Rh4CG267100

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000803
Physical Location & Seq
Forward (+)
52135591 .. 52136052
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG267100.1

Sequence Viewer

Length: 462 bp
ATGTCAGACCCAAAACCCAAACTAGTCTGTAGCATCGGCGACGTCCACGGCTTCCACACCAAGCTCCAAAATCTCTGGTCCAATCTCGAAATCTCCATCCCTTCTTCAGACTTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGTGGCAGCGGCCAGGACACCAACGAAGTCCTTGACTTCTTCGTCGCTCTCCCCTCCAGATACCCACATCAGAAAGACGTCTTCATCGCCGGAAACCATGACTTCGCCTTCGCCGCCTTGTTGAGAGTCCTGCCTCTGCCACCGGATGGGTCCGAGTTCCGCGAGGGGTGGAAGGAGTTCGAGGACAAGGAGGAGAGAGAGACAGATCTGGAGATAGAGAGAGGGCAAGAAGATGTAAAAAGAAAGAGAAAAGAAGATAACAAAGAGGAAATCGGAGAAAGATATGTGAGAAAGAGAGAACGATGGAGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

153

Amino Acids

17.8

Weight (kDa)

5.12

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 12 - 111 5e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AatII GACGTC 2 cut(s) 45, 228
AccB7I CCANNNNNTGG 1 cut(s) 293
AccII CGCG 1 cut(s) 309
AciI CCGC 4 cut(s) 120, 156, 261, 307
AcoI YGGCCR 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 2 cut(s) 42, 225
AfiI CCNNNNNNNGG 1 cut(s) 293
AhlI ACTAGT 1 cut(s) 22
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 341
AoxI GGCC 1 cut(s) 157
ApeKI GCWGC 1 cut(s) 153
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 1 cut(s) 323
AspS9I GGNCC 2 cut(s) 78, 297
AvaII GGWCC 2 cut(s) 78, 297
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 165
BccI CCATC 3 cut(s) 104, 287, 444
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 341
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 28
BglII AGATCT 1 cut(s) 352
BisI GCNGC 3 cut(s) 154, 157, 261
BlsI GCNGC 3 cut(s) 155, 158, 262
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 2 cut(s) 78, 297
BmgT120I GGNCC 2 cut(s) 78, 297
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 2 cut(s) 187, 377
BsaHI GRCGYC 2 cut(s) 42, 225
BsaJI CCNNGG 2 cut(s) 46, 136
BsaWI WCCGGW 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 2 cut(s) 46, 136
BseGI GGATG 2 cut(s) 96, 298
BseLI CCNNNNNNNGG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 1 cut(s) 159
BsiSI CCGG 2 cut(s) 237, 290
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 341
BsnI GGCC 1 cut(s) 159
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 4 cut(s) 120, 156, 261, 307
BspANI GGCC 1 cut(s) 159
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 46, 136
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 2 cut(s) 42, 225
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 149
BstACI GRCGYC 2 cut(s) 42, 225
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 2 cut(s) 96, 298
BstFNI CGCG 1 cut(s) 309
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 159
BtgI CCRYGG 1 cut(s) 46
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 2 cut(s) 96, 298
Cfr13I GGNCC 2 cut(s) 78, 297
CviAII CATG 1 cut(s) 245
CviJI RGCY 3 cut(s) 51, 64, 159
CviKI_1 RGCY 3 cut(s) 51, 64, 159
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 78, 297
Eco57I CTGAAG 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 159
FaeI CATG 1 cut(s) 248
FaiI YATR 2 cut(s) 246, 432
FatI CATG 1 cut(s) 244
Fnu4HI GCNGC 3 cut(s) 154, 157, 261
FokI GGATG 2 cut(s) 83, 305
Fsp4HI GCNGC 3 cut(s) 154, 157, 261
FspBI CTAG 1 cut(s) 23
GluI GCNGC 3 cut(s) 154, 157, 261
GsuI CTGGAG 2 cut(s) 187, 377
HaeIII GGCC 1 cut(s) 159
HapII CCGG 2 cut(s) 237, 290
Hin1I GRCGYC 2 cut(s) 42, 225
Hin1II CATG 1 cut(s) 248
HinfI GANTC 1 cut(s) 273
HpaII CCGG 2 cut(s) 237, 290
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 5 cut(s) 7, 109, 219, 301, 422
Hpy188III TCNNGA 3 cut(s) 86, 204, 356
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 2 cut(s) 44, 194
HpyAV CCTTC 3 cut(s) 111, 265, 313
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 2 cut(s) 42, 225
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpySE526I ACGT 2 cut(s) 42, 225
Hsp92I GRCGYC 2 cut(s) 42, 225
Hsp92II CATG 1 cut(s) 248
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 8 cut(s) 61, 146, 173, 217, 250, 290, 303, 341
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 42, 225
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 6 cut(s) 96, 124, 178, 220, 389, 413
MflI RGATCY 1 cut(s) 352
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 9 cut(s) 134, 146, 211, 291, 304, 322, 331, 362, 406
MroXI GAANNNNTTC 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 2 cut(s) 237, 290
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 260
NdeII GATC 1 cut(s) 352
NlaIII CATG 1 cut(s) 248
NlaIV GGNNCC 1 cut(s) 298
NmeAIII GCCGAG 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 3 cut(s) 155, 158, 262
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 2 cut(s) 78, 297
PsuI RGATCY 1 cut(s) 352
SatI GCNGC 3 cut(s) 154, 157, 261
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 2 cut(s) 78, 297
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 1 cut(s) 161
SetI ASST 3 cut(s) 45, 66, 228
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 2 cut(s) 78, 297
SpeI ACTAGT 1 cut(s) 22
SsiI CCGC 4 cut(s) 120, 156, 261, 307
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 2 cut(s) 45, 228
TaqI TCGA 2 cut(s) 87, 327
TauI GCSGC 2 cut(s) 159, 263
TseI GCWGC 1 cut(s) 153
TspDTI ATGAA 1 cut(s) 220
Van91I CCANNNNNTGG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 78, 297
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 23
ZraI GACGTC 2 cut(s) 43, 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.