Rorug02G0529700

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001491
Physical Location & Seq
Forward (+)
65911604 .. 65913123
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0529700.1

Sequence Viewer

Length: 909 bp
ATGGGGCTTCCGGGGATTTGGGTGGCGCTTCTGATGACGCCTCGCGCACGTTTCAGGGGAAGGAAATGGAATTTACTTGACCTAGTCACAGCTGCAGTGTTTGCGTCTATGCATGTTCTTGCTTTATTTGGGCCATTTTATTTCAATTGGTCTGCGTTATGGGTAGCACTCGCGCTCGTTTACGTAACCGGATTGGGAATCACTCTTTCATATCACAGAAACCTTGCGCACAGGAGCTTCAGGCTTCCCAAGTTGCTCGAGTACTTGTTTGCCTATTTTGCAGTTCTTTCAGCTCAGGGTAGTCCCATTGATTGGGTGAGTACACACCGTTACCACCACCAGTATACAGATACAGAGAAAGATGCTCATAGCCCACTAAAAGGTAGAAGGTTTAGTCACATGGGTTGGATTCTTGATAGCAACGCTCGTTTTGGAAGATATGGAGGACTGCGGAATGTTGAAGATTTGAAAAGGCAAGCATTCTATAGGTTTCTTCATAATACTTACCTTTTACACTCAATTATTCTTGGAGGCATTTTATATGCAGTTGGTGGATTTCCGTTCATCGTTTGGGGAATGGGCGTGAGGATGGTATATGTTTTCCACGGCACATTGCTAGTGAATTCGGCCGGCCACATATGGGGATATCAAGCATGGAAGACCAGTGATCTGTCTAAGAATCTCTGGTGGTTGGCATTGGTTGCATTTGGAGAAGGGTGGCACAACAACCACCATGCTTTCGAATATTCGGCTCGACAGGGCCTCGAATGGTGGCAGTTTGACCTGACTTGGTACATCATAAAATTTCTTGAAGCTCTAGGGTTGGCAACTGATGTGAAAGTACCATCTGAGGCTCATAAGAAACGTAAAGCTTTAGAGACCAAAACAACCATGGCTGCCATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

302

Amino Acids

34.84

Weight (kDa)

9.9

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 49 - 267 1.3e-18 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 228
AccB7I CCANNNNNTGG 1 cut(s) 312
AccI GTMKAC 1 cut(s) 344
AccII CGCG 2 cut(s) 45, 173
AciI CCGC 1 cut(s) 451
AcoI YGGCCR 2 cut(s) 627, 631
AcsI RAATTY 3 cut(s) 70, 622, 803
AcuI CTGAAG 1 cut(s) 223
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 4 cut(s) 263, 322, 794, 843
AfiI CCNNNNNNNGG 3 cut(s) 312, 380, 640
AgsI TTSAA 4 cut(s) 145, 461, 469, 812
AluBI AGCT 5 cut(s) 92, 237, 293, 815, 872
AluI AGCT 5 cut(s) 92, 237, 293, 815, 872
Alw26I GTCTC 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 4 cut(s) 131, 627, 631, 760
ApeKI GCWGC 2 cut(s) 92, 896
ApoI RAATTY 3 cut(s) 70, 622, 803
AspLEI GCGC 4 cut(s) 28, 47, 175, 229
AspS9I GGNCC 2 cut(s) 131, 760
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 741
AvaI CYCGRG 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 665
BbvI GCAGC 2 cut(s) 79, 883
BccI CCATC 2 cut(s) 583, 853
BceAI ACGGC 1 cut(s) 622
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 872
BfaI CTAG 3 cut(s) 83, 617, 818
BfmI CTRYAG 2 cut(s) 93, 484
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 2 cut(s) 93, 897
BlsI GCNGC 2 cut(s) 94, 898
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 131, 760
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 665
Bpu10I CCTNAGC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 12
BsaAI YACGTR 1 cut(s) 184
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 3 cut(s) 11, 604, 891
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 380, 640
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 2 cut(s) 340, 663
Bse3DI GCAATG 1 cut(s) 611
BseDI CCNNGG 3 cut(s) 11, 604, 891
BseGI GGATG 1 cut(s) 594
BseLI CCNNNNNNNGG 3 cut(s) 312, 380, 640
BseMI GCAATG 1 cut(s) 611
BseMII CTCAG 2 cut(s) 308, 840
BseNI ACTGG 2 cut(s) 340, 663
BseX3I CGGCCG 1 cut(s) 627
BseXI GCAGC 2 cut(s) 79, 883
Bsh1236I CGCG 2 cut(s) 45, 173
Bsh1285I CGRYCG 1 cut(s) 630
BshFI GGCC 4 cut(s) 133, 629, 633, 762
BsiEI CGRYCG 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 11, 189, 630
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 3 cut(s) 312, 380, 640
BsmAI GTCTC 1 cut(s) 872
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 4 cut(s) 133, 629, 633, 762
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 741
Bsp143I GATC 1 cut(s) 667
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 1 cut(s) 451
BspANI GGCC 4 cut(s) 133, 629, 633, 762
BspCNI CTCAG 2 cut(s) 307, 841
BspFNI CGCG 2 cut(s) 45, 173
BspMAI CTGCAG 1 cut(s) 97
BspT104I TTCGAA 1 cut(s) 741
BspTNI GGTCTC 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 611
BsrFI RCCGGY 1 cut(s) 629
BsrI ACTGG 2 cut(s) 340, 663
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 3 cut(s) 11, 604, 891
BssMI GATC 1 cut(s) 667
BssNAI GTATAC 1 cut(s) 345
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 891
Bst1107I GTATAC 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 329
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 2 cut(s) 101, 701
BstBAI YACGTR 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 741
BstC8I GCNNGC 2 cut(s) 477, 631
BstDEI CTNAG 3 cut(s) 294, 675, 849
BstDSI CCRYGG 2 cut(s) 604, 891
BstF5I GGATG 1 cut(s) 594
BstFNI CGCG 2 cut(s) 45, 173
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 4 cut(s) 28, 47, 175, 229
BstKTI GATC 1 cut(s) 670
BstMAI GTCTC 1 cut(s) 872
BstMBI GATC 1 cut(s) 667
BstMCI CGRYCG 1 cut(s) 630
BstMWI GCNNNNNNNGC 3 cut(s) 101, 278, 701
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 2 cut(s) 93, 484
BstSNI TACGTA 1 cut(s) 184
BstUI CGCG 2 cut(s) 45, 173
BstV1I GCAGC 2 cut(s) 79, 883
BstV2I GAAGAC 1 cut(s) 665
BstZ17I GTATAC 1 cut(s) 345
BstZI CGGCCG 1 cut(s) 627
BsuRI GGCC 4 cut(s) 133, 629, 633, 762
BtgI CCRYGG 2 cut(s) 604, 891
BtsCI GGATG 1 cut(s) 594
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 102, 670
Cac8I GCNNGC 2 cut(s) 477, 631
CfoI GCGC 4 cut(s) 28, 47, 175, 229
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 2 cut(s) 131, 760
CseI GACGC 2 cut(s) 46, 93
Csp6I GTAC 4 cut(s) 262, 321, 793, 842
CviAII CATG 6 cut(s) 113, 400, 654, 734, 892, 901
CviQI GTAC 4 cut(s) 262, 321, 793, 842
DdeI CTNAG 3 cut(s) 294, 675, 849
DpnI GATC 1 cut(s) 669
DpnII GATC 1 cut(s) 667
EaeI YGGCCR 2 cut(s) 627, 631
EagI CGGCCG 1 cut(s) 627
EclXI CGGCCG 1 cut(s) 627
Eco105I TACGTA 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 891
Eco31I GGTCTC 1 cut(s) 872
Eco32I GATATC 1 cut(s) 647
Eco52I CGGCCG 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 622
EcoRV GATATC 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 6 cut(s) 116, 403, 657, 737, 895, 904
FaqI GGGAC 1 cut(s) 288
FatI CATG 6 cut(s) 112, 399, 653, 733, 891, 900
FauNDI CATATG 1 cut(s) 638
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 2 cut(s) 93, 897
FokI GGATG 1 cut(s) 601
FseI GGCCGGCC 1 cut(s) 633
Fsp4HI GCNGC 2 cut(s) 93, 897
FspBI CTAG 3 cut(s) 83, 617, 818
FspI TGCGCA 1 cut(s) 228
GlaI GCGC 4 cut(s) 27, 46, 174, 228
GluI GCNGC 2 cut(s) 93, 897
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 4 cut(s) 133, 629, 633, 762
HapII CCGG 3 cut(s) 11, 189, 630
HgaI GACGC 2 cut(s) 46, 93
HhaI GCGC 4 cut(s) 28, 47, 175, 229
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 6 cut(s) 116, 403, 657, 737, 895, 904
Hin6I GCGC 4 cut(s) 26, 45, 173, 227
HinP1I GCGC 4 cut(s) 26, 45, 173, 227
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 3 cut(s) 198, 409, 679
HpaII CCGG 3 cut(s) 11, 189, 630
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 3 cut(s) 181, 323, 345
Hpy188I TCNGA 2 cut(s) 33, 850
Hpy188III TCNNGA 2 cut(s) 413, 809
Hpy8I GTNNAC 3 cut(s) 181, 323, 345
HpyAV CCTTC 3 cut(s) 54, 381, 707
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 3 cut(s) 49, 183, 865
HpyCH4V TGCA 5 cut(s) 95, 112, 281, 545, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 278, 701
HpyF3I CTNAG 3 cut(s) 294, 675, 849
HpySE526I ACGT 3 cut(s) 49, 183, 865
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 6 cut(s) 116, 403, 657, 737, 895, 904
HspAI GCGC 4 cut(s) 26, 45, 173, 227
KroI GCCGGC 1 cut(s) 629
KroNI GCCGGC 1 cut(s) 631
Kzo9I GATC 1 cut(s) 667
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 2 cut(s) 79, 883
LweI GCATC 1 cut(s) 352
MaeI CTAG 3 cut(s) 83, 617, 818
MaeII ACGT 3 cut(s) 49, 183, 865
MaeIII GTNAC 4 cut(s) 85, 184, 329, 395
MalI GATC 1 cut(s) 669
MboI GATC 1 cut(s) 667
MboII GAAGA 4 cut(s) 447, 473, 485, 670
MfeI CAATTG 1 cut(s) 145
MluCI AATT 5 cut(s) 70, 145, 519, 622, 803
MmeI TCCRAC 1 cut(s) 386
MnlI CCTC 6 cut(s) 51, 437, 524, 579, 773, 844
Mph1103I ATGCAT 1 cut(s) 114
MroNI GCCGGC 1 cut(s) 629
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 3 cut(s) 11, 189, 630
MspR9I CCNGG 1 cut(s) 12
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 479
MvnI CGCG 2 cut(s) 45, 173
MwoI GCNNNNNNNGC 3 cut(s) 101, 278, 701
NaeI GCCGGC 1 cut(s) 631
NciI CCSGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 891
NdeI CATATG 1 cut(s) 638
NdeII GATC 1 cut(s) 667
NgoMIV GCCGGC 1 cut(s) 629
NlaIII CATG 6 cut(s) 116, 403, 657, 737, 895, 904
NmuCI GTSAC 2 cut(s) 85, 395
NsbI TGCGCA 1 cut(s) 228
NsiI ATGCAT 1 cut(s) 114
NspI RCATGY 1 cut(s) 116
NspV TTCGAA 1 cut(s) 741
PaeR7I CTCGAG 1 cut(s) 257
PctI GAATGC 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 631
PfeI GAWTC 3 cut(s) 198, 409, 679
PflFI GACNNNGTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 312
PkrI GCNGC 2 cut(s) 94, 898
Ppu21I YACGTR 1 cut(s) 184
PspPI GGNCC 2 cut(s) 131, 760
PspXI VCTCGAGB 1 cut(s) 257
PstI CTGCAG 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 92
RigI GGCCGGCC 1 cut(s) 633
RsaI GTAC 4 cut(s) 263, 322, 794, 843
RsaNI GTAC 4 cut(s) 262, 321, 793, 842
SatI GCNGC 2 cut(s) 93, 897
Sau3AI GATC 1 cut(s) 667
Sau96I GGNCC 2 cut(s) 131, 760
ScaI AGTACT 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 12
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 2 cut(s) 93, 484
Sfr274I CTCGAG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 741
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SnaBI TACGTA 1 cut(s) 184
Sse9I AATT 5 cut(s) 70, 145, 519, 622, 803
SsiI CCGC 1 cut(s) 451
SspI AATATT 1 cut(s) 746
SspMI CTAG 3 cut(s) 83, 617, 818
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 3 cut(s) 52, 186, 868
TaqI TCGA 4 cut(s) 258, 741, 754, 765
TasI AATT 5 cut(s) 70, 145, 519, 622, 803
TatI WGTACW 2 cut(s) 261, 320
TfiI GAWTC 3 cut(s) 198, 409, 679
TscAI CASTG 2 cut(s) 102, 670
TseFI GTSAC 2 cut(s) 85, 395
TseI GCWGC 2 cut(s) 92, 896
Tsp45I GTSAC 2 cut(s) 85, 395
TspDTI ATGAA 3 cut(s) 198, 485, 553
TspGWI ACGGA 1 cut(s) 549
TspRI CASTG 2 cut(s) 102, 670
Tth111I GACNNNGTC 1 cut(s) 83
Van91I CCANNNNNTGG 1 cut(s) 312
XapI RAATTY 3 cut(s) 70, 622, 803
XceI RCATGY 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 344
XspI CTAG 3 cut(s) 83, 617, 818
ZrmI AGTACT 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.