Rw4G003770

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000529
Physical Location & Seq
Forward (+)
7308795 .. 7310905
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003770.1

Sequence Viewer

Length: 681 bp
ATGGAAAGAGATGAGTGCCCTTGTGGAATGCATGTGTTGGAGAATAGCTGCAAAAAGGAACCAACTGTCATTTGGCAGAAACCACTAACAAATGACTGTTATATGCAAAGAGAACCTGGCACTCAACGTCCTCTCTGCCGATCTGATGATGATCCAGATGCAATCTGGGGTGTGCCAACGGAAGCTTGCATCTCACCGTACTCTGATCATGACCATAGAGAAAAGGGGAGTGGATTGGCTCCCTGGCCAGCTAGATTGACAACTGCTCCTCGACTTGCTGGTTTTGGCTATTCAAATGAAATGTTTGAAAAGGATATGGAACTTTGGCGGCATCGAGTTGAGAATTATTGGAATCTCTTGAGTCCAAAGATTGAATCAAACACTCTGAGGAATGTGATGGATATGAAGGCTCACATGGGATCATTTGCAGCTGCTCTGAAGGACAAGGATGTTTGGGTGATGAATGTCGTCCCTGAAGATGGACCAAACACACTAAAGCTGATATACGATAGAGGCCTCATAGGCAGTATTCACAGCTGGTGTGAAGCCTATTCAACATACCCCGTACTTACGATTTACTCCATGCTTGGACTGTCTTCTCTGACTTGGAAAAGAAAGCACTGCATTCTGAGGATCTGTTACTTGAGATGGATCGCATACTCAGGCCAACTGGATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

226

Amino Acids

26.05

Weight (kDa)

6.17

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 17 - 201 3.5e-65 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04430 AT1G04430 AT1G04430 AT3G23300 AT3G23300 AT4G14360 AT4G14360
fragaria_vesca FvH4_2g21260 FvH4_2g21260 FvH4_2g21260 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770 FvH4_4g03770
malus_domestica MD05G1195000.v1.1 MD10G1181600.v1.1 MD10G1182100.v1.1 MD13G1215000.v1.1
prunus_persica Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.1G040400_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1 Prupe.8G221700_v2.0.a1
pyrus_communis pycom05g18230 pycom10g15760 pycom10g15770 pycom111g01800
rosa_chinensis RchiOBHm_Chr4g0393501 RchiOBHm_Chr4g0393511 RchiOBHm_Chr4g0393631 RchiOBHm_Chr6g0287841
rosa_laevigata RLG00000009742 RLG00000012463
rosa_multiflora Rmu_sc0001531.1_g000028 Rmu_sc0003877.1_g000004 Rmu_sc0007724.1_g000003 Rmu_sc0008931.1_g000001 Rmu_sc0015036.1_g000004 Rmu_sc0021463.1_g000002 Rmu_sc0023299.1_g000001 Rmu_sc0023300.1_g000001 Rmu_sc0036005.1_g000001
rosa_roxburghii Rroxscaffold_5G00338440 Rroxscaffold_5G00338470 Rroxscaffold_5G00338730 Rroxscaffold_5G00338790 Rroxscaffold_7G00180730 Rroxscaffold_7G00180820
rosa_rugosa Rorug03G0372400 Rorug03G0372500 Rorug03G0372600 Rorug04G0000100 Rorug04G0000200 Rorug04G0000300 Rorug04G0000400 Rorug04G0000500 Rorug04G0000600.1 Rorug04G0000700 Rorug04G0000800 Rorug04G0000900 Rorug06G0192300 Rorug06G0192400 Rorug06G0192500
rosa_samantha Rh4AG047800 Rh4AG048100 Rh4BG043700 Rh4BG044600 Rh4CG050500 Rh4CG050600 Rh4CG050700 Rh4CG051400 Rh4DG045000 Rh4DG045300 Rh6AG302000 Rh6BG307900 Rh6CG301600 Rh6CG315800 Rh6DG300500
rosa_wichuraiana Rw4G003770 Rw4G003800 Rw6G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 679
AciI CCGC 1 cut(s) 328
AclWI GGATC 4 cut(s) 146, 427, 641, 659
AcoI YGGCCR 1 cut(s) 245
AcuI CTGAAG 2 cut(s) 458, 495
AfaI GTAC 2 cut(s) 200, 567
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 4 cut(s) 294, 308, 374, 555
AjnI CCWGG 2 cut(s) 115, 242
AluBI AGCT 6 cut(s) 48, 185, 251, 431, 499, 537
AluI AGCT 6 cut(s) 48, 185, 251, 431, 499, 537
AlwI GGATC 4 cut(s) 146, 427, 641, 659
AoxI GGCC 3 cut(s) 245, 514, 664
ApeKI GCWGC 3 cut(s) 48, 428, 431
AspS9I GGNCC 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 186, 469
AvaII GGWCC 1 cut(s) 482
BaeGI GKGCMC 1 cut(s) 20
BalI TGGCCA 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 588
BbvI GCAGC 3 cut(s) 35, 418, 440
BccI CCATC 3 cut(s) 391, 473, 642
BciT130I CCWGG 2 cut(s) 117, 244
BclI TGATCA 1 cut(s) 205
BfaI CTAG 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 522
BisI GCNGC 4 cut(s) 49, 329, 429, 432
BlsI GCNGC 4 cut(s) 50, 330, 430, 433
Bme1390I CCNGG 2 cut(s) 117, 244
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 2 cut(s) 60, 240
BmrFI CCNGG 2 cut(s) 117, 244
BmsI GCATC 3 cut(s) 148, 198, 340
BpiI GAAGAC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 379, 664
BsaBI GATNNNNATC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 675
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 2 cut(s) 117, 244
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 454
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 3 cut(s) 377, 620, 675
BseNI ACTGG 1 cut(s) 675
BseRI GAGGAG 1 cut(s) 258
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 3 cut(s) 35, 418, 440
BshFI GGCC 3 cut(s) 247, 516, 666
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 2 cut(s) 33, 624
BsnI GGCC 3 cut(s) 247, 516, 666
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 6 cut(s) 140, 151, 205, 419, 633, 651
BspACI CCGC 1 cut(s) 328
BspANI GGCC 3 cut(s) 247, 516, 666
BspCNI CTCAG 3 cut(s) 378, 621, 674
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 60, 240
BspPI GGATC 4 cut(s) 146, 427, 641, 659
BsrI ACTGG 1 cut(s) 675
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 6 cut(s) 140, 151, 205, 419, 633, 651
Bst2UI CCWGG 2 cut(s) 117, 244
Bst4CI ACNGT 4 cut(s) 67, 98, 198, 594
BstC8I GCNNGC 2 cut(s) 187, 249
BstDEI CTNAG 3 cut(s) 386, 629, 661
BstF5I GGATG 1 cut(s) 454
BstKTI GATC 6 cut(s) 143, 154, 208, 422, 636, 654
BstMBI GATC 6 cut(s) 140, 151, 205, 419, 633, 651
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 2 cut(s) 117, 244
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 2 cut(s) 115, 242
BstSLI GKGCMC 1 cut(s) 20
BstV1I GCAGC 3 cut(s) 35, 418, 440
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 1 cut(s) 633
BstYI RGATCY 1 cut(s) 633
BsuRI GGCC 3 cut(s) 247, 516, 666
BtsCI GGATG 1 cut(s) 454
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 187, 249
CciI TCATGA 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 482
Csp6I GTAC 2 cut(s) 199, 566
CviAII CATG 4 cut(s) 32, 209, 415, 583
CviQI GTAC 2 cut(s) 199, 566
DdeI CTNAG 3 cut(s) 386, 629, 661
DpnI GATC 6 cut(s) 142, 153, 207, 421, 635, 653
DpnII GATC 6 cut(s) 140, 151, 205, 419, 633, 651
EaeI YGGCCR 1 cut(s) 245
Eco147I AGGCCT 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 482
Eco57I CTGAAG 2 cut(s) 458, 495
EcoRII CCWGG 2 cut(s) 115, 242
EcoT22I ATGCAT 1 cut(s) 33
FaeI CATG 4 cut(s) 35, 212, 418, 586
FaqI GGGAC 1 cut(s) 455
FatI CATG 4 cut(s) 31, 208, 414, 582
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 4 cut(s) 49, 329, 429, 432
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 4 cut(s) 49, 329, 429, 432
FspBI CTAG 1 cut(s) 252
GluI GCNGC 4 cut(s) 49, 329, 429, 432
HaeIII GGCC 3 cut(s) 247, 516, 666
Hin1II CATG 4 cut(s) 35, 212, 418, 586
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 3 cut(s) 352, 361, 374
HphI GGTGA 2 cut(s) 186, 469
Hpy188I TCNGA 6 cut(s) 145, 205, 387, 438, 603, 630
Hpy188III TCNNGA 3 cut(s) 155, 209, 358
HpyAV CCTTC 2 cut(s) 400, 433
HpyCH4III ACNGT 4 cut(s) 67, 98, 198, 594
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 7 cut(s) 31, 51, 106, 161, 189, 428, 624
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 3 cut(s) 386, 629, 661
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 4 cut(s) 35, 212, 418, 586
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 6 cut(s) 140, 151, 205, 419, 633, 651
LmnI GCTCC 2 cut(s) 244, 271
Lsp1109I GCAGC 3 cut(s) 35, 418, 440
LweI GCATC 3 cut(s) 148, 198, 340
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 1 cut(s) 638
MalI GATC 6 cut(s) 142, 153, 207, 421, 635, 653
MboI GATC 6 cut(s) 140, 151, 205, 419, 633, 651
MboII GAAGA 2 cut(s) 488, 588
MflI RGATCY 1 cut(s) 633
MhlI GDGCHC 1 cut(s) 20
MlsI TGGCCA 1 cut(s) 247
MluCI AATT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 247
MlyI GAGTC 1 cut(s) 370
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 6 cut(s) 141, 279, 381, 506, 527, 624
Mox20I TGGCCA 1 cut(s) 247
Mph1103I ATGCAT 1 cut(s) 33
MscI TGGCCA 1 cut(s) 247
Msp20I TGGCCA 1 cut(s) 247
MspA1I CMGCKG 2 cut(s) 431, 537
MspR9I CCNGG 2 cut(s) 117, 244
Mva1269I GAATGC 2 cut(s) 33, 624
MvaI CCWGG 2 cut(s) 117, 244
MwoI GCNNNNNNNGC 1 cut(s) 522
NdeII GATC 6 cut(s) 140, 151, 205, 419, 633, 651
NlaIII CATG 4 cut(s) 35, 212, 418, 586
NlaIV GGNNCC 2 cut(s) 60, 240
NsiI ATGCAT 1 cut(s) 33
NspI RCATGY 1 cut(s) 35
PagI TCATGA 1 cut(s) 208
PceI AGGCCT 1 cut(s) 516
PctI GAATGC 2 cut(s) 33, 624
PfeI GAWTC 2 cut(s) 352, 374
PkrI GCNGC 4 cut(s) 50, 330, 430, 433
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
PsiI TTATAA 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 115, 242
PspGI CCWGG 2 cut(s) 115, 242
PspN4I GGNNCC 2 cut(s) 60, 240
PspPI GGNCC 1 cut(s) 482
PsuI RGATCY 1 cut(s) 633
PvuII CAGCTG 2 cut(s) 431, 537
RsaI GTAC 2 cut(s) 200, 567
RsaNI GTAC 2 cut(s) 199, 566
SatI GCNGC 4 cut(s) 49, 329, 429, 432
Sau3AI GATC 6 cut(s) 140, 151, 205, 419, 633, 651
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 2 cut(s) 117, 244
SduI GDGCHC 1 cut(s) 20
SetI ASST 8 cut(s) 50, 118, 130, 187, 253, 433, 501, 539
SfaNI GCATC 3 cut(s) 148, 198, 340
SinI GGWCC 1 cut(s) 482
SmlI CTYRAG 2 cut(s) 358, 643
SmoI CTYRAG 2 cut(s) 358, 643
Sse9I AATT 1 cut(s) 343
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 1 cut(s) 328
SspMI CTAG 1 cut(s) 252
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 2 cut(s) 115, 242
TaaI ACNGT 4 cut(s) 67, 98, 198, 594
TaiI ACGT 1 cut(s) 130
TaqI TCGA 2 cut(s) 271, 334
TasI AATT 1 cut(s) 343
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 2 cut(s) 352, 374
TscAI CASTG 1 cut(s) 626
TseI GCWGC 3 cut(s) 48, 428, 431
TspDTI ATGAA 3 cut(s) 312, 419, 476
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 482
XceI RCATGY 1 cut(s) 35
XcmI CCANNNNNNNNNTGG 2 cut(s) 69, 162
XspI CTAG 1 cut(s) 252
Zsp2I ATGCAT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.