Rh4CG050700

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000529
Physical Location & Seq
Forward (+)
10132297 .. 10134219
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG050700.1

Sequence Viewer

Length: 507 bp
ATGACTGTTATATGCAAAGAGAACCTGGCACTCAACGTCCTCTCTGCCGATCTGATGATGATCCAGATGCAATCTGGGGTGTGCCAACGGAAGCTTGCATCTCACCGTACTCTGATCGCAAATGACCATAGAGAAAAGGGGAGTGGATTGGCTCCCTGGCCAGCTAGATTGACAACTCCTCCTCCTCGACTTGCTGATTTTGGCTATTCAAATGAAATGTTTGAAAAGGATATGGAACTTTGGCGGCATCGAGTTGAGAATTATTGGAATCTCTTGAGTCCAAAGATTGAATCAAACACTCTGAGGAATGTGATGGATATGAAAGCACACATGGGATCATTTGCAGCTGCTCTGAAGGACAAGGATGTTTGGGTGATGAATGTCGTCCCTGAAGATGGACCAAACACACTAAAGCTGATATACGATAGAGGCCTCACAGCTGGTGTGAAGCCTATTCAACATACCCCGTACTTACGATTTACTCCATGCTTGGACTGTCTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

168

Amino Acids

19.16

Weight (kDa)

8.36

Isoelectric Point (pI)

24.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 40 - 146 3.1e-37 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 244
AclWI GGATC 2 cut(s) 55, 343
AcoI YGGCCR 1 cut(s) 158
AcuI CTGAAG 2 cut(s) 374, 411
AfaI GTAC 2 cut(s) 109, 470
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 4 cut(s) 210, 224, 290, 458
AjnI CCWGG 2 cut(s) 24, 155
AluBI AGCT 5 cut(s) 94, 164, 347, 415, 440
AluI AGCT 5 cut(s) 94, 164, 347, 415, 440
AlwI GGATC 2 cut(s) 55, 343
AoxI GGCC 2 cut(s) 158, 430
ApeKI GCWGC 2 cut(s) 344, 347
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 95, 385
AvaII GGWCC 1 cut(s) 398
BalI TGGCCA 1 cut(s) 160
BbvI GCAGC 2 cut(s) 334, 356
BccI CCATC 2 cut(s) 307, 389
BciT130I CCWGG 2 cut(s) 26, 157
BfaI CTAG 1 cut(s) 165
BisI GCNGC 3 cut(s) 245, 345, 348
BlsI GCNGC 3 cut(s) 246, 346, 349
Bme1390I CCNGG 2 cut(s) 26, 157
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 26, 157
BmsI GCATC 3 cut(s) 57, 107, 256
BpuEI CTTGAG 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 4 cut(s) 163, 166, 193, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 2 cut(s) 26, 157
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 370
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 293
BseRI GAGGAG 3 cut(s) 168, 171, 174
BseXI GCAGC 2 cut(s) 334, 356
BshFI GGCC 2 cut(s) 160, 432
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 2 cut(s) 160, 432
Bsp143I GATC 4 cut(s) 49, 60, 114, 335
BspACI CCGC 1 cut(s) 244
BspANI GGCC 2 cut(s) 160, 432
BspCNI CTCAG 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 2 cut(s) 55, 343
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 4 cut(s) 49, 60, 114, 335
Bst2UI CCWGG 2 cut(s) 26, 157
Bst4CI ACNGT 3 cut(s) 7, 107, 497
BstC8I GCNNGC 2 cut(s) 96, 162
BstDEI CTNAG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 370
BstKTI GATC 4 cut(s) 52, 63, 117, 338
BstMBI GATC 4 cut(s) 49, 60, 114, 335
BstNI CCWGG 2 cut(s) 26, 157
BstSCI CCNGG 2 cut(s) 24, 155
BstV1I GCAGC 2 cut(s) 334, 356
BsuRI GGCC 2 cut(s) 160, 432
BtsCI GGATG 1 cut(s) 370
Cac8I GCNNGC 2 cut(s) 96, 162
Cfr13I GGNCC 1 cut(s) 398
Csp6I GTAC 2 cut(s) 108, 469
CviAII CATG 2 cut(s) 331, 486
CviQI GTAC 2 cut(s) 108, 469
DdeI CTNAG 1 cut(s) 302
DpnI GATC 4 cut(s) 51, 62, 116, 337
DpnII GATC 4 cut(s) 49, 60, 114, 335
EaeI YGGCCR 1 cut(s) 158
Eco147I AGGCCT 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 374, 411
EcoRII CCWGG 2 cut(s) 24, 155
FaeI CATG 2 cut(s) 334, 489
FaiI YATR 9 cut(s) 11, 13, 129, 233, 320, 332, 421, 462, 487
FaqI GGGAC 1 cut(s) 371
FatI CATG 2 cut(s) 330, 485
Fnu4HI GCNGC 3 cut(s) 245, 345, 348
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 3 cut(s) 245, 345, 348
FspBI CTAG 1 cut(s) 165
GluI GCNGC 3 cut(s) 245, 345, 348
HaeIII GGCC 2 cut(s) 160, 432
Hin1II CATG 2 cut(s) 334, 489
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 268, 277, 290
HphI GGTGA 2 cut(s) 95, 385
Hpy188I TCNGA 5 cut(s) 54, 114, 303, 354, 506
Hpy188III TCNNGA 2 cut(s) 64, 274
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 3 cut(s) 7, 107, 497
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 4 cut(s) 15, 70, 98, 344
HpyF3I CTNAG 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 2 cut(s) 334, 489
Kzo9I GATC 4 cut(s) 49, 60, 114, 335
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 9 cut(s) 11, 38, 60, 77, 142, 169, 174, 402, 426
Lsp1109I GCAGC 2 cut(s) 334, 356
LweI GCATC 3 cut(s) 57, 107, 256
MaeI CTAG 1 cut(s) 165
MaeII ACGT 1 cut(s) 36
MalI GATC 4 cut(s) 51, 62, 116, 337
MboI GATC 4 cut(s) 49, 60, 114, 335
MboII GAAGA 1 cut(s) 404
MlsI TGGCCA 1 cut(s) 160
MluCI AATT 1 cut(s) 259
MluNI TGGCCA 1 cut(s) 160
MlyI GAGTC 1 cut(s) 286
MnlI CCTC 7 cut(s) 50, 189, 192, 195, 297, 422, 443
Mox20I TGGCCA 1 cut(s) 160
MscI TGGCCA 1 cut(s) 160
Msp20I TGGCCA 1 cut(s) 160
MspA1I CMGCKG 2 cut(s) 347, 440
MspR9I CCNGG 2 cut(s) 26, 157
MvaI CCWGG 2 cut(s) 26, 157
NdeII GATC 4 cut(s) 49, 60, 114, 335
NlaIII CATG 2 cut(s) 334, 489
NlaIV GGNNCC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 432
PfeI GAWTC 2 cut(s) 268, 290
PkrI GCNGC 3 cut(s) 246, 346, 349
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 24, 155
PspGI CCWGG 2 cut(s) 24, 155
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 398
PvuII CAGCTG 2 cut(s) 347, 440
RsaI GTAC 2 cut(s) 109, 470
RsaNI GTAC 2 cut(s) 108, 469
SatI GCNGC 3 cut(s) 245, 345, 348
Sau3AI GATC 4 cut(s) 49, 60, 114, 335
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 2 cut(s) 26, 157
SetI ASST 7 cut(s) 27, 39, 96, 166, 349, 417, 442
SfaNI GCATC 3 cut(s) 57, 107, 256
SinI GGWCC 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 1 cut(s) 259
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 1 cut(s) 244
SspMI CTAG 1 cut(s) 165
StuI AGGCCT 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 24, 155
TaaI ACNGT 3 cut(s) 7, 107, 497
TaiI ACGT 1 cut(s) 39
TaqI TCGA 2 cut(s) 187, 250
TasI AATT 1 cut(s) 259
TauI GCSGC 1 cut(s) 247
TfiI GAWTC 2 cut(s) 268, 290
TseI GCWGC 2 cut(s) 344, 347
TspDTI ATGAA 3 cut(s) 228, 335, 392
TspGWI ACGGA 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.