MD15G1170500.v1.1

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000974
Physical Location & Seq
Reverse (-)
13167330 .. 13168107
778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1170500.v1.1.491

Sequence Viewer

Length: 519 bp
ATGAATGTGAGTAATGTGGTTGTCCTTGTTCTAGCAGTGATTACCACAACATCGCTACTTGGAGCAGAAGCTGGAGAATATACAGTGGGAGATGAGTTGGGTTGGACTATTCCTCCTGGTGGCGCTGCTACGTACGAAGCATGGGCTGCCAAGCATAGATTGTCCGTTACTGATTTTATAAACTTCAACTTTAAAGTTGGAGAACAAGACTTGGCTATAGTAACCAAGGAGGATTACGACGCCTGCAACACGGACAACCCCTTATTTGTATTCCAAGAAGGCGGAGAGTTTCAGTTTGTTCAGTCGGACACATATTATTTGACCTGCACCTTCGCCGGCCACTGCACCAAAGGTCAAAAGATTGCTCTTTATATTGCTCCCGCTTTTTCTCCTTCTCCAAGTCCATCTTCATATCCAAGTGAAAAAACTGCTGCTCAACATGCTAATGCAGTCAAATTTGTGTCCAAGAAAGGTCAAGGGCTCAAGAAATTACTGCCTGTGATGATGAAGTCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

173

Amino Acids

18.58

Weight (kDa)

5.56

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 35 - 119 6.8e-13 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 332
AciI CCGC 2 cut(s) 282, 381
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 391, 423
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 3 cut(s) 125, 146, 431
ApoI RAATTY 1 cut(s) 455
AspLEI GCGC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 112, 133, 418
BccI CCATC 1 cut(s) 412
BciT130I CCWGG 1 cut(s) 117
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 126
BfuAI ACCTGC 1 cut(s) 332
BisI GCNGC 3 cut(s) 126, 147, 432
BlsI GCNGC 3 cut(s) 127, 148, 433
Bme1390I CCNGG 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 117
BpmI CTGGAG 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 225
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 335
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 119
BseXI GCAGC 3 cut(s) 112, 133, 418
BsgI GTGCAG 2 cut(s) 310, 328
BshFI GGCC 1 cut(s) 339
BsiSI CCGG 1 cut(s) 336
BsiWI CGTACG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 483
BspACI CCGC 2 cut(s) 282, 381
BspANI GGCC 1 cut(s) 339
BspMI ACCTGC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 85
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 244, 337
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstMWI GCNNNNNNNGC 2 cut(s) 146, 440
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 216
BstSNI TACGTA 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 112, 133, 418
BsuRI GGCC 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 36
BtsI GCAGTG 2 cut(s) 42, 340
BtsIMutI CAGTG 3 cut(s) 42, 90, 340
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 2 cut(s) 244, 337
CaiI CAGNNNCTG 1 cut(s) 71
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 335
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 133
CviAII CATG 2 cut(s) 141, 440
CviJI RGCY 5 cut(s) 71, 146, 215, 339, 481
CviKI_1 RGCY 5 cut(s) 71, 146, 215, 339, 481
CviQI GTAC 1 cut(s) 133
DraI TTTAAA 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 337
EciI GGCGGA 1 cut(s) 297
Eco105I TACGTA 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 2 cut(s) 144, 443
FaiI YATR 9 cut(s) 81, 142, 156, 179, 218, 313, 372, 412, 441
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FaqI GGGAC 1 cut(s) 496
FatI CATG 2 cut(s) 140, 439
FauI CCCGC 1 cut(s) 388
Fnu4HI GCNGC 3 cut(s) 126, 147, 432
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 3 cut(s) 126, 147, 432
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 126, 147, 432
GsuI CTGGAG 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 1 cut(s) 339
HapII CCGG 1 cut(s) 336
HgaI GACGC 1 cut(s) 248
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 2 cut(s) 144, 443
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HpaII CCGG 1 cut(s) 336
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 1 cut(s) 484
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 272, 340, 402
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 4 cut(s) 246, 327, 345, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 440
HpySE526I ACGT 1 cut(s) 131
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 2 cut(s) 144, 443
HspAI GCGC 1 cut(s) 123
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
LmnI GCTCC 2 cut(s) 62, 382
LpnPI CCDG 7 cut(s) 57, 102, 129, 256, 337, 349, 510
Lsp1109I GCAGC 3 cut(s) 112, 133, 418
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 166, 220
MboII GAAGA 1 cut(s) 399
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 2 cut(s) 455, 488
MmeI TCCRAC 3 cut(s) 83, 178, 285
MnlI CCTC 2 cut(s) 123, 223
MroNI GCCGGC 1 cut(s) 335
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 444
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 146, 440
NaeI GCCGGC 1 cut(s) 337
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 2 cut(s) 144, 443
NspI RCATGY 1 cut(s) 443
PdiI GCCGGC 1 cut(s) 337
Pfl23II CGTACG 1 cut(s) 132
PkrI GCNGC 3 cut(s) 127, 148, 433
Ppu21I YACGTR 1 cut(s) 132
PsiI TTATAA 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspLI CGTACG 1 cut(s) 132
PstNI CAGNNNCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 126, 147, 432
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 483
SetI ASST 6 cut(s) 73, 134, 326, 332, 355, 475
SfcI CTRYAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SnaBI TACGTA 1 cut(s) 132
Sse9I AATT 2 cut(s) 455, 488
SsiI CCGC 2 cut(s) 282, 381
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 1 cut(s) 134
TasI AATT 2 cut(s) 455, 488
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 3 cut(s) 42, 90, 347
TseI GCWGC 3 cut(s) 125, 146, 431
TspDTI ATGAA 2 cut(s) 17, 399
TspGWI ACGGA 2 cut(s) 154, 266
TspRI CASTG 3 cut(s) 42, 90, 347
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 443
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.