiI GGNNCC 7 cut(s) 24, 128, 142, 472, 542, 557, 702
BmrFI CCNGG 2 cut(s) 116, 251
BmsI GCATC 1 cut(s) 418
BpmI CTGGAG 2 cut(s) 98, 590
Bpu10I CCTNAGC 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 587
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 115, 250
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 1 cut(s) 220
Bse21I CCTNAGG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 2 cut(s) 116, 251
BseDI CCNNGG 2 cut(s) 115, 250
BseGI GGATG 1 cut(s) 224
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 596
BseSI GKGCMC 1 cut(s) 662
BseXI GCAGC 2 cut(s) 21, 677
BseYI CCCAGC 1 cut(s) 37
BshFI GGCC 1 cut(s) 141
BslFI GGGAC 2 cut(s) 112, 484
BslI CCNNNNNNNGG 1 cut(s) 256
BsmFI GGGAC 2 cut(s) 112, 484
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 662
Bsp143I GATC 4 cut(s) 70, 497, 555, 632
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 571
BspLI GGNNCC 7 cut(s) 24, 128, 142, 472, 542, 557, 702
BspPI GGATC 3 cut(s) 78, 550, 563
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 115, 250
BssMI GATC 4 cut(s) 70, 497, 555, 632
Bst2UI CCWGG 2 cut(s) 116, 251
Bst4CI ACNGT 4 cut(s) 31, 319, 518, 710
BstBAI YACGTR 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 2 cut(s) 391, 579
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 1 cut(s) 224
BstKTI GATC 4 cut(s) 73, 500, 558, 635
BstMBI GATC 4 cut(s) 70, 497, 555, 632
BstMWI GCNNNNNNNGC 3 cut(s) 31, 62, 301
BstNI CCWGG 2 cut(s) 116, 251
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 2 cut(s) 114, 249
BstSLI GKGCMC 1 cut(s) 662
BstSNI TACGTA 1 cut(s) 314
BstV1I GCAGC 2 cut(s) 21, 677
BstX2I RGATCY 3 cut(s) 497, 555, 632
BstXI CCANNNNNNTGG 2 cut(s) 137, 551
BstYI RGATCY 3 cut(s) 497, 555, 632
Bsu36I CCTNAGG 1 cut(s) 579
BsuRI GGCC 1 cut(s) 141
BtsCI GGATG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 514
Cac8I GCNNGC 1 cut(s) 262
Cfr13I GGNCC 3 cut(s) 126, 140, 471
Csp6I GTAC 3 cut(s) 120, 315, 597
CviAII CATG 2 cut(s) 196, 674
CviQI GTAC 3 cut(s) 120, 315, 597
DdeI CTNAG 2 cut(s) 391, 579
DpnI GATC 4 cut(s) 72, 499, 557, 634
DpnII GATC 4 cut(s) 70, 497, 555, 632
Eco105I TACGTA 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 126, 471
Eco81I CCTNAGG 1 cut(s) 579
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 2 cut(s) 114, 249
FaeI CATG 2 cut(s) 199, 677
FalI AAGNNNNNCTT 4 cut(s) 469, 501, 484, 516
FaqI GGGAC 2 cut(s) 112, 484
FatI CATG 2 cut(s) 195, 673
FauNDI CATATG 1 cut(s) 615
Fnu4HI GCNGC 2 cut(s) 35, 691
FokI GGATG 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 35, 691
FspBI CTAG 2 cut(s) 467, 572
GluI GCNGC 2 cut(s) 35, 691
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 2 cut(s) 98, 590
HaeIII GGCC 1 cut(s) 141
Hin1II CATG 2 cut(s) 199, 677
HinfI GANTC 1 cut(s) 11
HphI GGTGA 3 cut(s) 55, 178, 244
Hpy188I TCNGA 2 cut(s) 207, 373
Hpy188III TCNNGA 2 cut(s) 566, 607
HpyCH4III ACNGT 4 cut(s) 31, 319, 518, 710
HpyCH4IV ACGT 2 cut(s) 313, 327
HpyCH4V TGCA 6 cut(s) 193, 304, 344, 409, 619, 693
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 62, 301
HpyF3I CTNAG 2 cut(s) 391, 579
HpySE526I ACGT 2 cut(s) 313, 327
Hsp92II CATG 2 cut(s) 199, 677
Kzo9I GATC 4 cut(s) 70, 497, 555, 632
LmnI GCTCC 4 cut(s) 22, 117, 277, 546
Lsp1109I GCAGC 2 cut(s) 21, 677
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 467, 572
MaeII ACGT 2 cut(s) 313, 327
MaeIII GTNAC 2 cut(s) 232, 309
MalI GATC 4 cut(s) 72, 499, 557, 634
MboI GATC 4 cut(s) 70, 497, 555, 632
MboII GAAGA 3 cut(s) 353, 554, 642
MfeI CAATTG 1 cut(s) 299
MflI RGATCY 3 cut(s) 497, 555, 632
MhlI GDGCHC 1 cut(s) 662
MluCI AATT 4 cut(s) 299, 425, 444, 531
MmeI TCCRAC 2 cut(s) 230, 660
MnlI CCTC 9 cut(s) 12, 55, 188, 250, 347, 434, 574, 577, 620
MspR9I CCNGG 2 cut(s) 116, 251
MunI CAATTG 1 cut(s) 299
MvaI CCWGG 2 cut(s) 116, 251
MwoI GCNNNNNNNGC 3 cut(s) 31, 62, 301
NdeI CATATG 1 cut(s) 615
NdeII GATC 4 cut(s) 70, 497, 555, 632
NlaIII CATG 2 cut(s) 199, 677
NlaIV GGNNCC 7 cut(s) 24, 128, 142, 472, 542, 557, 702
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 1 cut(s) 677
PfeI GAWTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 36, 692
Ppu21I YACGTR 1 cut(s) 314
PpuMI RGGWCCY 1 cut(s) 126
PsiI TTATAA 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 114, 249
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 2 cut(s) 114, 249
PspN4I GGNNCC 7 cut(s) 24, 128, 142, 472, 542, 557, 702
PspPI GGNCC 3 cut(s) 126, 140, 471
PspPPI RGGWCCY 1 cut(s) 126
PsuI RGATCY 3 cut(s) 497, 555, 632
RsaI GTAC 3 cut(s) 121, 316, 598
RsaNI GTAC 3 cut(s) 120, 315, 597
SatI GCNGC 2 cut(s) 35, 691
Sau3AI GATC 4 cut(s) 70, 497, 555, 632
Sau96I GGNCC 3 cut(s) 126, 140, 471
ScrFI CCNGG 2 cut(s) 116, 251
SduI GDGCHC 1 cut(s) 662
SfaNI GCATC 1 cut(s) 418
SinI GGWCC 2 cut(s) 126, 471
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SnaBI TACGTA 1 cut(s) 314
Sse9I AATT 4 cut(s) 299, 425, 444, 531
SspMI CTAG 2 cut(s) 467, 572
StyD4I CCNGG 2 cut(s) 114, 249
TaaI ACNGT 4 cut(s) 31, 319, 518, 710
TaiI ACGT 2 cut(s) 316, 330
TaqI TCGA 1 cut(s) 73
TasI AATT 4 cut(s) 299, 425, 444, 531
TfiI GAWTC 1 cut(s) 11
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 2 cut(s) 34, 690
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 170
TspRI CASTG 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 126, 471
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 1 cut(s) 444
XceI RCATGY 1 cut(s) 677
XspI CTAG 2 cut(s) 467, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.