Prupe.3G199100_v2.0.a1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000803
Physical Location & Seq
Forward (+)
20849026 .. 20849740
715 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G199100.1

Sequence Viewer

Length: 435 bp
ATGCCCATCTCAAAAGATCCCACCAAGAAAGGAAACCTCAGGATCAAGTTTGACGTCGTCTTTCCATCGAAGCAGCAGAACCATGAGGAACCCCATAAGACGCAAAGGTGGGCTCAGCATCATAAATCGACCCTTCAGATTTCAACACTACCCTCCAAGTACCCAAATCAGAGACACGTGTTTCTGTCTGGGAACCATGACCTGGCCTTTGCAGCCTTTGTGGGGGTCCTGCCTAAGCCTTTGGATGGCTCTGAGTTTTCTGTGGCTTGGAAGGAGTATGAGGCCAATGAAGAGAGAGAAGGGTGGTTTAAGGGTGATGGGTATGAGAGAATTGTTTTTATTTTTATTTATTTTTTATTTTTTATTTTTTTGGGAACTACAAGTCATGCTTTTCCACCACAATGGACAGCACTTGTGGACAGCACTTGTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

145

Amino Acids

16.69

Weight (kDa)

7.91

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
AccB7I CCANNNNNTGG 1 cut(s) 202
AclWI GGATC 2 cut(s) 11, 50
AcuI CTGAAG 1 cut(s) 119
AcvI CACGTG 1 cut(s) 178
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 202, 222, 245
AflIII ACRYGT 2 cut(s) 175, 177
AgsI TTSAA 1 cut(s) 144
AjnI CCWGG 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 2 cut(s) 11, 50
AoxI GGCC 2 cut(s) 204, 282
ApeKI GCWGC 2 cut(s) 73, 212
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 226
AxyI CCTNAGG 1 cut(s) 38
BanII GRGCYC 1 cut(s) 115
BbrPI CACGTG 1 cut(s) 178
BbvI GCAGC 2 cut(s) 85, 224
BccI CCATC 4 cut(s) 14, 73, 239, 311
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 166
BfmI CTRYAG 1 cut(s) 431
BisI GCNGC 2 cut(s) 74, 213
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 75, 214
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 3 cut(s) 90, 194, 227
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 54
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 222, 245
Bse21I CCTNAGG 1 cut(s) 38
BseBI CCWGG 1 cut(s) 203
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 3 cut(s) 202, 222, 245
BseMII CTCAG 3 cut(s) 52, 128, 243
BseXI GCAGC 2 cut(s) 85, 224
BshFI GGCC 2 cut(s) 206, 284
BslI CCNNNNNNNGG 3 cut(s) 202, 222, 245
BsmAI GTCTC 1 cut(s) 166
BsnI GGCC 2 cut(s) 206, 284
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 16, 42
Bsp1720I GCTNAGC 1 cut(s) 114
BspANI GGCC 2 cut(s) 206, 284
BspCNI CTCAG 3 cut(s) 51, 127, 244
BspLI GGNNCC 3 cut(s) 90, 194, 227
BspPI GGATC 2 cut(s) 11, 50
BssMI GATC 2 cut(s) 16, 42
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 178
BstDEI CTNAG 4 cut(s) 38, 114, 234, 252
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 2 cut(s) 19, 45
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 2 cut(s) 16, 42
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 431
BstV1I GCAGC 2 cut(s) 85, 224
BstX2I RGATCY 1 cut(s) 16
BstXI CCANNNNNNTGG 1 cut(s) 402
BstYI RGATCY 1 cut(s) 16
Bsu36I CCTNAGG 1 cut(s) 38
BsuRI GGCC 2 cut(s) 206, 284
BtsCI GGATG 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 226
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 160
CviAII CATG 3 cut(s) 83, 197, 386
CviJI RGCY 7 cut(s) 113, 206, 215, 238, 249, 266, 284
CviKI_1 RGCY 7 cut(s) 113, 206, 215, 238, 249, 266, 284
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 4 cut(s) 38, 114, 234, 252
DpnI GATC 2 cut(s) 18, 44
DpnII GATC 2 cut(s) 16, 42
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 119
Eco72I CACGTG 1 cut(s) 178
Eco81I CCTNAGG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 115
FaeI CATG 3 cut(s) 86, 200, 389
FaiI YATR 8 cut(s) 84, 96, 123, 198, 279, 324, 387, 433
FalI AAGNNNNNCTT 2 cut(s) 373, 405
FatI CATG 3 cut(s) 82, 196, 385
Fnu4HI GCNGC 2 cut(s) 74, 213
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 2 cut(s) 74, 213
GluI GCNGC 2 cut(s) 74, 213
HaeIII GGCC 2 cut(s) 206, 284
HgaI GACGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 3 cut(s) 86, 200, 389
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 3 cut(s) 138, 171, 253
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 3 cut(s) 143, 265, 293
HpyCH4IV ACGT 2 cut(s) 54, 177
HpyCH4V TGCA 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 4 cut(s) 38, 114, 234, 252
HpySE526I ACGT 2 cut(s) 54, 177
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 3 cut(s) 86, 200, 389
Kzo9I GATC 2 cut(s) 16, 42
LpnPI CCDG 5 cut(s) 25, 174, 188, 215, 242
Lsp1109I GCAGC 2 cut(s) 85, 224
LweI GCATC 1 cut(s) 127
MaeII ACGT 2 cut(s) 54, 177
MalI GATC 2 cut(s) 18, 44
MboI GATC 2 cut(s) 16, 42
MboII GAAGA 1 cut(s) 302
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 1 cut(s) 330
MnlI CCTC 4 cut(s) 47, 79, 163, 274
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 400
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 212
NdeII GATC 2 cut(s) 16, 42
NlaIII CATG 3 cut(s) 86, 200, 389
NlaIV GGNNCC 3 cut(s) 90, 194, 227
PflFI GACNNNGTC 1 cut(s) 56
PflMI CCANNNNNTGG 1 cut(s) 202
PkrI GCNGC 2 cut(s) 75, 214
PmaCI CACGTG 1 cut(s) 178
PmlI CACGTG 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 178
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 201
PspCI CACGTG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 3 cut(s) 90, 194, 227
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PsuI RGATCY 1 cut(s) 16
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 400
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 74, 213
Sau3AI GATC 2 cut(s) 16, 42
Sau96I GGNCC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 115
SetI ASST 5 cut(s) 39, 57, 110, 180, 204
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 431
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 400
Sse9I AATT 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 201
TaiI ACGT 2 cut(s) 57, 180
TaqI TCGA 2 cut(s) 68, 128
TasI AATT 1 cut(s) 330
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseI GCWGC 2 cut(s) 73, 212
TspDTI ATGAA 1 cut(s) 303
Tth111I GACNNNGTC 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 226
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.