pycom12g01940

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000803
Physical Location & Seq
Reverse (-)
1620135 .. 1622271
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01940.1

Sequence Viewer

Length: 939 bp
ATGTCAGACCCAAAAGCAAAGCCAAGAATAGTGTGCTGCATCGGCGACACCCATGGCTTCCACACCAAGCTCCAAAACCTCTGGTCCAATCTCCAATCCGCCATCGACCCATCAGATTTCTCCACCGCCCTCATCATTTTCCTTGGCGATTACTGCGACCGCGGCCCGGACACCAAAAAAGTCATCGACTTTCTCGTCTCCCTGCCCTCCAGGTACCCGAATCAGAAGCACATCTTCCTGACCGGCAACCACGACCTGGCCTTCGCCGCCTTCGTGAGCGTCCTGCCGCCGCCTCCCGACGGTTCGGAGTTTTCCGAGGGGTGGAAGGAGTATGCGGATAGCGAGGATAGAGAAGGGTGGTTTAAGGGAGATGGGTACGAGAGGATGCACTTGCAGGGGAGGAGATGGGGTGGGAAGATCAAGGCTAAGTTTAATGCGGCTAAGGGGACGGAGTACAAAGGGTCAATTTATGATGCCGGGCCTACATTTGAATCCTATGGGGTCCCACATGGTTCTGCTGATTTGGTTAAGGCAGTTCCCGATGAACACAAGAAGTTTCTTGCTGATATGGTTTGGGTCCATGAAGAGGATGATGTTTGCGTAGAGACTGCGGATGGAATCAAACATTGTAGATTGATTGCTGTCCATGCTGGTTTAATGAAAGATAAAGATGTCAAACAGCAGTTGGCGTTTTTGAAAGCCAGAGACACCCGGGTGCCTAAGATAGAGGCTCTGAGTGGGAGGAAGAATGTATGGGATATACCAAGGGAACTGACAGAGAAACCAACTGTTGTCGTAAGCGGTCATCATGGGAAACTTTACATTGAAGGCCTCAGACTGATCATCGATGAAGGCGGTGGCATGGCGGAAAGACCAGTGGCTGCAGTTGTGCTCCCCTCCATGAAAATAGTCCGCGATACAGACGCATTGACAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

313

Amino Acids

34.69

Weight (kDa)

6.31

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 12 - 118 1.3e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc65I GGTACC 1 cut(s) 213
AccB1I GGYRCC 2 cut(s) 213, 715
AccB7I CCANNNNNTGG 1 cut(s) 256
AccII CGCG 2 cut(s) 162, 915
AfaI GTAC 3 cut(s) 215, 377, 455
AfiI CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
AgsI TTSAA 3 cut(s) 491, 697, 827
AjnI CCWGG 2 cut(s) 209, 255
AleI CACNNNNGTG 1 cut(s) 713
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 3 cut(s) 202, 599, 699
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 1 cut(s) 711
AoxI GGCC 4 cut(s) 163, 258, 479, 829
ApeKI GCWGC 2 cut(s) 36, 881
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp718I GGTACC 1 cut(s) 213
AspS9I GGNCC 5 cut(s) 84, 164, 479, 502, 577
AsuC2I CCSGG 4 cut(s) 167, 478, 712, 713
AvaI CYCGRG 1 cut(s) 711
AvaII GGWCC 3 cut(s) 84, 502, 577
BanI GGYRCC 2 cut(s) 213, 715
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 2 cut(s) 23, 868
BccI CCATC 5 cut(s) 110, 118, 365, 399, 608
BciT130I CCWGG 2 cut(s) 211, 257
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 4 cut(s) 167, 478, 712, 713
BcoDI GTCTC 3 cut(s) 202, 599, 699
BfaI CTAG 1 cut(s) 937
BfmI CTRYAG 1 cut(s) 882
BisI GCNGC 7 cut(s) 37, 163, 267, 287, 290, 438, 882
BlsI GCNGC 7 cut(s) 38, 164, 268, 288, 291, 439, 883
Bme1390I CCNGG 6 cut(s) 167, 211, 257, 478, 712, 713
Bme18I GGWCC 3 cut(s) 84, 502, 577
BmeT110I CYCGRG 1 cut(s) 711
BmgT120I GGNCC 5 cut(s) 84, 164, 479, 502, 577
BmiI GGNNCC 5 cut(s) 215, 503, 504, 578, 717
BmrFI CCNGG 6 cut(s) 167, 211, 257, 478, 712, 713
BmsI GCATC 3 cut(s) 48, 375, 463
BpmI CTGGAG 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 441
BpuMI CCSGG 4 cut(s) 167, 478, 712, 713
Bsa29I ATCGAT 1 cut(s) 846
BsaJI CCNNGG 6 cut(s) 52, 142, 160, 315, 711, 764
BsaXI ACNNNNNCTCC 2 cut(s) 394, 424
Bsc4I CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 875
BseBI CCWGG 2 cut(s) 211, 257
BseCI ATCGAT 1 cut(s) 846
BseDI CCNNGG 6 cut(s) 52, 142, 160, 315, 711, 764
BseGI GGATG 3 cut(s) 390, 595, 619
BseLI CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
BseMII CTCAG 2 cut(s) 725, 847
BseNI ACTGG 1 cut(s) 875
BseRI GAGGAG 1 cut(s) 415
BseXI GCAGC 2 cut(s) 23, 868
Bsh1236I CGCG 2 cut(s) 162, 915
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 4 cut(s) 165, 260, 481, 831
BshNI GGYRCC 2 cut(s) 213, 715
BshVI ATCGAT 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 711
BsiSI CCGG 4 cut(s) 167, 243, 477, 712
BslFI GGGAC 2 cut(s) 460, 488
BslI CCNNNNNNNGG 5 cut(s) 166, 256, 299, 321, 586
BsmAI GTCTC 3 cut(s) 202, 599, 699
BsmBI CGTCTC 1 cut(s) 202
BsmFI GGGAC 2 cut(s) 460, 488
BsnI GGCC 4 cut(s) 165, 260, 481, 831
BsoBI CYCGRG 1 cut(s) 711
Bsp1286I GDGCHC 1 cut(s) 894
Bsp143I GATC 2 cut(s) 417, 840
Bsp19I CCATGG 1 cut(s) 52
BspANI GGCC 4 cut(s) 165, 260, 481, 831
BspCNI CTCAG 2 cut(s) 726, 846
BspDI ATCGAT 1 cut(s) 846
BspFNI CGCG 2 cut(s) 162, 915
BspLI GGNNCC 5 cut(s) 215, 503, 504, 578, 717
BspMAI CTGCAG 1 cut(s) 886
BspT107I GGYRCC 2 cut(s) 213, 715
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 1 cut(s) 875
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 6 cut(s) 52, 142, 160, 315, 711, 764
BssMI GATC 2 cut(s) 417, 840
BssT1I CCWWGG 3 cut(s) 52, 142, 764
Bst2UI CCWGG 2 cut(s) 211, 257
Bst4CI ACNGT 2 cut(s) 302, 790
Bst6I CTCTTC 1 cut(s) 579
BstDEI CTNAG 5 cut(s) 426, 441, 720, 734, 833
BstDSI CCRYGG 2 cut(s) 52, 160
BstF5I GGATG 3 cut(s) 390, 595, 619
BstFNI CGCG 2 cut(s) 162, 915
BstKTI GATC 2 cut(s) 420, 843
BstMAI GTCTC 3 cut(s) 202, 599, 699
BstMBI GATC 2 cut(s) 417, 840
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 5 cut(s) 42, 153, 162, 266, 647
BstNI CCWGG 2 cut(s) 211, 257
BstSCI CCNGG 6 cut(s) 165, 209, 255, 476, 710, 711
BstSFI CTRYAG 1 cut(s) 882
BstUI CGCG 2 cut(s) 162, 915
BstV1I GCAGC 2 cut(s) 23, 868
Bsu15I ATCGAT 1 cut(s) 846
BsuRI GGCC 4 cut(s) 165, 260, 481, 831
BsuTUI ATCGAT 1 cut(s) 846
BtgI CCRYGG 2 cut(s) 52, 160
BtsCI GGATG 3 cut(s) 390, 595, 619
BtsIMutI CAGTG 1 cut(s) 882
CaiI CAGNNNCTG 1 cut(s) 881
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 5 cut(s) 84, 164, 479, 502, 577
Cfr42I CCGCGG 1 cut(s) 163
Cfr9I CCCGGG 1 cut(s) 711
ClaI ATCGAT 1 cut(s) 846
CseI GACGC 2 cut(s) 268, 932
Csp6I GTAC 3 cut(s) 214, 376, 454
CviAII CATG 7 cut(s) 53, 509, 581, 647, 809, 862, 901
CviQI GTAC 3 cut(s) 214, 376, 454
DdeI CTNAG 5 cut(s) 426, 441, 720, 734, 833
DpnI GATC 2 cut(s) 419, 842
DpnII GATC 2 cut(s) 417, 840
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
EciI GGCGGA 2 cut(s) 88, 881
Eco130I CCWWGG 3 cut(s) 52, 142, 764
Eco147I AGGCCT 1 cut(s) 831
Eco47I GGWCC 3 cut(s) 84, 502, 577
Eco88I CYCGRG 1 cut(s) 711
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 209, 255
EcoT14I CCWWGG 3 cut(s) 52, 142, 764
ErhI CCWWGG 3 cut(s) 52, 142, 764
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 7 cut(s) 56, 512, 584, 650, 812, 865, 904
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 2 cut(s) 460, 488
FatI CATG 7 cut(s) 52, 508, 580, 646, 808, 861, 900
FbaI TGATCA 1 cut(s) 840
Fnu4HI GCNGC 7 cut(s) 37, 163, 267, 287, 290, 438, 882
FokI GGATG 3 cut(s) 397, 602, 626
Fsp4HI GCNGC 7 cut(s) 37, 163, 267, 287, 290, 438, 882
FspBI CTAG 1 cut(s) 937
GluI GCNGC 7 cut(s) 37, 163, 267, 287, 290, 438, 882
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 4 cut(s) 165, 260, 481, 831
HapII CCGG 4 cut(s) 167, 243, 477, 712
HgaI GACGC 2 cut(s) 268, 932
Hin1II CATG 7 cut(s) 56, 512, 584, 650, 812, 865, 904
HinfI GANTC 3 cut(s) 220, 491, 618
HpaII CCGG 4 cut(s) 167, 243, 477, 712
Hpy188I TCNGA 7 cut(s) 7, 115, 225, 307, 316, 735, 836
Hpy188III TCNNGA 4 cut(s) 238, 274, 296, 539
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 6 cut(s) 271, 280, 319, 347, 821, 845
HpyCH4III ACNGT 2 cut(s) 302, 790
HpyCH4V TGCA 4 cut(s) 39, 388, 394, 884
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 153, 162, 266, 647
HpyF3I CTNAG 5 cut(s) 426, 441, 720, 734, 833
Hsp92II CATG 7 cut(s) 56, 512, 584, 650, 812, 865, 904
KflI GGGWCCC 1 cut(s) 502
KpnI GGTACC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 840
KspI CCGCGG 1 cut(s) 163
Kzo9I GATC 2 cut(s) 417, 840
LmnI GCTCC 2 cut(s) 75, 897
Lsp1109I GCAGC 2 cut(s) 23, 868
LweI GCATC 3 cut(s) 48, 375, 463
MaeI CTAG 1 cut(s) 937
MalI GATC 2 cut(s) 419, 842
MboI GATC 2 cut(s) 417, 840
MboII GAAGA 4 cut(s) 226, 427, 596, 757
MhlI GDGCHC 1 cut(s) 894
MluCI AATT 1 cut(s) 465
MseI TTAA 4 cut(s) 363, 432, 528, 656
MslI CAYNNNNRTG 1 cut(s) 713
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 4 cut(s) 167, 243, 477, 712
MspR9I CCNGG 6 cut(s) 167, 211, 257, 478, 712, 713
MvaI CCWGG 2 cut(s) 211, 257
MvnI CGCG 2 cut(s) 162, 915
MwoI GCNNNNNNNGC 5 cut(s) 42, 153, 162, 266, 647
NciI CCSGG 4 cut(s) 167, 478, 712, 713
NcoI CCATGG 1 cut(s) 52
NdeII GATC 2 cut(s) 417, 840
NlaIII CATG 7 cut(s) 56, 512, 584, 650, 812, 865, 904
NlaIV GGNNCC 5 cut(s) 215, 503, 504, 578, 717
OliI CACNNNNGTG 1 cut(s) 713
PceI AGGCCT 1 cut(s) 831
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 270
PfeI GAWTC 3 cut(s) 220, 491, 618
PflMI CCANNNNNTGG 1 cut(s) 256
PkrI GCNGC 7 cut(s) 38, 164, 268, 288, 291, 439, 883
PpuMI RGGWCCY 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 209, 255
PspGI CCWGG 2 cut(s) 209, 255
PspN4I GGNNCC 5 cut(s) 215, 503, 504, 578, 717
PspPI GGNCC 5 cut(s) 84, 164, 479, 502, 577
PspPPI RGGWCCY 1 cut(s) 502
PstI CTGCAG 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 881
RsaI GTAC 3 cut(s) 215, 377, 455
RsaNI GTAC 3 cut(s) 214, 376, 454
RseI CAYNNNNRTG 1 cut(s) 713
SacII CCGCGG 1 cut(s) 163
SaqAI TTAA 4 cut(s) 363, 432, 528, 656
SatI GCNGC 7 cut(s) 37, 163, 267, 287, 290, 438, 882
Sau3AI GATC 2 cut(s) 417, 840
Sau96I GGNCC 5 cut(s) 84, 164, 479, 502, 577
ScrFI CCNGG 6 cut(s) 167, 211, 257, 478, 712, 713
SduI GDGCHC 1 cut(s) 894
SetI ASST 4 cut(s) 72, 81, 215, 258
SfaNI GCATC 3 cut(s) 48, 375, 463
SfcI CTRYAG 1 cut(s) 882
Sfr303I CCGCGG 1 cut(s) 163
SgrBI CCGCGG 1 cut(s) 163
SinI GGWCC 3 cut(s) 84, 502, 577
SmaI CCCGGG 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 713
Sse9I AATT 1 cut(s) 465
SseBI AGGCCT 1 cut(s) 831
SspMI CTAG 1 cut(s) 937
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 6 cut(s) 165, 209, 255, 476, 710, 711
StyI CCWWGG 3 cut(s) 52, 142, 764
TaaI ACNGT 2 cut(s) 302, 790
TaqI TCGA 3 cut(s) 105, 186, 846
TasI AATT 1 cut(s) 465
TatI WGTACW 1 cut(s) 453
TauI GCSGC 5 cut(s) 165, 269, 289, 292, 440
TfiI GAWTC 3 cut(s) 220, 491, 618
Tru1I TTAA 4 cut(s) 363, 432, 528, 656
Tru9I TTAA 4 cut(s) 363, 432, 528, 656
TscAI CASTG 1 cut(s) 882
TseI GCWGC 2 cut(s) 36, 881
TspDTI ATGAA 5 cut(s) 558, 597, 674, 864, 917
TspGWI ACGGA 1 cut(s) 464
TspMI CCCGGG 1 cut(s) 711
TspRI CASTG 1 cut(s) 882
Van91I CCANNNNNTGG 1 cut(s) 256
VpaK11BI GGWCC 3 cut(s) 84, 502, 577
XmaI CCCGGG 1 cut(s) 711
XspI CTAG 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.