MD10G1294000.v1.1

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000650
Physical Location & Seq
Reverse (-)
38212951 .. 38214688
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1294000.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGTCCGTACAACAGGCATTAGAAGAAAACAAACTGTTCGTACTAGACTATCATGACATTTTCCTTCCATTTCTGGACCAAATAAACGCTTTCAATGATCGAAAAGCTTATGGAACGCGTACAATTTTGTTCTTGACGTCAATGGGAACGTTAAAGCCTATTGCTATAGAACTCAGCTCTTCTGCAACCAACTCAGTTTCACCATCTAAGCAGGTTCTCACTCCTCCAGTTGATGCTACAACAAATTGGCTTTGGCAGCTCGGTAAAGTCCATGTATGCTCCAACGACGCCTGCGTTCATCAACTGGTTCACCACTGGTTACGCACTCACGCCTGCATGGAGCCATTCATAATAGCAGCCCACCGGCAGCTGAGTGTGATGCATCCAATTTACAAGCTTCTCAGTCCTCACATGCGGTACACCTTGAAGATAAATGCAATGGCCCGTCAGTTTCTCATCAACGCTGGAGGGATAATCGAGTCTGATTTCAGTGCCGGTAGATACAGCATGGAGATTAGTTGTGCTGCTTATCGAGATTGGTGGCGCTTTGACCTTCAGGGCCTTCCTGCAGATCTGATCAGAAGAGGAATGGCAATACCGGACTCTACCCAAGTCCACGGGATACGTCTACTGGTCGAAGATTACCCTTATGCAACGGATGGACTCCTCATATGGTCGGCAATGGAGACATTGGTTCGGACCTATGTCGATTACTACTATCCTGATGCAAGTGTGGTTCATTGTGATACAGAACTCCAGGCATGGTACAACGAGTCAATCAATGTAGGCCATGGCGATCTTCACCATGCTAGCTGGTGGCCGAAACTCTCAACTCCAGATGATCTTATCTCCATACTCACCACCATCATTTGGGTCTCATCAGCACAGCACGCAGCACTGAACTACGGGCAATATCCATATGGTGGGTATGTCCCGAACCGCCCACCACACATGCGGAGACTATTGCCGCAGGAGCATGATCCCGAGTATGTTGCTTTCATAAGAGATCCACAGAAATACTTTCTATCATCCCTGCCAGGTTTATTTGAAGCAACGAAGTACATGGCTGTAATAGATATCCTTTCAACGCACTCACCGGATGAGGTATATATCGGGGGGAGAAAAGACTTGTCAACCTGGTCAGCTGACACGGTGATCACCGAGGCATTCTACAGATTTTCAATGGAGATGAAGTGCATTGAGAAAGAGATTGAACAAAGAAATGCCGACTTAAACCTTCGGAACAGATGTGGGGCTGGAGTGTCACCATACCAGCTGCTCATGCCAAGCTCAGAACCAGGGATTACGGGCAGAGGAGTGCCGAATAGTATATCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00591 ko00592 ko01100 ko01110 map00591 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipoxygenase PLAT

Protein Analysis

447

Amino Acids

50.66

Weight (kDa)

5.96

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipoxygenase PF00305 1 - 429 1.6e-210 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 202
AccB7I CCANNNNNTGG 2 cut(s) 870, 923
AccI GTMKAC 1 cut(s) 628
AccII CGCG 1 cut(s) 118
AciI CCGC 4 cut(s) 415, 940, 955, 968
AclI AACGTT 1 cut(s) 149
AclWI GGATC 2 cut(s) 974, 1001
AcoI YGGCCR 1 cut(s) 818
AcuI CTGAAG 1 cut(s) 539
AcyI GRCGYC 2 cut(s) 137, 288
AfaI GTAC 6 cut(s) 9, 42, 121, 419, 767, 1061
AfiI CCNNNNNNNGG 2 cut(s) 870, 923
AflIII ACRYGT 1 cut(s) 116
AgsI TTSAA 6 cut(s) 94, 427, 1049, 1086, 1182, 1214
AjnI CCWGG 4 cut(s) 756, 1036, 1136, 1297
AluBI AGCT 9 cut(s) 107, 177, 259, 370, 397, 813, 1145, 1276, 1290
AluI AGCT 9 cut(s) 107, 177, 259, 370, 397, 813, 1145, 1276, 1290
Alw26I GTCTC 3 cut(s) 680, 880, 952
AlwI GGATC 2 cut(s) 974, 1001
Ama87I CYCGRG 1 cut(s) 983
AoxI GGCC 4 cut(s) 441, 559, 787, 818
ApeKI GCWGC 6 cut(s) 256, 356, 367, 524, 893, 1276
AspLEI GCGC 1 cut(s) 546
AspS9I GGNCC 4 cut(s) 76, 442, 559, 699
AsuHPI GGTGA 8 cut(s) 192, 302, 794, 850, 1086, 1150, 1165, 1257
AsuNHI GCTAGC 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 983
AvaII GGWCC 2 cut(s) 76, 699
BbvI GCAGC 6 cut(s) 268, 368, 379, 511, 905, 1263
BccI CCATC 3 cut(s) 211, 653, 872
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 4 cut(s) 758, 1038, 1138, 1299
BciVI GTATCC 1 cut(s) 615
BclI TGATCA 2 cut(s) 576, 1155
BcoDI GTCTC 3 cut(s) 680, 880, 952
BfaI CTAG 2 cut(s) 44, 810
BfmI CTRYAG 3 cut(s) 165, 567, 1171
BfoI RGCGCY 1 cut(s) 547
BfuAI ACCTGC 1 cut(s) 202
BfuI GTATCC 1 cut(s) 615
BglII AGATCT 1 cut(s) 571
BisI GCNGC 7 cut(s) 257, 357, 368, 525, 894, 968, 1277
BlsI GCNGC 7 cut(s) 258, 358, 369, 526, 895, 969, 1278
Bme1390I CCNGG 4 cut(s) 758, 1038, 1138, 1299
Bme18I GGWCC 2 cut(s) 76, 699
BmeT110I CYCGRG 1 cut(s) 983
BmgT120I GGNCC 4 cut(s) 76, 442, 559, 699
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 4 cut(s) 758, 1038, 1138, 1299
BmsI GCATC 4 cut(s) 223, 369, 391, 715
BmtI GCTAGC 1 cut(s) 813
BoxI GACNNNNGTC 1 cut(s) 704
BpmI CTGGAG 5 cut(s) 210, 486, 740, 819, 1278
BsaHI GRCGYC 2 cut(s) 137, 288
BsaI GGTCTC 1 cut(s) 880
BsaJI CCNNGG 4 cut(s) 616, 790, 1161, 1298
BsaWI WCCGGW 2 cut(s) 598, 1096
BsaXI ACNNNNNCTCC 2 cut(s) 1178, 1208
Bsc4I CCNNNNNNNGG 2 cut(s) 870, 923
Bse118I RCCGGY 2 cut(s) 363, 494
Bse1I ACTGG 4 cut(s) 227, 309, 320, 636
Bse3DI GCAATG 2 cut(s) 444, 687
BseBI CCWGG 4 cut(s) 758, 1038, 1138, 1299
BseDI CCNNGG 4 cut(s) 616, 790, 1161, 1298
BseGI GGATG 4 cut(s) 382, 664, 1028, 1105
BseLI CCNNNNNNNGG 2 cut(s) 870, 923
BseMI GCAATG 2 cut(s) 444, 687
BseMII CTCAG 5 cut(s) 187, 207, 362, 415, 1305
BseNI ACTGG 4 cut(s) 227, 309, 320, 636
BseRI GAGGAG 3 cut(s) 213, 656, 1329
BseXI GCAGC 6 cut(s) 268, 368, 379, 511, 905, 1263
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 4 cut(s) 443, 561, 789, 820
BsiHKCI CYCGRG 1 cut(s) 983
BsiSI CCGG 4 cut(s) 364, 495, 599, 1097
BslFI GGGAC 1 cut(s) 917
BslI CCNNNNNNNGG 2 cut(s) 870, 923
BsmAI GTCTC 3 cut(s) 680, 880, 952
BsmFI GGGAC 1 cut(s) 917
BsmI GAATGC 1 cut(s) 1166
BsnI GGCC 4 cut(s) 443, 561, 789, 820
Bso31I GGTCTC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 983
Bsp143I GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
Bsp19I CCATGG 1 cut(s) 790
BspACI CCGC 4 cut(s) 415, 940, 955, 968
BspANI GGCC 4 cut(s) 443, 561, 789, 820
BspCNI CTCAG 5 cut(s) 186, 206, 363, 414, 1304
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 342
BspMAI CTGCAG 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 202
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 2 cut(s) 974, 1001
BspQI GCTCTTC 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 880
BsrDI GCAATG 2 cut(s) 444, 687
BsrFI RCCGGY 2 cut(s) 363, 494
BsrI ACTGG 4 cut(s) 227, 309, 320, 636
BssAI RCCGGY 2 cut(s) 363, 494
BssECI CCNNGG 4 cut(s) 616, 790, 1161, 1298
BssMI GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
BssNI GRCGYC 2 cut(s) 137, 288
BssT1I CCWWGG 1 cut(s) 790
Bst2UI CCWGG 4 cut(s) 758, 1038, 1138, 1299
Bst4CI ACNGT 2 cut(s) 36, 1153
Bst6I CTCTTC 2 cut(s) 184, 577
BstACI GRCGYC 2 cut(s) 137, 288
BstC8I GCNNGC 4 cut(s) 292, 334, 811, 891
BstDEI CTNAG 6 cut(s) 173, 193, 207, 371, 401, 1291
BstDSI CCRYGG 2 cut(s) 616, 790
BstF5I GGATG 4 cut(s) 382, 664, 1028, 1105
BstFNI CGCG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 8 cut(s) 100, 574, 579, 799, 844, 982, 1009, 1158
BstMAI GTCTC 3 cut(s) 680, 880, 952
BstMBI GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
BstMWI GCNNNNNNNGC 4 cut(s) 256, 890, 973, 1282
BstNI CCWGG 4 cut(s) 758, 1038, 1138, 1299
BstNSI RCATGY 2 cut(s) 415, 955
BstPAI GACNNNNGTC 1 cut(s) 704
BstSCI CCNGG 4 cut(s) 756, 1036, 1136, 1297
BstSFI CTRYAG 3 cut(s) 165, 567, 1171
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 6 cut(s) 268, 368, 379, 511, 905, 1263
BstX2I RGATCY 2 cut(s) 571, 1006
BstYI RGATCY 2 cut(s) 571, 1006
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 4 cut(s) 443, 561, 789, 820
BtgI CCRYGG 2 cut(s) 616, 790
BtsCI GGATG 4 cut(s) 382, 664, 1028, 1105
BtsIMutI CAGTG 3 cut(s) 313, 496, 896
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 4 cut(s) 292, 334, 811, 891
CciI TCATGA 1 cut(s) 52
CfoI GCGC 1 cut(s) 546
Cfr10I RCCGGY 2 cut(s) 363, 494
Cfr13I GGNCC 4 cut(s) 76, 442, 559, 699
CseI GACGC 1 cut(s) 296
CsiI ACCWGGT 1 cut(s) 1136
Csp6I GTAC 6 cut(s) 8, 41, 120, 418, 766, 1060
CviQI GTAC 6 cut(s) 8, 41, 120, 418, 766, 1060
DdeI CTNAG 6 cut(s) 173, 193, 207, 371, 401, 1291
DpnI GATC 8 cut(s) 99, 573, 578, 798, 843, 981, 1008, 1157
DpnII GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 2 cut(s) 184, 577
EarI CTCTTC 2 cut(s) 184, 577
Eco130I CCWWGG 1 cut(s) 790
Eco31I GGTCTC 1 cut(s) 880
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 2 cut(s) 76, 699
Eco57I CTGAAG 1 cut(s) 539
Eco88I CYCGRG 1 cut(s) 983
EcoO109I RGGNCCY 1 cut(s) 559
EcoRII CCWGG 4 cut(s) 756, 1036, 1136, 1297
EcoRV GATATC 1 cut(s) 1078
EcoT14I CCWWGG 1 cut(s) 790
EcoT22I ATGCAT 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 790
FaqI GGGAC 1 cut(s) 917
FauNDI CATATG 2 cut(s) 671, 919
FbaI TGATCA 2 cut(s) 576, 1155
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 7 cut(s) 257, 357, 368, 525, 894, 968, 1277
FokI GGATG 4 cut(s) 369, 671, 1015, 1112
Fsp4HI GCNGC 7 cut(s) 257, 357, 368, 525, 894, 968, 1277
FspBI CTAG 2 cut(s) 44, 810
GlaI GCGC 1 cut(s) 545
GluI GCNGC 7 cut(s) 257, 357, 368, 525, 894, 968, 1277
GsuI CTGGAG 5 cut(s) 210, 486, 740, 819, 1278
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 4 cut(s) 443, 561, 789, 820
HapII CCGG 4 cut(s) 364, 495, 599, 1097
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 546
Hin1I GRCGYC 2 cut(s) 137, 288
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HincII GTYRAC 1 cut(s) 1134
HindII GTYRAC 1 cut(s) 1134
HindIII AAGCTT 2 cut(s) 105, 395
HinfI GANTC 4 cut(s) 479, 602, 663, 773
HpaII CCGG 4 cut(s) 364, 495, 599, 1097
HphI GGTGA 8 cut(s) 192, 302, 794, 850, 1086, 1150, 1165, 1257
Hpy166II GTNNAC 5 cut(s) 310, 420, 616, 629, 1134
Hpy188I TCNGA 7 cut(s) 484, 576, 581, 699, 1242, 1294, 1340
Hpy188III TCNNGA 8 cut(s) 53, 74, 133, 533, 722, 836, 934, 983
Hpy8I GTNNAC 5 cut(s) 310, 420, 616, 629, 1134
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 4 cut(s) 74, 563, 572, 1247
HpyCH4III ACNGT 2 cut(s) 36, 1153
HpyCH4IV ACGT 3 cut(s) 137, 149, 625
HpyCH4V TGCA 8 cut(s) 185, 336, 382, 437, 569, 653, 728, 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 890, 973, 1282
HpyF3I CTNAG 6 cut(s) 173, 193, 207, 371, 401, 1291
HpySE526I ACGT 3 cut(s) 137, 149, 625
Hsp92I GRCGYC 2 cut(s) 137, 288
HspAI GCGC 1 cut(s) 544
Ksp22I TGATCA 2 cut(s) 576, 1155
Kzo9I GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 3 cut(s) 284, 340, 973
Lsp1109I GCAGC 6 cut(s) 268, 368, 379, 511, 905, 1263
LweI GCATC 4 cut(s) 223, 369, 391, 715
MabI ACCWGGT 1 cut(s) 1136
MaeI CTAG 2 cut(s) 44, 810
MaeII ACGT 3 cut(s) 137, 149, 625
MaeIII GTNAC 2 cut(s) 318, 1263
MalI GATC 8 cut(s) 99, 573, 578, 798, 843, 981, 1008, 1157
MboI GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
MboII GAAGA 6 cut(s) 35, 171, 439, 594, 650, 791
MflI RGATCY 2 cut(s) 571, 1006
MluCI AATT 3 cut(s) 123, 244, 387
MluI ACGCGT 1 cut(s) 116
MlyI GAGTC 4 cut(s) 488, 596, 657, 782
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 8 cut(s) 234, 417, 461, 578, 677, 1096, 1156, 1307
Mph1103I ATGCAT 1 cut(s) 384
MseI TTAA 2 cut(s) 152, 1232
MspA1I CMGCKG 3 cut(s) 370, 1145, 1276
MspI CCGG 4 cut(s) 364, 495, 599, 1097
MspR9I CCNGG 4 cut(s) 758, 1038, 1138, 1299
Mva1269I GAATGC 1 cut(s) 1166
MvaI CCWGG 4 cut(s) 758, 1038, 1138, 1299
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 4 cut(s) 256, 890, 973, 1282
NcoI CCATGG 1 cut(s) 790
NdeI CATATG 2 cut(s) 671, 919
NdeII GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
NheI GCTAGC 1 cut(s) 809
NlaIV GGNNCC 1 cut(s) 342
NmuCI GTSAC 1 cut(s) 1263
NsiI ATGCAT 1 cut(s) 384
NspI RCATGY 2 cut(s) 415, 955
PagI TCATGA 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 184
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 1166
PflMI CCANNNNNTGG 2 cut(s) 870, 923
PkrI GCNGC 7 cut(s) 258, 358, 369, 526, 895, 969, 1278
PleI GAGTC 4 cut(s) 487, 596, 657, 781
PpsI GAGTC 4 cut(s) 487, 596, 657, 781
PshAI GACNNNNGTC 1 cut(s) 704
Psp1406I AACGTT 1 cut(s) 149
Psp6I CCWGG 4 cut(s) 756, 1036, 1136, 1297
PspGI CCWGG 4 cut(s) 756, 1036, 1136, 1297
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 4 cut(s) 76, 442, 559, 699
PstI CTGCAG 1 cut(s) 571
PsuI RGATCY 2 cut(s) 571, 1006
PvuII CAGCTG 3 cut(s) 370, 1145, 1276
RsaI GTAC 6 cut(s) 9, 42, 121, 419, 767, 1061
RsaNI GTAC 6 cut(s) 8, 41, 120, 418, 766, 1060
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 152, 1232
SatI GCNGC 7 cut(s) 257, 357, 368, 525, 894, 968, 1277
Sau3AI GATC 8 cut(s) 97, 571, 576, 796, 841, 979, 1006, 1155
Sau96I GGNCC 4 cut(s) 76, 442, 559, 699
SchI GAGTC 4 cut(s) 488, 596, 657, 782
ScrFI CCNGG 4 cut(s) 758, 1038, 1138, 1299
SexAI ACCWGGT 1 cut(s) 1136
SfaNI GCATC 4 cut(s) 223, 369, 391, 715
SfcI CTRYAG 3 cut(s) 165, 567, 1171
SinI GGWCC 2 cut(s) 76, 699
Sse9I AATT 3 cut(s) 123, 244, 387
SsiI CCGC 4 cut(s) 415, 940, 955, 968
SspMI CTAG 2 cut(s) 44, 810
StyD4I CCNGG 4 cut(s) 756, 1036, 1136, 1297
StyI CCWWGG 1 cut(s) 790
TaaI ACNGT 2 cut(s) 36, 1153
TaiI ACGT 3 cut(s) 140, 152, 628
TaqI TCGA 5 cut(s) 100, 477, 532, 636, 708
TasI AATT 3 cut(s) 123, 244, 387
TatI WGTACW 1 cut(s) 1059
TauI GCSGC 1 cut(s) 970
Tru1I TTAA 2 cut(s) 152, 1232
Tru9I TTAA 2 cut(s) 152, 1232
TscAI CASTG 3 cut(s) 320, 496, 903
TseFI GTSAC 1 cut(s) 1263
TseI GCWGC 6 cut(s) 256, 356, 367, 524, 893, 1276
Tsp45I GTSAC 1 cut(s) 1263
TspDTI ATGAA 5 cut(s) 287, 337, 728, 988, 1205
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 3 cut(s) 320, 496, 903
Van91I CCANNNNNTGG 2 cut(s) 870, 923
VpaK11BI GGWCC 2 cut(s) 76, 699
XceI RCATGY 2 cut(s) 415, 955
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 2 cut(s) 44, 810
ZraI GACGTC 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.