Rroxscaffold_2G00083580

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001491
Physical Location & Seq
Forward (+)
6270757 .. 6273072
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083580.1

Sequence Viewer

Length: 804 bp
ATGTTTAACAAACACAAACAGATCTCCTCCGTTTGGAGTCTCTCTCTCTCTCCGAGAAGCTCATACTCCGATCAACACTGTCTCCGAACGCCTCGGTCTCCAAATTGGTCTCCCCACCAACTCAAAACCCTCATTTTTTTCTCATTGGAACCATCACTTTCTGACACAATGTCGCCAATATTGATCTGTACATTTCTGATATTGTGCTTAATGTCAACGATGGCGGTTTCGATGCGGTTCGACCTTCAATCTGGCGCCACTAAATGCATCTCAGACGAAATAAAGAGCAATTCCATGACTGTGGGCAAGTACTCCACCGTCAATCCCAATGAGGGCTTTCCTCTTCCTGATACGCACAAAGTCACTGTCAGGGTGACCTCACCTCATGGCAATAATTATCACCATGGGGATCTTGTGGATACGGGTAATTTTGCCTTCACTGCGGCGGAGACTGGTGATTATTCAGTATGCTTTTGGGTAGCTGATCATAAGCCTCCGACCACGGTGACGATTGATTTTGACTGGAAAACTGGAGTTGAGGCCAAGGACTGGTCCAAGGTTGCCAAGAAAGGGCAGATTGAAATGATGGAACTCGAGCTGAAAAAGCTGTTTGATACTGTGACGTCCATTCATGATGAGATGTTTTATCTTCGTGAAAGGGAGGAAGAAATGCAACAACTTAATAGAGCAACTAACTCCAAGATGGCGACATTTAGCTTCCTTTCTCTTGTGGTTTGCTTGTCTGTGGCTGGTTTGCAATTATGGCATCTCAAGATGTTCTTTGAGAGGAAGAAGCTCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

267

Amino Acids

30.44

Weight (kDa)

7.14

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 78 - 262 1.4e-51 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 549
AciI CCGC 4 cut(s) 224, 235, 443, 446
AclWI GGATC 1 cut(s) 417
AcyI GRCGYC 2 cut(s) 255, 623
AfaI GTAC 2 cut(s) 190, 311
AfiI CCNNNNNNNGG 4 cut(s) 33, 332, 549, 570
AgsI TTSAA 2 cut(s) 248, 581
AhdI GACNNNNNGTC 1 cut(s) 169
AluBI AGCT 6 cut(s) 60, 482, 598, 607, 717, 796
AluI AGCT 6 cut(s) 60, 482, 598, 607, 717, 796
Alw26I GTCTC 5 cut(s) 44, 86, 102, 114, 443
AlwI GGATC 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 1 cut(s) 540
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 552
AsuHPI GGTGA 5 cut(s) 372, 385, 392, 467, 517
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 552
BanI GGYRCC 1 cut(s) 254
BccI CCATC 4 cut(s) 160, 214, 580, 697
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 484
BcoDI GTCTC 5 cut(s) 44, 86, 102, 114, 443
BfaI CTAG 1 cut(s) 802
BfoI RGCGCY 1 cut(s) 258
BfuI GTATCC 1 cut(s) 412
BglII AGATCT 1 cut(s) 21
BisI GCNGC 1 cut(s) 444
BlsI GCNGC 1 cut(s) 445
BmcAI AGTACT 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 552
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 150, 256
BmsI GCATC 3 cut(s) 222, 276, 775
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 755
BsaHI GRCGYC 2 cut(s) 255, 623
BsaI GGTCTC 2 cut(s) 102, 114
BsaJI CCNNGG 5 cut(s) 92, 403, 501, 543, 555
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 332, 549, 570
Bse1I ACTGG 4 cut(s) 457, 527, 535, 554
BseDI CCNNGG 5 cut(s) 92, 403, 501, 543, 555
BseLI CCNNNNNNNGG 4 cut(s) 33, 332, 549, 570
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 4 cut(s) 457, 527, 535, 554
BseRI GAGGAG 2 cut(s) 16, 788
BshFI GGCC 1 cut(s) 542
BshNI GGYRCC 1 cut(s) 254
BsiHKCI CYCGRG 1 cut(s) 593
BslI CCNNNNNNNGG 4 cut(s) 33, 332, 549, 570
BsmAI GTCTC 5 cut(s) 44, 86, 102, 114, 443
BsnI GGCC 1 cut(s) 542
Bso31I GGTCTC 2 cut(s) 102, 114
BsoBI CYCGRG 1 cut(s) 593
Bsp1407I TGTACA 1 cut(s) 188
Bsp143I GATC 5 cut(s) 21, 70, 183, 409, 484
Bsp19I CCATGG 1 cut(s) 403
BspACI CCGC 4 cut(s) 224, 235, 443, 446
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 1 cut(s) 284
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 150, 256
BspPI GGATC 1 cut(s) 417
BspT107I GGYRCC 1 cut(s) 254
BspTNI GGTCTC 2 cut(s) 102, 114
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 4 cut(s) 457, 527, 535, 554
BssECI CCNNGG 5 cut(s) 92, 403, 501, 543, 555
BssMI GATC 5 cut(s) 21, 70, 183, 409, 484
BssNI GRCGYC 2 cut(s) 255, 623
BssT1I CCWWGG 3 cut(s) 403, 543, 555
Bst4CI ACNGT 6 cut(s) 80, 301, 319, 367, 505, 619
Bst6I CTCTTC 1 cut(s) 348
BstACI GRCGYC 2 cut(s) 255, 623
BstAUI TGTACA 1 cut(s) 188
BstDEI CTNAG 1 cut(s) 271
BstDSI CCRYGG 2 cut(s) 403, 501
BstEII GGTNACC 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 5 cut(s) 24, 73, 186, 412, 487
BstMAI GTCTC 5 cut(s) 44, 86, 102, 114, 443
BstMBI GATC 5 cut(s) 21, 70, 183, 409, 484
BstMWI GCNNNNNNNGC 3 cut(s) 440, 604, 763
BstPI GGTNACC 1 cut(s) 373
BstX2I RGATCY 2 cut(s) 21, 409
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 2 cut(s) 21, 409
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 403, 501
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 3 cut(s) 76, 363, 438
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 552
Csp6I GTAC 2 cut(s) 189, 310
CviAII CATG 4 cut(s) 295, 386, 404, 632
CviQI GTAC 2 cut(s) 189, 310
DdeI CTNAG 1 cut(s) 271
DinI GGCGCC 1 cut(s) 256
DpnI GATC 5 cut(s) 23, 72, 185, 411, 486
DpnII GATC 5 cut(s) 21, 70, 183, 409, 484
DriI GACNNNNNGTC 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 348
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 461
Eco130I CCWWGG 3 cut(s) 403, 543, 555
Eco31I GGTCTC 2 cut(s) 102, 114
Eco47I GGWCC 1 cut(s) 552
Eco88I CYCGRG 1 cut(s) 593
Eco91I GGTNACC 1 cut(s) 373
EcoO65I GGTNACC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 403, 543, 555
EcoT22I ATGCAT 1 cut(s) 269
EgeI GGCGCC 1 cut(s) 256
EheI GGCGCC 1 cut(s) 256
ErhI CCWWGG 3 cut(s) 403, 543, 555
FaeI CATG 4 cut(s) 298, 389, 407, 635
FaiI YATR 8 cut(s) 64, 296, 387, 405, 469, 489, 633, 763
FalI AAGNNNNNCTT 2 cut(s) 764, 796
FatI CATG 4 cut(s) 294, 385, 403, 631
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 444
FspBI CTAG 1 cut(s) 802
GlaI GCGC 1 cut(s) 256
GluI GCNGC 1 cut(s) 444
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 1 cut(s) 542
HhaI GCGC 1 cut(s) 257
Hin1I GRCGYC 2 cut(s) 255, 623
Hin1II CATG 4 cut(s) 298, 389, 407, 635
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HinfI GANTC 1 cut(s) 37
HphI GGTGA 5 cut(s) 372, 385, 392, 467, 517
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 7 cut(s) 54, 70, 86, 163, 198, 274, 498
Hpy188III TCNNGA 4 cut(s) 347, 632, 653, 772
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 2 cut(s) 254, 445
HpyCH4III ACNGT 6 cut(s) 80, 301, 319, 367, 505, 619
HpyCH4IV ACGT 1 cut(s) 623
HpyCH4V TGCA 3 cut(s) 267, 673, 757
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 604, 763
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 623
Hsp92I GRCGYC 2 cut(s) 255, 623
Hsp92II CATG 4 cut(s) 298, 389, 407, 635
HspAI GCGC 1 cut(s) 255
KasI GGCGCC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 5 cut(s) 21, 70, 183, 409, 484
LmnI GCTCC 1 cut(s) 801
LpnPI CCDG 8 cut(s) 237, 355, 360, 438, 508, 516, 535, 735
LweI GCATC 3 cut(s) 222, 276, 775
MaeI CTAG 1 cut(s) 802
MaeII ACGT 1 cut(s) 623
MaeIII GTNAC 4 cut(s) 361, 373, 505, 619
MalI GATC 5 cut(s) 23, 72, 185, 411, 486
MboI GATC 5 cut(s) 21, 70, 183, 409, 484
MboII GAAGA 4 cut(s) 335, 641, 677, 802
MflI RGATCY 2 cut(s) 21, 409
MluCI AATT 5 cut(s) 103, 289, 394, 427, 758
Mly113I GGCGCC 1 cut(s) 255
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 521
Mph1103I ATGCAT 1 cut(s) 269
MseI TTAA 3 cut(s) 6, 209, 681
MslI CAYNNNNRTG 1 cut(s) 299
MwoI GCNNNNNNNGC 3 cut(s) 440, 604, 763
NarI GGCGCC 1 cut(s) 255
NcoI CCATGG 1 cut(s) 403
NdeII GATC 5 cut(s) 21, 70, 183, 409, 484
NlaIII CATG 4 cut(s) 298, 389, 407, 635
NlaIV GGNNCC 2 cut(s) 150, 256
NmuCI GTSAC 4 cut(s) 361, 373, 505, 619
NsiI ATGCAT 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 593
PagI TCATGA 1 cut(s) 631
PflMI CCANNNNNTGG 1 cut(s) 549
PkrI GCNGC 1 cut(s) 445
PleI GAGTC 1 cut(s) 45
PluTI GGCGCC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 45
PspEI GGTNACC 1 cut(s) 373
PspN4I GGNNCC 2 cut(s) 150, 256
PspPI GGNCC 1 cut(s) 552
PspXI VCTCGAGB 1 cut(s) 593
PsuI RGATCY 2 cut(s) 21, 409
RsaI GTAC 2 cut(s) 190, 311
RsaNI GTAC 2 cut(s) 189, 310
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 3 cut(s) 6, 209, 681
SatI GCNGC 1 cut(s) 444
Sau3AI GATC 5 cut(s) 21, 70, 183, 409, 484
Sau96I GGNCC 1 cut(s) 552
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 1 cut(s) 46
SfaNI GCATC 3 cut(s) 222, 276, 775
SfoI GGCGCC 1 cut(s) 256
Sfr274I CTCGAG 1 cut(s) 593
SinI GGWCC 1 cut(s) 552
SlaI CTCGAG 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 593, 770
SmoI CTYRAG 2 cut(s) 593, 770
Sse9I AATT 5 cut(s) 103, 289, 394, 427, 758
SsiI CCGC 4 cut(s) 224, 235, 443, 446
SspDI GGCGCC 1 cut(s) 254
SspI AATATT 1 cut(s) 180
SspMI CTAG 1 cut(s) 802
StyI CCWWGG 3 cut(s) 403, 543, 555
TaaI ACNGT 6 cut(s) 80, 301, 319, 367, 505, 619
TaiI ACGT 1 cut(s) 626
TaqI TCGA 3 cut(s) 230, 240, 594
TaqII GACCGA 1 cut(s) 84
TasI AATT 5 cut(s) 103, 289, 394, 427, 758
TatI WGTACW 2 cut(s) 188, 309
TauI GCSGC 1 cut(s) 446
Tru1I TTAA 3 cut(s) 6, 209, 681
Tru9I TTAA 3 cut(s) 6, 209, 681
TscAI CASTG 3 cut(s) 83, 370, 445
TseFI GTSAC 4 cut(s) 361, 373, 505, 619
Tsp45I GTSAC 4 cut(s) 361, 373, 505, 619
TspDTI ATGAA 1 cut(s) 620
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 3 cut(s) 83, 370, 445
Van91I CCANNNNNTGG 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 552
XhoI CTCGAG 1 cut(s) 593
XspI CTAG 1 cut(s) 802
ZraI GACGTC 1 cut(s) 624
ZrmI AGTACT 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.