Rmu_sc0003363.1_g000076

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003363.1
OG0000803
Physical Location & Seq
Forward (+)
377350 .. 381465
4116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003363.1_g000076.1.cds

Sequence Viewer

Length: 504 bp
atgtcagacccaaaacccagactagtctgttgcatcggtgacgtccatggcttccacaccaagctccaaaatctctggtccaatctcgaaatctccattccttcttcagacttagccaccgccctcgtcaacttccttggtgactactgcggtcgcggcccagacaccaacgaagtccttgacttcttcgtcgctctcccctccatatacccacgtcagaaagatgtcttcatcgccggaaaccacgacttcgccttcgccgccttcttgagagtcctggctccgccgccggatgggtccgagttctgcaagggatctctggcgagcgaggagtaccagatccgccgattcttgcctgaaagcttttatccatctcaagaaccacatccgcctcaagaccgccgatcccacgcccaaatcattggattcttcgacctcgtcgccgaaaccggaatcagaccacacagctctatctctggaatcgaaaccggaatcctccagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

167

Amino Acids

18.53

Weight (kDa)

5.24

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AatII GACGTC 1 cut(s) 45
AccII CGCG 1 cut(s) 156
AciI CCGC 9 cut(s) 120, 150, 156, 261, 284, 287, 343, 389, 400
AclWI GGATC 3 cut(s) 322, 334, 399
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 293
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 3 cut(s) 64, 363, 468
AluI AGCT 3 cut(s) 64, 363, 468
AlwI GGATC 3 cut(s) 322, 334, 399
AoxI GGCC 1 cut(s) 157
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AspS9I GGNCC 3 cut(s) 78, 158, 297
AsuHPI GGTGA 2 cut(s) 50, 152
AvaII GGWCC 2 cut(s) 78, 297
BbsI GAAGAC 1 cut(s) 220
BccI CCATC 2 cut(s) 287, 379
BciT130I CCWGG 1 cut(s) 278
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 1 cut(s) 23
BisI GCNGC 3 cut(s) 157, 261, 287
BlsI GCNGC 3 cut(s) 158, 262, 288
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 2 cut(s) 78, 297
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 3 cut(s) 78, 158, 297
BmiI GGNNCC 2 cut(s) 282, 298
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 482
BpuEI CTTGAG 3 cut(s) 289, 360, 378
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 46, 136
BsaWI WCCGGW 2 cut(s) 449, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 499
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 2 cut(s) 46, 136
BseGI GGATG 2 cut(s) 298, 385
BseLI CCNNNNNNNGG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 499
BseRI GAGGAG 1 cut(s) 344
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 4 cut(s) 237, 290, 450, 489
BslI CCNNNNNNNGG 1 cut(s) 293
BsnI GGCC 1 cut(s) 159
Bsp143I GATC 3 cut(s) 314, 339, 404
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 9 cut(s) 120, 150, 156, 261, 284, 287, 343, 389, 400
BspANI GGCC 1 cut(s) 159
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 282, 298
BspPI GGATC 3 cut(s) 322, 334, 399
BsrI ACTGG 1 cut(s) 499
BssECI CCNNGG 2 cut(s) 46, 136
BssMI GATC 3 cut(s) 314, 339, 404
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 46, 136
Bst2UI CCWGG 1 cut(s) 278
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 1 cut(s) 112
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 2 cut(s) 298, 385
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 3 cut(s) 317, 342, 407
BstMBI GATC 3 cut(s) 314, 339, 404
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 156, 260
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstUI CGCG 1 cut(s) 156
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 2 cut(s) 314, 339
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 2 cut(s) 314, 339
BsuRI GGCC 1 cut(s) 159
BtgI CCRYGG 1 cut(s) 46
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 2 cut(s) 298, 385
Cac8I GCNNGC 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 78, 158, 297
Csp6I GTAC 1 cut(s) 334
CviAII CATG 1 cut(s) 47
CviJI RGCY 7 cut(s) 51, 64, 116, 159, 281, 363, 468
CviKI_1 RGCY 7 cut(s) 51, 64, 116, 159, 281, 363, 468
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 1 cut(s) 112
DpnI GATC 3 cut(s) 316, 341, 406
DpnII GATC 3 cut(s) 314, 339, 404
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EciI GGCGGA 3 cut(s) 273, 332, 378
Eco130I CCWWGG 2 cut(s) 46, 136
Eco47I GGWCC 2 cut(s) 78, 297
Eco57I CTGAAG 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 2 cut(s) 46, 136
ErhI CCWWGG 2 cut(s) 46, 136
FaeI CATG 1 cut(s) 50
FaiI YATR 3 cut(s) 48, 206, 208
FatI CATG 1 cut(s) 46
Fnu4HI GCNGC 3 cut(s) 157, 261, 287
FokI GGATG 2 cut(s) 305, 372
Fsp4HI GCNGC 3 cut(s) 157, 261, 287
FspBI CTAG 1 cut(s) 23
GluI GCNGC 3 cut(s) 157, 261, 287
GsuI CTGGAG 1 cut(s) 482
HaeIII GGCC 1 cut(s) 159
HapII CCGG 4 cut(s) 237, 290, 450, 489
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 1 cut(s) 50
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 6 cut(s) 273, 348, 426, 453, 480, 492
HpaII CCGG 4 cut(s) 237, 290, 450, 489
HphI GGTGA 2 cut(s) 50, 152
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 5 cut(s) 7, 109, 219, 301, 458
Hpy188III TCNNGA 5 cut(s) 86, 268, 377, 395, 477
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 2 cut(s) 194, 443
HpyAV CCTTC 3 cut(s) 111, 265, 274
HpyCH4IV ACGT 2 cut(s) 42, 214
HpyCH4V TGCA 2 cut(s) 33, 309
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 260
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 2 cut(s) 42, 214
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 1 cut(s) 50
Kzo9I GATC 3 cut(s) 314, 339, 404
LmnI GCTCC 2 cut(s) 69, 286
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 42, 214
MaeIII GTNAC 2 cut(s) 38, 140
MalI GATC 3 cut(s) 316, 341, 406
MboI GATC 3 cut(s) 314, 339, 404
MboII GAAGA 4 cut(s) 96, 178, 220, 421
MflI RGATCY 2 cut(s) 314, 339
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 5 cut(s) 134, 211, 322, 402, 446
MspI CCGG 4 cut(s) 237, 290, 450, 489
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 156, 260
NcoI CCATGG 1 cut(s) 46
NdeII GATC 3 cut(s) 314, 339, 404
NlaIII CATG 1 cut(s) 50
NlaIV GGNNCC 2 cut(s) 282, 298
NmuCI GTSAC 2 cut(s) 38, 140
PfeI GAWTC 5 cut(s) 348, 426, 453, 480, 492
PflFI GACNNNGTC 1 cut(s) 437
PkrI GCNGC 3 cut(s) 158, 262, 288
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 2 cut(s) 282, 298
PspPI GGNCC 3 cut(s) 78, 158, 297
PsuI RGATCY 2 cut(s) 314, 339
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SatI GCNGC 3 cut(s) 157, 261, 287
Sau3AI GATC 3 cut(s) 314, 339, 404
Sau96I GGNCC 3 cut(s) 78, 158, 297
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 1 cut(s) 278
SetI ASST 6 cut(s) 45, 66, 217, 365, 438, 470
SfaNI GCATC 1 cut(s) 42
SinI GGWCC 2 cut(s) 78, 297
SmlI CTYRAG 3 cut(s) 268, 375, 393
SmoI CTYRAG 3 cut(s) 268, 375, 393
SpeI ACTAGT 1 cut(s) 22
SsiI CCGC 9 cut(s) 120, 150, 156, 261, 284, 287, 343, 389, 400
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 2 cut(s) 46, 136
TaiI ACGT 2 cut(s) 45, 217
TaqI TCGA 3 cut(s) 87, 432, 483
TauI GCSGC 3 cut(s) 159, 263, 289
TfiI GAWTC 5 cut(s) 348, 426, 453, 480, 492
TseFI GTSAC 2 cut(s) 38, 140
Tsp45I GTSAC 2 cut(s) 38, 140
TspDTI ATGAA 1 cut(s) 220
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 78, 297
XspI CTAG 1 cut(s) 23
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.