Rh5BG061900

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000650
Physical Location & Seq
Forward (+)
5089921 .. 5092499
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG061900.1

Sequence Viewer

Length: 1410 bp
ATGAAGCCAAACCTGAGTGAGGAAGCTATATTGGATTGGACAAGGAATTTGAAAATGGGTGCCGGGAAGACCACTACTTACCAAGTTAAATTTGTGGTGGATTCTAACTTTGGGATGCCAGGAGCCATCACGGTTAGCAACAGAGACCAGAGCGAGTTCTATTTGGATAGCATCAACTTTGAAGGTGTTATTAATTTTGCCTGCAACTCTTGGGTTCAACCAGAGTGCGAAAACCCTGCAAAAAGAATCTTCTTCTCTACCAATAAGGCATACTTACCTAGTGAAACACCAGCAGGGCTGAAAGAACTAAGAAAAATGGAGCTAATTCAACAGAGGGGTGATGGAACAGGATTTCGAAAACCATCTGATAGAATATACGATTATGACACTTACAATGACATTGGAAATCCTGATCTTGGATACATAAGGCCCATCCTTGGAGGCAACAAACACCCATACCCTAGACGCTGTCGAACTGGAAGGCCTCGAACCAAGACAGGGATTACATCTTCTTGTTTGCCAGATGACGAGTTTGGCCGTCAAGCACTTGCAGGCATAAATCCTCTAAGCATAGAGAGGCTCACAACTTTCCCACCTGTAAGCAAACTGAATGCCTCAACTTATGGCCCACAAGAATCGGAATTCAAGGAAGAACACATAATAGGCCATGTAAATGGCATGTCTATGCAACAGGCATTGGAAGAAAACAAACTGTTCATACTAGACTATCATGACATTTTCCTTCCGTTTATCAACCAAATAAATGCTATGGATGACCGAAAAGCTTATGCAACACGTACAATTTTTTTCCTCACCTCATTGGGAACATTAAAGCCCATTGCTATTGAGCTCAGTCTCCCTCAAACCAATTCAGACCCACCATCTAAGCACGTTCTCACTCCTCCAGTCGATGCTACAACAAGTTGGCTTTGGGAACTTGGCAAAGCCCATGTATGCTCCAATGATTCCTGCGTTCATCAACTCATTCATCACTGGTTGCGCAGCCATGCGTGTATGGAACCATTTATCATAGCAACTCACCGGCAGCTGAGTGCGATGCATCCCATCTACAGGCTTCTCAAACCTCACATGCGATACACGATGAAGATAAATGCATTGGCCCGTCAATTTCTGATCAATTCTGAAGGAATAATCGAGTCGACTTTCACTGCTGGCAAACATAGCATGGAGATTAGTTGTGCTGCATATCGAGACTGGTGGCGCTTTGACCTTGAGGGCCTTCCTGCAGATCTCATCAGAAGAGGGATGGCAATACCAGACCCCAGTCAGGTCCATGAACTAAGACTACTGATCGAAGATTATCCTTACGCAACAGATGGGCTTCTCATATGGACAGCAATTGAGAGATTGGTTCAAACCTATGTCCATTATTACTATGAAAGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00591 ko00592 ko01100 ko01110 map00591 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipoxygenase PLAT

Protein Analysis

469

Amino Acids

53.63

Weight (kDa)

6.6

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAT PF01477 7 - 85 1.5e-07 PLAT/LH2 domain
Lipoxygenase PF00305 99 - 168 1.1e-25 Lipoxygenase
Lipoxygenase PF00305 172 - 467 1.7e-151 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1001
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 1160
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 3 cut(s) 46, 89, 641
AcuI CTGAAG 1 cut(s) 1164
AfaI GTAC 1 cut(s) 799
AfiI CCNNNNNNNGG 6 cut(s) 19, 416, 437, 498, 1071, 1288
AflIII ACRYGT 1 cut(s) 794
AgsI TTSAA 6 cut(s) 52, 182, 218, 329, 646, 1376
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 5 cut(s) 26, 322, 785, 850, 1048
AluI AGCT 5 cut(s) 26, 322, 785, 850, 1048
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 3 cut(s) 138, 860, 1206
AoxI GGCC 7 cut(s) 428, 482, 535, 625, 664, 1119, 1237
ApeKI GCWGC 3 cut(s) 1002, 1045, 1202
ApoI RAATTY 3 cut(s) 46, 89, 641
ArsI GACNNNNNNTTYG 2 cut(s) 31, 63
AseI ATTAAT 1 cut(s) 192
AspLEI GCGC 2 cut(s) 1002, 1224
AspS9I GGNCC 5 cut(s) 429, 626, 1120, 1237, 1291
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 3 cut(s) 350, 805, 1031
AsuII TTCGAA 1 cut(s) 355
AvaII GGWCC 1 cut(s) 1291
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 3 cut(s) 1014, 1057, 1189
BccI CCATC 8 cut(s) 134, 335, 370, 440, 889, 1073, 1261, 1331
BceAI ACGGC 1 cut(s) 522
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciT130I CCWGG 1 cut(s) 120
BciVI GTATCC 1 cut(s) 413
BclI TGATCA 1 cut(s) 1134
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 3 cut(s) 138, 860, 1206
BfaI CTAG 3 cut(s) 279, 462, 722
BfmI CTRYAG 2 cut(s) 1069, 1245
BfoI RGCGCY 1 cut(s) 1225
BfuI GTATCC 1 cut(s) 413
BglII AGATCT 1 cut(s) 1249
BisI GCNGC 3 cut(s) 1003, 1046, 1203
BlsI GCNGC 3 cut(s) 1004, 1047, 1204
Bme1390I CCNGG 2 cut(s) 64, 120
Bme18I GGWCC 1 cut(s) 1291
BmgT120I GGNCC 5 cut(s) 429, 626, 1120, 1237, 1291
BmiI GGNNCC 3 cut(s) 61, 124, 1020
BmrFI CCNGG 2 cut(s) 64, 120
BmrI ACTGGG 1 cut(s) 1278
BmsI GCATC 5 cut(s) 105, 180, 901, 1047, 1069
BmuI ACTGGG 1 cut(s) 1278
BoxI GACNNNNGTC 1 cut(s) 1284
BpiI GAAGAC 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 888
Bpu14I TTCGAA 1 cut(s) 355
BpuEI CTTGAG 1 cut(s) 1253
BpuMI CCSGG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 797
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 436
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 416, 437, 498, 1071, 1288
Bse118I RCCGGY 1 cut(s) 1041
Bse1I ACTGG 5 cut(s) 481, 905, 998, 1220, 1284
Bse3DI GCAATG 1 cut(s) 837
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 5 cut(s) 120, 432, 778, 1060, 1272
BseLI CCNNNNNNNGG 6 cut(s) 19, 416, 437, 498, 1071, 1288
BseMI GCAATG 1 cut(s) 837
BseMII CTCAG 3 cut(s) 5, 865, 1040
BseNI ACTGG 5 cut(s) 481, 905, 998, 1220, 1284
BseRI GAGGAG 1 cut(s) 891
BseXI GCAGC 3 cut(s) 1014, 1057, 1189
BshFI GGCC 7 cut(s) 430, 484, 537, 627, 666, 1121, 1239
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 2 cut(s) 63, 1042
BslI CCNNNNNNNGG 6 cut(s) 19, 416, 437, 498, 1071, 1288
BsmAI GTCTC 3 cut(s) 138, 860, 1206
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 7 cut(s) 430, 484, 537, 627, 666, 1121, 1239
Bso31I GGTCTC 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 4 cut(s) 412, 1134, 1249, 1311
BspANI GGCC 7 cut(s) 430, 484, 537, 627, 666, 1121, 1239
BspCNI CTCAG 3 cut(s) 6, 864, 1041
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 3 cut(s) 61, 124, 1020
BspMAI CTGCAG 1 cut(s) 1249
BspT104I TTCGAA 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 1041
BsrI ACTGG 5 cut(s) 481, 905, 998, 1220, 1284
BssAI RCCGGY 1 cut(s) 1041
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 4 cut(s) 412, 1134, 1249, 1311
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 2 cut(s) 133, 714
Bst6I CTCTTC 1 cut(s) 1255
BstBAI YACGTR 1 cut(s) 797
BstBI TTCGAA 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 202, 553, 1174
BstDEI CTNAG 7 cut(s) 14, 308, 566, 851, 885, 1049, 1301
BstENI CCTNNNNNAGG 1 cut(s) 17
BstF5I GGATG 5 cut(s) 120, 432, 778, 1060, 1272
BstH2I RGCGCY 1 cut(s) 1225
BstHHI GCGC 2 cut(s) 1002, 1224
BstKTI GATC 4 cut(s) 415, 1137, 1252, 1314
BstMAI GTCTC 3 cut(s) 138, 860, 1206
BstMBI GATC 4 cut(s) 412, 1134, 1249, 1311
BstMWI GCNNNNNNNGC 1 cut(s) 1182
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 2 cut(s) 682, 1093
BstPAI GACNNNNGTC 1 cut(s) 1284
BstSCI CCNGG 2 cut(s) 62, 118
BstSFI CTRYAG 2 cut(s) 1069, 1245
BstV1I GCAGC 3 cut(s) 1014, 1057, 1189
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 1249
BstXI CCANNNNNNTGG 1 cut(s) 674
BstYI RGATCY 1 cut(s) 1249
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 7 cut(s) 430, 484, 537, 627, 666, 1121, 1239
BtgZI GCGATG 1 cut(s) 1070
BtsCI GGATG 5 cut(s) 120, 432, 778, 1060, 1272
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 2 cut(s) 991, 1167
Cac8I GCNNGC 3 cut(s) 202, 553, 1174
CciI TCATGA 1 cut(s) 730
CfoI GCGC 2 cut(s) 1002, 1224
Cfr10I RCCGGY 1 cut(s) 1041
Cfr13I GGNCC 5 cut(s) 429, 626, 1120, 1237, 1291
CseI GACGC 1 cut(s) 474
Csp6I GTAC 1 cut(s) 798
CviAII CATG 8 cut(s) 668, 679, 731, 950, 1007, 1090, 1186, 1295
CviQI GTAC 1 cut(s) 798
DdeI CTNAG 7 cut(s) 14, 308, 566, 851, 885, 1049, 1301
DpnI GATC 4 cut(s) 414, 1136, 1251, 1313
DpnII GATC 4 cut(s) 412, 1134, 1249, 1311
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 1255
EarI CTCTTC 1 cut(s) 1255
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 484
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 1291
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 1164
EcoICRI GAGCTC 1 cut(s) 850
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 1237
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 436
EcoT22I ATGCAT 2 cut(s) 1062, 1117
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 8 cut(s) 671, 682, 734, 953, 1010, 1093, 1189, 1298
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 8 cut(s) 667, 678, 730, 949, 1006, 1089, 1185, 1294
FauNDI CATATG 1 cut(s) 1349
FbaI TGATCA 1 cut(s) 1134
FblI GTMKAC 1 cut(s) 1160
Fnu4HI GCNGC 3 cut(s) 1003, 1046, 1203
FokI GGATG 5 cut(s) 127, 419, 785, 1047, 1279
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 3 cut(s) 1003, 1046, 1203
FspBI CTAG 3 cut(s) 279, 462, 722
FspI TGCGCA 1 cut(s) 1001
GlaI GCGC 2 cut(s) 1001, 1223
GluI GCNGC 3 cut(s) 1003, 1046, 1203
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 1225
HaeIII GGCC 7 cut(s) 430, 484, 537, 627, 666, 1121, 1239
HapII CCGG 2 cut(s) 63, 1042
HgaI GACGC 1 cut(s) 474
HhaI GCGC 2 cut(s) 1002, 1224
Hin1II CATG 8 cut(s) 671, 682, 734, 953, 1010, 1093, 1189, 1298
Hin6I GCGC 2 cut(s) 1000, 1222
HinP1I GCGC 2 cut(s) 1000, 1222
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 1 cut(s) 783
HinfI GANTC 5 cut(s) 101, 246, 635, 965, 1157
HpaII CCGG 2 cut(s) 63, 1042
HphI GGTGA 3 cut(s) 350, 805, 1031
Hpy166II GTNNAC 1 cut(s) 1161
Hpy188I TCNGA 6 cut(s) 367, 640, 874, 1134, 1144, 1259
Hpy188III TCNNGA 3 cut(s) 410, 731, 1211
Hpy8I GTNNAC 1 cut(s) 1161
HpyAV CCTTC 5 cut(s) 176, 474, 752, 1139, 1250
HpyCH4III ACNGT 2 cut(s) 133, 714
HpyCH4IV ACGT 2 cut(s) 796, 891
HpyCH4V TGCA 9 cut(s) 204, 239, 551, 688, 791, 1060, 1115, 1205, 1247
HpyF10VI GCNNNNNNNGC 1 cut(s) 1182
HpyF3I CTNAG 7 cut(s) 14, 308, 566, 851, 885, 1049, 1301
HpySE526I ACGT 2 cut(s) 796, 891
Hsp92II CATG 8 cut(s) 671, 682, 734, 953, 1010, 1093, 1189, 1298
HspAI GCGC 2 cut(s) 1000, 1222
Ksp22I TGATCA 1 cut(s) 1134
Kzo9I GATC 4 cut(s) 412, 1134, 1249, 1311
LmnI GCTCC 3 cut(s) 122, 319, 962
Lsp1109I GCAGC 3 cut(s) 1014, 1057, 1189
LweI GCATC 5 cut(s) 105, 180, 901, 1047, 1069
MaeI CTAG 3 cut(s) 279, 462, 722
MaeII ACGT 2 cut(s) 796, 891
MalI GATC 4 cut(s) 414, 1136, 1251, 1313
MboI GATC 4 cut(s) 412, 1134, 1249, 1311
MboII GAAGA 9 cut(s) 79, 241, 244, 501, 662, 713, 1117, 1272, 1328
MfeI CAATTG 1 cut(s) 1359
MflI RGATCY 1 cut(s) 1249
MhlI GDGCHC 1 cut(s) 852
MlyI GAGTC 1 cut(s) 1166
Mph1103I ATGCAT 2 cut(s) 1062, 1117
MseI TTAA 3 cut(s) 87, 192, 830
MslI CAYNNNNRTG 2 cut(s) 672, 683
MspA1I CMGCKG 1 cut(s) 1048
MspI CCGG 2 cut(s) 63, 1042
MspR9I CCNGG 2 cut(s) 64, 120
MunI CAATTG 1 cut(s) 1359
Mva1269I GAATGC 1 cut(s) 616
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 1182
NciI CCSGG 1 cut(s) 64
NdeI CATATG 1 cut(s) 1349
NdeII GATC 4 cut(s) 412, 1134, 1249, 1311
NlaIII CATG 8 cut(s) 671, 682, 734, 953, 1010, 1093, 1189, 1298
NlaIV GGNNCC 3 cut(s) 61, 124, 1020
NsbI TGCGCA 1 cut(s) 1001
NsiI ATGCAT 2 cut(s) 1062, 1117
NspI RCATGY 2 cut(s) 682, 1093
NspV TTCGAA 1 cut(s) 355
PagI TCATGA 1 cut(s) 730
PceI AGGCCT 1 cut(s) 484
PctI GAATGC 1 cut(s) 616
PfeI GAWTC 4 cut(s) 101, 246, 635, 965
PflFI GACNNNGTC 1 cut(s) 468
PkrI GCNGC 3 cut(s) 1004, 1047, 1204
PleI GAGTC 1 cut(s) 1165
PpsI GAGTC 1 cut(s) 1165
Ppu21I YACGTR 1 cut(s) 797
PshAI GACNNNNGTC 1 cut(s) 1284
PshBI ATTAAT 1 cut(s) 192
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 3 cut(s) 61, 124, 1020
PspPI GGNCC 5 cut(s) 429, 626, 1120, 1237, 1291
PsrI GAACNNNNNNTAC 2 cut(s) 1290, 1322
PstI CTGCAG 1 cut(s) 1249
PsuI RGATCY 1 cut(s) 1249
PsyI GACNNNGTC 1 cut(s) 468
PvuII CAGCTG 1 cut(s) 1048
RsaI GTAC 1 cut(s) 799
RsaNI GTAC 1 cut(s) 798
RseI CAYNNNNRTG 2 cut(s) 672, 683
SacI GAGCTC 1 cut(s) 852
SalI GTCGAC 1 cut(s) 1159
SaqAI TTAA 3 cut(s) 87, 192, 830
SatI GCNGC 3 cut(s) 1003, 1046, 1203
Sau3AI GATC 4 cut(s) 412, 1134, 1249, 1311
Sau96I GGNCC 5 cut(s) 429, 626, 1120, 1237, 1291
SchI GAGTC 1 cut(s) 1166
ScrFI CCNGG 2 cut(s) 64, 120
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 5 cut(s) 105, 180, 901, 1047, 1069
SfcI CTRYAG 2 cut(s) 1069, 1245
SfuI TTCGAA 1 cut(s) 355
SinI GGWCC 1 cut(s) 1291
SmiMI CAYNNNNRTG 2 cut(s) 672, 683
SmlI CTYRAG 1 cut(s) 1232
SmoI CTYRAG 1 cut(s) 1232
SseBI AGGCCT 1 cut(s) 484
SspMI CTAG 3 cut(s) 279, 462, 722
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 1 cut(s) 484
StyD4I CCNGG 2 cut(s) 62, 118
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 133, 714
TaiI ACGT 2 cut(s) 799, 894
TaqI TCGA 8 cut(s) 355, 472, 487, 909, 1155, 1160, 1210, 1314
TaqII GACCGA 1 cut(s) 792
TfiI GAWTC 4 cut(s) 101, 246, 635, 965
Tru1I TTAA 3 cut(s) 87, 192, 830
Tru9I TTAA 3 cut(s) 87, 192, 830
TscAI CASTG 2 cut(s) 998, 1174
TseI GCWGC 3 cut(s) 1002, 1045, 1202
TspDTI ATGAA 6 cut(s) 17, 706, 965, 977, 1118, 1311
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 2 cut(s) 998, 1174
Tth111I GACNNNGTC 1 cut(s) 468
VpaK11BI GGWCC 1 cut(s) 1291
VspI ATTAAT 1 cut(s) 192
XagI CCTNNNNNAGG 1 cut(s) 17
XapI RAATTY 3 cut(s) 46, 89, 641
XceI RCATGY 2 cut(s) 682, 1093
XmiI GTMKAC 1 cut(s) 1160
XspI CTAG 3 cut(s) 279, 462, 722
Zsp2I ATGCAT 2 cut(s) 1062, 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.