pycom02g02390

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000974
Physical Location & Seq
Reverse (-)
1487765 .. 1488395
631 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02390.1

Sequence Viewer

Length: 498 bp
ATGGTTAAAACCATGAATTTTGGGAATGTGATGGTTCTAGCTATAGCAGTGGTAGCAGTATCAACGATTTTACATACAACAGAAGCTGCAGAGTACGTAGTAGGAGACGACTTGGGTTGGACTGTCCCGCCAGGCGGGGCAGCCAGTTACGCTTCGTGGGCCGCAAAGTATAGATTTGTAGTTAACGATATTCTATACTTCAACTTTACAGAAGGAGAACTTACTGTGGCTTTAGTAACCAAGGAGAACTATGAGATTTGCAACACCACAGATCCCTTGTTTAAACTCGATGGACCAAGTGGACTTCAATTTCTTGCGTCAGACACATTTTACTTCACCTGCACCTTCACCGGCCATTGCGCTGGAGGCCAAAAGGTTGAGGTTTATATCGCTCCCTCTGCAAATACTCCAAGTCCAAGTCCAGGTGTTGCTCCTCCTCCTGCTGCGTCCCTGCCCTTCACATTTGTGTCCAAAAAACTTGGGTTCAGGAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

166

Amino Acids

17.65

Weight (kDa)

5.25

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 124 2.5e-13 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 347
Acc36I ACCTGC 1 cut(s) 347
AciI CCGC 3 cut(s) 128, 135, 162
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 1 cut(s) 16
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 134, 422
AgsI TTSAA 2 cut(s) 202, 308
AjnI CCWGG 2 cut(s) 130, 421
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 2 cut(s) 41, 86
AluI AGCT 2 cut(s) 41, 86
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 3 cut(s) 159, 352, 367
ApeKI GCWGC 3 cut(s) 86, 140, 443
ApoI RAATTY 1 cut(s) 16
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 2 cut(s) 159, 293
AsuHPI GGTGA 2 cut(s) 328, 340
AvaII GGWCC 1 cut(s) 293
BbvI GCAGC 3 cut(s) 73, 152, 430
BccI CCATC 2 cut(s) 25, 284
BciT130I CCWGG 2 cut(s) 132, 423
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 42, 87
BfuAI ACCTGC 1 cut(s) 347
BisI GCNGC 4 cut(s) 87, 141, 162, 444
BlsI GCNGC 4 cut(s) 88, 142, 163, 445
Bme1390I CCNGG 2 cut(s) 132, 423
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 159, 293
BmrFI CCNGG 2 cut(s) 132, 423
BpmI CTGGAG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 422
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 2 cut(s) 132, 423
BseDI CCNNGG 1 cut(s) 240
BseLI CCNNNNNNNGG 2 cut(s) 134, 422
BseMI GCAATG 1 cut(s) 355
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 2 cut(s) 423, 426
BseXI GCAGC 3 cut(s) 73, 152, 430
BsgI GTGCAG 1 cut(s) 325
BshFI GGCC 3 cut(s) 161, 354, 369
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 2 cut(s) 110, 433
BslI CCNNNNNNNGG 2 cut(s) 134, 422
BsmAI GTCTC 1 cut(s) 99
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 110, 433
BsnI GGCC 3 cut(s) 161, 354, 369
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 3 cut(s) 128, 135, 162
BspANI GGCC 3 cut(s) 161, 354, 369
BspMAI CTGCAG 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 347
BspPI GGATC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 132, 423
Bst4CI ACNGT 2 cut(s) 124, 226
BstBAI YACGTR 1 cut(s) 97
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 5 cut(s) 53, 149, 158, 366, 398
BstNI CCWGG 2 cut(s) 132, 423
BstSCI CCNGG 2 cut(s) 130, 421
BstSFI CTRYAG 2 cut(s) 42, 87
BstSNI TACGTA 1 cut(s) 97
BstV1I GCAGC 3 cut(s) 73, 152, 430
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 3 cut(s) 161, 354, 369
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 347
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 159, 293
CseI GACGC 2 cut(s) 306, 435
Csp6I GTAC 1 cut(s) 94
CviAII CATG 1 cut(s) 13
CviJI RGCY 7 cut(s) 41, 86, 143, 161, 230, 354, 369
CviKI_1 RGCY 7 cut(s) 41, 86, 143, 161, 230, 354, 369
CviQI GTAC 1 cut(s) 94
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 352
Eco105I TACGTA 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 130, 421
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 1 cut(s) 16
FaiI YATR 7 cut(s) 14, 44, 75, 171, 196, 252, 387
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 2 cut(s) 110, 433
FatI CATG 1 cut(s) 12
FauI CCCGC 2 cut(s) 128, 135
Fnu4HI GCNGC 4 cut(s) 87, 141, 162, 444
Fsp4HI GCNGC 4 cut(s) 87, 141, 162, 444
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 361
GluI GCNGC 4 cut(s) 87, 141, 162, 444
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 3 cut(s) 161, 354, 369
HapII CCGG 1 cut(s) 351
HgaI GACGC 2 cut(s) 306, 435
HhaI GCGC 1 cut(s) 362
Hin1II CATG 1 cut(s) 16
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HpaI GTTAAC 1 cut(s) 184
HpaII CCGG 1 cut(s) 351
HphI GGTGA 2 cut(s) 328, 340
Hpy166II GTNNAC 2 cut(s) 184, 302
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 2 cut(s) 184, 302
HpyAV CCTTC 3 cut(s) 206, 355, 466
HpyCH4III ACNGT 2 cut(s) 124, 226
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 4 cut(s) 89, 261, 342, 401
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 149, 158, 366, 398
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 1 cut(s) 16
HspAI GCGC 1 cut(s) 360
KspAI GTTAAC 1 cut(s) 184
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 2 cut(s) 397, 436
Lsp1109I GCAGC 3 cut(s) 73, 152, 430
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 146, 235
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MflI RGATCY 1 cut(s) 271
MluCI AATT 2 cut(s) 16, 308
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 5 cut(s) 359, 373, 406, 444, 447
MseI TTAA 3 cut(s) 6, 183, 282
MslI CAYNNNNRTG 1 cut(s) 464
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 2 cut(s) 132, 423
MssI GTTTAAAC 1 cut(s) 283
MvaI CCWGG 2 cut(s) 132, 423
MwoI GCNNNNNNNGC 5 cut(s) 53, 149, 158, 366, 398
NdeII GATC 1 cut(s) 271
NlaIII CATG 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 464
PaqCI CACCTGC 1 cut(s) 347
PkrI GCNGC 4 cut(s) 88, 142, 163, 445
PmeI GTTTAAAC 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 130, 421
PspGI CCWGG 2 cut(s) 130, 421
PspPI GGNCC 2 cut(s) 159, 293
PstI CTGCAG 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 3 cut(s) 6, 183, 282
SatI GCNGC 4 cut(s) 87, 141, 162, 444
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 2 cut(s) 159, 293
ScrFI CCNGG 2 cut(s) 132, 423
SetI ASST 8 cut(s) 43, 88, 99, 341, 347, 378, 384, 427
SfcI CTRYAG 2 cut(s) 42, 87
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 464
SnaBI TACGTA 1 cut(s) 97
Sse9I AATT 2 cut(s) 16, 308
SsiI CCGC 3 cut(s) 128, 135, 162
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 130, 421
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 2 cut(s) 124, 226
TaiI ACGT 1 cut(s) 99
TaqI TCGA 1 cut(s) 288
TasI AATT 2 cut(s) 16, 308
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 3 cut(s) 6, 183, 282
Tru9I TTAA 3 cut(s) 6, 183, 282
TscAI CASTG 1 cut(s) 54
TseI GCWGC 3 cut(s) 86, 140, 443
TspDTI ATGAA 1 cut(s) 29
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 16
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.