Rroxscaffold_6G00408920

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000803
Physical Location & Seq
Forward (+)
31440968 .. 31444457
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408920.1

Sequence Viewer

Length: 1038 bp
ATGTCGGACCCAAAACCCAGACTAGTCTGCTGTATCGGCGACGTCCACGGCTTCCACACCAAGCTCCAAAACCTCTGGTCCAATCTCGAATCCTCCATCCCCTCTTCAGATTTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGCGACCGCGGCCCAGACACCAACAAAGTCCTCGACTTCCTCATTTCCCTCCCCTCCAGATACCCACGTCAGAAACACGTCTTCATCGCCGGAAACCACGACTTCGCCTTCGCCGGCTTCTTGGGAGTCCTGCCTCCGCCGCCGGACGGGTCCGAGTTCCGCGAGGGGTGGAAGGAGTTCGAGGACAATGAGGAGAGAGAAGGGTGGTTCAAGGGCGAGGCGTACGAGGGAATGCACTTGCAGGGGAGGAGATGGGCTGGGAGTATGAAGGGGTTTAATAAGGCTAAGGGGACTGACTACCAGGGTTCCATTTATGATGCTGGGCCCACTTTTGAGTCTTATGGGGTGCCTCATGGCTCTGCCGATCTGGTGAAGGCAGTTCCTGATGATCATAAAAAGTTTCTTGCCGATATGGTTTGGGTGCATGAAGAGGATGACGTCTGTGTAGAGATTGAGGGTGAAACCAAACACTGTAAATTGATTGCTGTGCATGCCGGTTTGGAGAAAGATAAAGATGTCAAACAACAGTTGGAGTTTCTGAAAGCCAGAGATACTCGGGTGCCTAAGATAGAGGCTCTTAGTGGCAGGAAAGATGTGTGGAATATACCAGAGGTGATGGTTCTATACCTCTTTTTATCAGCAGACAAGCATGCATTTTCTTATAGCTGGTGGATTGCCAACAGCATGCGCTTGTTTTACGTCGTTGGCAATCAACCGCGTGAACTGAAAAAGACTCCAACTGTTATTGTAAGTGGACATCATGGGAAACTTCATATTGAAGGCTTAAGGCTGATTATTGATGAAGGCGGTGGTATGGCAAAGAACCCGGTGGCTGCAATTTTACTCCCCTCTATGAGGATAGTCCGTGATACAGACGTTTTGGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

345

Amino Acids

38.77

Weight (kDa)

5.97

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 9 - 116 1e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 45, 588
AccB1I GGYRCC 2 cut(s) 493, 706
AccII CGCG 3 cut(s) 156, 309, 865
AciI CCGC 8 cut(s) 120, 154, 156, 284, 287, 307, 863, 954
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 2 cut(s) 42, 585
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 2 cut(s) 293, 1002
AflII CTTAAG 1 cut(s) 931
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 2 cut(s) 358, 926
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 2 cut(s) 215, 226
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 2 cut(s) 64, 813
AluI AGCT 2 cut(s) 64, 813
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 2 cut(s) 157, 470
ApaI GGGCCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 980
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 837
AspS9I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 3 cut(s) 529, 617, 772
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 3 cut(s) 7, 78, 297
BaeGI GKGCMC 1 cut(s) 474
BaeI ACNNNNGTAYC 5 cut(s) 199, 232, 690, 723, 1008
BanI GGYRCC 2 cut(s) 493, 706
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 967
BccI CCATC 3 cut(s) 104, 393, 757
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 974
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 1036
BfrI CTTAAG 1 cut(s) 931
BisI GCNGC 3 cut(s) 157, 287, 981
BlsI GCNGC 3 cut(s) 158, 288, 982
Bme1390I CCNGG 2 cut(s) 449, 974
Bme18I GGWCC 3 cut(s) 7, 78, 297
BmeT110I CYCGRG 1 cut(s) 702
BmgBI CACGTC 2 cut(s) 215, 226
BmgT120I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
BmiI GGNNCC 6 cut(s) 9, 298, 454, 472, 495, 708
BmrFI CCNGG 2 cut(s) 449, 974
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 974
BsaHI GRCGYC 2 cut(s) 42, 585
BsaJI CCNNGG 4 cut(s) 46, 136, 154, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 1002
Bse118I RCCGGY 2 cut(s) 260, 641
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 4 cut(s) 46, 136, 154, 448
BseGI GGATG 2 cut(s) 96, 586
BseLI CCNNNNNNNGG 2 cut(s) 293, 1002
BseRI GAGGAG 2 cut(s) 353, 409
BseSI GKGCMC 1 cut(s) 474
BseXI GCAGC 1 cut(s) 967
BseYI CCCAGC 2 cut(s) 404, 467
Bsh1236I CGCG 3 cut(s) 156, 309, 865
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 159, 472
BshNI GGYRCC 2 cut(s) 493, 706
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 5 cut(s) 237, 261, 290, 642, 974
BsiWI CGTACG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 2 cut(s) 293, 1002
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 2 cut(s) 159, 472
BsoBI CYCGRG 1 cut(s) 702
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 2 cut(s) 511, 535
BspACI CCGC 8 cut(s) 120, 154, 156, 284, 287, 307, 863, 954
BspANI GGCC 2 cut(s) 159, 472
BspFNI CGCG 3 cut(s) 156, 309, 865
BspLI GGNNCC 6 cut(s) 9, 298, 454, 472, 495, 708
BspT107I GGYRCC 2 cut(s) 493, 706
BspTI CTTAAG 1 cut(s) 931
BsrFI RCCGGY 2 cut(s) 260, 641
BssAI RCCGGY 2 cut(s) 260, 641
BssECI CCNNGG 4 cut(s) 46, 136, 154, 448
BssMI GATC 2 cut(s) 511, 535
BssNI GRCGYC 2 cut(s) 42, 585
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 620, 675, 889
Bst6I CTCTTC 2 cut(s) 109, 570
BstACI GRCGYC 2 cut(s) 42, 585
BstAFI CTTAAG 1 cut(s) 931
BstC8I GCNNGC 4 cut(s) 262, 639, 798, 833
BstDEI CTNAG 3 cut(s) 432, 711, 725
BstDSI CCRYGG 2 cut(s) 46, 154
BstENI CCTNNNNNAGG 1 cut(s) 1000
BstF5I GGATG 2 cut(s) 96, 586
BstFNI CGCG 3 cut(s) 156, 309, 865
BstHHI GCGC 1 cut(s) 837
BstKTI GATC 2 cut(s) 514, 538
BstMBI GATC 2 cut(s) 511, 535
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 3 cut(s) 641, 800, 835
BstSCI CCNGG 2 cut(s) 447, 972
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 3 cut(s) 156, 309, 865
BstV1I GCAGC 1 cut(s) 967
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 2 cut(s) 159, 472
BtgI CCRYGG 2 cut(s) 46, 154
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 2 cut(s) 215, 226
BtsCI GGATG 2 cut(s) 96, 586
BtsIMutI CAGTG 1 cut(s) 616
Cac8I GCNNGC 4 cut(s) 262, 639, 798, 833
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 837
Cfr10I RCCGGY 2 cut(s) 260, 641
Cfr13I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 1 cut(s) 370
CviAII CATG 6 cut(s) 500, 572, 638, 797, 832, 908
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 3 cut(s) 432, 711, 725
DpnI GATC 2 cut(s) 513, 537
DpnII GATC 2 cut(s) 511, 535
Eam1104I CTCTTC 2 cut(s) 109, 570
EarI CTCTTC 2 cut(s) 109, 570
EciI GGCGGA 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 3 cut(s) 7, 78, 297
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 702
EcoNI CCTNNNNNAGG 1 cut(s) 1000
EcoRII CCWGG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 802
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 6 cut(s) 503, 575, 641, 800, 835, 911
FaqI GGGAC 1 cut(s) 451
FatI CATG 6 cut(s) 499, 571, 637, 796, 831, 907
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 157, 287, 981
FokI GGATG 2 cut(s) 83, 593
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 157, 287, 981
FspBI CTAG 2 cut(s) 23, 1036
GlaI GCGC 1 cut(s) 836
GluI GCNGC 3 cut(s) 157, 287, 981
GsaI CCCAGC 2 cut(s) 408, 471
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 2 cut(s) 159, 472
HapII CCGG 5 cut(s) 237, 261, 290, 642, 974
HhaI GCGC 1 cut(s) 837
Hin1I GRCGYC 2 cut(s) 42, 585
Hin1II CATG 6 cut(s) 503, 575, 641, 800, 835, 911
Hin6I GCGC 1 cut(s) 835
HinP1I GCGC 1 cut(s) 835
HinfI GANTC 4 cut(s) 89, 273, 482, 880
HpaII CCGG 5 cut(s) 237, 261, 290, 642, 974
HphI GGTGA 3 cut(s) 529, 617, 772
Hpy166II GTNNAC 3 cut(s) 46, 869, 902
Hpy188I TCNGA 5 cut(s) 7, 109, 219, 301, 687
Hpy188III TCNNGA 3 cut(s) 86, 204, 530
Hpy8I GTNNAC 3 cut(s) 46, 869, 902
Hpy99I CGWCG 2 cut(s) 44, 851
HpyAV CCTTC 7 cut(s) 265, 313, 341, 409, 514, 920, 944
HpyCH4III ACNGT 3 cut(s) 620, 675, 889
HpyCH4IV ACGT 6 cut(s) 42, 214, 225, 585, 846, 1023
HpyCH4V TGCA 6 cut(s) 382, 388, 571, 637, 800, 983
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
HpyF3I CTNAG 3 cut(s) 432, 711, 725
HpySE526I ACGT 6 cut(s) 42, 214, 225, 585, 846, 1023
Hsp92I GRCGYC 2 cut(s) 42, 585
Hsp92II CATG 6 cut(s) 503, 575, 641, 800, 835, 911
HspAI GCGC 1 cut(s) 835
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 535
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 2 cut(s) 511, 535
LmnI GCTCC 1 cut(s) 69
Lsp1109I GCAGC 1 cut(s) 967
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 23, 1036
MaeII ACGT 6 cut(s) 42, 214, 225, 585, 846, 1023
MalI GATC 2 cut(s) 513, 537
MboI GATC 2 cut(s) 511, 535
MboII GAAGA 4 cut(s) 96, 124, 220, 587
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 2 cut(s) 623, 984
MlyI GAGTC 3 cut(s) 282, 491, 874
MmeI TCCRAC 2 cut(s) 657, 908
Mph1103I ATGCAT 1 cut(s) 802
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 2 cut(s) 423, 932
MspA1I CMGCKG 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 931
MspI CCGG 5 cut(s) 237, 261, 290, 642, 974
MspR9I CCNGG 2 cut(s) 449, 974
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 3 cut(s) 156, 309, 865
MwoI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 974
NdeII GATC 2 cut(s) 511, 535
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 6 cut(s) 503, 575, 641, 800, 835, 911
NlaIV GGNNCC 6 cut(s) 9, 298, 454, 472, 495, 708
NmeAIII GCCGAG 1 cut(s) 117
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 3 cut(s) 641, 800, 835
PaeI GCATGC 3 cut(s) 641, 800, 835
PctI GAATGC 1 cut(s) 384
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 89
Pfl23II CGTACG 1 cut(s) 369
PkrI GCNGC 3 cut(s) 158, 288, 982
PleI GAGTC 3 cut(s) 281, 490, 874
PpsI GAGTC 3 cut(s) 281, 490, 874
Psp6I CCWGG 1 cut(s) 447
PspFI CCCAGC 2 cut(s) 404, 467
PspGI CCWGG 1 cut(s) 447
PspLI CGTACG 1 cut(s) 369
PspN4I GGNNCC 6 cut(s) 9, 298, 454, 472, 495, 708
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
PstNI CAGNNNCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 2 cut(s) 423, 932
SatI GCNGC 3 cut(s) 157, 287, 981
Sau3AI GATC 2 cut(s) 511, 535
Sau96I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
SchI GAGTC 3 cut(s) 282, 491, 874
ScrFI CCNGG 2 cut(s) 449, 974
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 1 cut(s) 454
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 3 cut(s) 7, 78, 297
SmlI CTYRAG 1 cut(s) 931
SmoI CTYRAG 1 cut(s) 931
SpeI ACTAGT 1 cut(s) 22
SphI GCATGC 3 cut(s) 641, 800, 835
Sse9I AATT 2 cut(s) 623, 984
SsiI CCGC 8 cut(s) 120, 154, 156, 284, 287, 307, 863, 954
SspMI CTAG 2 cut(s) 23, 1036
StyD4I CCNGG 2 cut(s) 447, 972
TaaI ACNGT 3 cut(s) 620, 675, 889
TaiI ACGT 6 cut(s) 45, 217, 228, 588, 849, 1026
TaqI TCGA 3 cut(s) 87, 180, 327
TasI AATT 2 cut(s) 623, 984
TauI GCSGC 2 cut(s) 159, 289
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 423, 932
Tru9I TTAA 2 cut(s) 423, 932
TscAI CASTG 1 cut(s) 623
TseI GCWGC 1 cut(s) 980
TspDTI ATGAA 5 cut(s) 220, 428, 588, 908, 963
TspGWI ACGGA 1 cut(s) 1001
TspRI CASTG 1 cut(s) 623
Vha464I CTTAAG 1 cut(s) 931
VpaK11BI GGWCC 3 cut(s) 7, 78, 297
XagI CCTNNNNNAGG 1 cut(s) 1000
XceI RCATGY 3 cut(s) 641, 800, 835
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 2 cut(s) 23, 1036
ZraI GACGTC 2 cut(s) 43, 586
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.