MD14G1018000.v1.1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000803
Physical Location & Seq
Reverse (-)
1697948 .. 1699789
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1018000.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGTCAGACCCAAAAGCAAAACCCAGAGTGGTGTCCTGCATCGGCGACACCCATGGCTTCCACACCAAGCTCCAGAATCTCTGGTCCAATCTCCAATCCGCCATCGACCCATCAGATTTCTCCACCGCCCTCATCATTTTCCTCGGGGATTACTGCGACCGCGGCCCGGACACCAGGAAAGTCATCGACTTTCTCAGCTCCCTTCCCACCAGATATCCGAATCAGAAGCACTTCTTCCTCTCCGGTAACCACGACCTGGCCATCGCCGCCTTCGTGGGCGTCCTGCCGCCGCCTCCCGACGGGTCGGAGTTTTCCGAGGTGTGGAAGGAGTATGCGGATAGCGAGGAGAGAGAAGGTTGGTTTAAGGGAGATGGGTACGAGAGAATGCACTTGCAGGGCAGGAGATGGGGTGGGAAGATCAAGGCCAAGTTTAATGCAGCAAAAGGGGCGGAGTACAAAGGGTCGATTTATGATGCTGGGCCCACATTTGAATCCTATGGGGTCCCACATGGTTCTGCTGATTTGGTTAAGGCTATTCCTGACAAACACAAGAGGTTTCTTTCTGATATGGTTTGGGTCCATGAAGAGGATAATGTCTGCATAGAGACTGCAGATCGAATCAAACACTGTAGATTGATTGTTGTGCATGCTGGTTTAGAGAAAGATAAAGATGTCAAGCAGCAGTTGGCGTTTTTGAAAGCCAAAGACACCCGGGTGCCTAAGATAGAGGCTCTGAGTGGGAGGAAAAATGTATGGGATATACCAAAGGAAATGGCTTTCCACCTCTTAATATCAGCAATCCTTTCTACTGAAACGTTCGTTGTTTATTGTCCGATGATTTTTGTATTTCTTATGTTCAAACCAACTATTGTCGTAAGTGGTCATCACGCGAAACTTAACATTGAAGGCCTCAAACTGACCATTGATGAAGGTGGTGGTATGGCCGAAAGACCAGTGGCTGCAGTTGTGCTCCCCTCTATGAAAATAGTCCTCGATACAGATGCGTTGACAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

339

Amino Acids

37.73

Weight (kDa)

6.67

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 13 - 114 2.3e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 715
AccB7I CCANNNNNTGG 1 cut(s) 256
AccII CGCG 2 cut(s) 162, 890
AciI CCGC 9 cut(s) 99, 126, 160, 162, 267, 287, 290, 335, 449
AclI AACGTT 1 cut(s) 815
AcoI YGGCCR 2 cut(s) 258, 942
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 377, 455
AfiI CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
AgsI TTSAA 4 cut(s) 491, 697, 859, 905
AjnI CCWGG 2 cut(s) 173, 255
AleI CACNNNNGTG 1 cut(s) 713
AluBI AGCT 2 cut(s) 70, 198
AluI AGCT 2 cut(s) 70, 198
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 1 cut(s) 599
AlwNI CAGNNNCTG 1 cut(s) 959
Ama87I CYCGRG 2 cut(s) 143, 711
AoxI GGCC 6 cut(s) 163, 258, 423, 479, 907, 942
ApaI GGGCCC 1 cut(s) 483
ApeKI GCWGC 3 cut(s) 437, 679, 959
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 6 cut(s) 84, 164, 479, 480, 502, 577
AsuC2I CCSGG 3 cut(s) 167, 712, 713
AvaI CYCGRG 2 cut(s) 143, 711
AvaII GGWCC 3 cut(s) 84, 502, 577
BaeGI GKGCMC 1 cut(s) 483
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 3 cut(s) 449, 691, 946
BccI CCATC 5 cut(s) 110, 118, 269, 365, 399
BcgI CGANNNNNNTGC 2 cut(s) 983, 1017
BciT130I CCWGG 2 cut(s) 175, 257
BcnI CCSGG 3 cut(s) 167, 712, 713
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 1 cut(s) 1015
BfmI CTRYAG 3 cut(s) 609, 628, 960
BisI GCNGC 7 cut(s) 163, 267, 287, 290, 438, 680, 960
BlsI GCNGC 7 cut(s) 164, 268, 288, 291, 439, 681, 961
Bme1390I CCNGG 5 cut(s) 167, 175, 257, 712, 713
Bme18I GGWCC 3 cut(s) 84, 502, 577
BmeT110I CYCGRG 2 cut(s) 143, 711
BmgT120I GGNCC 6 cut(s) 84, 164, 479, 480, 502, 577
BmiI GGNNCC 5 cut(s) 481, 503, 504, 578, 717
BmrFI CCNGG 5 cut(s) 167, 175, 257, 712, 713
BmsI GCATC 3 cut(s) 48, 463, 991
BpmI CTGGAG 1 cut(s) 56
BpuMI CCSGG 3 cut(s) 167, 712, 713
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 5 cut(s) 52, 142, 160, 315, 711
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 394, 424
Bsc4I CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
Bse1I ACTGG 1 cut(s) 953
BseBI CCWGG 2 cut(s) 175, 257
BseDI CCNNGG 5 cut(s) 52, 142, 160, 315, 711
BseLI CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
BseMII CTCAG 2 cut(s) 208, 725
BseNI ACTGG 1 cut(s) 953
BseRI GAGGAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 3 cut(s) 449, 691, 946
BseYI CCCAGC 1 cut(s) 476
Bsh1236I CGCG 2 cut(s) 162, 890
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 6 cut(s) 165, 260, 425, 481, 909, 944
BshNI GGYRCC 1 cut(s) 715
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 2 cut(s) 143, 711
BsiSI CCGG 3 cut(s) 167, 243, 712
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 488
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 6 cut(s) 165, 260, 425, 481, 909, 944
BsoBI CYCGRG 2 cut(s) 143, 711
Bsp120I GGGCCC 1 cut(s) 479
Bsp1286I GDGCHC 2 cut(s) 483, 972
Bsp143I GATC 2 cut(s) 417, 613
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 9 cut(s) 99, 126, 160, 162, 267, 287, 290, 335, 449
BspANI GGCC 6 cut(s) 165, 260, 425, 481, 909, 944
BspCNI CTCAG 2 cut(s) 207, 726
BspFNI CGCG 2 cut(s) 162, 890
BspLI GGNNCC 5 cut(s) 481, 503, 504, 578, 717
BspMAI CTGCAG 2 cut(s) 613, 964
BspT107I GGYRCC 1 cut(s) 715
BsrI ACTGG 1 cut(s) 953
BssECI CCNNGG 5 cut(s) 52, 142, 160, 315, 711
BssMI GATC 2 cut(s) 417, 613
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 2 cut(s) 175, 257
Bst4CI ACNGT 1 cut(s) 629
Bst6I CTCTTC 1 cut(s) 579
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 3 cut(s) 194, 720, 734
BstDSI CCRYGG 2 cut(s) 52, 160
BstEII GGTNACC 1 cut(s) 245
BstFNI CGCG 2 cut(s) 162, 890
BstKTI GATC 2 cut(s) 420, 616
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 2 cut(s) 417, 613
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 162, 266, 446
BstNI CCWGG 2 cut(s) 175, 257
BstNSI RCATGY 1 cut(s) 650
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 5 cut(s) 165, 173, 255, 710, 711
BstSFI CTRYAG 3 cut(s) 609, 628, 960
BstSLI GKGCMC 1 cut(s) 483
BstUI CGCG 2 cut(s) 162, 890
BstV1I GCAGC 3 cut(s) 449, 691, 946
BsuRI GGCC 6 cut(s) 165, 260, 425, 481, 909, 944
BtgI CCRYGG 2 cut(s) 52, 160
BtgZI GCGATG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 625, 960
Cac8I GCNNGC 1 cut(s) 648
CaiI CAGNNNCTG 1 cut(s) 959
Cfr13I GGNCC 6 cut(s) 84, 164, 479, 480, 502, 577
Cfr42I CCGCGG 1 cut(s) 163
Cfr9I CCCGGG 1 cut(s) 711
CseI GACGC 1 cut(s) 268
Csp6I GTAC 2 cut(s) 376, 454
CviAII CATG 4 cut(s) 53, 509, 581, 647
CviQI GTAC 2 cut(s) 376, 454
DdeI CTNAG 3 cut(s) 194, 720, 734
DpnI GATC 2 cut(s) 419, 615
DpnII GATC 2 cut(s) 417, 613
EaeI YGGCCR 2 cut(s) 258, 942
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
EciI GGCGGA 2 cut(s) 88, 464
Eco130I CCWWGG 1 cut(s) 52
Eco147I AGGCCT 1 cut(s) 909
Eco24I GRGCYC 1 cut(s) 483
Eco32I GATATC 1 cut(s) 215
Eco47I GGWCC 3 cut(s) 84, 502, 577
Eco88I CYCGRG 2 cut(s) 143, 711
Eco91I GGTNACC 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 502
EcoO65I GGTNACC 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 173, 255
EcoRV GATATC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 4 cut(s) 56, 512, 584, 650
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 488
FatI CATG 4 cut(s) 52, 508, 580, 646
Fnu4HI GCNGC 7 cut(s) 163, 267, 287, 290, 438, 680, 960
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 7 cut(s) 163, 267, 287, 290, 438, 680, 960
FspBI CTAG 1 cut(s) 1015
GluI GCNGC 7 cut(s) 163, 267, 287, 290, 438, 680, 960
GsaI CCCAGC 1 cut(s) 480
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 6 cut(s) 165, 260, 425, 481, 909, 944
HapII CCGG 3 cut(s) 167, 243, 712
HgaI GACGC 1 cut(s) 268
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 4 cut(s) 56, 512, 584, 650
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 4 cut(s) 76, 220, 491, 618
HpaII CCGG 3 cut(s) 167, 243, 712
Hpy166II GTNNAC 1 cut(s) 1008
Hpy188I TCNGA 9 cut(s) 7, 115, 219, 225, 307, 316, 565, 735, 834
Hpy188III TCNNGA 3 cut(s) 73, 296, 539
Hpy8I GTNNAC 1 cut(s) 1008
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 6 cut(s) 212, 280, 319, 347, 899, 923
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 1 cut(s) 815
HpyCH4V TGCA 8 cut(s) 39, 388, 394, 437, 600, 611, 646, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 162, 266, 446
HpyF3I CTNAG 3 cut(s) 194, 720, 734
HpySE526I ACGT 1 cut(s) 815
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 4 cut(s) 56, 512, 584, 650
KflI GGGWCCC 1 cut(s) 502
KspI CCGCGG 1 cut(s) 163
Kzo9I GATC 2 cut(s) 417, 613
LmnI GCTCC 3 cut(s) 75, 203, 975
Lsp1109I GCAGC 3 cut(s) 449, 691, 946
LweI GCATC 3 cut(s) 48, 463, 991
MaeI CTAG 1 cut(s) 1015
MaeII ACGT 1 cut(s) 815
MaeIII GTNAC 1 cut(s) 245
MalI GATC 2 cut(s) 419, 615
MboI GATC 2 cut(s) 417, 613
MboII GAAGA 3 cut(s) 226, 427, 596
MhlI GDGCHC 2 cut(s) 483, 972
MlsI TGGCCA 1 cut(s) 260
MluNI TGGCCA 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 285
Mox20I TGGCCA 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 260
MseI TTAA 5 cut(s) 363, 432, 528, 788, 897
MslI CAYNNNNRTG 1 cut(s) 713
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 3 cut(s) 167, 243, 712
MspR9I CCNGG 5 cut(s) 167, 175, 257, 712, 713
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 2 cut(s) 175, 257
MvnI CGCG 2 cut(s) 162, 890
MwoI GCNNNNNNNGC 3 cut(s) 162, 266, 446
NciI CCSGG 3 cut(s) 167, 712, 713
NcoI CCATGG 1 cut(s) 52
NdeII GATC 2 cut(s) 417, 613
NlaIII CATG 4 cut(s) 56, 512, 584, 650
NlaIV GGNNCC 5 cut(s) 481, 503, 504, 578, 717
NspI RCATGY 1 cut(s) 650
OliI CACNNNNGTG 1 cut(s) 713
PaeI GCATGC 1 cut(s) 650
PceI AGGCCT 1 cut(s) 909
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 4 cut(s) 76, 220, 491, 618
PflMI CCANNNNNTGG 1 cut(s) 256
PkrI GCNGC 7 cut(s) 164, 268, 288, 291, 439, 681, 961
PpuMI RGGWCCY 1 cut(s) 502
Psp1406I AACGTT 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 173, 255
PspEI GGTNACC 1 cut(s) 245
PspFI CCCAGC 1 cut(s) 476
PspGI CCWGG 2 cut(s) 173, 255
PspN4I GGNNCC 5 cut(s) 481, 503, 504, 578, 717
PspOMI GGGCCC 1 cut(s) 479
PspPI GGNCC 6 cut(s) 84, 164, 479, 480, 502, 577
PspPPI RGGWCCY 1 cut(s) 502
PstI CTGCAG 2 cut(s) 613, 964
PstNI CAGNNNCTG 1 cut(s) 959
RsaI GTAC 2 cut(s) 377, 455
RsaNI GTAC 2 cut(s) 376, 454
RseI CAYNNNNRTG 1 cut(s) 713
SacII CCGCGG 1 cut(s) 163
SaqAI TTAA 5 cut(s) 363, 432, 528, 788, 897
SatI GCNGC 7 cut(s) 163, 267, 287, 290, 438, 680, 960
Sau3AI GATC 2 cut(s) 417, 613
Sau96I GGNCC 6 cut(s) 84, 164, 479, 480, 502, 577
ScrFI CCNGG 5 cut(s) 167, 175, 257, 712, 713
SduI GDGCHC 2 cut(s) 483, 972
SetI ASST 9 cut(s) 72, 200, 258, 321, 358, 557, 786, 818, 934
SfaNI GCATC 3 cut(s) 48, 463, 991
SfcI CTRYAG 3 cut(s) 609, 628, 960
Sfr303I CCGCGG 1 cut(s) 163
SgrBI CCGCGG 1 cut(s) 163
SinI GGWCC 3 cut(s) 84, 502, 577
SmaI CCCGGG 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 713
SphI GCATGC 1 cut(s) 650
SseBI AGGCCT 1 cut(s) 909
SsiI CCGC 9 cut(s) 99, 126, 160, 162, 267, 287, 290, 335, 449
SspMI CTAG 1 cut(s) 1015
StuI AGGCCT 1 cut(s) 909
StyD4I CCNGG 5 cut(s) 165, 173, 255, 710, 711
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 1 cut(s) 818
TaqI TCGA 5 cut(s) 105, 186, 464, 616, 993
TatI WGTACW 1 cut(s) 453
TauI GCSGC 4 cut(s) 165, 269, 289, 292
TfiI GAWTC 4 cut(s) 76, 220, 491, 618
Tru1I TTAA 5 cut(s) 363, 432, 528, 788, 897
Tru9I TTAA 5 cut(s) 363, 432, 528, 788, 897
TscAI CASTG 2 cut(s) 632, 960
TseI GCWGC 3 cut(s) 437, 679, 959
TspDTI ATGAA 3 cut(s) 597, 942, 995
TspMI CCCGGG 1 cut(s) 711
TspRI CASTG 2 cut(s) 632, 960
Van91I CCANNNNNTGG 1 cut(s) 256
VpaK11BI GGWCC 3 cut(s) 84, 502, 577
XceI RCATGY 1 cut(s) 650
XmaI CCCGGG 1 cut(s) 711
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.