Rmu_sc0000058.1_g000003

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000058.1
OG0000803
Physical Location & Seq
Forward (+)
18136 .. 19782
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000058.1_g000003.1.cds

Sequence Viewer

Length: 453 bp
atgccgaacctaccgaaaccgaccggaaaccggctgaaccgccgccttcggcgccgacaaatacggttgccagtcgatcgcggttgtaaaaaaaggcaaaccgatgagtttcggttcggtaacggtgatggccaaaaaatgtcggttaccgacttcgccacctccctggtcatcttcctcgacgactactgcggccgcggttcagacaccaacgaagtccttgacttcttcgtcgctctcccctccagatacccacatcagaaagacgtcttcattgccggaaaccatgacttcgccttcgccgccttgttgagagtcctgcctctgccaccggacgggtccgagttccgtgaggggtggaaggagttcgaggacaaggaggagagagaaaaagatttagagacaaagagagggcaagaagatgtaaaaagaaaaagagagaaaagaagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

150

Amino Acids

17.61

Weight (kDa)

9.51

Isoelectric Point (pI)

56.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 51
AccII CGCG 2 cut(s) 81, 198
AciI CCGC 7 cut(s) 40, 43, 81, 192, 196, 198, 303
AcoI YGGCCR 2 cut(s) 130, 193
AcyI GRCGYC 2 cut(s) 52, 267
AfiI CCNNNNNNNGG 2 cut(s) 30, 335
AjnI CCWGG 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 395
AoxI GGCC 2 cut(s) 130, 193
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 339
BalI TGGCCA 1 cut(s) 132
BanI GGYRCC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 262
BccI CCATC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 395
BfoI RGCGCY 1 cut(s) 55
BisI GCNGC 4 cut(s) 43, 193, 196, 303
BlsI GCNGC 4 cut(s) 44, 194, 197, 304
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 2 cut(s) 53, 340
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 262
BpmI CTGGAG 1 cut(s) 229
BsaHI GRCGYC 2 cut(s) 52, 267
BsaJI CCNNGG 2 cut(s) 165, 196
BsaWI WCCGGW 2 cut(s) 23, 331
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 335
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 165, 196
BseLI CCNNNNNNNGG 2 cut(s) 30, 335
BseMI GCAATG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 1 cut(s) 395
BseX3I CGGCCG 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 81, 198
Bsh1285I CGRYCG 3 cut(s) 24, 79, 196
BshFI GGCC 2 cut(s) 132, 195
BshNI GGYRCC 1 cut(s) 51
BsiEI CGRYCG 3 cut(s) 24, 79, 196
BsiSI CCGG 4 cut(s) 24, 31, 279, 332
BslI CCNNNNNNNGG 2 cut(s) 30, 335
BsmAI GTCTC 1 cut(s) 395
BsnI GGCC 2 cut(s) 132, 195
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 7 cut(s) 40, 43, 81, 192, 196, 198, 303
BspANI GGCC 2 cut(s) 132, 195
BspFNI CGCG 2 cut(s) 81, 198
BspLI GGNNCC 2 cut(s) 53, 340
BspT107I GGYRCC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 2 cut(s) 165, 196
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 2 cut(s) 52, 267
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 66, 125
BstACI GRCGYC 2 cut(s) 52, 267
BstDSI CCRYGG 1 cut(s) 196
BstEII GGTNACC 1 cut(s) 145
BstFNI CGCG 2 cut(s) 81, 198
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 76
BstMCI CGRYCG 3 cut(s) 24, 79, 196
BstMWI GCNNNNNNNGC 2 cut(s) 51, 302
BstNI CCWGG 1 cut(s) 167
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 165
BstUI CGCG 2 cut(s) 81, 198
BstV2I GAAGAC 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 166
BstZI CGGCCG 1 cut(s) 193
BsuRI GGCC 2 cut(s) 132, 195
BtgI CCRYGG 1 cut(s) 196
CciNI GCGGCCGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 339
Cfr42I CCGCGG 1 cut(s) 199
CviAII CATG 1 cut(s) 287
CviJI RGCY 3 cut(s) 34, 132, 195
CviKI_1 RGCY 3 cut(s) 34, 132, 195
DinI GGCGCC 1 cut(s) 53
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 2 cut(s) 130, 193
EagI CGGCCG 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 165
EgeI GGCGCC 1 cut(s) 53
EheI GGCGCC 1 cut(s) 53
FaeI CATG 1 cut(s) 290
FaiI YATR 1 cut(s) 288
FatI CATG 1 cut(s) 286
Fnu4HI GCNGC 4 cut(s) 43, 193, 196, 303
Fsp4HI GCNGC 4 cut(s) 43, 193, 196, 303
GlaI GCGC 1 cut(s) 53
GluI GCNGC 4 cut(s) 43, 193, 196, 303
GsuI CTGGAG 1 cut(s) 229
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 2 cut(s) 132, 195
HapII CCGG 4 cut(s) 24, 31, 279, 332
HhaI GCGC 1 cut(s) 54
Hin1I GRCGYC 2 cut(s) 52, 267
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HinfI GANTC 1 cut(s) 315
HpaII CCGG 4 cut(s) 24, 31, 279, 332
HphI GGTGA 1 cut(s) 137
Hpy188I TCNGA 3 cut(s) 205, 261, 343
Hpy188III TCNNGA 1 cut(s) 246
Hpy99I CGWCG 2 cut(s) 185, 236
HpyAV CCTTC 3 cut(s) 56, 307, 355
HpyCH4III ACNGT 2 cut(s) 66, 125
HpyCH4IV ACGT 1 cut(s) 267
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 302
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 2 cut(s) 52, 267
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 1 cut(s) 52
KasI GGCGCC 1 cut(s) 51
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 1 cut(s) 76
LpnPI CCDG 9 cut(s) 37, 44, 84, 152, 179, 259, 292, 332, 345
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 119, 145
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 4 cut(s) 166, 220, 262, 431
MlsI TGGCCA 1 cut(s) 132
MluNI TGGCCA 1 cut(s) 132
Mly113I GGCGCC 1 cut(s) 52
MlyI GAGTC 1 cut(s) 324
MnlI CCTC 8 cut(s) 172, 188, 253, 333, 346, 364, 373, 404
Mox20I TGGCCA 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 365
MscI TGGCCA 1 cut(s) 132
Msp20I TGGCCA 1 cut(s) 132
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 4 cut(s) 24, 31, 279, 332
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 2 cut(s) 81, 198
MwoI GCNNNNNNNGC 2 cut(s) 51, 302
NarI GGCGCC 1 cut(s) 52
NdeII GATC 1 cut(s) 76
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 2 cut(s) 53, 340
NotI GCGGCCGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 365
PkrI GCNGC 4 cut(s) 44, 194, 197, 304
Ple19I CGATCG 1 cut(s) 79
PleI GAGTC 1 cut(s) 323
PluTI GGCGCC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 165
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 53, 340
PspPI GGNCC 1 cut(s) 339
PvuI CGATCG 1 cut(s) 79
SacII CCGCGG 1 cut(s) 199
SatI GCNGC 4 cut(s) 43, 193, 196, 303
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 1 cut(s) 339
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 1 cut(s) 167
SetI ASST 3 cut(s) 12, 164, 270
SfoI GGCGCC 1 cut(s) 53
Sfr303I CCGCGG 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 199
SinI GGWCC 1 cut(s) 339
SsiI CCGC 7 cut(s) 40, 43, 81, 192, 196, 198, 303
SspDI GGCGCC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 66, 125
TaiI ACGT 1 cut(s) 270
TaqI TCGA 3 cut(s) 75, 180, 369
TauI GCSGC 4 cut(s) 45, 195, 198, 305
TspDTI ATGAA 1 cut(s) 262
TspGWI ACGGA 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 365
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.