RchiOBHm_Chr2g0167341

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001491
Physical Location & Seq
Reverse (-)
81897733 .. 81900034
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53513

Sequence Viewer

Length: 819 bp
ATGCTTAGAAACCTAGTCTGTTTAACAAACACAAACAGATCTCCTCCGTTTGGAGTCTCTCTCTTCCGAGAAGCTCAAACTCCGATCAACACTGTCTCCCAACGCCTCGGTTCTCCAAATTGGTCTCCCCACCAACTCAAAACCCTCATTGTTTTCTCATTGGAACCATCACTTTCTGACACAATGTCGCCAATATTGATCTGTACATTTCTGATATTGTGCTTAATGTCAACGATGGCGGTTTCGATGCGGTTCGACCTTCAATCTGGCGCCACTAAATGCATCTCAGACGAAATAAAGAGCAATTCCATGACTGTGGGCAAGTACTCCACCGTCAATCCCAATGAGGGCTTTCCTCTTCCTGATACGCACAAAGTCACTGTCAGGGTGACCTCACCTAATGGCAATAATTATCACCATGGGGATCTTGTGGATACGGGTAATTTTGCCTTCACTGCGGCGGAGACCGGTGATTATTCAGTATGCTTTTGGGTAGTTGATCATAAGCCTCCGACCACGGTCACGATTGATTTTGACTGGAAAACTGGAGTTGAGGCCAAGGACTGGTCCAAGGTTGCCAAGAAAGGGCAGATTGAAATGATGGAACTCGAGCTGAAAAAGCTGTTTGATACTGTGACGTCCATTCATGATGAGATGTTTTATCTTCGTGAAAGGGAGGAAGAAATGCAACAACTTAATAGAGCAACTAACTCCAAGATGGCGACGTTTAGCTTCCTTTCTCTTGTAGTTTGCTTGTCTGTGGCCGGTTTGCAATTATGGCATCTCAAGATGTTCTTTGAGAGGAAGAAGCTCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

272

Amino Acids

30.75

Weight (kDa)

6.65

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 83 - 267 6.3e-52 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 564
AciI CCGC 4 cut(s) 239, 250, 458, 461
AclWI GGATC 1 cut(s) 432
AcoI YGGCCR 1 cut(s) 762
AcyI GRCGYC 2 cut(s) 270, 638
AfaI GTAC 2 cut(s) 205, 326
AfiI CCNNNNNNNGG 4 cut(s) 50, 347, 564, 585
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 2 cut(s) 263, 596
AhdI GACNNNNNGTC 1 cut(s) 184
AluBI AGCT 5 cut(s) 74, 613, 622, 732, 811
AluI AGCT 5 cut(s) 74, 613, 622, 732, 811
Alw26I GTCTC 4 cut(s) 61, 100, 129, 458
AlwI GGATC 1 cut(s) 432
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 2 cut(s) 555, 762
ArsI GACNNNNNNTTYG 2 cut(s) 366, 398
AsiGI ACCGGT 1 cut(s) 467
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 567
AsuHPI GGTGA 4 cut(s) 387, 400, 407, 482
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 1 cut(s) 567
BanI GGYRCC 1 cut(s) 269
BccI CCATC 4 cut(s) 175, 229, 595, 712
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 4 cut(s) 61, 100, 129, 458
BfaI CTAG 2 cut(s) 14, 817
BfoI RGCGCY 1 cut(s) 273
BfuI GTATCC 1 cut(s) 427
BglII AGATCT 1 cut(s) 38
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
BmcAI AGTACT 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 567
BmeRI GACNNNNNGTC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 567
BmiI GGNNCC 2 cut(s) 165, 271
BmsI GCATC 3 cut(s) 237, 291, 790
BoxI GACNNNNGTC 1 cut(s) 518
BplI GAGNNNNNCTC 2 cut(s) 45, 77
BpmI CTGGAG 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 770
BsaHI GRCGYC 2 cut(s) 270, 638
BsaI GGTCTC 2 cut(s) 129, 458
BsaJI CCNNGG 5 cut(s) 106, 418, 516, 558, 570
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 347, 564, 585
Bse118I RCCGGY 2 cut(s) 467, 764
Bse1I ACTGG 3 cut(s) 542, 550, 569
BseDI CCNNGG 5 cut(s) 106, 418, 516, 558, 570
BseLI CCNNNNNNNGG 4 cut(s) 50, 347, 564, 585
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 3 cut(s) 542, 550, 569
BseRI GAGGAG 2 cut(s) 33, 803
BshFI GGCC 2 cut(s) 557, 764
BshNI GGYRCC 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 467
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 2 cut(s) 468, 765
BslI CCNNNNNNNGG 4 cut(s) 50, 347, 564, 585
BsmAI GTCTC 4 cut(s) 61, 100, 129, 458
BsnI GGCC 2 cut(s) 557, 764
Bso31I GGTCTC 2 cut(s) 129, 458
BsoBI CYCGRG 1 cut(s) 608
Bsp1407I TGTACA 1 cut(s) 203
Bsp143I GATC 5 cut(s) 38, 84, 198, 424, 499
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 4 cut(s) 239, 250, 458, 461
BspANI GGCC 2 cut(s) 557, 764
BspCNI CTCAG 1 cut(s) 299
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 165, 271
BspPI GGATC 1 cut(s) 432
BspT107I GGYRCC 1 cut(s) 269
BspTNI GGTCTC 2 cut(s) 129, 458
BsrFI RCCGGY 2 cut(s) 467, 764
BsrGI TGTACA 1 cut(s) 203
BsrI ACTGG 3 cut(s) 542, 550, 569
BssAI RCCGGY 2 cut(s) 467, 764
BssECI CCNNGG 5 cut(s) 106, 418, 516, 558, 570
BssMI GATC 5 cut(s) 38, 84, 198, 424, 499
BssNI GRCGYC 2 cut(s) 270, 638
BssT1I CCWWGG 3 cut(s) 418, 558, 570
Bst4CI ACNGT 6 cut(s) 94, 316, 334, 382, 520, 634
Bst6I CTCTTC 2 cut(s) 68, 363
BstACI GRCGYC 2 cut(s) 270, 638
BstAUI TGTACA 1 cut(s) 203
BstDEI CTNAG 2 cut(s) 5, 286
BstDSI CCRYGG 2 cut(s) 418, 516
BstEII GGTNACC 1 cut(s) 388
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 5 cut(s) 41, 87, 201, 427, 502
BstMAI GTCTC 4 cut(s) 61, 100, 129, 458
BstMBI GATC 5 cut(s) 38, 84, 198, 424, 499
BstMWI GCNNNNNNNGC 3 cut(s) 455, 619, 778
BstPAI GACNNNNGTC 1 cut(s) 518
BstPI GGTNACC 1 cut(s) 388
BstX2I RGATCY 2 cut(s) 38, 424
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 2 cut(s) 38, 424
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 557, 764
BtgI CCRYGG 2 cut(s) 418, 516
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 3 cut(s) 90, 378, 453
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 2 cut(s) 467, 764
Cfr13I GGNCC 1 cut(s) 567
Csp6I GTAC 2 cut(s) 204, 325
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 3 cut(s) 310, 419, 647
CviJI RGCY 9 cut(s) 74, 351, 508, 557, 613, 622, 732, 764, 811
CviKI_1 RGCY 9 cut(s) 74, 351, 508, 557, 613, 622, 732, 764, 811
CviQI GTAC 2 cut(s) 204, 325
DdeI CTNAG 2 cut(s) 5, 286
DinI GGCGCC 1 cut(s) 271
DpnI GATC 5 cut(s) 40, 86, 200, 426, 501
DpnII GATC 5 cut(s) 38, 84, 198, 424, 499
DriI GACNNNNNGTC 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 2 cut(s) 68, 363
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 2 cut(s) 68, 363
EciI GGCGGA 1 cut(s) 476
Eco130I CCWWGG 3 cut(s) 418, 558, 570
Eco31I GGTCTC 2 cut(s) 129, 458
Eco47I GGWCC 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 608
Eco91I GGTNACC 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 388
EcoT14I CCWWGG 3 cut(s) 418, 558, 570
EcoT22I ATGCAT 1 cut(s) 284
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
ErhI CCWWGG 3 cut(s) 418, 558, 570
FaeI CATG 3 cut(s) 313, 422, 650
FaiI YATR 6 cut(s) 311, 420, 484, 504, 648, 778
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FatI CATG 3 cut(s) 309, 418, 646
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 1 cut(s) 459
Fsp4HI GCNGC 1 cut(s) 459
FspBI CTAG 2 cut(s) 14, 817
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 459
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 2 cut(s) 557, 764
HapII CCGG 2 cut(s) 468, 765
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 2 cut(s) 270, 638
Hin1II CATG 3 cut(s) 313, 422, 650
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 1 cut(s) 54
HpaII CCGG 2 cut(s) 468, 765
HphI GGTGA 4 cut(s) 387, 400, 407, 482
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 6 cut(s) 68, 84, 178, 213, 289, 513
Hpy188III TCNNGA 5 cut(s) 362, 523, 647, 668, 787
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 1 cut(s) 727
HpyAV CCTTC 2 cut(s) 269, 460
HpyCH4III ACNGT 6 cut(s) 94, 316, 334, 382, 520, 634
HpyCH4IV ACGT 2 cut(s) 638, 725
HpyCH4V TGCA 3 cut(s) 282, 688, 772
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 619, 778
HpyF3I CTNAG 2 cut(s) 5, 286
HpySE526I ACGT 2 cut(s) 638, 725
Hsp92I GRCGYC 2 cut(s) 270, 638
Hsp92II CATG 3 cut(s) 313, 422, 650
HspAI GCGC 1 cut(s) 270
KasI GGCGCC 1 cut(s) 269
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 5 cut(s) 38, 84, 198, 424, 499
LmnI GCTCC 1 cut(s) 816
LpnPI CCDG 8 cut(s) 252, 370, 375, 481, 523, 531, 550, 778
LweI GCATC 3 cut(s) 237, 291, 790
MaeI CTAG 2 cut(s) 14, 817
MaeII ACGT 2 cut(s) 638, 725
MaeIII GTNAC 4 cut(s) 376, 388, 520, 634
MalI GATC 5 cut(s) 40, 86, 200, 426, 501
MboI GATC 5 cut(s) 38, 84, 198, 424, 499
MboII GAAGA 5 cut(s) 55, 350, 656, 692, 817
MflI RGATCY 2 cut(s) 38, 424
MluCI AATT 5 cut(s) 118, 304, 409, 442, 773
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 536
Mph1103I ATGCAT 1 cut(s) 284
MseI TTAA 3 cut(s) 23, 224, 696
MslI CAYNNNNRTG 1 cut(s) 314
MspI CCGG 2 cut(s) 468, 765
MwoI GCNNNNNNNGC 3 cut(s) 455, 619, 778
NarI GGCGCC 1 cut(s) 270
NcoI CCATGG 1 cut(s) 418
NdeII GATC 5 cut(s) 38, 84, 198, 424, 499
NlaIII CATG 3 cut(s) 313, 422, 650
NlaIV GGNNCC 2 cut(s) 165, 271
NmuCI GTSAC 4 cut(s) 376, 388, 520, 634
NsiI ATGCAT 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 608
PagI TCATGA 1 cut(s) 646
PflMI CCANNNNNTGG 1 cut(s) 564
PinAI ACCGGT 1 cut(s) 467
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 62
PshAI GACNNNNGTC 1 cut(s) 518
PspEI GGTNACC 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 165, 271
PspPI GGNCC 1 cut(s) 567
PspXI VCTCGAGB 1 cut(s) 608
PsuI RGATCY 2 cut(s) 38, 424
RsaI GTAC 2 cut(s) 205, 326
RsaNI GTAC 2 cut(s) 204, 325
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 3 cut(s) 23, 224, 696
SatI GCNGC 1 cut(s) 459
Sau3AI GATC 5 cut(s) 38, 84, 198, 424, 499
Sau96I GGNCC 1 cut(s) 567
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 1 cut(s) 63
SfaNI GCATC 3 cut(s) 237, 291, 790
SfoI GGCGCC 1 cut(s) 271
Sfr274I CTCGAG 1 cut(s) 608
SinI GGWCC 1 cut(s) 567
SlaI CTCGAG 1 cut(s) 608
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 608, 785
SmoI CTYRAG 2 cut(s) 608, 785
Sse9I AATT 5 cut(s) 118, 304, 409, 442, 773
SsiI CCGC 4 cut(s) 239, 250, 458, 461
SspDI GGCGCC 1 cut(s) 269
SspI AATATT 1 cut(s) 195
SspMI CTAG 2 cut(s) 14, 817
StyI CCWWGG 3 cut(s) 418, 558, 570
TaaI ACNGT 6 cut(s) 94, 316, 334, 382, 520, 634
TaiI ACGT 2 cut(s) 641, 728
TaqI TCGA 3 cut(s) 245, 255, 609
TasI AATT 5 cut(s) 118, 304, 409, 442, 773
TatI WGTACW 2 cut(s) 203, 324
TauI GCSGC 1 cut(s) 461
Tru1I TTAA 3 cut(s) 23, 224, 696
Tru9I TTAA 3 cut(s) 23, 224, 696
TscAI CASTG 3 cut(s) 97, 385, 460
TseFI GTSAC 4 cut(s) 376, 388, 520, 634
Tsp45I GTSAC 4 cut(s) 376, 388, 520, 634
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 3 cut(s) 97, 385, 460
Van91I CCANNNNNTGG 1 cut(s) 564
VpaK11BI GGWCC 1 cut(s) 567
XhoI CTCGAG 1 cut(s) 608
XspI CTAG 2 cut(s) 14, 817
ZraI GACGTC 1 cut(s) 639
ZrmI AGTACT 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.