Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001491

Physical Location & Seq

Genomic Coordinates Reverse (-)

4758133 .. 4759968

Gene Structure 1836 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1067700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 822 bp

ATGGCCTGGCCTAAATCAAAGCAGGCCCAAACGGCCCAAACCGTAAAATCTGCACAAACGAAGGATTCCAGATTCTCTGACTCACCTCCGAAGAGAACGAAGAACACCCCCCTCCCCCTTTCCTCTCGGTTTCTCTCCGAGTCGCCGCATTTTTCCCACCCGAGTCATCCCTCAGTCAGAGCAATGGTCGGATTGCGCCTTCTGCTTGCTGCCGTAATCGCAGGGTTCTTATTATCTACATCCGAATCTCTGCGGTTCGAGCTGCAATCCGGTCACACGAAATGCATCGTCGAAGACATCAAAACCAACGCCATGACCGTCGGAAAATACAGCGTCGTCAACCCCAACGAGGGTCAGCCCATGCCCGATTCCCACAAATTGACCGTCCGGGTGACGTCTCACCACGGGAACAACTATCACTACTCGCAGCTGGTGGAATCGGGGCAGTATGCGTTTGTGGCGGCGGAGGAGGGAGATTACATGGCTTGCTTCTGGGCGCCGGATCACAAGCCCCAGACGACGTTGACCATTGAATTCGATTGGAAAACCGGCGTCGCCGCCAAAGACTGGTCCAATGTCGCTAAGAAAGGCACCGTCGACGGAATGGAATTAGAGCTTAGGAAGTTGCTCGATACGGTTACGTCCATTCACGAGGAGATGTCCTATCTCCGTGGAAGGGAAGAAGAGATGCAGGACCTCAACAGAACAACGAACTCGAGGATGGCCACGTTGAGTCTTCTTTCGCTTTTTGTATGCTTAGCAGTCGCAGGTATGCAGTTGTGGCACCTCAAGACCTTCTTCGAGAAGAAGAAGCTTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005793 GO:0005794 GO:0005798 GO:0005801 GO:0005886 GO:0006810 GO:0006886 GO:0006887 GO:0006890 GO:0006900 GO:0006903 GO:0006996 GO:0007030 GO:0008104 GO:0008150 GO:0009987 GO:0010256 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0019222 GO:0019905 GO:0022607 GO:0030133 GO:0030135 GO:0030136 GO:0030137 GO:0030140 GO:0030141 GO:0030659 GO:0030667 GO:0031090 GO:0031224 GO:0031323 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0033036 GO:0034248 GO:0034613 GO:0035964 GO:0042588 GO:0042589 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0044877 GO:0045055 GO:0045184 GO:0046903 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0050789 GO:0050794 GO:0051171 GO:0051179 GO:0051234 GO:0051246 GO:0051259 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0060255 GO:0061024 GO:0065003 GO:0065007 GO:0070727 GO:0070765 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098797 GO:0098805 GO:0099503 GO:1902003 GO:1902991

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP24_GP25L

Protein Analysis

274

Amino Acids

30.64

Weight (kDa)

8.86

Isoelectric Point (pI)

47.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP24_GP25L	PF01105	84 - 268	2.7e-50	emp24/gp25L/p24 family/GOLD

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14010 AT1G26690 AT2G03040 AT2G03290
fragaria_vesca	FvH4_4g30790 FvH4_6g48050
malus_domestica	MD00G1214100.v1.1 MD09G1054500.v1.1 MD15G1422900.v1.1 MD16G1067700.v1.1 MD17G1052400.v1.1
prunus_persica	Prupe.1G279100_v2.0.a1 Prupe.3G264000_v2.0.a1
pyrus_communis	pycom111g04580 pycom13g06060 pycom13g06070 pycom16g05910 pycom16g05920 pycom17g04930
rosa_chinensis	RchiOBHm_Chr1g0316241 RchiOBHm_Chr1g0316281 RchiOBHm_Chr2g0167341 RchiOBHm_Chr4g0439181
rosa_laevigata	RLG00000006290 RLG00000021710 RLG00000021729 RLG00000022059 RLG00000023281
rosa_multiflora	Rmu_sc0001840.1_g000010 Rmu_sc0002236.1_g000009 Rmu_sc0002531.1_g000018 Rmu_sc0007793.1_g000012 Rmu_sc0018330.1_g000001
rosa_roxburghii	Rroxscaffold_2G00083580 Rroxscaffold_5G00380170
rosa_rugosa	Rorug02G0529700 Rorug04G0316400
rosa_samantha	Rh1DG014200 Rh2AG596700 Rh2BG608100 Rh2CG578900 Rh2DG619500 Rh4AG367300 Rh4BG378900 Rh4CG393300 Rh4DG373500
rosa_wichuraiana	Rw0G011670 Rw1G001280 Rw2G049660 Rw3G022980 Rw5G028510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	398
Acc36I	ACCTGC	1 cut(s)	758
AccB1I	GGYRCC	3 cut(s)	496, 590, 783
AccB7I	CCANNNNNTGG	1 cut(s)	567
AccI	GTMKAC	1 cut(s)	597
AciI	CCGC	5 cut(s)	146, 253, 461, 464, 558
AclWI	GGATC	1 cut(s)	510
AcoI	YGGCCR	1 cut(s)	723
AcsI	RAATTY	1 cut(s)	533
AcyI	GRCGYC	3 cut(s)	395, 497, 552
AfiI	CCNNNNNNNGG	4 cut(s)	349, 350, 567, 676
AgsI	TTSAA	1 cut(s)	533
AjnI	CCWGG	1 cut(s)	5
AluBI	AGCT	4 cut(s)	262, 430, 616, 814
AluI	AGCT	4 cut(s)	262, 430, 616, 814
Alw26I	GTCTC	1 cut(s)	402
AlwI	GGATC	1 cut(s)	510
Ama87I	CYCGRG	2 cut(s)	160, 715
AoxI	GGCC	5 cut(s)	3, 8, 24, 33, 723
ApeKI	GCWGC	3 cut(s)	209, 262, 427
ApoI	RAATTY	1 cut(s)	533
ArsI	GACNNNNNNTTYG	2 cut(s)	369, 401
AspLEI	GCGC	2 cut(s)	198, 499
AspS9I	GGNCC	4 cut(s)	25, 34, 570, 694
AsuC2I	CCSGG	1 cut(s)	389
AsuHPI	GGTGA	3 cut(s)	75, 392, 403
AvaI	CYCGRG	2 cut(s)	160, 715
AvaII	GGWCC	2 cut(s)	570, 694
BalI	TGGCCA	1 cut(s)	725
BanI	GGYRCC	3 cut(s)	496, 590, 783
BauI	CACGAG	1 cut(s)	650
BbsI	GAAGAC	2 cut(s)	300, 728
BbvI	GCAGC	3 cut(s)	196, 249, 439
BccI	CCATC	1 cut(s)	715
BceAI	ACGGC	2 cut(s)	48, 197
BciT130I	CCWGG	1 cut(s)	7
BcnI	CCSGG	1 cut(s)	389
BcoDI	GTCTC	1 cut(s)	402
BfoI	RGCGCY	1 cut(s)	500
BfuAI	ACCTGC	1 cut(s)	758
BglI	GCCNNNNNGGC	1 cut(s)	32
BisI	GCNGC	6 cut(s)	146, 210, 263, 428, 462, 558
BlpI	GCTNAGC	1 cut(s)	757
BlsI	GCNGC	6 cut(s)	147, 211, 264, 429, 463, 559
Bme1390I	CCNGG	2 cut(s)	7, 389
Bme18I	GGWCC	2 cut(s)	570, 694
BmeT110I	CYCGRG	2 cut(s)	160, 715
BmgT120I	GGNCC	4 cut(s)	25, 34, 570, 694
BmiI	GGNNCC	3 cut(s)	498, 592, 785
BmrFI	CCNGG	2 cut(s)	7, 389
BmsI	GCATC	2 cut(s)	294, 678
BpiI	GAAGAC	2 cut(s)	300, 728
Bpu10I	CCTNAGC	1 cut(s)	617
Bpu1102I	GCTNAGC	1 cut(s)	757
BpuEI	CTTGAG	1 cut(s)	773
BpuMI	CCSGG	1 cut(s)	389
BsaHI	GRCGYC	3 cut(s)	395, 497, 552
BsaJI	CCNNGG	2 cut(s)	403, 670
BsaWI	WCCGGW	1 cut(s)	269
Bsc4I	CCNNNNNNNGG	4 cut(s)	349, 350, 567, 676
Bse118I	RCCGGY	1 cut(s)	548
Bse1I	ACTGG	1 cut(s)	572
Bse3DI	GCAATG	1 cut(s)	189
BseBI	CCWGG	1 cut(s)	7
BseDI	CCNNGG	2 cut(s)	403, 670
BseGI	GGATG	3 cut(s)	166, 239, 726
BseLI	CCNNNNNNNGG	4 cut(s)	349, 350, 567, 676
BseMI	GCAATG	1 cut(s)	189
BseMII	CTCAG	1 cut(s)	186
BseNI	ACTGG	1 cut(s)	572
BseRI	GAGGAG	2 cut(s)	482, 668
BseXI	GCAGC	3 cut(s)	196, 249, 439
BsgI	GTGCAG	1 cut(s)	36
BshFI	GGCC	5 cut(s)	5, 10, 26, 35, 725
BshNI	GGYRCC	3 cut(s)	496, 590, 783
BsiHKCI	CYCGRG	2 cut(s)	160, 715
BsiSI	CCGG	4 cut(s)	270, 388, 500, 549
BslI	CCNNNNNNNGG	4 cut(s)	349, 350, 567, 676
BsmAI	GTCTC	1 cut(s)	402
BsmBI	CGTCTC	1 cut(s)	402
BsnI	GGCC	5 cut(s)	5, 10, 26, 35, 725
BsoBI	CYCGRG	2 cut(s)	160, 715
Bsp143I	GATC	1 cut(s)	502
Bsp1720I	GCTNAGC	1 cut(s)	757
BspACI	CCGC	5 cut(s)	146, 253, 461, 464, 558
BspANI	GGCC	5 cut(s)	5, 10, 26, 35, 725
BspCNI	CTCAG	1 cut(s)	185
BspLI	GGNNCC	3 cut(s)	498, 592, 785
BspMI	ACCTGC	1 cut(s)	758
BspPI	GGATC	1 cut(s)	510
BspT107I	GGYRCC	3 cut(s)	496, 590, 783
BsrDI	GCAATG	1 cut(s)	189
BsrFI	RCCGGY	1 cut(s)	548
BsrI	ACTGG	1 cut(s)	572
BssAI	RCCGGY	1 cut(s)	548
BssECI	CCNNGG	2 cut(s)	403, 670
BssMI	GATC	1 cut(s)	502
BssNI	GRCGYC	3 cut(s)	395, 497, 552
BssSI	CACGAG	1 cut(s)	650
Bst2BI	CACGAG	1 cut(s)	650
Bst2UI	CCWGG	1 cut(s)	7
Bst4CI	ACNGT	5 cut(s)	43, 319, 385, 595, 637
Bst6I	CTCTTC	2 cut(s)	86, 678
BstACI	GRCGYC	3 cut(s)	395, 497, 552
BstC8I	GCNNGC	3 cut(s)	24, 207, 487
BstDEI	CTNAG	4 cut(s)	172, 582, 617, 757
BstDSI	CCRYGG	2 cut(s)	403, 670
BstF5I	GGATG	3 cut(s)	166, 239, 726
BstH2I	RGCGCY	1 cut(s)	500
BstHHI	GCGC	2 cut(s)	198, 499
BstKTI	GATC	1 cut(s)	505
BstMAI	GTCTC	1 cut(s)	402
BstMBI	GATC	1 cut(s)	502
BstMWI	GCNNNNNNNGC	6 cut(s)	32, 202, 218, 259, 458, 781
BstNI	CCWGG	1 cut(s)	7
BstSCI	CCNGG	2 cut(s)	5, 387
BstV1I	GCAGC	3 cut(s)	196, 249, 439
BstV2I	GAAGAC	2 cut(s)	300, 728
BsuRI	GGCC	5 cut(s)	5, 10, 26, 35, 725
BtgI	CCRYGG	2 cut(s)	403, 670
BtsCI	GGATG	3 cut(s)	166, 239, 726
BveI	ACCTGC	1 cut(s)	758
Cac8I	GCNNGC	3 cut(s)	24, 207, 487
CfoI	GCGC	2 cut(s)	198, 499
Cfr10I	RCCGGY	1 cut(s)	548
Cfr13I	GGNCC	4 cut(s)	25, 34, 570, 694
CseI	GACGC	2 cut(s)	322, 541
CviAII	CATG	3 cut(s)	313, 361, 481
DdeI	CTNAG	4 cut(s)	172, 582, 617, 757
DinI	GGCGCC	1 cut(s)	498
DpnI	GATC	1 cut(s)	504
DpnII	GATC	1 cut(s)	502
EaeI	YGGCCR	1 cut(s)	723
Eam1104I	CTCTTC	2 cut(s)	86, 678
EarI	CTCTTC	2 cut(s)	86, 678
EciI	GGCGGA	1 cut(s)	479
Eco47I	GGWCC	2 cut(s)	570, 694
Eco88I	CYCGRG	2 cut(s)	160, 715
EcoO109I	RGGNCCY	1 cut(s)	694
EcoRI	GAATTC	1 cut(s)	533
EcoRII	CCWGG	1 cut(s)	5
EcoT22I	ATGCAT	1 cut(s)	287
EgeI	GGCGCC	1 cut(s)	498
EheI	GGCGCC	1 cut(s)	498
Esp3I	CGTCTC	1 cut(s)	402
FaeI	CATG	3 cut(s)	316, 364, 484
FaiI	YATR	6 cut(s)	314, 362, 450, 482, 754, 773
FalI	AAGNNNNNCTT	2 cut(s)	782, 814
FatI	CATG	3 cut(s)	312, 360, 480
FblI	GTMKAC	1 cut(s)	597
Fnu4HI	GCNGC	6 cut(s)	146, 210, 263, 428, 462, 558
FokI	GGATG	3 cut(s)	153, 226, 733
Fsp4HI	GCNGC	6 cut(s)	146, 210, 263, 428, 462, 558
GlaI	GCGC	2 cut(s)	197, 498
GluI	GCNGC	6 cut(s)	146, 210, 263, 428, 462, 558
HaeII	RGCGCY	1 cut(s)	500
HaeIII	GGCC	5 cut(s)	5, 10, 26, 35, 725
HapII	CCGG	4 cut(s)	270, 388, 500, 549
HgaI	GACGC	2 cut(s)	322, 541
HhaI	GCGC	2 cut(s)	198, 499
Hin1I	GRCGYC	3 cut(s)	395, 497, 552
Hin1II	CATG	3 cut(s)	316, 364, 484
Hin6I	GCGC	2 cut(s)	196, 497
HinP1I	GCGC	2 cut(s)	196, 497
HincII	GTYRAC	3 cut(s)	340, 525, 598
HindII	GTYRAC	3 cut(s)	340, 525, 598
HindIII	AAGCTT	1 cut(s)	812
HinfI	GANTC	9 cut(s)	65, 72, 80, 140, 163, 245, 368, 437, 733
HpaII	CCGG	4 cut(s)	270, 388, 500, 549
HphI	GGTGA	3 cut(s)	75, 392, 403
Hpy166II	GTNNAC	3 cut(s)	340, 525, 598
Hpy188I	TCNGA	7 cut(s)	79, 90, 139, 179, 191, 244, 323
Hpy188III	TCNNGA	4 cut(s)	69, 650, 790, 802
Hpy8I	GTNNAC	3 cut(s)	340, 525, 598
Hpy99I	CGWCG	7 cut(s)	293, 323, 338, 523, 557, 599, 602
HpyAV	CCTTC	4 cut(s)	55, 209, 669, 805
HpyCH4III	ACNGT	5 cut(s)	43, 319, 385, 595, 637
HpyCH4IV	ACGT	4 cut(s)	395, 521, 641, 728
HpyCH4V	TGCA	5 cut(s)	53, 265, 285, 691, 775
HpyF10VI	GCNNNNNNNGC	6 cut(s)	32, 202, 218, 259, 458, 781
HpyF3I	CTNAG	4 cut(s)	172, 582, 617, 757
HpySE526I	ACGT	4 cut(s)	395, 521, 641, 728
Hsp92I	GRCGYC	3 cut(s)	395, 497, 552
Hsp92II	CATG	3 cut(s)	316, 364, 484
HspAI	GCGC	2 cut(s)	196, 497
KasI	GGCGCC	1 cut(s)	496
Kzo9I	GATC	1 cut(s)	502
Lsp1109I	GCAGC	3 cut(s)	196, 249, 439
LweI	GCATC	2 cut(s)	294, 678
MaeII	ACGT	4 cut(s)	395, 521, 641, 728
MaeIII	GTNAC	3 cut(s)	272, 391, 637
MalI	GATC	1 cut(s)	504
MboI	GATC	1 cut(s)	502
MboII	GAAGA	9 cut(s)	103, 112, 305, 692, 695, 728, 790, 817, 820
MlsI	TGGCCA	1 cut(s)	725
MluCI	AATT	3 cut(s)	377, 533, 608
MluNI	TGGCCA	1 cut(s)	725
Mly113I	GGCGCC	1 cut(s)	497
MlyI	GAGTC	4 cut(s)	74, 149, 172, 742
MmeI	TCCRAC	2 cut(s)	169, 301
Mox20I	TGGCCA	1 cut(s)	725
Mph1103I	ATGCAT	1 cut(s)	287
MscI	TGGCCA	1 cut(s)	725
Msp20I	TGGCCA	1 cut(s)	725
MspA1I	CMGCKG	1 cut(s)	430
MspI	CCGG	4 cut(s)	270, 388, 500, 549
MspR9I	CCNGG	2 cut(s)	7, 389
MvaI	CCWGG	1 cut(s)	7
MwoI	GCNNNNNNNGC	6 cut(s)	32, 202, 218, 259, 458, 781
NarI	GGCGCC	1 cut(s)	497
NciI	CCSGG	1 cut(s)	389
NdeII	GATC	1 cut(s)	502
NlaIII	CATG	3 cut(s)	316, 364, 484
NlaIV	GGNNCC	3 cut(s)	498, 592, 785
NmuCI	GTSAC	2 cut(s)	272, 391
NsiI	ATGCAT	1 cut(s)	287
PaeR7I	CTCGAG	1 cut(s)	715
PcsI	WCGNNNNNNNCGW	1 cut(s)	315
PfeI	GAWTC	5 cut(s)	65, 72, 245, 368, 437
PflMI	CCANNNNNTGG	1 cut(s)	567
PkrI	GCNGC	6 cut(s)	147, 211, 264, 429, 463, 559
PleI	GAGTC	4 cut(s)	74, 148, 171, 741
PluTI	GGCGCC	1 cut(s)	500
PpsI	GAGTC	4 cut(s)	74, 148, 171, 741
PpuMI	RGGWCCY	1 cut(s)	694
Psp5II	RGGWCCY	1 cut(s)	694
Psp6I	CCWGG	1 cut(s)	5
PspGI	CCWGG	1 cut(s)	5
PspN4I	GGNNCC	3 cut(s)	498, 592, 785
PspPI	GGNCC	4 cut(s)	25, 34, 570, 694
PspPPI	RGGWCCY	1 cut(s)	694
PspXI	VCTCGAGB	1 cut(s)	715
PvuII	CAGCTG	1 cut(s)	430
SalI	GTCGAC	1 cut(s)	596
SatI	GCNGC	6 cut(s)	146, 210, 263, 428, 462, 558
Sau3AI	GATC	1 cut(s)	502
Sau96I	GGNCC	4 cut(s)	25, 34, 570, 694
SchI	GAGTC	4 cut(s)	74, 149, 172, 742
ScrFI	CCNGG	2 cut(s)	7, 389
SfaNI	GCATC	2 cut(s)	294, 678
SfiI	GGCCNNNNNGGCC	1 cut(s)	32
SfoI	GGCGCC	1 cut(s)	498
Sfr274I	CTCGAG	1 cut(s)	715
SgrDI	CGTCGACG	1 cut(s)	596
SinI	GGWCC	2 cut(s)	570, 694
SlaI	CTCGAG	1 cut(s)	715
SmlI	CTYRAG	2 cut(s)	715, 788
SmoI	CTYRAG	2 cut(s)	715, 788
Sse9I	AATT	3 cut(s)	377, 533, 608
SsiI	CCGC	5 cut(s)	146, 253, 461, 464, 558
SspDI	GGCGCC	1 cut(s)	496
StyD4I	CCNGG	2 cut(s)	5, 387
TaaI	ACNGT	5 cut(s)	43, 319, 385, 595, 637
TaiI	ACGT	4 cut(s)	398, 524, 644, 731
TaqI	TCGA	7 cut(s)	258, 291, 537, 597, 630, 716, 801
TasI	AATT	3 cut(s)	377, 533, 608
TauI	GCSGC	3 cut(s)	148, 464, 560
TfiI	GAWTC	5 cut(s)	65, 72, 245, 368, 437
TseFI	GTSAC	2 cut(s)	272, 391
TseI	GCWGC	3 cut(s)	209, 262, 427
Tsp45I	GTSAC	2 cut(s)	272, 391
TspGWI	ACGGA	2 cut(s)	615, 659
Van91I	CCANNNNNTGG	1 cut(s)	567
VpaK11BI	GGWCC	2 cut(s)	570, 694
XapI	RAATTY	1 cut(s)	533
XhoI	CTCGAG	1 cut(s)	715
XmiI	GTMKAC	1 cut(s)	597
ZraI	GACGTC	1 cut(s)	396
Zsp2I	ATGCAT	1 cut(s)	287

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.