Rh5BG062300

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000650
Physical Location & Seq
Forward (+)
5121820 .. 5123775
1956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG062300.1

Sequence Viewer

Length: 1239 bp
ATGGGTGCTGGGAAGTGCATTACTTACCAAGTTAAATCTGTGGTGGATTCAAACTTTGGGACGCCAGGAGCCATCACAGTCAGCAACAGATACAACAGCGAGTTTTATTTAGATAGTATCAACATTGAAGGTGTTGTTCATTTTGCCTGCAACTCCTGGGTTCAATCAGGGCATGCTAACCCTGCAAAAAGAATCTTCTTTCCTACCAAGGCATACTTACCTAGTGAGACACCAGCAGGGATTAAAGAGCTAAGAGAAATGGAGCTAATTCAACTAAGGGGTGATGGGACAGGACTTCGAAAGCCATCTGATAGAATATACGACTACAATACTCACAGCGACATTGGAAATCCAGACAAGGGAATTGAATACATTAGACCCATTCTTGGAGGCAGCAATCACCCATACCCTAGACGCTGTCGAACTGGAGGTCCTCCAACCAAGACAGATGAGAAACTGGAGTCACCAGTAAATGAATTCCTGACACTTTATGTGCCTAGAGATGAAGAGTTTGAGGAGTCCAAGAGAGAAGCTGTTGATCTTGGAAGGTTAAAGGCAATTATGAGGCACATTATTCCTGCACTAACAAAAGTTGCCAGGGATAGTGATGAATTCAAGGCATATTCAGACATAACTGGTCTGTATAGAGACGGCATGCCTTCAATAACAAAGAATATGAGGAATCTTCCCTTGCCAAATATTTTGAACAAAGTCCAGGAATACCTAAAATTTGACCCAACAAAATTAAACTCGAGGTTTACAGCTTGTGGTTTGCCAGACGACGAATTTGGACGTCAAGCAATTGCAGGCATAAATCCTCTAAGCATAGAGAGGCTGACAACTTTCCCACCTGCATTGGAAGAAAACAAGCTGTTCATACTAGACTATCATGACATTTTCCTTCCGTTTATCAATCAAATAAATGCTATGGATGACCGAAAAGCTTATGCAACACGTACAATTTTCTTCCTCACCTCATTGGGAACATTGAAGCCCATTGCTATTGAGCTCAGTCTCCATCAAACCAATTCAGATACACCATCCAAGCAGGTTCTCACTCCTCCAGTTGATGCTACAACAAATTGGCTTTTGGGAACTTGGCAAAGCCCATCAACCCACCGGCAGCTGAGTGCGATGCATCCCATCTACAGGCTTCTCAAACCTCACATGCGATACACAATGAAGATAAATGCATTGGCCCGTCAGTTTCTGATCAATTCTGAAGGAATAATCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00591 ko00592 ko01100 ko01110 map00591 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipoxygenase PLAT

Protein Analysis

412

Amino Acids

46.56

Weight (kDa)

8.19

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAT PF01477 8 - 67 6.3e-06 PLAT/LH2 domain
Lipoxygenase PF00305 80 - 284 2.2e-56 Lipoxygenase
Lipoxygenase PF00305 285 - 363 2.1e-27 Lipoxygenase
Lipoxygenase PF00305 371 - 412 1.5e-18 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 859
AatII GACGTC 1 cut(s) 796
Acc36I ACCTGC 2 cut(s) 859, 1039
AcsI RAATTY 4 cut(s) 476, 611, 728, 785
AcyI GRCGYC 2 cut(s) 62, 793
AfaI GTAC 1 cut(s) 958
AfiI CCNNNNNNNGG 3 cut(s) 359, 386, 1149
AflIII ACRYGT 1 cut(s) 953
AgsI TTSAA 9 cut(s) 51, 128, 164, 272, 368, 616, 663, 706, 991
AjnI CCWGG 4 cut(s) 64, 155, 596, 714
AloI GAACNNNNNNTCC 2 cut(s) 415, 447
AluBI AGCT 8 cut(s) 250, 265, 533, 764, 871, 944, 1009, 1126
AluI AGCT 8 cut(s) 250, 265, 533, 764, 871, 944, 1009, 1126
Alw21I GWGCWC 1 cut(s) 1011
Alw26I GTCTC 3 cut(s) 221, 642, 1019
AlwNI CAGNNNCTG 1 cut(s) 1210
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 1 cut(s) 1197
ApeKI GCWGC 2 cut(s) 393, 1123
ApoI RAATTY 4 cut(s) 476, 611, 728, 785
ArsI GACNNNNNNTTYG 4 cut(s) 415, 447, 770, 802
AspS9I GGNCC 2 cut(s) 431, 1198
AsuHPI GGTGA 4 cut(s) 293, 392, 456, 964
AsuII TTCGAA 1 cut(s) 298
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 1011
BbvI GCAGC 2 cut(s) 405, 1135
BccI CCATC 7 cut(s) 80, 278, 313, 1026, 1048, 1117, 1151
BceAI ACGGC 1 cut(s) 667
BciT130I CCWGG 4 cut(s) 66, 157, 598, 716
BclI TGATCA 1 cut(s) 1212
BcoDI GTCTC 3 cut(s) 221, 642, 1019
BfaI CTAG 4 cut(s) 222, 411, 498, 881
BfmI CTRYAG 1 cut(s) 1147
BfuAI ACCTGC 2 cut(s) 859, 1039
BisI GCNGC 2 cut(s) 394, 1124
BlsI GCNGC 2 cut(s) 395, 1125
Bme1390I CCNGG 4 cut(s) 66, 157, 598, 716
Bme18I GGWCC 1 cut(s) 431
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 2 cut(s) 431, 1198
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 4 cut(s) 66, 157, 598, 716
BmsI GCATC 3 cut(s) 1060, 1125, 1147
BpmI CTGGAG 3 cut(s) 447, 479, 1047
Bpu14I TTCGAA 1 cut(s) 298
BsaAI YACGTR 1 cut(s) 956
BsaHI GRCGYC 2 cut(s) 62, 793
BsaJI CCNNGG 3 cut(s) 156, 207, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 386, 1149
Bse118I RCCGGY 1 cut(s) 1119
Bse1I ACTGG 5 cut(s) 430, 462, 467, 640, 1064
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 4 cut(s) 66, 157, 598, 716
BseDI CCNNGG 3 cut(s) 156, 207, 597
BseGI GGATG 3 cut(s) 937, 1040, 1138
BseLI CCNNNNNNNGG 3 cut(s) 359, 386, 1149
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 2 cut(s) 1024, 1118
BseNI ACTGG 5 cut(s) 430, 462, 467, 640, 1064
BseRI GAGGAG 2 cut(s) 530, 1050
BseXI GCAGC 2 cut(s) 405, 1135
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 564
BshFI GGCC 1 cut(s) 1199
BsiHKAI GWGCWC 1 cut(s) 1011
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 1 cut(s) 1120
BslFI GGGAC 2 cut(s) 73, 301
BslI CCNNNNNNNGG 3 cut(s) 359, 386, 1149
BsmAI GTCTC 3 cut(s) 221, 642, 1019
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 2 cut(s) 73, 301
BsnI GGCC 1 cut(s) 1199
BsoBI CYCGRG 1 cut(s) 751
Bsp119I TTCGAA 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 1011
Bsp143I GATC 2 cut(s) 538, 1212
BspANI GGCC 1 cut(s) 1199
BspCNI CTCAG 2 cut(s) 1023, 1119
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 1 cut(s) 70
BspMI ACCTGC 2 cut(s) 859, 1039
BspT104I TTCGAA 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 1119
BsrI ACTGG 5 cut(s) 430, 462, 467, 640, 1064
BssAI RCCGGY 1 cut(s) 1119
BssECI CCNNGG 3 cut(s) 156, 207, 597
BssMI GATC 2 cut(s) 538, 1212
BssNI GRCGYC 2 cut(s) 62, 793
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 4 cut(s) 66, 157, 598, 716
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 501
BstACI GRCGYC 2 cut(s) 62, 793
BstBAI YACGTR 1 cut(s) 956
BstBI TTCGAA 1 cut(s) 298
BstC8I GCNNGC 4 cut(s) 148, 174, 656, 808
BstDEI CTNAG 5 cut(s) 251, 275, 821, 1010, 1127
BstF5I GGATG 3 cut(s) 937, 1040, 1138
BstKTI GATC 2 cut(s) 541, 1215
BstMAI GTCTC 3 cut(s) 221, 642, 1019
BstMBI GATC 2 cut(s) 538, 1212
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 4 cut(s) 66, 157, 598, 716
BstNSI RCATGY 3 cut(s) 176, 658, 1171
BstSCI CCNGG 4 cut(s) 64, 155, 596, 714
BstSFI CTRYAG 1 cut(s) 1147
BstV1I GCAGC 2 cut(s) 405, 1135
BsuRI GGCC 1 cut(s) 1199
BtgZI GCGATG 1 cut(s) 1148
BtsCI GGATG 3 cut(s) 937, 1040, 1138
BveI ACCTGC 2 cut(s) 859, 1039
Cac8I GCNNGC 4 cut(s) 148, 174, 656, 808
CaiI CAGNNNCTG 1 cut(s) 1210
CciI TCATGA 1 cut(s) 889
Cfr10I RCCGGY 1 cut(s) 1119
Cfr13I GGNCC 2 cut(s) 431, 1198
CseI GACGC 2 cut(s) 70, 423
Csp6I GTAC 1 cut(s) 957
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviAII CATG 4 cut(s) 173, 655, 890, 1168
CviQI GTAC 1 cut(s) 957
DdeI CTNAG 5 cut(s) 251, 275, 821, 1010, 1127
DpnI GATC 2 cut(s) 540, 1214
DpnII GATC 2 cut(s) 538, 1212
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Ecl136II GAGCTC 1 cut(s) 1009
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 1011
Eco47I GGWCC 1 cut(s) 431
Eco53kI GAGCTC 1 cut(s) 1009
Eco88I CYCGRG 1 cut(s) 751
EcoICRI GAGCTC 1 cut(s) 1009
EcoO109I RGGNCCY 1 cut(s) 431
EcoRI GAATTC 2 cut(s) 476, 611
EcoRII CCWGG 4 cut(s) 64, 155, 596, 714
EcoT14I CCWWGG 1 cut(s) 207
EcoT22I ATGCAT 2 cut(s) 1140, 1195
EcoT38I GRGCYC 1 cut(s) 1011
ErhI CCWWGG 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 4 cut(s) 176, 658, 893, 1171
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 2 cut(s) 73, 301
FatI CATG 4 cut(s) 172, 654, 889, 1167
FbaI TGATCA 1 cut(s) 1212
Fnu4HI GCNGC 2 cut(s) 394, 1124
FokI GGATG 3 cut(s) 944, 1027, 1125
FriOI GRGCYC 1 cut(s) 1011
Fsp4HI GCNGC 2 cut(s) 394, 1124
FspBI CTAG 4 cut(s) 222, 411, 498, 881
GluI GCNGC 2 cut(s) 394, 1124
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 3 cut(s) 447, 479, 1047
HaeIII GGCC 1 cut(s) 1199
HapII CCGG 1 cut(s) 1120
HgaI GACGC 2 cut(s) 70, 423
Hin1I GRCGYC 2 cut(s) 62, 793
Hin1II CATG 4 cut(s) 176, 658, 893, 1171
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 5 cut(s) 47, 192, 461, 518, 682
HpaII CCGG 1 cut(s) 1120
HphI GGTGA 4 cut(s) 293, 392, 456, 964
Hpy166II GTNNAC 1 cut(s) 759
Hpy188I TCNGA 5 cut(s) 310, 628, 1033, 1212, 1222
Hpy188III TCNNGA 3 cut(s) 353, 481, 890
Hpy8I GTNNAC 1 cut(s) 759
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 5 cut(s) 122, 540, 669, 911, 1217
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 2 cut(s) 793, 955
HpyCH4V TGCA 9 cut(s) 18, 150, 185, 581, 806, 854, 950, 1138, 1193
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 5 cut(s) 251, 275, 821, 1010, 1127
HpySE526I ACGT 2 cut(s) 793, 955
Hsp92I GRCGYC 2 cut(s) 62, 793
Hsp92II CATG 4 cut(s) 176, 658, 893, 1171
Ksp22I TGATCA 1 cut(s) 1212
Kzo9I GATC 2 cut(s) 538, 1212
LmnI GCTCC 2 cut(s) 68, 262
Lsp1109I GCAGC 2 cut(s) 405, 1135
LweI GCATC 3 cut(s) 1060, 1125, 1147
MaeI CTAG 4 cut(s) 222, 411, 498, 881
MaeII ACGT 2 cut(s) 793, 955
MaeIII GTNAC 1 cut(s) 462
MalI GATC 2 cut(s) 540, 1214
MboI GATC 2 cut(s) 538, 1212
MboII GAAGA 6 cut(s) 187, 518, 677, 872, 958, 1195
MfeI CAATTG 1 cut(s) 801
MhlI GDGCHC 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 470, 527
MmeI TCCRAC 1 cut(s) 461
Mph1103I ATGCAT 2 cut(s) 1140, 1195
MseI TTAA 4 cut(s) 33, 243, 551, 746
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 1 cut(s) 1120
MspR9I CCNGG 4 cut(s) 66, 157, 598, 716
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 4 cut(s) 66, 157, 598, 716
MwoI GCNNNNNNNGC 1 cut(s) 182
NdeII GATC 2 cut(s) 538, 1212
NlaIII CATG 4 cut(s) 176, 658, 893, 1171
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 462
NsiI ATGCAT 2 cut(s) 1140, 1195
NspI RCATGY 3 cut(s) 176, 658, 1171
NspV TTCGAA 1 cut(s) 298
PaeI GCATGC 2 cut(s) 176, 658
PaeR7I CTCGAG 1 cut(s) 751
PagI TCATGA 1 cut(s) 889
PaqCI CACCTGC 1 cut(s) 859
PfeI GAWTC 3 cut(s) 47, 192, 682
PflFI GACNNNGTC 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 714
PkrI GCNGC 2 cut(s) 395, 1125
PleI GAGTC 2 cut(s) 469, 526
PpsI GAGTC 2 cut(s) 469, 526
Ppu21I YACGTR 1 cut(s) 956
PpuMI RGGWCCY 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 1011
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 4 cut(s) 64, 155, 596, 714
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 4 cut(s) 64, 155, 596, 714
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 2 cut(s) 431, 1198
PspPPI RGGWCCY 1 cut(s) 431
PspXI VCTCGAGB 1 cut(s) 751
PstNI CAGNNNCTG 1 cut(s) 1210
PsyI GACNNNGTC 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 1126
RsaI GTAC 1 cut(s) 958
RsaNI GTAC 1 cut(s) 957
SacI GAGCTC 1 cut(s) 1011
SaqAI TTAA 4 cut(s) 33, 243, 551, 746
SatI GCNGC 2 cut(s) 394, 1124
Sau3AI GATC 2 cut(s) 538, 1212
Sau96I GGNCC 2 cut(s) 431, 1198
SchI GAGTC 2 cut(s) 470, 527
ScrFI CCNGG 4 cut(s) 66, 157, 598, 716
SduI GDGCHC 1 cut(s) 1011
SfaNI GCATC 3 cut(s) 1060, 1125, 1147
SfcI CTRYAG 1 cut(s) 1147
Sfr274I CTCGAG 1 cut(s) 751
SfuI TTCGAA 1 cut(s) 298
SinI GGWCC 1 cut(s) 431
SlaI CTCGAG 1 cut(s) 751
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
SphI GCATGC 2 cut(s) 176, 658
SspI AATATT 1 cut(s) 700
SspMI CTAG 4 cut(s) 222, 411, 498, 881
SstI GAGCTC 1 cut(s) 1011
StyD4I CCNGG 4 cut(s) 64, 155, 596, 714
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 2 cut(s) 796, 958
TaqI TCGA 4 cut(s) 298, 421, 752, 1233
TaqII GACCGA 1 cut(s) 951
TfiI GAWTC 3 cut(s) 47, 192, 682
Tru1I TTAA 4 cut(s) 33, 243, 551, 746
Tru9I TTAA 4 cut(s) 33, 243, 551, 746
TseFI GTSAC 1 cut(s) 462
TseI GCWGC 2 cut(s) 393, 1123
Tsp45I GTSAC 1 cut(s) 462
TspDTI ATGAA 6 cut(s) 128, 489, 519, 624, 865, 1196
TspGWI ACGGA 1 cut(s) 894
Tth111I GACNNNGTC 1 cut(s) 417
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 4 cut(s) 476, 611, 728, 785
XceI RCATGY 3 cut(s) 176, 658, 1171
XhoI CTCGAG 1 cut(s) 751
XspI CTAG 4 cut(s) 222, 411, 498, 881
ZraI GACGTC 1 cut(s) 794
Zsp2I ATGCAT 2 cut(s) 1140, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.