RchiOBHm_Chr3g0472441

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000803
Physical Location & Seq
Reverse (-)
18343037 .. 18346197
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43815

Sequence Viewer

Length: 990 bp
ATGATTGTAGCAACTTTCCACATTCGTTTAACCAAAGTCCTTCACCGGAAGACCAAAACCATGTCAGACCCAAAACCCAGACTAGTCTGCTGCATCGGCGACGTCCACGGCTTCCACACGAAGCTCCAAAACCTCTGGTCCAATCTGGAAATCTCCATCCCCTCTTCAGAATTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGCGACCGCGGCCCAGACACCAACAAAGTCCTCGACTTCCTCATTTCCCTGCCCTCCAGATACCCGCATCAGAAACACGTCTTCATCGCGGGAAACCACGACTTCGCCTTCGCCGGGTTCTTGAGAGTCCTGCCTCCGCCGGCGGACGGGTCCGAGTTCCGGGAGGGGTGGAAGGAGTTCGAGGAGAGTGAGGAGAGAGAAGGGTGGTTTAAGGGCGAGGGGTACGAGGGAATGCACTTGCAGGGGAGGAGATGGGCTGGGAGTAGTAAGGGGTTTAATAAGGCTAAAGGGACTGAGTACCAGGGTTCCGTTTATAAAGCTGGGCCCACTTTTGAGTCTTATGGAGTGCCTCATGGCTCTGCCGATCTGGTGAAGGTAGTTCCTGATGATCATAAGAAGTTTCTTGCCGATATGGTTTGGGTGCATGAAGAGGATGATGTCTGTGTAGAGATTGAGGGTGAAACCAAACACTGTAAATTGATTGCTGTGCACGCCGGTTTGGAGAAAGATAACGATGTCAAACAACAGTTGGAGTTTCTGAAAGCCAGAGATACTCGGGTACCTAAGATAAAGGCTCTGAGTGGCAGGAAAGACGTGTTGAATATACCAGAGGAACTGAAAAAGACTCAAACTGTTATTGTAAGTGGACATCATGGGAAACTTCATATTGAAGGCTTAAGGCTGATTATTGATGAAGGCGATGGTAAGGCAAAGAACCCAGTGGCTGCAATTTTACTCCCCTCTATGAAAATAATCCGTGATACAGACGTTTTGGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

329

Amino Acids

36.9

Weight (kDa)

6.77

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 29 - 130 2.5e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AatII GACGTC 1 cut(s) 105
Acc65I GGTACC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 766
AccII CGCG 2 cut(s) 216, 296
AciI CCGC 7 cut(s) 180, 214, 216, 272, 296, 344, 350
AcsI RAATTY 1 cut(s) 170
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 3 cut(s) 431, 506, 768
AfiI CCNNNNNNNGG 3 cut(s) 321, 353, 366
AflII CTTAAG 1 cut(s) 883
AflIII ACRYGT 2 cut(s) 283, 801
AgsI TTSAA 2 cut(s) 808, 878
AhlI ACTAGT 1 cut(s) 82
AjiI CACGTC 2 cut(s) 286, 802
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 2 cut(s) 124, 527
AluI AGCT 2 cut(s) 124, 527
Alw21I GWGCWC 1 cut(s) 699
Alw44I GTGCAC 1 cut(s) 695
AlwNI CAGNNNCTG 1 cut(s) 932
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 2 cut(s) 217, 530
ApaI GGGCCC 1 cut(s) 534
ApaLI GTGCAC 1 cut(s) 695
ApeKI GCWGC 2 cut(s) 90, 932
ApoI RAATTY 1 cut(s) 170
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 383
Asp718I GGTACC 1 cut(s) 766
AspS9I GGNCC 5 cut(s) 138, 218, 357, 530, 531
AsuC2I CCSGG 2 cut(s) 322, 368
AsuHPI GGTGA 3 cut(s) 35, 589, 677
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 2 cut(s) 138, 357
BaeGI GKGCMC 2 cut(s) 534, 699
BaeI ACNNNNGTAYC 5 cut(s) 750, 750, 783, 783, 960
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 534
BbsI GAAGAC 2 cut(s) 56, 280
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 2 cut(s) 77, 919
BccI CCATC 3 cut(s) 164, 453, 902
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 2 cut(s) 322, 368
BcuI ACTAGT 1 cut(s) 82
BfaI CTAG 2 cut(s) 83, 988
BfrI CTTAAG 1 cut(s) 883
BisI GCNGC 3 cut(s) 91, 217, 933
BlsI GCNGC 3 cut(s) 92, 218, 934
Bme1390I CCNGG 3 cut(s) 322, 368, 509
Bme18I GGWCC 2 cut(s) 138, 357
BmeT110I CYCGRG 1 cut(s) 762
BmgBI CACGTC 2 cut(s) 286, 802
BmgT120I GGNCC 5 cut(s) 138, 218, 357, 530, 531
BmiI GGNNCC 4 cut(s) 358, 514, 532, 768
BmrFI CCNGG 3 cut(s) 322, 368, 509
BmrI ACTGGG 1 cut(s) 920
BmsI GCATC 2 cut(s) 102, 283
BmuI ACTGGG 1 cut(s) 920
BpiI GAAGAC 2 cut(s) 56, 280
BpmI CTGGAG 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 349
BpuMI CCSGG 2 cut(s) 322, 368
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 106, 196, 214, 508
BsaWI WCCGGW 1 cut(s) 45
Bsc4I CCNNNNNNNGG 3 cut(s) 321, 353, 366
Bse118I RCCGGY 2 cut(s) 346, 701
Bse1I ACTGG 1 cut(s) 926
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 4 cut(s) 106, 196, 214, 508
BseGI GGATG 2 cut(s) 156, 646
BseLI CCNNNNNNNGG 3 cut(s) 321, 353, 366
BseMII CTCAG 2 cut(s) 492, 776
BseNI ACTGG 1 cut(s) 926
BseRI GAGGAG 3 cut(s) 404, 413, 469
BseSI GKGCMC 2 cut(s) 534, 699
BseXI GCAGC 2 cut(s) 77, 919
BseYI CCCAGC 2 cut(s) 464, 527
Bsh1236I CGCG 2 cut(s) 216, 296
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 219, 532
BshNI GGYRCC 1 cut(s) 766
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 5 cut(s) 46, 321, 347, 367, 702
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 3 cut(s) 321, 353, 366
BsmFI GGGAC 1 cut(s) 511
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 2 cut(s) 219, 532
BsoBI CYCGRG 1 cut(s) 762
Bsp120I GGGCCC 1 cut(s) 530
Bsp1286I GDGCHC 2 cut(s) 534, 699
Bsp143I GATC 2 cut(s) 571, 595
BspACI CCGC 7 cut(s) 180, 214, 216, 272, 296, 344, 350
BspANI GGCC 2 cut(s) 219, 532
BspCNI CTCAG 2 cut(s) 493, 777
BspFNI CGCG 2 cut(s) 216, 296
BspLI GGNNCC 4 cut(s) 358, 514, 532, 768
BspT107I GGYRCC 1 cut(s) 766
BspTI CTTAAG 1 cut(s) 883
BsrFI RCCGGY 2 cut(s) 346, 701
BsrI ACTGG 1 cut(s) 926
BssAI RCCGGY 2 cut(s) 346, 701
BssECI CCNNGG 4 cut(s) 106, 196, 214, 508
BssMI GATC 2 cut(s) 571, 595
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 680, 735, 841
Bst6I CTCTTC 2 cut(s) 169, 630
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 883
BstC8I GCNNGC 2 cut(s) 348, 699
BstDEI CTNAG 3 cut(s) 501, 771, 785
BstDSI CCRYGG 2 cut(s) 106, 214
BstF5I GGATG 2 cut(s) 156, 646
BstFNI CGCG 2 cut(s) 216, 296
BstKTI GATC 2 cut(s) 574, 598
BstMBI GATC 2 cut(s) 571, 595
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 4 cut(s) 96, 207, 216, 698
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 3 cut(s) 320, 366, 507
BstSLI GKGCMC 2 cut(s) 534, 699
BstUI CGCG 2 cut(s) 216, 296
BstV1I GCAGC 2 cut(s) 77, 919
BstV2I GAAGAC 2 cut(s) 56, 280
BsuRI GGCC 2 cut(s) 219, 532
BtgI CCRYGG 2 cut(s) 106, 214
BtgZI GCGATG 2 cut(s) 277, 921
BtrI CACGTC 2 cut(s) 286, 802
BtsCI GGATG 2 cut(s) 156, 646
BtsIMutI CAGTG 2 cut(s) 676, 933
Cac8I GCNNGC 2 cut(s) 348, 699
CaiI CAGNNNCTG 1 cut(s) 932
Cfr10I RCCGGY 2 cut(s) 346, 701
Cfr13I GGNCC 5 cut(s) 138, 218, 357, 530, 531
Cfr42I CCGCGG 1 cut(s) 217
Csp6I GTAC 3 cut(s) 430, 505, 767
CviAII CATG 4 cut(s) 61, 560, 632, 860
CviQI GTAC 3 cut(s) 430, 505, 767
DdeI CTNAG 3 cut(s) 501, 771, 785
DpnI GATC 2 cut(s) 573, 597
DpnII GATC 2 cut(s) 571, 595
Eam1104I CTCTTC 2 cut(s) 169, 630
EarI CTCTTC 2 cut(s) 169, 630
EciI GGCGGA 2 cut(s) 333, 365
Eco24I GRGCYC 1 cut(s) 534
Eco47I GGWCC 2 cut(s) 138, 357
Eco57I CTGAAG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 762
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 534
FaeI CATG 4 cut(s) 64, 563, 635, 863
FaqI GGGAC 1 cut(s) 511
FatI CATG 4 cut(s) 60, 559, 631, 859
FauI CCCGC 2 cut(s) 279, 289
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 3 cut(s) 91, 217, 933
FokI GGATG 2 cut(s) 143, 653
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 3 cut(s) 91, 217, 933
FspBI CTAG 2 cut(s) 83, 988
GluI GCNGC 3 cut(s) 91, 217, 933
GsaI CCCAGC 2 cut(s) 468, 531
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 2 cut(s) 219, 532
HapII CCGG 5 cut(s) 46, 321, 347, 367, 702
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 4 cut(s) 64, 563, 635, 863
HinfI GANTC 3 cut(s) 333, 542, 832
HpaII CCGG 5 cut(s) 46, 321, 347, 367, 702
HphI GGTGA 3 cut(s) 35, 589, 677
Hpy166II GTNNAC 3 cut(s) 106, 697, 854
Hpy188I TCNGA 6 cut(s) 67, 169, 279, 361, 747, 786
Hpy188III TCNNGA 4 cut(s) 146, 264, 328, 590
Hpy8I GTNNAC 3 cut(s) 106, 697, 854
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 7 cut(s) 50, 325, 373, 401, 574, 872, 896
HpyCH4III ACNGT 3 cut(s) 680, 735, 841
HpyCH4IV ACGT 4 cut(s) 102, 285, 801, 975
HpyCH4V TGCA 6 cut(s) 93, 442, 448, 631, 697, 935
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 207, 216, 698
HpyF3I CTNAG 3 cut(s) 501, 771, 785
HpySE526I ACGT 4 cut(s) 102, 285, 801, 975
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 4 cut(s) 64, 563, 635, 863
KpnI GGTACC 1 cut(s) 770
KroI GCCGGC 1 cut(s) 346
KroNI GCCGGC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 595
KspI CCGCGG 1 cut(s) 217
Kzo9I GATC 2 cut(s) 571, 595
LmnI GCTCC 1 cut(s) 129
Lsp1109I GCAGC 2 cut(s) 77, 919
LweI GCATC 2 cut(s) 102, 283
MaeI CTAG 2 cut(s) 83, 988
MaeII ACGT 4 cut(s) 102, 285, 801, 975
MalI GATC 2 cut(s) 573, 597
MboI GATC 2 cut(s) 571, 595
MboII GAAGA 5 cut(s) 61, 156, 184, 280, 647
MhlI GDGCHC 2 cut(s) 534, 699
MluCI AATT 3 cut(s) 170, 683, 936
MlyI GAGTC 3 cut(s) 342, 551, 826
MmeI TCCRAC 1 cut(s) 717
MreI CGCCGGCG 1 cut(s) 346
MroNI GCCGGC 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 4 cut(s) 29, 417, 483, 884
MspA1I CMGCKG 1 cut(s) 216
MspCI CTTAAG 1 cut(s) 883
MspI CCGG 5 cut(s) 46, 321, 347, 367, 702
MspR9I CCNGG 3 cut(s) 322, 368, 509
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 2 cut(s) 216, 296
MwoI GCNNNNNNNGC 4 cut(s) 96, 207, 216, 698
NaeI GCCGGC 1 cut(s) 348
NciI CCSGG 2 cut(s) 322, 368
NdeII GATC 2 cut(s) 571, 595
NgoMIV GCCGGC 1 cut(s) 346
NlaIII CATG 4 cut(s) 64, 563, 635, 863
NlaIV GGNNCC 4 cut(s) 358, 514, 532, 768
NmeAIII GCCGAG 1 cut(s) 177
PctI GAATGC 1 cut(s) 444
PdiI GCCGGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 383
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 3 cut(s) 92, 218, 934
PleI GAGTC 3 cut(s) 341, 550, 826
PpsI GAGTC 3 cut(s) 341, 550, 826
PsiI TTATAA 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 507
PspFI CCCAGC 2 cut(s) 464, 527
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 4 cut(s) 358, 514, 532, 768
PspOMI GGGCCC 1 cut(s) 530
PspPI GGNCC 5 cut(s) 138, 218, 357, 530, 531
PstNI CAGNNNCTG 1 cut(s) 932
RsaI GTAC 3 cut(s) 431, 506, 768
RsaNI GTAC 3 cut(s) 430, 505, 767
SacII CCGCGG 1 cut(s) 217
SaqAI TTAA 4 cut(s) 29, 417, 483, 884
SatI GCNGC 3 cut(s) 91, 217, 933
Sau3AI GATC 2 cut(s) 571, 595
Sau96I GGNCC 5 cut(s) 138, 218, 357, 530, 531
SchI GAGTC 3 cut(s) 342, 551, 826
ScrFI CCNGG 3 cut(s) 322, 368, 509
SduI GDGCHC 2 cut(s) 534, 699
SetI ASST 9 cut(s) 105, 126, 135, 288, 529, 585, 772, 804, 978
SfaNI GCATC 2 cut(s) 102, 283
Sfr303I CCGCGG 1 cut(s) 217
SgrAI CRCCGGYG 1 cut(s) 346
SgrBI CCGCGG 1 cut(s) 217
SinI GGWCC 2 cut(s) 138, 357
SmlI CTYRAG 2 cut(s) 328, 883
SmoI CTYRAG 2 cut(s) 328, 883
SpeI ACTAGT 1 cut(s) 82
Sse9I AATT 3 cut(s) 170, 683, 936
SsiI CCGC 7 cut(s) 180, 214, 216, 272, 296, 344, 350
SspMI CTAG 2 cut(s) 83, 988
StyD4I CCNGG 3 cut(s) 320, 366, 507
TaaI ACNGT 3 cut(s) 680, 735, 841
TaiI ACGT 4 cut(s) 105, 288, 804, 978
TaqI TCGA 2 cut(s) 240, 387
TasI AATT 3 cut(s) 170, 683, 936
TauI GCSGC 1 cut(s) 219
Tru1I TTAA 4 cut(s) 29, 417, 483, 884
Tru9I TTAA 4 cut(s) 29, 417, 483, 884
TscAI CASTG 2 cut(s) 683, 933
TseI GCWGC 2 cut(s) 90, 932
TspDTI ATGAA 5 cut(s) 280, 648, 860, 915, 968
TspGWI ACGGA 2 cut(s) 505, 953
TspRI CASTG 2 cut(s) 683, 933
Vha464I CTTAAG 1 cut(s) 883
VneI GTGCAC 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 138, 357
XapI RAATTY 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 2 cut(s) 83, 988
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.