Rmu_sc0018288.1_g000003

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018288.1
OG0000803
Physical Location & Seq
Forward (+)
18301 .. 20577
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018288.1_g000003.1.cds

Sequence Viewer

Length: 936 bp
atgtcggacccaaaacccagactagtctgctgcatcggcgacgtccacggcttccacacgaagctccaaaacctctggtccaatctcgaatcctccatcccctcttcagacttcgccaccgccctcgtcatcttcctcggcgactactgcgaccgcggcccggacaccagcaaagtcctcgacttcctcatcgccctcccctccagatacccgcatcagaaacacgtcttcatcgcgggaaaccacgacttcgccttcgccggcttcttgagagtcctgcctccgccgccggacgggtccgagttccgggaggggtggaaggagttcgaggacaatgaggagagagaagggtggttcaagggcgaggggtacgagggaatgcaattgcaggggaggagatgggctgggagtatgaaggggtttaacaaggctaaggggactgattaccagggttccatttatgacgctgcgcccacttttgagtcttatggggtgcctcatggctctgccgatctggtgaaggcagttcctgatgatcataagaagtttcttgccgatatggtttgggtgcatgaagaggatgatgtctgtgtagagattgagggtgaaaccaaacactgtaaattgattgccgtgcacgccggtttggagaaagatatagatgtcaaacaacagttggagtttctgaaagccagagatactcgggtacctaagataaaggctctgagtggcaggaaagacgtgtggaatataccagaggtgatggaactgaaaaagactcatactgttattgtaagtggacatcatgggaaacttcatattgaaggcttaaggctgattattgatgaaggcggtggtatggcaaagaacccggtggctgcaattttactcccctctatgaggatagtccgtgatacagacattttggcaaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

311

Amino Acids

34.77

Weight (kDa)

5.87

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 2 cut(s) 493, 706
AccII CGCG 2 cut(s) 156, 236
AciI CCGC 8 cut(s) 120, 154, 156, 212, 236, 284, 287, 852
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 2 cut(s) 371, 708
AfiI CCNNNNNNNGG 4 cut(s) 160, 293, 306, 900
AflII CTTAAG 1 cut(s) 829
AflIII ACRYGT 2 cut(s) 223, 741
AgsI TTSAA 2 cut(s) 358, 824
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 2 cut(s) 226, 742
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw21I GWGCWC 1 cut(s) 639
Alw44I GTGCAC 1 cut(s) 635
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 157
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 3 cut(s) 30, 467, 878
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 1 cut(s) 323
Asp718I GGTACC 1 cut(s) 706
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 4 cut(s) 7, 78, 158, 297
AsuC2I CCSGG 3 cut(s) 161, 308, 872
AsuHPI GGTGA 3 cut(s) 529, 617, 772
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 3 cut(s) 7, 78, 297
BaeGI GKGCMC 1 cut(s) 639
BaeI ACNNNNGTAYC 4 cut(s) 690, 690, 723, 723
BanI GGYRCC 2 cut(s) 493, 706
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 3 cut(s) 17, 454, 865
BccI CCATC 3 cut(s) 104, 393, 757
BceAI ACGGC 2 cut(s) 64, 617
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 3 cut(s) 161, 308, 872
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 934
BfrI CTTAAG 1 cut(s) 829
BisI GCNGC 5 cut(s) 31, 157, 287, 468, 879
BlsI GCNGC 5 cut(s) 32, 158, 288, 469, 880
Bme1390I CCNGG 4 cut(s) 161, 308, 449, 872
Bme18I GGWCC 3 cut(s) 7, 78, 297
BmeT110I CYCGRG 1 cut(s) 702
BmgBI CACGTC 2 cut(s) 226, 742
BmgT120I GGNCC 4 cut(s) 7, 78, 158, 297
BmiI GGNNCC 5 cut(s) 9, 298, 454, 495, 708
BmrFI CCNGG 4 cut(s) 161, 308, 449, 872
BmsI GCATC 2 cut(s) 42, 223
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 3 cut(s) 161, 308, 872
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 46, 136, 154, 448
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 293, 306, 900
Bse118I RCCGGY 2 cut(s) 260, 641
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 4 cut(s) 46, 136, 154, 448
BseGI GGATG 2 cut(s) 96, 586
BseLI CCNNNNNNNGG 4 cut(s) 160, 293, 306, 900
BseMII CTCAG 1 cut(s) 716
BseRI GAGGAG 2 cut(s) 353, 409
BseSI GKGCMC 1 cut(s) 639
BseXI GCAGC 3 cut(s) 17, 454, 865
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 2 cut(s) 156, 236
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 159
BshNI GGYRCC 2 cut(s) 493, 706
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 6 cut(s) 161, 261, 290, 307, 642, 872
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 4 cut(s) 160, 293, 306, 900
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 2 cut(s) 511, 535
BspACI CCGC 8 cut(s) 120, 154, 156, 212, 236, 284, 287, 852
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 717
BspFNI CGCG 2 cut(s) 156, 236
BspLI GGNNCC 5 cut(s) 9, 298, 454, 495, 708
BspT107I GGYRCC 2 cut(s) 493, 706
BspTI CTTAAG 1 cut(s) 829
BsrFI RCCGGY 2 cut(s) 260, 641
BssAI RCCGGY 2 cut(s) 260, 641
BssECI CCNNGG 4 cut(s) 46, 136, 154, 448
BssMI GATC 2 cut(s) 511, 535
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 620, 675, 787
Bst6I CTCTTC 2 cut(s) 109, 570
BstACI GRCGYC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 829
BstC8I GCNNGC 2 cut(s) 262, 639
BstDEI CTNAG 3 cut(s) 432, 711, 725
BstDSI CCRYGG 2 cut(s) 46, 154
BstENI CCTNNNNNAGG 1 cut(s) 898
BstF5I GGATG 2 cut(s) 96, 586
BstFNI CGCG 2 cut(s) 156, 236
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 2 cut(s) 514, 538
BstMBI GATC 2 cut(s) 511, 535
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 4 cut(s) 159, 306, 447, 870
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 2 cut(s) 156, 236
BstV1I GCAGC 3 cut(s) 17, 454, 865
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 159
BtgI CCRYGG 2 cut(s) 46, 154
BtgZI GCGATG 2 cut(s) 175, 217
BtrI CACGTC 2 cut(s) 226, 742
BtsCI GGATG 2 cut(s) 96, 586
BtsIMutI CAGTG 1 cut(s) 616
Cac8I GCNNGC 2 cut(s) 262, 639
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 2 cut(s) 260, 641
Cfr13I GGNCC 4 cut(s) 7, 78, 158, 297
Cfr42I CCGCGG 1 cut(s) 157
CseI GACGC 1 cut(s) 473
Csp6I GTAC 2 cut(s) 370, 707
CviAII CATG 3 cut(s) 500, 572, 806
CviQI GTAC 2 cut(s) 370, 707
DdeI CTNAG 3 cut(s) 432, 711, 725
DpnI GATC 2 cut(s) 513, 537
DpnII GATC 2 cut(s) 511, 535
Eam1104I CTCTTC 2 cut(s) 109, 570
EarI CTCTTC 2 cut(s) 109, 570
EciI GGCGGA 1 cut(s) 273
Eco47I GGWCC 3 cut(s) 7, 78, 297
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 702
EcoNI CCTNNNNNAGG 1 cut(s) 898
EcoRII CCWGG 1 cut(s) 447
FaeI CATG 3 cut(s) 503, 575, 809
FaqI GGGAC 1 cut(s) 451
FatI CATG 3 cut(s) 499, 571, 805
FauI CCCGC 2 cut(s) 219, 229
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 5 cut(s) 31, 157, 287, 468, 879
FokI GGATG 2 cut(s) 83, 593
Fsp4HI GCNGC 5 cut(s) 31, 157, 287, 468, 879
FspBI CTAG 2 cut(s) 23, 934
GlaI GCGC 1 cut(s) 471
GluI GCNGC 5 cut(s) 31, 157, 287, 468, 879
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 1 cut(s) 159
HapII CCGG 6 cut(s) 161, 261, 290, 307, 642, 872
HgaI GACGC 1 cut(s) 473
HhaI GCGC 1 cut(s) 472
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 3 cut(s) 503, 575, 809
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HinfI GANTC 4 cut(s) 89, 273, 482, 778
HpaII CCGG 6 cut(s) 161, 261, 290, 307, 642, 872
HphI GGTGA 3 cut(s) 529, 617, 772
Hpy166II GTNNAC 3 cut(s) 46, 637, 800
Hpy188I TCNGA 6 cut(s) 7, 109, 219, 301, 687, 726
Hpy188III TCNNGA 4 cut(s) 86, 204, 268, 530
Hpy8I GTNNAC 3 cut(s) 46, 637, 800
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 7 cut(s) 265, 313, 341, 409, 514, 818, 842
HpyCH4III ACNGT 3 cut(s) 620, 675, 787
HpyCH4IV ACGT 3 cut(s) 42, 225, 741
HpyCH4V TGCA 6 cut(s) 33, 382, 388, 571, 637, 881
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
HpyF3I CTNAG 3 cut(s) 432, 711, 725
HpySE526I ACGT 3 cut(s) 42, 225, 741
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 3 cut(s) 503, 575, 809
HspAI GCGC 1 cut(s) 470
KpnI GGTACC 1 cut(s) 710
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 535
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 2 cut(s) 511, 535
LmnI GCTCC 1 cut(s) 69
Lsp1109I GCAGC 3 cut(s) 17, 454, 865
LweI GCATC 2 cut(s) 42, 223
MaeI CTAG 2 cut(s) 23, 934
MaeII ACGT 3 cut(s) 42, 225, 741
MalI GATC 2 cut(s) 513, 537
MboI GATC 2 cut(s) 511, 535
MboII GAAGA 4 cut(s) 96, 124, 220, 587
MfeI CAATTG 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 3 cut(s) 383, 623, 882
MlyI GAGTC 3 cut(s) 282, 491, 772
MmeI TCCRAC 1 cut(s) 657
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 2 cut(s) 423, 830
MspA1I CMGCKG 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 829
MspI CCGG 6 cut(s) 161, 261, 290, 307, 642, 872
MspR9I CCNGG 4 cut(s) 161, 308, 449, 872
MunI CAATTG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 2 cut(s) 156, 236
MwoI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 3 cut(s) 161, 308, 872
NdeII GATC 2 cut(s) 511, 535
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 3 cut(s) 503, 575, 809
NlaIV GGNNCC 5 cut(s) 9, 298, 454, 495, 708
NmeAIII GCCGAG 1 cut(s) 117
PctI GAATGC 1 cut(s) 384
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 5 cut(s) 32, 158, 288, 469, 880
PleI GAGTC 3 cut(s) 281, 490, 772
PpsI GAGTC 3 cut(s) 281, 490, 772
Psp6I CCWGG 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 5 cut(s) 9, 298, 454, 495, 708
PspPI GGNCC 4 cut(s) 7, 78, 158, 297
PstNI CAGNNNCTG 1 cut(s) 530
RsaI GTAC 2 cut(s) 371, 708
RsaNI GTAC 2 cut(s) 370, 707
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 2 cut(s) 423, 830
SatI GCNGC 5 cut(s) 31, 157, 287, 468, 879
Sau3AI GATC 2 cut(s) 511, 535
Sau96I GGNCC 4 cut(s) 7, 78, 158, 297
SchI GAGTC 3 cut(s) 282, 491, 772
ScrFI CCNGG 4 cut(s) 161, 308, 449, 872
SduI GDGCHC 1 cut(s) 639
SetI ASST 7 cut(s) 45, 66, 75, 228, 712, 744, 762
SfaNI GCATC 2 cut(s) 42, 223
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 3 cut(s) 7, 78, 297
SmlI CTYRAG 2 cut(s) 268, 829
SmoI CTYRAG 2 cut(s) 268, 829
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 3 cut(s) 383, 623, 882
SsiI CCGC 8 cut(s) 120, 154, 156, 212, 236, 284, 287, 852
SspMI CTAG 2 cut(s) 23, 934
StyD4I CCNGG 4 cut(s) 159, 306, 447, 870
TaaI ACNGT 3 cut(s) 620, 675, 787
TaiI ACGT 3 cut(s) 45, 228, 744
TaqI TCGA 3 cut(s) 87, 180, 327
TasI AATT 3 cut(s) 383, 623, 882
TauI GCSGC 2 cut(s) 159, 289
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 423, 830
Tru9I TTAA 2 cut(s) 423, 830
TscAI CASTG 1 cut(s) 623
TseI GCWGC 3 cut(s) 30, 467, 878
TspDTI ATGAA 5 cut(s) 220, 428, 588, 806, 861
TspGWI ACGGA 1 cut(s) 899
TspRI CASTG 1 cut(s) 623
Vha464I CTTAAG 1 cut(s) 829
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 3 cut(s) 7, 78, 297
XagI CCTNNNNNAGG 1 cut(s) 898
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 2 cut(s) 23, 934
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.