RchiOBHm_Chr3g0472431

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000803
Physical Location & Seq
Reverse (-)
18338172 .. 18341403
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43814

Sequence Viewer

Length: 930 bp
ATGTCGGACCCAAAACCCAGACAAGTCTGCTGCATCGGCGACGTCCACGGCTTCCACACGAAGCTCCAAAACCTCTGGTCCAATCTCGAATCCTCCATCCCCTCTTCAGACTTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGCGACCGCGGCCCAGACACCAACAAAGTCCTCGACTTCCTCATTTCCCTGCCCTCCAGATACCCGCATCAGAAACACGTCTTCATCGCCGGAAACCACGACTTCGCCTTCGCCGGCTTCTTGAGAGTCCTGCCTCCGCCGGCGGACGGGTCCGAGTTCCGGGAGGGGTGGAAGGAGTTCGAGGACAATGAGGAGAGAGAAGGGTGGTTCAAGGCCGAGGGGTACGAGGGAATGCACTTGCAAGGGAGGAGATGGGCTGGGAGTATGAAGGGGTTTAATAAGGCTAAGGGGACCGAGTACCAGGGTTCCATTTATGACGCTGGGCCCACTTTTGAGTCTTATGGGGTGCCTCATGGCTCTGCCGATCTGATGAAGGCAGTTCCTGATGATCATAAGAAGTTTCTTGCCGATCTGGTTTGGGTGCATGAAGAGGATGATGTCTGTGTAGAGATTGAGGGTGAAACCAAACGCTGTAAATTGATTGCCGTGCACGCCGGTTTGGAGAAAGATAAAGATGTCAAACAACAGTTGGAGTTTCTGAAAGCCCGAGATACTCGGGTACCTAAGATAGAGGCTCTGAGTGGCAGGAAAGACGTGTGGAATATACCAGAGGAACTGAAAAAGACTCCAACTGTTATTGTAAGTGGACATCATGGGAAGCTTCATATTGAAGGCTTAAGGCTGATTATTGATGAAAGCGGTGGTATGGCAAAGAACCCGGTGGCTGCAATTTTACTCCCCTCTATGAGGATAGTCCGTGATACAGACATTTTGGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

309

Amino Acids

34.63

Weight (kDa)

5.82

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 9 - 162 4.3e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 2 cut(s) 493, 706
AccII CGCG 1 cut(s) 156
AciI CCGC 7 cut(s) 120, 154, 156, 212, 284, 290, 846
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 371, 446, 708
AfiI CCNNNNNNNGG 3 cut(s) 293, 306, 894
AflII CTTAAG 1 cut(s) 823
AflIII ACRYGT 2 cut(s) 223, 741
AgsI TTSAA 2 cut(s) 358, 818
AjiI CACGTC 2 cut(s) 226, 742
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 2 cut(s) 64, 808
AluI AGCT 2 cut(s) 64, 808
Alw21I GWGCWC 1 cut(s) 639
Alw44I GTGCAC 1 cut(s) 635
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 2 cut(s) 693, 702
AoxI GGCC 3 cut(s) 157, 360, 470
ApaI GGGCCC 1 cut(s) 474
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 2 cut(s) 30, 872
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 1 cut(s) 323
Asp718I GGTACC 1 cut(s) 706
AspS9I GGNCC 7 cut(s) 7, 78, 158, 297, 438, 470, 471
AsuC2I CCSGG 2 cut(s) 308, 866
AsuHPI GGTGA 1 cut(s) 617
AvaI CYCGRG 2 cut(s) 693, 702
AvaII GGWCC 4 cut(s) 7, 78, 297, 438
BaeGI GKGCMC 2 cut(s) 474, 639
BaeI ACNNNNGTAYC 4 cut(s) 690, 690, 723, 723
BanI GGYRCC 2 cut(s) 493, 706
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 17, 859
BccI CCATC 2 cut(s) 104, 393
BceAI ACGGC 2 cut(s) 64, 617
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 308, 866
BfaI CTAG 1 cut(s) 928
BfrI CTTAAG 1 cut(s) 823
BisI GCNGC 3 cut(s) 31, 157, 873
BlsI GCNGC 3 cut(s) 32, 158, 874
Bme1390I CCNGG 3 cut(s) 308, 449, 866
Bme18I GGWCC 4 cut(s) 7, 78, 297, 438
BmeT110I CYCGRG 2 cut(s) 693, 702
BmgBI CACGTC 2 cut(s) 226, 742
BmgT120I GGNCC 7 cut(s) 7, 78, 158, 297, 438, 470, 471
BmiI GGNNCC 7 cut(s) 9, 298, 439, 454, 472, 495, 708
BmrFI CCNGG 3 cut(s) 308, 449, 866
BmsI GCATC 2 cut(s) 42, 223
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 2 cut(s) 308, 866
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 5 cut(s) 46, 136, 154, 363, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 306, 894
Bse118I RCCGGY 3 cut(s) 260, 286, 641
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 46, 136, 154, 363, 448
BseGI GGATG 2 cut(s) 96, 586
BseLI CCNNNNNNNGG 3 cut(s) 293, 306, 894
BseMII CTCAG 1 cut(s) 716
BseRI GAGGAG 2 cut(s) 353, 409
BseSI GKGCMC 2 cut(s) 474, 639
BseXI GCAGC 2 cut(s) 17, 859
BseYI CCCAGC 2 cut(s) 404, 467
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 159, 362, 472
BshNI GGYRCC 2 cut(s) 493, 706
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 2 cut(s) 693, 702
BsiSI CCGG 6 cut(s) 237, 261, 287, 307, 642, 866
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 3 cut(s) 293, 306, 894
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 3 cut(s) 159, 362, 472
BsoBI CYCGRG 2 cut(s) 693, 702
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 474, 639
Bsp143I GATC 3 cut(s) 511, 535, 556
BspACI CCGC 7 cut(s) 120, 154, 156, 212, 284, 290, 846
BspANI GGCC 3 cut(s) 159, 362, 472
BspCNI CTCAG 1 cut(s) 717
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 7 cut(s) 9, 298, 439, 454, 472, 495, 708
BspT107I GGYRCC 2 cut(s) 493, 706
BspTI CTTAAG 1 cut(s) 823
BsrFI RCCGGY 3 cut(s) 260, 286, 641
BssAI RCCGGY 3 cut(s) 260, 286, 641
BssECI CCNNGG 5 cut(s) 46, 136, 154, 363, 448
BssMI GATC 3 cut(s) 511, 535, 556
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 2 cut(s) 675, 781
Bst6I CTCTTC 2 cut(s) 109, 570
BstACI GRCGYC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 823
BstC8I GCNNGC 3 cut(s) 262, 288, 639
BstDEI CTNAG 3 cut(s) 432, 711, 725
BstDSI CCRYGG 2 cut(s) 46, 154
BstENI CCTNNNNNAGG 1 cut(s) 892
BstF5I GGATG 2 cut(s) 96, 586
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 3 cut(s) 514, 538, 559
BstMBI GATC 3 cut(s) 511, 535, 556
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 4 cut(s) 36, 147, 156, 638
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 3 cut(s) 306, 447, 864
BstSLI GKGCMC 2 cut(s) 474, 639
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 17, 859
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 3 cut(s) 159, 362, 472
BtgI CCRYGG 2 cut(s) 46, 154
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 2 cut(s) 226, 742
BtsCI GGATG 2 cut(s) 96, 586
Cac8I GCNNGC 3 cut(s) 262, 288, 639
CaiI CAGNNNCTG 1 cut(s) 530
Cfr10I RCCGGY 3 cut(s) 260, 286, 641
Cfr13I GGNCC 7 cut(s) 7, 78, 158, 297, 438, 470, 471
Cfr42I CCGCGG 1 cut(s) 157
CseI GACGC 1 cut(s) 473
Csp6I GTAC 3 cut(s) 370, 445, 707
CviAII CATG 3 cut(s) 500, 572, 800
CviQI GTAC 3 cut(s) 370, 445, 707
DdeI CTNAG 3 cut(s) 432, 711, 725
DpnI GATC 3 cut(s) 513, 537, 558
DpnII GATC 3 cut(s) 511, 535, 556
Eam1104I CTCTTC 2 cut(s) 109, 570
EarI CTCTTC 2 cut(s) 109, 570
EciI GGCGGA 2 cut(s) 273, 305
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 4 cut(s) 7, 78, 297, 438
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 2 cut(s) 693, 702
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoRII CCWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 3 cut(s) 503, 575, 803
FaqI GGGAC 1 cut(s) 451
FatI CATG 3 cut(s) 499, 571, 799
FauI CCCGC 1 cut(s) 219
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 31, 157, 873
FokI GGATG 2 cut(s) 83, 593
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 31, 157, 873
FspBI CTAG 1 cut(s) 928
GluI GCNGC 3 cut(s) 31, 157, 873
GsaI CCCAGC 2 cut(s) 408, 471
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 3 cut(s) 159, 362, 472
HapII CCGG 6 cut(s) 237, 261, 287, 307, 642, 866
HgaI GACGC 1 cut(s) 473
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 3 cut(s) 503, 575, 803
HindIII AAGCTT 1 cut(s) 806
HinfI GANTC 4 cut(s) 89, 273, 482, 772
HpaII CCGG 6 cut(s) 237, 261, 287, 307, 642, 866
HphI GGTGA 1 cut(s) 617
Hpy166II GTNNAC 3 cut(s) 46, 637, 794
Hpy188I TCNGA 7 cut(s) 7, 109, 219, 301, 516, 687, 726
Hpy188III TCNNGA 4 cut(s) 86, 204, 268, 530
Hpy8I GTNNAC 3 cut(s) 46, 637, 794
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 6 cut(s) 265, 313, 341, 409, 514, 812
HpyCH4III ACNGT 2 cut(s) 675, 781
HpyCH4IV ACGT 3 cut(s) 42, 225, 741
HpyCH4V TGCA 6 cut(s) 33, 382, 388, 571, 637, 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 147, 156, 638
HpyF3I CTNAG 3 cut(s) 432, 711, 725
HpySE526I ACGT 3 cut(s) 42, 225, 741
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 3 cut(s) 503, 575, 803
KpnI GGTACC 1 cut(s) 710
KroI GCCGGC 2 cut(s) 260, 286
KroNI GCCGGC 2 cut(s) 262, 288
Ksp22I TGATCA 1 cut(s) 535
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 3 cut(s) 511, 535, 556
LmnI GCTCC 1 cut(s) 69
Lsp1109I GCAGC 2 cut(s) 17, 859
LweI GCATC 2 cut(s) 42, 223
MaeI CTAG 1 cut(s) 928
MaeII ACGT 3 cut(s) 42, 225, 741
MalI GATC 3 cut(s) 513, 537, 558
MboI GATC 3 cut(s) 511, 535, 556
MboII GAAGA 4 cut(s) 96, 124, 220, 587
MhlI GDGCHC 2 cut(s) 474, 639
MluCI AATT 2 cut(s) 623, 876
MlyI GAGTC 3 cut(s) 282, 491, 766
MmeI TCCRAC 2 cut(s) 657, 800
MreI CGCCGGCG 1 cut(s) 286
MroNI GCCGGC 2 cut(s) 260, 286
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 2 cut(s) 423, 824
MspA1I CMGCKG 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 6 cut(s) 237, 261, 287, 307, 642, 866
MspR9I CCNGG 3 cut(s) 308, 449, 866
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 4 cut(s) 36, 147, 156, 638
NaeI GCCGGC 2 cut(s) 262, 288
NciI CCSGG 2 cut(s) 308, 866
NdeII GATC 3 cut(s) 511, 535, 556
NgoMIV GCCGGC 2 cut(s) 260, 286
NlaIII CATG 3 cut(s) 503, 575, 803
NlaIV GGNNCC 7 cut(s) 9, 298, 439, 454, 472, 495, 708
NmeAIII GCCGAG 2 cut(s) 117, 388
PctI GAATGC 1 cut(s) 384
PdiI GCCGGC 2 cut(s) 262, 288
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 3 cut(s) 32, 158, 874
PleI GAGTC 3 cut(s) 281, 490, 766
PpsI GAGTC 3 cut(s) 281, 490, 766
Psp6I CCWGG 1 cut(s) 447
PspFI CCCAGC 2 cut(s) 404, 467
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 7 cut(s) 9, 298, 439, 454, 472, 495, 708
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 7 cut(s) 7, 78, 158, 297, 438, 470, 471
PstNI CAGNNNCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 371, 446, 708
RsaNI GTAC 3 cut(s) 370, 445, 707
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 2 cut(s) 423, 824
SatI GCNGC 3 cut(s) 31, 157, 873
Sau3AI GATC 3 cut(s) 511, 535, 556
Sau96I GGNCC 7 cut(s) 7, 78, 158, 297, 438, 470, 471
SchI GAGTC 3 cut(s) 282, 491, 766
ScrFI CCNGG 3 cut(s) 308, 449, 866
SduI GDGCHC 2 cut(s) 474, 639
SetI ASST 7 cut(s) 45, 66, 75, 228, 712, 744, 810
SfaNI GCATC 2 cut(s) 42, 223
Sfr303I CCGCGG 1 cut(s) 157
SgrAI CRCCGGYG 1 cut(s) 286
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 4 cut(s) 7, 78, 297, 438
SmlI CTYRAG 2 cut(s) 268, 823
SmoI CTYRAG 2 cut(s) 268, 823
Sse9I AATT 2 cut(s) 623, 876
SsiI CCGC 7 cut(s) 120, 154, 156, 212, 284, 290, 846
SspMI CTAG 1 cut(s) 928
StyD4I CCNGG 3 cut(s) 306, 447, 864
TaaI ACNGT 2 cut(s) 675, 781
TaiI ACGT 3 cut(s) 45, 228, 744
TaqI TCGA 3 cut(s) 87, 180, 327
TaqII GACCGA 1 cut(s) 455
TasI AATT 2 cut(s) 623, 876
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 423, 824
Tru9I TTAA 2 cut(s) 423, 824
TseI GCWGC 2 cut(s) 30, 872
TspDTI ATGAA 6 cut(s) 220, 428, 533, 588, 800, 855
TspGWI ACGGA 1 cut(s) 893
Vha464I CTTAAG 1 cut(s) 823
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 4 cut(s) 7, 78, 297, 438
XagI CCTNNNNNAGG 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 928
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.