Rorug03G0125000

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000803
Physical Location & Seq
Forward (+)
10077438 .. 10081175
3738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0125000.1

Sequence Viewer

Length: 1278 bp
ATGGAGCAGTCTCTGCTTTCAGAGCAACGAAATGGATCAGAGGAGAAGTCGAGTGAGAACTGGAGCTCGTACCAGTACGTGGGGAGATCCGGCTCTGTGATTCCGACGGCTTCGTTGGCCGGAACTGAAGTCAGCGTCGAAGAGATACGATCAGCGGCGGCTTCTGAGCACTACCCACCTTCTCTTCATAGCGGTTTGGTTAGTTCGCCGGAGCCTGATCTCGATGAGCAATCTGTTGTTTACCAAGGTGGATATGGAGGAGGTGCTGGCAGCACCACAAGTGAGTTTCATAGGCAAACACTGGATGAAGTAGAGATACGAGAGTTGCTCATTGATCATGTTGGCCATAGATGCTGTTGGGGAAGTCGTCCTGCTCGCACCTGGAAAATTGAAAGAGTGGAAGATTGCAACGTTTATGTGGGAACACTAGAGACCTTCATTGAAGAAAGGGAAACTGTAAGAGAAACAGAGCCATACCTTGTTGGCAGTATTGATGGAAGGGATAAGGGACCCGAACTGGGAGTCTGGGAATTGGATTTGAAGTCCCAATTTCCTGTTTTATTTATACCTCATAAAGAATCACGGGAGATAATTCCTCATTCAGAGTCCGTTGCGAAATGCTCAGGTTGTGCAGGGCGAGGGGGTACCTTCTGTCCTAAATGCAATGCAAATCAAGAACCTGGATTCTACAAAGAGAACCAGATGGCTCAGTGCTCTGCTTGTCATGGAAGGGGCTTACTTGCTCATAAAGATGGATCTGACTCAATATGTGTGACTTGCAATGGCAAGGGACAGATTCCTTGTGCAACATGTGGATCTCGTGGTCTAGTTAAATGTTTGACATGTGAAGGAAGCGGTTCTCTTCGGTCCCGCAATGTTGCCCTTGTTAGATGGAAGACACTTTTAACTAGAAAGGTAAGTGCAACAAGCGGAGCAGCATCTGTTCCCGATGATGTTTTCCACAGAGCCAAAGGGGTCCAGTTGTGCAACACCCAGGCGCATCAATGCACTCCAGCCTTCTTTGCCGACTCTTATTTCCTCAACAAGTTCTCATCGGAAGTCATCGCTGACAGAGCTCCTGTGCCCCTCTCTGCAAGGGTCATAAGCGAAAGGCACACCATATCCGTAGTTCCAGTGACACGTGTCACAATGGCTCATCGCAACCGATTATTCAGTTTCTACATCATTGGGTTTAGCAGGGAGGTGTATCTAAAGGACTACTATCCCTCTCGGTTTTGCTGGGGCTTGTGCCCGTGCTTGGAGTGGTTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

425

Amino Acids

46.88

Weight (kDa)

6.37

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 644
AccB7I CCANNNNNTGG 1 cut(s) 79
AciI CCGC 6 cut(s) 155, 158, 192, 855, 871, 930
AclI AACGTT 1 cut(s) 411
AclWI GGATC 4 cut(s) 43, 81, 763, 823
AcoI YGGCCR 2 cut(s) 117, 343
AcuI CTGAAG 1 cut(s) 147
AcvI CACGTG 1 cut(s) 1142
AfaI GTAC 3 cut(s) 71, 77, 646
AfiI CCNNNNNNNGG 3 cut(s) 79, 518, 1258
AflIII ACRYGT 4 cut(s) 809, 842, 1139, 1141
AgsI TTSAA 4 cut(s) 392, 443, 541, 1270
AjnI CCWGG 3 cut(s) 380, 679, 993
AluBI AGCT 2 cut(s) 66, 1076
AluI AGCT 2 cut(s) 66, 1076
Alw21I GWGCWC 4 cut(s) 68, 171, 716, 1078
Alw26I GTCTC 2 cut(s) 15, 425
AlwI GGATC 4 cut(s) 43, 81, 763, 823
AlwNI CAGNNNCTG 2 cut(s) 13, 941
AoxI GGCC 2 cut(s) 117, 343
ApeKI GCWGC 2 cut(s) 270, 935
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
Asp700I GAANNNNTTC 1 cut(s) 856
Asp718I GGTACC 1 cut(s) 644
AspLEI GCGC 1 cut(s) 1000
AspS9I GGNCC 3 cut(s) 509, 867, 976
AvaII GGWCC 3 cut(s) 509, 867, 976
BaeGI GKGCMC 2 cut(s) 1086, 1253
BaeI ACNNNNGTAYC 2 cut(s) 308, 341
BalI TGGCCA 1 cut(s) 345
BanI GGYRCC 1 cut(s) 644
BanII GRGCYC 2 cut(s) 68, 1078
BarI GAAGNNNNNNTAC 2 cut(s) 300, 332
BauI CACGAG 1 cut(s) 819
BbrPI CACGTG 1 cut(s) 1142
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 4 cut(s) 68, 171, 716, 1078
BbvI GCAGC 2 cut(s) 282, 947
BccI CCATC 4 cut(s) 488, 697, 746, 885
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 3 cut(s) 382, 681, 995
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 15, 425
BfaI CTAG 3 cut(s) 428, 827, 909
BisI GCNGC 4 cut(s) 156, 159, 271, 936
BlsI GCNGC 4 cut(s) 157, 160, 272, 937
Bme1390I CCNGG 3 cut(s) 382, 681, 995
Bme18I GGWCC 3 cut(s) 509, 867, 976
BmgT120I GGNCC 3 cut(s) 509, 867, 976
BmiI GGNNCC 6 cut(s) 213, 510, 511, 646, 869, 977
BmrFI CCNGG 3 cut(s) 382, 681, 995
BmrI ACTGGG 1 cut(s) 527
BmsI GCATC 3 cut(s) 341, 947, 1009
BmuI ACTGGG 1 cut(s) 527
BoxI GACNNNNGTC 1 cut(s) 1142
BpiI GAAGAC 1 cut(s) 902
BplI GAGNNNNNCTC 2 cut(s) 312, 344
BpmI CTGGAG 2 cut(s) 82, 996
Bpu10I CCTNAGC 1 cut(s) 622
BsaAI YACGTR 2 cut(s) 79, 1142
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 2 cut(s) 244, 993
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 518, 1258
Bse1I ACTGG 6 cut(s) 65, 73, 306, 522, 979, 1133
Bse3DI GCAATG 3 cut(s) 670, 787, 880
BseBI CCWGG 3 cut(s) 382, 681, 995
BseDI CCNNGG 2 cut(s) 244, 993
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 3 cut(s) 79, 518, 1258
BseMI GCAATG 3 cut(s) 670, 787, 880
BseMII CTCAG 3 cut(s) 156, 636, 722
BseNI ACTGG 6 cut(s) 65, 73, 306, 522, 979, 1133
BseRI GAGGAG 2 cut(s) 56, 273
BseSI GKGCMC 2 cut(s) 1086, 1253
BseXI GCAGC 2 cut(s) 282, 947
BseYI CCCAGC 1 cut(s) 1239
BsgI GTGCAG 1 cut(s) 651
BshFI GGCC 2 cut(s) 119, 345
BshNI GGYRCC 1 cut(s) 644
BsiHKAI GWGCWC 4 cut(s) 68, 171, 716, 1078
BsiSI CCGG 3 cut(s) 90, 120, 209
BslFI GGGAC 4 cut(s) 522, 529, 804, 853
BslI CCNNNNNNNGG 3 cut(s) 79, 518, 1258
BsmAI GTCTC 2 cut(s) 15, 425
BsmFI GGGAC 4 cut(s) 522, 529, 804, 853
BsnI GGCC 2 cut(s) 119, 345
Bso31I GGTCTC 1 cut(s) 425
Bsp1286I GDGCHC 6 cut(s) 68, 171, 716, 1078, 1086, 1253
Bsp143I GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
BspACI CCGC 6 cut(s) 155, 158, 192, 855, 871, 930
BspANI GGCC 2 cut(s) 119, 345
BspCNI CTCAG 3 cut(s) 157, 635, 721
BspLI GGNNCC 6 cut(s) 213, 510, 511, 646, 869, 977
BspPI GGATC 4 cut(s) 43, 81, 763, 823
BspT107I GGYRCC 1 cut(s) 644
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 3 cut(s) 670, 787, 880
BsrI ACTGG 6 cut(s) 65, 73, 306, 522, 979, 1133
BssECI CCNNGG 2 cut(s) 244, 993
BssMI GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
BssSI CACGAG 1 cut(s) 819
BssT1I CCWWGG 1 cut(s) 244
Bst2BI CACGAG 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 382, 681, 995
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 3 cut(s) 135, 189, 867
BstAPI GCANNNNNTGC 1 cut(s) 13
BstBAI YACGTR 2 cut(s) 79, 1142
BstC8I GCNNGC 2 cut(s) 268, 376
BstDEI CTNAG 3 cut(s) 165, 622, 708
BstF5I GGATG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 1000
BstKTI GATC 7 cut(s) 38, 89, 152, 220, 337, 758, 818
BstMAI GTCTC 2 cut(s) 15, 425
BstMBI GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
BstMWI GCNNNNNNNGC 6 cut(s) 13, 22, 116, 351, 1022, 1073
BstNI CCWGG 3 cut(s) 382, 681, 995
BstNSI RCATGY 2 cut(s) 813, 846
BstPAI GACNNNNGTC 1 cut(s) 1142
BstSCI CCNGG 3 cut(s) 380, 679, 993
BstSLI GKGCMC 2 cut(s) 1086, 1253
BstV1I GCAGC 2 cut(s) 282, 947
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 3 cut(s) 86, 755, 815
BstYI RGATCY 3 cut(s) 86, 755, 815
BsuRI GGCC 2 cut(s) 119, 345
BtgZI GCGATG 2 cut(s) 1048, 1142
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 3 cut(s) 299, 716, 1140
Cac8I GCNNGC 2 cut(s) 268, 376
CaiI CAGNNNCTG 2 cut(s) 13, 941
CfoI GCGC 1 cut(s) 1000
Cfr13I GGNCC 3 cut(s) 509, 867, 976
CseI GACGC 1 cut(s) 124
Csp6I GTAC 3 cut(s) 70, 76, 645
CviAII CATG 4 cut(s) 338, 725, 810, 843
CviQI GTAC 3 cut(s) 70, 76, 645
DdeI CTNAG 3 cut(s) 165, 622, 708
DpnI GATC 7 cut(s) 37, 88, 151, 219, 336, 757, 817
DpnII GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
EaeI YGGCCR 2 cut(s) 117, 343
Eam1104I CTCTTC 3 cut(s) 135, 189, 867
EarI CTCTTC 3 cut(s) 135, 189, 867
Ecl136II GAGCTC 2 cut(s) 66, 1076
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 2 cut(s) 68, 1078
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 3 cut(s) 509, 867, 976
Eco53kI GAGCTC 2 cut(s) 66, 1076
Eco57I CTGAAG 1 cut(s) 147
Eco72I CACGTG 1 cut(s) 1142
EcoICRI GAGCTC 2 cut(s) 66, 1076
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 380, 679, 993
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 2 cut(s) 68, 1078
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 4 cut(s) 341, 728, 813, 846
FaqI GGGAC 4 cut(s) 522, 529, 804, 853
FatI CATG 4 cut(s) 337, 724, 809, 842
FauI CCCGC 1 cut(s) 878
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 4 cut(s) 156, 159, 271, 936
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 2 cut(s) 68, 1078
Fsp4HI GCNGC 4 cut(s) 156, 159, 271, 936
FspBI CTAG 3 cut(s) 428, 827, 909
GlaI GCGC 1 cut(s) 999
GluI GCNGC 4 cut(s) 156, 159, 271, 936
GsaI CCCAGC 1 cut(s) 1243
GsuI CTGGAG 2 cut(s) 82, 996
HaeIII GGCC 2 cut(s) 119, 345
HapII CCGG 3 cut(s) 90, 120, 209
HgaI GACGC 1 cut(s) 124
HhaI GCGC 1 cut(s) 1000
Hin1II CATG 4 cut(s) 341, 728, 813, 846
Hin6I GCGC 1 cut(s) 998
HinP1I GCGC 1 cut(s) 998
HinfI GANTC 8 cut(s) 100, 522, 578, 605, 684, 761, 796, 1028
HpaII CCGG 3 cut(s) 90, 120, 209
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 7 cut(s) 22, 40, 105, 166, 604, 760, 1057
Hpy188III TCNNGA 3 cut(s) 221, 674, 947
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 2 cut(s) 109, 140
HpyAV CCTTC 7 cut(s) 189, 445, 492, 658, 723, 842, 1027
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 3 cut(s) 78, 411, 1141
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 22, 116, 351, 1022, 1073
HpyF3I CTNAG 3 cut(s) 165, 622, 708
HpySE526I ACGT 3 cut(s) 78, 411, 1141
Hsp92II CATG 4 cut(s) 341, 728, 813, 846
HspAI GCGC 1 cut(s) 998
KflI GGGWCCC 1 cut(s) 509
KpnI GGTACC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
LmnI GCTCC 5 cut(s) 4, 63, 211, 932, 1081
Lsp1109I GCAGC 2 cut(s) 282, 947
LweI GCATC 3 cut(s) 341, 947, 1009
MaeI CTAG 3 cut(s) 428, 827, 909
MaeII ACGT 3 cut(s) 78, 411, 1141
MaeIII GTNAC 3 cut(s) 772, 1135, 1144
MalI GATC 7 cut(s) 37, 88, 151, 219, 336, 757, 817
MboI GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
MboII GAAGA 6 cut(s) 152, 176, 413, 455, 854, 907
MflI RGATCY 3 cut(s) 86, 755, 815
MhlI GDGCHC 6 cut(s) 68, 171, 716, 1078, 1086, 1253
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 4 cut(s) 387, 530, 548, 591
MluNI TGGCCA 1 cut(s) 345
MlyI GAGTC 4 cut(s) 531, 614, 755, 1022
MmeI TCCRAC 1 cut(s) 128
Mox20I TGGCCA 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 856
MscI TGGCCA 1 cut(s) 345
MseI TTAA 2 cut(s) 831, 905
MslI CAYNNNNRTG 1 cut(s) 750
Msp20I TGGCCA 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 90, 120, 209
MspR9I CCNGG 3 cut(s) 382, 681, 995
MvaI CCWGG 3 cut(s) 382, 681, 995
MwoI GCNNNNNNNGC 6 cut(s) 13, 22, 116, 351, 1022, 1073
NdeII GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
NlaIII CATG 4 cut(s) 341, 728, 813, 846
NlaIV GGNNCC 6 cut(s) 213, 510, 511, 646, 869, 977
NmuCI GTSAC 3 cut(s) 772, 1135, 1144
NspI RCATGY 2 cut(s) 813, 846
PciI ACATGT 2 cut(s) 809, 842
PdmI GAANNNNTTC 1 cut(s) 856
PfeI GAWTC 4 cut(s) 100, 578, 684, 796
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 4 cut(s) 157, 160, 272, 937
PleI GAGTC 4 cut(s) 530, 613, 755, 1022
PmaCI CACGTG 1 cut(s) 1142
PmlI CACGTG 1 cut(s) 1142
PpsI GAGTC 4 cut(s) 530, 613, 755, 1022
Ppu21I YACGTR 2 cut(s) 79, 1142
PpuMI RGGWCCY 1 cut(s) 509
PscI ACATGT 2 cut(s) 809, 842
PshAI GACNNNNGTC 1 cut(s) 1142
Psp124BI GAGCTC 2 cut(s) 68, 1078
Psp1406I AACGTT 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 3 cut(s) 380, 679, 993
PspCI CACGTG 1 cut(s) 1142
PspFI CCCAGC 1 cut(s) 1239
PspGI CCWGG 3 cut(s) 380, 679, 993
PspN4I GGNNCC 6 cut(s) 213, 510, 511, 646, 869, 977
PspPI GGNCC 3 cut(s) 509, 867, 976
PspPPI RGGWCCY 1 cut(s) 509
PstNI CAGNNNCTG 2 cut(s) 13, 941
PsuI RGATCY 3 cut(s) 86, 755, 815
RsaI GTAC 3 cut(s) 71, 77, 646
RsaNI GTAC 3 cut(s) 70, 76, 645
RseI CAYNNNNRTG 1 cut(s) 750
SacI GAGCTC 2 cut(s) 68, 1078
SaqAI TTAA 2 cut(s) 831, 905
SatI GCNGC 4 cut(s) 156, 159, 271, 936
Sau3AI GATC 7 cut(s) 35, 86, 149, 217, 334, 755, 815
Sau96I GGNCC 3 cut(s) 509, 867, 976
SchI GAGTC 4 cut(s) 531, 614, 755, 1022
ScrFI CCNGG 3 cut(s) 382, 681, 995
SduI GDGCHC 6 cut(s) 68, 171, 716, 1078, 1086, 1253
SfaNI GCATC 3 cut(s) 341, 947, 1009
SinI GGWCC 3 cut(s) 509, 867, 976
SmiMI CAYNNNNRTG 1 cut(s) 750
Sse9I AATT 4 cut(s) 387, 530, 548, 591
SsiI CCGC 6 cut(s) 155, 158, 192, 855, 871, 930
SspMI CTAG 3 cut(s) 428, 827, 909
SstI GAGCTC 2 cut(s) 68, 1078
StyD4I CCNGG 3 cut(s) 380, 679, 993
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 3 cut(s) 81, 414, 1144
TaqI TCGA 3 cut(s) 50, 138, 222
TaqII GACCGA 1 cut(s) 855
TasI AATT 4 cut(s) 387, 530, 548, 591
TauI GCSGC 2 cut(s) 158, 161
TfiI GAWTC 4 cut(s) 100, 578, 684, 796
Tru1I TTAA 2 cut(s) 831, 905
Tru9I TTAA 2 cut(s) 831, 905
TscAI CASTG 3 cut(s) 306, 716, 1140
TseFI GTSAC 3 cut(s) 772, 1135, 1144
TseI GCWGC 2 cut(s) 270, 935
Tsp45I GTSAC 3 cut(s) 772, 1135, 1144
TspDTI ATGAA 4 cut(s) 176, 278, 321, 427
TspGWI ACGGA 2 cut(s) 598, 1114
TspRI CASTG 3 cut(s) 306, 716, 1140
Van91I CCANNNNNTGG 1 cut(s) 79
VpaK11BI GGWCC 3 cut(s) 509, 867, 976
XceI RCATGY 2 cut(s) 813, 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 856
XspI CTAG 3 cut(s) 428, 827, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.