Rroxscaffold_6G00408910

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000803
Physical Location & Seq
Forward (+)
31435790 .. 31438694
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408910.1

Sequence Viewer

Length: 930 bp
ATGTCAGACCCAAAACCCAGACTAGTCTGCTGCATCGGCGACGTCCACGGCTTCCACACCAAGCTCCAAAACCTCTGGTCCAATCTCGAAATCTCCATCCCCTCTTCAGACTTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGCGACCGCGGCCCAGACACCAACAAAGTCCTCGACTTCCTCATTTCCCTCCCCTCCCGATACCCGCATCAGAAACACGTCTTCATCGCCGGAAACCACGACTTCGCCTTCGCCGCCTTCTTGGGAGTCCTGCCTCCGCCGCCGGACGGGTCCGAGTTCCGGGAGGAGTGGAAGGAGTTCGAGGACAATGAGGAGAGAGAAGGGTGGTTCAAGGGCGAGGGGTACGAGGGAATGCACTTGCAGGGGAGGAGATGGGCTGGGAGTTCGAAGGGGTTTAATAAGGCTAAAGGGACTGAGTACCAGGGTTCCGTTTATAAAGCTGGGCCCACTTTTGAGTCTTATGGAGTGCCCCATGGCTCTGCCGATCTGGTGAAGGCAGTTCCTGATAATCATAAGAAGTTTCTTGCCGATATGGTTTGGGTGCATGAAGAGGATGATGTCTGTGTAGAGATTGAGGGTGAAACCAAACACTGTAAATTGATTGCCGTGCATGCCGGTTTGGAGAAAGATAAAGATGTCAAACAACAGTTGGAGTTTCTGAAAGCCAGAGATACTCGGGTACCTAAGATAAGGGCTCTGAGTGGCAGGAAAGACGTGTGGAATATACCAGAGGAACTGAAAAAGACTCAAACTGTTGTTGTAAGTGGACATCATGGGAAACTTCATATTGAAGGCTTAAGGCTGATTATTGATGAAGGCGATGGTATGGCAAAGAACCCAGTGGCTGCAATTTTACTCCCCTCTATGAAAATAGTCCGTGATACAGACGTTTTGGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

309

Amino Acids

34.6

Weight (kDa)

5.97

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 9 - 112 1.5e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 462
AatII GACGTC 1 cut(s) 45
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 1 cut(s) 706
AccII CGCG 1 cut(s) 156
AciI CCGC 7 cut(s) 120, 154, 156, 212, 261, 284, 287
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 371, 446, 708
AfiI CCNNNNNNNGG 2 cut(s) 293, 306
AflII CTTAAG 1 cut(s) 823
AflIII ACRYGT 2 cut(s) 223, 741
AgsI TTSAA 2 cut(s) 358, 818
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 2 cut(s) 226, 742
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 2 cut(s) 64, 467
AluI AGCT 2 cut(s) 64, 467
AlwNI CAGNNNCTG 2 cut(s) 530, 872
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 2 cut(s) 157, 470
ApaI GGGCCC 1 cut(s) 474
ApeKI GCWGC 2 cut(s) 30, 872
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 1 cut(s) 323
Asp718I GGTACC 1 cut(s) 706
AspS9I GGNCC 5 cut(s) 78, 158, 297, 470, 471
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 2 cut(s) 529, 617
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 2 cut(s) 78, 297
BaeGI GKGCMC 2 cut(s) 474, 498
BaeI ACNNNNGTAYC 5 cut(s) 690, 690, 723, 723, 900
BanI GGYRCC 1 cut(s) 706
BanII GRGCYC 2 cut(s) 474, 724
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 17, 859
BccI CCATC 3 cut(s) 104, 393, 842
BceAI ACGGC 2 cut(s) 64, 617
BciT130I CCWGG 1 cut(s) 449
BcnI CCSGG 1 cut(s) 308
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 928
BfrI CTTAAG 1 cut(s) 823
BisI GCNGC 5 cut(s) 31, 157, 261, 287, 873
BlsI GCNGC 5 cut(s) 32, 158, 262, 288, 874
Bme1390I CCNGG 2 cut(s) 308, 449
Bme18I GGWCC 2 cut(s) 78, 297
BmeT110I CYCGRG 1 cut(s) 702
BmgBI CACGTC 2 cut(s) 226, 742
BmgT120I GGNCC 5 cut(s) 78, 158, 297, 470, 471
BmiI GGNNCC 4 cut(s) 298, 454, 472, 708
BmrFI CCNGG 2 cut(s) 308, 449
BmrI ACTGGG 1 cut(s) 860
BmsI GCATC 2 cut(s) 42, 223
BmuI ACTGGG 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 220
Bpu14I TTCGAA 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 5 cut(s) 46, 136, 154, 448, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 306
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 866
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 46, 136, 154, 448, 499
BseGI GGATG 2 cut(s) 96, 586
BseLI CCNNNNNNNGG 2 cut(s) 293, 306
BseMII CTCAG 2 cut(s) 432, 716
BseNI ACTGG 1 cut(s) 866
BseRI GAGGAG 3 cut(s) 326, 353, 409
BseSI GKGCMC 2 cut(s) 474, 498
BseXI GCAGC 2 cut(s) 17, 859
BseYI CCCAGC 2 cut(s) 404, 467
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 159, 472
BshNI GGYRCC 1 cut(s) 706
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 4 cut(s) 237, 290, 307, 642
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 2 cut(s) 293, 306
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 2 cut(s) 159, 472
BsoBI CYCGRG 1 cut(s) 702
Bsp119I TTCGAA 1 cut(s) 413
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 3 cut(s) 474, 498, 724
Bsp143I GATC 1 cut(s) 511
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 7 cut(s) 120, 154, 156, 212, 261, 284, 287
BspANI GGCC 2 cut(s) 159, 472
BspCNI CTCAG 2 cut(s) 433, 717
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 4 cut(s) 298, 454, 472, 708
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 706
BspTI CTTAAG 1 cut(s) 823
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 1 cut(s) 866
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 5 cut(s) 46, 136, 154, 448, 499
BssMI GATC 1 cut(s) 511
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 499
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 620, 675, 781
Bst6I CTCTTC 2 cut(s) 109, 570
BstACI GRCGYC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 823
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 3 cut(s) 441, 711, 725
BstDSI CCRYGG 3 cut(s) 46, 154, 499
BstF5I GGATG 2 cut(s) 96, 586
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 1 cut(s) 514
BstMBI GATC 1 cut(s) 511
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 6 cut(s) 36, 147, 156, 260, 286, 638
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 2 cut(s) 306, 447
BstSLI GKGCMC 2 cut(s) 474, 498
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 17, 859
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 2 cut(s) 159, 472
BtgI CCRYGG 3 cut(s) 46, 154, 499
BtgZI GCGATG 2 cut(s) 217, 861
BtrI CACGTC 2 cut(s) 226, 742
BtsCI GGATG 2 cut(s) 96, 586
BtsIMutI CAGTG 2 cut(s) 616, 873
Cac8I GCNNGC 1 cut(s) 639
CaiI CAGNNNCTG 2 cut(s) 530, 872
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 5 cut(s) 78, 158, 297, 470, 471
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 3 cut(s) 370, 445, 707
CviAII CATG 4 cut(s) 500, 572, 638, 800
CviQI GTAC 3 cut(s) 370, 445, 707
DdeI CTNAG 3 cut(s) 441, 711, 725
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
Eam1104I CTCTTC 2 cut(s) 109, 570
EarI CTCTTC 2 cut(s) 109, 570
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 2 cut(s) 474, 724
Eco47I GGWCC 2 cut(s) 78, 297
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 499
EcoT38I GRGCYC 2 cut(s) 474, 724
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 4 cut(s) 503, 575, 641, 803
FaqI GGGAC 1 cut(s) 451
FatI CATG 4 cut(s) 499, 571, 637, 799
FauI CCCGC 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 31, 157, 261, 287, 873
FokI GGATG 2 cut(s) 83, 593
FriOI GRGCYC 2 cut(s) 474, 724
Fsp4HI GCNGC 5 cut(s) 31, 157, 261, 287, 873
FspBI CTAG 2 cut(s) 23, 928
GluI GCNGC 5 cut(s) 31, 157, 261, 287, 873
GsaI CCCAGC 2 cut(s) 408, 471
HaeIII GGCC 2 cut(s) 159, 472
HapII CCGG 4 cut(s) 237, 290, 307, 642
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 4 cut(s) 503, 575, 641, 803
HinfI GANTC 3 cut(s) 273, 482, 772
HpaII CCGG 4 cut(s) 237, 290, 307, 642
HphI GGTGA 2 cut(s) 529, 617
Hpy166II GTNNAC 2 cut(s) 46, 794
Hpy188I TCNGA 6 cut(s) 7, 109, 219, 301, 687, 726
Hpy188III TCNNGA 3 cut(s) 86, 204, 530
Hpy8I GTNNAC 2 cut(s) 46, 794
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 8 cut(s) 265, 274, 313, 341, 409, 514, 812, 836
HpyCH4III ACNGT 3 cut(s) 620, 675, 781
HpyCH4IV ACGT 4 cut(s) 42, 225, 741, 915
HpyCH4V TGCA 6 cut(s) 33, 382, 388, 571, 637, 875
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 147, 156, 260, 286, 638
HpyF3I CTNAG 3 cut(s) 441, 711, 725
HpySE526I ACGT 4 cut(s) 42, 225, 741, 915
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 4 cut(s) 503, 575, 641, 803
KpnI GGTACC 1 cut(s) 710
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 1 cut(s) 511
LmnI GCTCC 1 cut(s) 69
Lsp1109I GCAGC 2 cut(s) 17, 859
LweI GCATC 2 cut(s) 42, 223
MaeI CTAG 2 cut(s) 23, 928
MaeII ACGT 4 cut(s) 42, 225, 741, 915
MalI GATC 1 cut(s) 513
MboI GATC 1 cut(s) 511
MboII GAAGA 4 cut(s) 96, 124, 220, 587
MhlI GDGCHC 3 cut(s) 474, 498, 724
MluCI AATT 2 cut(s) 623, 876
MlyI GAGTC 3 cut(s) 282, 491, 766
MmeI TCCRAC 1 cut(s) 657
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 2 cut(s) 423, 824
MspA1I CMGCKG 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 4 cut(s) 237, 290, 307, 642
MspR9I CCNGG 2 cut(s) 308, 449
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 6 cut(s) 36, 147, 156, 260, 286, 638
NciI CCSGG 1 cut(s) 308
NcoI CCATGG 1 cut(s) 499
NdeII GATC 1 cut(s) 511
NlaIII CATG 4 cut(s) 503, 575, 641, 803
NlaIV GGNNCC 4 cut(s) 298, 454, 472, 708
NmeAIII GCCGAG 1 cut(s) 117
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 413
PaeI GCATGC 1 cut(s) 641
PctI GAATGC 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 5 cut(s) 32, 158, 262, 288, 874
PleI GAGTC 3 cut(s) 281, 490, 766
PpsI GAGTC 3 cut(s) 281, 490, 766
PsiI TTATAA 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 447
PspFI CCCAGC 2 cut(s) 404, 467
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 4 cut(s) 298, 454, 472, 708
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 5 cut(s) 78, 158, 297, 470, 471
PstNI CAGNNNCTG 2 cut(s) 530, 872
RsaI GTAC 3 cut(s) 371, 446, 708
RsaNI GTAC 3 cut(s) 370, 445, 707
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 2 cut(s) 423, 824
SatI GCNGC 5 cut(s) 31, 157, 261, 287, 873
Sau3AI GATC 1 cut(s) 511
Sau96I GGNCC 5 cut(s) 78, 158, 297, 470, 471
SchI GAGTC 3 cut(s) 282, 491, 766
ScrFI CCNGG 2 cut(s) 308, 449
SduI GDGCHC 3 cut(s) 474, 498, 724
SetI ASST 8 cut(s) 45, 66, 75, 228, 469, 712, 744, 918
SfaNI GCATC 2 cut(s) 42, 223
Sfr303I CCGCGG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 2 cut(s) 78, 297
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
SpeI ACTAGT 1 cut(s) 22
SphI GCATGC 1 cut(s) 641
Sse9I AATT 2 cut(s) 623, 876
SsiI CCGC 7 cut(s) 120, 154, 156, 212, 261, 284, 287
SspMI CTAG 2 cut(s) 23, 928
StyD4I CCNGG 2 cut(s) 306, 447
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 3 cut(s) 620, 675, 781
TaiI ACGT 4 cut(s) 45, 228, 744, 918
TaqI TCGA 4 cut(s) 87, 180, 327, 413
TasI AATT 2 cut(s) 623, 876
TauI GCSGC 3 cut(s) 159, 263, 289
Tru1I TTAA 2 cut(s) 423, 824
Tru9I TTAA 2 cut(s) 423, 824
TscAI CASTG 2 cut(s) 623, 873
TseI GCWGC 2 cut(s) 30, 872
TspDTI ATGAA 5 cut(s) 220, 588, 800, 855, 908
TspGWI ACGGA 2 cut(s) 445, 893
TspRI CASTG 2 cut(s) 623, 873
Vha464I CTTAAG 1 cut(s) 823
VpaK11BI GGWCC 2 cut(s) 78, 297
XceI RCATGY 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 2 cut(s) 23, 928
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.