Rh7BG062000

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000803
Physical Location & Seq
Reverse (-)
4122933 .. 4123343
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG062000.1

Sequence Viewer

Length: 411 bp
ATGTCAGACCCAAAACCCAGACTAGTCTGTTGCATCGGCGACGTCCACGGCTTCCACACCAAGCTCCAAAATCTCTGGTCCAATCTCGAAATCTCCATCCCTTCTTCAGACTTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGCGGCCGCAGCCCTGACACCAACGAAGTCCTTGACTTCTTCGTCGCTCTCCCCTCCAGATACCCACGTCAGAAAGACGTCTTCATCGCCGGAAACCATGACTTCGCCTTCACCGCCTTGTTGAGAGTCCTGCCTCTGCCGCCGGACGGGTTCGAGTTCCGCGGGGGGTGGAAGGAGTTCGAGGACAAGGAGGAGAGAGAAAAAGATCTGGAGACAGAGAGAGGGCAAGAAGATGTAAAAAGAAAAAGAGACAAAATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

136

Amino Acids

15.66

Weight (kDa)

5.41

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 9 - 110 3.9e-11 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AatII GACGTC 2 cut(s) 45, 228
AccII CGCG 1 cut(s) 309
AciI CCGC 7 cut(s) 120, 150, 154, 261, 287, 307, 309
AcoI YGGCCR 1 cut(s) 151
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 2 cut(s) 42, 225
AfiI CCNNNNNNNGG 1 cut(s) 293
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 1 cut(s) 215
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw26I GTCTC 2 cut(s) 353, 390
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 156
Asp700I GAANNNNTTC 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 250
AvaII GGWCC 1 cut(s) 78
BaeI ACNNNNGTAYC 2 cut(s) 199, 232
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 168
BccI CCATC 1 cut(s) 104
BceAI ACGGC 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 353, 390
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 1 cut(s) 23
BglII AGATCT 1 cut(s) 352
BisI GCNGC 4 cut(s) 151, 154, 157, 287
BlsI GCNGC 4 cut(s) 152, 155, 158, 288
Bme18I GGWCC 1 cut(s) 78
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 78
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 2 cut(s) 187, 377
BsaHI GRCGYC 2 cut(s) 42, 225
BsaJI CCNNGG 3 cut(s) 46, 136, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 46, 136, 307
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 353
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 237, 290
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 2 cut(s) 353, 390
BsnI GGCC 1 cut(s) 153
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 7 cut(s) 120, 150, 154, 261, 287, 307, 309
BspANI GGCC 1 cut(s) 153
BspFNI CGCG 1 cut(s) 309
BssECI CCNNGG 3 cut(s) 46, 136, 307
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 2 cut(s) 42, 225
BstACI GRCGYC 2 cut(s) 42, 225
BstDSI CCRYGG 2 cut(s) 46, 307
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 309
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 2 cut(s) 353, 390
BstMBI GATC 1 cut(s) 352
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 4 cut(s) 147, 156, 260, 286
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BstZI CGGCCG 1 cut(s) 151
BsuRI GGCC 1 cut(s) 153
BtgI CCRYGG 2 cut(s) 46, 307
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 1 cut(s) 96
CciNI GCGGCCGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 78
Cfr42I CCGCGG 1 cut(s) 310
CviAII CATG 1 cut(s) 245
CviJI RGCY 4 cut(s) 51, 64, 153, 159
CviKI_1 RGCY 4 cut(s) 51, 64, 153, 159
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 151
EagI CGGCCG 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 90
FaeI CATG 1 cut(s) 248
FaiI YATR 1 cut(s) 246
FatI CATG 1 cut(s) 244
FauI CCCGC 1 cut(s) 302
Fnu4HI GCNGC 4 cut(s) 151, 154, 157, 287
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 151, 154, 157, 287
FspBI CTAG 1 cut(s) 23
GluI GCNGC 4 cut(s) 151, 154, 157, 287
GsuI CTGGAG 2 cut(s) 187, 377
HaeIII GGCC 1 cut(s) 153
HapII CCGG 2 cut(s) 237, 290
Hin1I GRCGYC 2 cut(s) 42, 225
Hin1II CATG 1 cut(s) 248
HinfI GANTC 1 cut(s) 273
HpaII CCGG 2 cut(s) 237, 290
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 7, 109, 219
Hpy188III TCNNGA 3 cut(s) 86, 204, 356
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 2 cut(s) 44, 194
HpyAV CCTTC 3 cut(s) 111, 265, 313
HpyCH4IV ACGT 3 cut(s) 42, 214, 225
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 156, 260, 286
HpySE526I ACGT 3 cut(s) 42, 214, 225
Hsp92I GRCGYC 2 cut(s) 42, 225
Hsp92II CATG 1 cut(s) 248
KspI CCGCGG 1 cut(s) 310
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 8 cut(s) 31, 61, 174, 217, 250, 290, 303, 341
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 23
MaeII ACGT 3 cut(s) 42, 214, 225
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 5 cut(s) 96, 124, 178, 220, 389
MflI RGATCY 1 cut(s) 352
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 7 cut(s) 134, 146, 211, 291, 322, 331, 362
MroXI GAANNNNTTC 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 2 cut(s) 237, 290
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 4 cut(s) 147, 156, 260, 286
NdeII GATC 1 cut(s) 352
NlaIII CATG 1 cut(s) 248
NmeAIII GCCGAG 1 cut(s) 117
NotI GCGGCCGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 323
PkrI GCNGC 4 cut(s) 152, 155, 158, 288
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PspPI GGNCC 1 cut(s) 78
PsuI RGATCY 1 cut(s) 352
SacII CCGCGG 1 cut(s) 310
SatI GCNGC 4 cut(s) 151, 154, 157, 287
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 1 cut(s) 282
SetI ASST 4 cut(s) 45, 66, 217, 228
SfaNI GCATC 1 cut(s) 42
Sfr303I CCGCGG 1 cut(s) 310
SgrBI CCGCGG 1 cut(s) 310
SinI GGWCC 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 22
SsiI CCGC 7 cut(s) 120, 150, 154, 261, 287, 307, 309
SspMI CTAG 1 cut(s) 23
TaiI ACGT 3 cut(s) 45, 217, 228
TaqI TCGA 3 cut(s) 87, 300, 327
TauI GCSGC 3 cut(s) 153, 156, 289
TseI GCWGC 1 cut(s) 156
TspDTI ATGAA 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 23
ZraI GACGTC 2 cut(s) 43, 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.