Rh4AG047800

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000529
Physical Location & Seq
Forward (+)
10039381 .. 10042572
3192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG047800.1

Sequence Viewer

Length: 1182 bp
ATGAAGTATGGAAAGCAAATATACCTCATACTCACCTTGCACATGAGAAATCCGACCAGAACTGGATGGTTGAAAAGGGTGACAAGATTAGTTTTCCTGGGGGAGGCACACATTTTCACTATGGAGCTGATAAGTATATTGCTTCAATTGCAAATGTTACGTACAGTTTTTGACGTTGGCTGTGGAGTTGCAAGTTTTGGAGGCTATCTTCTGTCATCTGATATTATAACAATGTCCTTAGCGGCCAATGATGTGCATCAAAATCAAATCCAATTTGCTTTGGAAAGAGGAATTCCAGCATATCTTGGTGTTCTGGGGACCAAAAGTCTTCCTTACCCAAGCAGATCTTTTGAACTTGCTCACTGTTCCCGTTGTAGAATTGATTGGCTCCAAAGAGATGGGATCCTTCTTCTTGAGCTAGATAGGTTGCTCAGGCCAGGAGGCTACTTTGCGTACTCATCTCCAGAAGCATATGCACAGGATGAGGAAGATCTCAAAATATGGAAAGAGATGAGTGCCCTTGTGGAACGCATGTGTTGGAGAATAGCTGCAAAAAGGAACCAAACTGTCATTTGGCAGAAACCACTAACAAATGACTGTTATATGCAAAGAGAACCTGGCACTCAACCTCCTCTCTGCCGATCTGATGATGATCCAGATGCAATCTGGGCTGCGCCAATGGAAGCTTGCATCTCACCGTACTCTGATCATGACCATAGAGAAAAGGGGAGTGGATTGGCTCCCTGGCCAGCTAGATTGACAACTCCTCCTCGACTTGCTGGTTTTGGCTATTCAAATGAAATGTTTGAAAAGGATATGGAACTTTGGCGGCATCGAGTTGAGAATTATTGGAATCTCTTGAGTCCAAAGATTGAATCAAACACTCTGAGGAATGTGATGGATATGAAAGCACACATGGGATCATTTGCAGCTGCTCTGAAGGACAAGGATGTTTGGGTGATGAATGTCGTCCCTGAAGATGGACCAAACACACTAAAGCTGATATACGATAGAGGCCTCATAGGCAGTATTCACAGCTGGTGTGAAGCCTATTCAACATACCCCGTACTTACGATTTACTCCATGCTTGGACTGTCTTCTCTGACTTGGAAAAGAAAGCACTGCATTCTGAGGATCTGTTACTTGAGATGGATCGCATACTCAGGCCAACTGGATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

393

Amino Acids

45.17

Weight (kDa)

7.98

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 46 - 368 9.8e-142 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 58 - 151 6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 227, 1180
AciI CCGC 2 cut(s) 242, 829
AclWI GGATC 6 cut(s) 397, 410, 647, 928, 1142, 1160
AcoI YGGCCR 2 cut(s) 243, 746
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 2 cut(s) 959, 996
AfaI GTAC 4 cut(s) 163, 455, 701, 1068
AfiI CCNNNNNNNGG 2 cut(s) 103, 980
AgsI TTSAA 7 cut(s) 73, 146, 353, 795, 809, 875, 1056
AhdI GACNNNNNGTC 1 cut(s) 324
AjnI CCWGG 4 cut(s) 96, 436, 616, 743
AluBI AGCT 8 cut(s) 127, 418, 548, 686, 752, 932, 1000, 1038
AluI AGCT 8 cut(s) 127, 418, 548, 686, 752, 932, 1000, 1038
AlwI GGATC 6 cut(s) 397, 410, 647, 928, 1142, 1160
AoxI GGCC 5 cut(s) 243, 434, 746, 1015, 1165
ApeKI GCWGC 4 cut(s) 548, 671, 929, 932
ApoI RAATTY 1 cut(s) 291
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 2 cut(s) 318, 983
AsuHPI GGTGA 4 cut(s) 25, 91, 687, 970
AvaII GGWCC 2 cut(s) 318, 983
BaeGI GKGCMC 1 cut(s) 520
BalI TGGCCA 1 cut(s) 748
BamHI GGATCC 1 cut(s) 402
BbsI GAAGAC 2 cut(s) 320, 1089
BbvI GCAGC 4 cut(s) 535, 658, 919, 941
BccI CCATC 5 cut(s) 60, 392, 892, 974, 1143
BciT130I CCWGG 4 cut(s) 98, 438, 618, 745
BclI TGATCA 1 cut(s) 706
BfaI CTAG 2 cut(s) 419, 753
BglI GCCNNNNNGGC 1 cut(s) 1023
BglII AGATCT 2 cut(s) 344, 490
BisI GCNGC 6 cut(s) 243, 549, 672, 830, 930, 933
BlsI GCNGC 6 cut(s) 244, 550, 673, 831, 931, 934
Bme1390I CCNGG 4 cut(s) 98, 438, 618, 745
Bme18I GGWCC 2 cut(s) 318, 983
BmeRI GACNNNNNGTC 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 318, 983
BmiI GGNNCC 5 cut(s) 319, 389, 404, 560, 741
BmrFI CCNGG 4 cut(s) 98, 438, 618, 745
BmsI GCATC 4 cut(s) 265, 649, 699, 841
BpiI GAAGAC 2 cut(s) 320, 1089
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 2 cut(s) 238, 431
BpuEI CTTGAG 3 cut(s) 434, 880, 1165
BsaAI YACGTR 1 cut(s) 161
BsaBI GATNNNNATC 2 cut(s) 255, 651
BsaJI CCNNGG 2 cut(s) 97, 743
BsaXI ACNNNNNCTCC 4 cut(s) 613, 643, 751, 781
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 980
Bse1I ACTGG 2 cut(s) 67, 1176
Bse8I GATNNNNATC 2 cut(s) 255, 651
BseBI CCWGG 4 cut(s) 98, 438, 618, 745
BseDI CCNNGG 2 cut(s) 97, 743
BseGI GGATG 3 cut(s) 71, 487, 955
BseJI GATNNNNATC 2 cut(s) 255, 651
BseLI CCNNNNNNNGG 2 cut(s) 103, 980
BseMII CTCAG 4 cut(s) 445, 878, 1121, 1176
BseNI ACTGG 2 cut(s) 67, 1176
BseRI GAGGAG 3 cut(s) 621, 756, 759
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 4 cut(s) 535, 658, 919, 941
BshFI GGCC 5 cut(s) 245, 436, 748, 1017, 1167
BslFI GGGAC 2 cut(s) 331, 956
BslI CCNNNNNNNGG 2 cut(s) 103, 980
BsmFI GGGAC 2 cut(s) 331, 956
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 5 cut(s) 245, 436, 748, 1017, 1167
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
BspACI CCGC 2 cut(s) 242, 829
BspANI GGCC 5 cut(s) 245, 436, 748, 1017, 1167
BspCNI CTCAG 4 cut(s) 444, 879, 1122, 1175
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 5 cut(s) 319, 389, 404, 560, 741
BspPI GGATC 6 cut(s) 397, 410, 647, 928, 1142, 1160
BsrI ACTGG 2 cut(s) 67, 1176
BssECI CCNNGG 2 cut(s) 97, 743
BssMI GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
Bst2UI CCWGG 4 cut(s) 98, 438, 618, 745
Bst4CI ACNGT 6 cut(s) 166, 365, 568, 599, 699, 1095
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 688, 750
BstDEI CTNAG 5 cut(s) 238, 431, 887, 1130, 1162
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 3 cut(s) 71, 487, 955
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 9 cut(s) 347, 405, 493, 644, 655, 709, 923, 1137, 1155
BstMBI GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
BstMWI GCNNNNNNNGC 3 cut(s) 148, 668, 1023
BstNI CCWGG 4 cut(s) 98, 438, 618, 745
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 4 cut(s) 96, 436, 616, 743
BstSLI GKGCMC 1 cut(s) 520
BstSNI TACGTA 1 cut(s) 161
BstV1I GCAGC 4 cut(s) 535, 658, 919, 941
BstV2I GAAGAC 2 cut(s) 320, 1089
BstX2I RGATCY 4 cut(s) 344, 402, 490, 1134
BstXI CCANNNNNNTGG 1 cut(s) 398
BstYI RGATCY 4 cut(s) 344, 402, 490, 1134
BsuRI GGCC 5 cut(s) 245, 436, 748, 1017, 1167
BtsCI GGATG 3 cut(s) 71, 487, 955
BtsI GCAGTG 1 cut(s) 1120
BtsIMutI CAGTG 2 cut(s) 361, 1120
Cac8I GCNNGC 2 cut(s) 688, 750
CciI TCATGA 1 cut(s) 709
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 2 cut(s) 318, 983
Csp6I GTAC 4 cut(s) 162, 454, 700, 1067
CviAII CATG 5 cut(s) 43, 532, 710, 916, 1084
CviQI GTAC 4 cut(s) 162, 454, 700, 1067
DdeI CTNAG 5 cut(s) 238, 431, 887, 1130, 1162
DpnI GATC 9 cut(s) 346, 404, 492, 643, 654, 708, 922, 1136, 1154
DpnII GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
DriI GACNNNNNGTC 1 cut(s) 324
EaeI YGGCCR 2 cut(s) 243, 746
Eam1105I GACNNNNNGTC 1 cut(s) 324
Eco105I TACGTA 1 cut(s) 161
Eco147I AGGCCT 1 cut(s) 1017
Eco47I GGWCC 2 cut(s) 318, 983
Eco57I CTGAAG 2 cut(s) 959, 996
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 4 cut(s) 96, 436, 616, 743
FaeI CATG 5 cut(s) 46, 535, 713, 919, 1087
FalI AAGNNNNNCTT 4 cut(s) 316, 348, 331, 363
FaqI GGGAC 2 cut(s) 331, 956
FatI CATG 5 cut(s) 42, 531, 709, 915, 1083
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 6 cut(s) 243, 549, 672, 830, 930, 933
FokI GGATG 3 cut(s) 78, 494, 962
Fsp4HI GCNGC 6 cut(s) 243, 549, 672, 830, 930, 933
FspBI CTAG 2 cut(s) 419, 753
GlaI GCGC 1 cut(s) 675
GluI GCNGC 6 cut(s) 243, 549, 672, 830, 930, 933
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 5 cut(s) 245, 436, 748, 1017, 1167
HhaI GCGC 1 cut(s) 676
Hin1II CATG 5 cut(s) 46, 535, 713, 919, 1087
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 3 cut(s) 853, 862, 875
HphI GGTGA 4 cut(s) 25, 91, 687, 970
Hpy188I TCNGA 8 cut(s) 54, 220, 646, 706, 888, 939, 1104, 1131
Hpy188III TCNNGA 5 cut(s) 413, 464, 656, 710, 859
HpyAV CCTTC 2 cut(s) 416, 934
HpyCH4III ACNGT 6 cut(s) 166, 365, 568, 599, 699, 1095
HpyCH4IV ACGT 2 cut(s) 160, 174
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 668, 1023
HpyF3I CTNAG 5 cut(s) 238, 431, 887, 1130, 1162
HpySE526I ACGT 2 cut(s) 160, 174
Hsp92II CATG 5 cut(s) 46, 535, 713, 919, 1087
HspAI GCGC 1 cut(s) 674
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
LmnI GCTCC 3 cut(s) 124, 393, 745
Lsp1109I GCAGC 4 cut(s) 535, 658, 919, 941
LweI GCATC 4 cut(s) 265, 649, 699, 841
MaeI CTAG 2 cut(s) 419, 753
MaeII ACGT 2 cut(s) 160, 174
MaeIII GTNAC 3 cut(s) 79, 156, 1139
MalI GATC 9 cut(s) 346, 404, 492, 643, 654, 708, 922, 1136, 1154
MboI GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
MboII GAAGA 6 cut(s) 200, 320, 401, 500, 989, 1089
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 4 cut(s) 344, 402, 490, 1134
MhlI GDGCHC 1 cut(s) 520
MlsI TGGCCA 1 cut(s) 748
MluCI AATT 5 cut(s) 146, 272, 291, 378, 844
MluNI TGGCCA 1 cut(s) 748
MlyI GAGTC 1 cut(s) 871
MmeI TCCRAC 2 cut(s) 77, 518
Mox20I TGGCCA 1 cut(s) 748
MscI TGGCCA 1 cut(s) 748
Msp20I TGGCCA 1 cut(s) 748
MspA1I CMGCKG 2 cut(s) 932, 1038
MspR9I CCNGG 4 cut(s) 98, 438, 618, 745
MunI CAATTG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 4 cut(s) 98, 438, 618, 745
MwoI GCNNNNNNNGC 3 cut(s) 148, 668, 1023
NdeI CATATG 1 cut(s) 472
NdeII GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
NlaIII CATG 5 cut(s) 46, 535, 713, 919, 1087
NlaIV GGNNCC 5 cut(s) 319, 389, 404, 560, 741
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 1 cut(s) 535
PagI TCATGA 1 cut(s) 709
PceI AGGCCT 1 cut(s) 1017
PctI GAATGC 1 cut(s) 1125
PfeI GAWTC 2 cut(s) 853, 875
PkrI GCNGC 6 cut(s) 244, 550, 673, 831, 931, 934
PleI GAGTC 1 cut(s) 870
PpsI GAGTC 1 cut(s) 870
Ppu21I YACGTR 1 cut(s) 161
PsiI TTATAA 2 cut(s) 227, 1180
Psp6I CCWGG 4 cut(s) 96, 436, 616, 743
PspGI CCWGG 4 cut(s) 96, 436, 616, 743
PspN4I GGNNCC 5 cut(s) 319, 389, 404, 560, 741
PspPI GGNCC 2 cut(s) 318, 983
PsuI RGATCY 4 cut(s) 344, 402, 490, 1134
PvuII CAGCTG 2 cut(s) 932, 1038
RsaI GTAC 4 cut(s) 163, 455, 701, 1068
RsaNI GTAC 4 cut(s) 162, 454, 700, 1067
SatI GCNGC 6 cut(s) 243, 549, 672, 830, 930, 933
Sau3AI GATC 9 cut(s) 344, 402, 490, 641, 652, 706, 920, 1134, 1152
Sau96I GGNCC 2 cut(s) 318, 983
SchI GAGTC 1 cut(s) 871
ScrFI CCNGG 4 cut(s) 98, 438, 618, 745
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 4 cut(s) 265, 649, 699, 841
SinI GGWCC 2 cut(s) 318, 983
SmlI CTYRAG 3 cut(s) 413, 859, 1144
SmoI CTYRAG 3 cut(s) 413, 859, 1144
SnaBI TACGTA 1 cut(s) 161
Sse9I AATT 5 cut(s) 146, 272, 291, 378, 844
SseBI AGGCCT 1 cut(s) 1017
SsiI CCGC 2 cut(s) 242, 829
SspMI CTAG 2 cut(s) 419, 753
StuI AGGCCT 1 cut(s) 1017
StyD4I CCNGG 4 cut(s) 96, 436, 616, 743
TaaI ACNGT 6 cut(s) 166, 365, 568, 599, 699, 1095
TaiI ACGT 2 cut(s) 163, 177
TaqI TCGA 2 cut(s) 772, 835
TasI AATT 5 cut(s) 146, 272, 291, 378, 844
TauI GCSGC 2 cut(s) 245, 832
TfiI GAWTC 2 cut(s) 853, 875
TscAI CASTG 2 cut(s) 368, 1127
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 4 cut(s) 548, 671, 929, 932
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 4 cut(s) 17, 813, 920, 977
TspRI CASTG 2 cut(s) 368, 1127
VpaK11BI GGWCC 2 cut(s) 318, 983
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 291
XceI RCATGY 1 cut(s) 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 663
XspI CTAG 2 cut(s) 419, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.