pycom06g09850

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001687
Physical Location & Seq
Forward (+)
15176008 .. 15177964
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1155 bp
ATGGAGAACAAAAACTCTAGAGTTTGTGTTACAGGTGCTTCTGGATTCATTGCCTCTTCACTCGTTGAAAAACTCCTACAGCAGGGCCACACAGTCCACGCCACCCTCAGGAACTTGGATGATGAGTCGAAAGTTGGGCTTCTCAAGTCCCTTCCTAATGCGGACACCAAATTGTTGCTGTTCCAAGCTGACCTATATGACCCTCAAGGGTTTGAGCCTGCCATTGAAGGGTGTGAGTTTGTTTTCCACGTTGCCACTCCAATGCAGCATAACAACCTAAGCACTCAGTACAAGGACACAGATGAAGCTGCTGTTGCTGGGGTGAGAATCATTGCTGATTCCTGCATTCGTTCGCAGACCGTCAAGCGACTCATCTACACTGCAAGCATACTAGCTGCGTCGCCTCGGACAGAAGATGGGGCTGGATTCAAACCCTACGTTGATGAATCATGTTGGACACCCCTTGATGTCTCATTTCCTCATGGCACTGATTTCTCAATGGGGTATATCGTATCAAAGACATTAGCAGAGAAAGCAGTGCTGAGCTATAACGACATTGATGGTGGTAAATTAGAAGCAGTAACTCTTCCTTGTGGCCTAGTAGGAGGAGAAACTCTTCTCCCAAATTCGCCTCTAAGTGTGGGAGTAATTATATCACAACTTACCGGGGACTTGAACGGTTACAATGCATTAAAATTTATGCAAGAAGTTATCGGTTCTGTTCCTCTTGTACACATTGAAGATGTTTGCCGAGCACATATCTTCTGCATGGAACAACCATCGATGAGAGGCAGATTCTGCTGTGCAGTTGCCAGTCCGAGTATCAAAGAAATCGCTGCCTGCTTCCAAGAAAACTACCCGGAATACAAGATAGCAAAAGAATTCGCCGAAGGACCAGAGGAAGGAATCAAATGTGACTTTACTAAGCTGGTGAAGATCGGTTTTGAGTACAAGTATGGTATGAAGAACATACTTGATGACAGTGTTGCAAGCGGAAGGAGCTGCAAAAGTGTAAAAAAACACACTGATGACTACAAGCAGAAGAAGCAAGCAAATTTTGGAGACGAAACACTTTGGTTTCCCGTGGTTGGAGTAAAGGTTCTGCTTCGAGGTCTTTTAATGGTGAAGAACACAAAGTTAAGGGACTATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

385

Amino Acids

42.2

Weight (kDa)

5.96

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 109 5.8e-11 NmrA-like family
Epimerase PF01370 8 - 261 3.2e-17 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 204 7.2e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 186 8.6e-09 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 87 4.2e-07 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 136 1.8e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 161, 993
AcsI RAATTY 4 cut(s) 625, 695, 881, 1054
AfaI GTAC 3 cut(s) 290, 732, 950
AfiI CCNNNNNNNGG 5 cut(s) 82, 108, 228, 902, 1088
AgsI TTSAA 5 cut(s) 68, 227, 430, 676, 740
AloI GAACNNNNNNTCC 2 cut(s) 1083, 1115
AluBI AGCT 6 cut(s) 188, 308, 395, 546, 928, 1002
AluI AGCT 6 cut(s) 188, 308, 395, 546, 928, 1002
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 2 cut(s) 475, 1056
AlwNI CAGNNNCTG 1 cut(s) 798
AoxI GGCC 2 cut(s) 85, 595
ApeKI GCWGC 5 cut(s) 265, 308, 395, 836, 1002
ApoI RAATTY 4 cut(s) 625, 695, 881, 1054
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 2 cut(s) 85, 893
AsuC2I CCSGG 2 cut(s) 667, 860
AsuHPI GGTGA 3 cut(s) 334, 943, 1135
AvaII GGWCC 1 cut(s) 893
AxyI CCTNAGG 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 5 cut(s) 277, 295, 382, 823, 989
BccI CCATC 3 cut(s) 410, 554, 787
BcnI CCSGG 2 cut(s) 667, 860
BcoDI GTCTC 2 cut(s) 475, 1056
BfaI CTAG 3 cut(s) 18, 392, 599
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 5 cut(s) 266, 309, 396, 837, 1003
BlpI GCTNAGC 1 cut(s) 542
BlsI GCNGC 5 cut(s) 267, 310, 397, 838, 1004
Bme1390I CCNGG 2 cut(s) 667, 860
Bme18I GGWCC 1 cut(s) 893
BmgT120I GGNCC 2 cut(s) 85, 893
BmrFI CCNGG 2 cut(s) 667, 860
Bpu10I CCTNAGC 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 542
BpuEI CTTGAG 2 cut(s) 128, 189
BpuMI CCSGG 2 cut(s) 667, 860
Bsa29I ATCGAT 1 cut(s) 782
BsaJI CCNNGG 3 cut(s) 404, 666, 1083
BsaXI ACNNNNNCTCC 2 cut(s) 1083, 1113
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 108, 228, 902, 1088
Bse1I ACTGG 1 cut(s) 813
Bse21I CCTNAGG 1 cut(s) 107
Bse3DI GCAATG 2 cut(s) 48, 330
BseCI ATCGAT 1 cut(s) 782
BseDI CCNNGG 3 cut(s) 404, 666, 1083
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 5 cut(s) 82, 108, 228, 902, 1088
BseMI GCAATG 2 cut(s) 48, 330
BseMII CTCAG 3 cut(s) 121, 299, 533
BseNI ACTGG 1 cut(s) 813
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 5 cut(s) 277, 295, 382, 823, 989
BseYI CCCAGC 1 cut(s) 317
BsgI GTGCAG 1 cut(s) 825
BshFI GGCC 2 cut(s) 87, 597
BshVI ATCGAT 1 cut(s) 782
BsiHKAI GWGCWC 1 cut(s) 757
BsiSI CCGG 2 cut(s) 666, 860
BslFI GGGAC 2 cut(s) 133, 683
BslI CCNNNNNNNGG 5 cut(s) 82, 108, 228, 902, 1088
BsmAI GTCTC 2 cut(s) 475, 1056
BsmBI CGTCTC 1 cut(s) 1056
BsmFI GGGAC 2 cut(s) 133, 683
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 2 cut(s) 87, 597
Bsp1286I GDGCHC 1 cut(s) 757
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 1 cut(s) 936
Bsp1720I GCTNAGC 1 cut(s) 542
BspACI CCGC 2 cut(s) 161, 993
BspANI GGCC 2 cut(s) 87, 597
BspCNI CTCAG 3 cut(s) 120, 298, 534
BspDI ATCGAT 1 cut(s) 782
BsrDI GCAATG 2 cut(s) 48, 330
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 1 cut(s) 813
BssECI CCNNGG 3 cut(s) 404, 666, 1083
BssMI GATC 1 cut(s) 936
Bst4CI ACNGT 4 cut(s) 94, 361, 680, 983
Bst6I CTCTTC 3 cut(s) 61, 591, 621
BstAPI GCANNNNNTGC 1 cut(s) 798
BstAUI TGTACA 1 cut(s) 730
BstC8I GCNNGC 5 cut(s) 219, 385, 841, 991, 1050
BstDEI CTNAG 6 cut(s) 107, 278, 285, 542, 635, 924
BstDSI CCRYGG 1 cut(s) 1083
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 939
BstMAI GTCTC 2 cut(s) 475, 1056
BstMBI GATC 1 cut(s) 936
BstMWI GCNNNNNNNGC 5 cut(s) 314, 533, 798, 999, 1045
BstSCI CCNGG 2 cut(s) 665, 858
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 5 cut(s) 277, 295, 382, 823, 989
Bsu15I ATCGAT 1 cut(s) 782
Bsu36I CCTNAGG 1 cut(s) 107
BsuRI GGCC 2 cut(s) 87, 597
BsuTUI ATCGAT 1 cut(s) 782
BtgI CCRYGG 1 cut(s) 1083
BtsCI GGATG 1 cut(s) 124
BtsI GCAGTG 2 cut(s) 378, 543
BtsIMutI CAGTG 5 cut(s) 378, 486, 543, 988, 1023
Cac8I GCNNGC 5 cut(s) 219, 385, 841, 991, 1050
CaiI CAGNNNCTG 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 85, 893
ClaI ATCGAT 1 cut(s) 782
CseI GACGC 1 cut(s) 387
Csp6I GTAC 3 cut(s) 289, 731, 949
CviAII CATG 3 cut(s) 450, 482, 769
CviQI GTAC 3 cut(s) 289, 731, 949
DdeI CTNAG 6 cut(s) 107, 278, 285, 542, 635, 924
DpnI GATC 1 cut(s) 938
DpnII GATC 1 cut(s) 936
Eam1104I CTCTTC 3 cut(s) 61, 591, 621
EarI CTCTTC 3 cut(s) 61, 591, 621
Eco47I GGWCC 1 cut(s) 893
Eco81I CCTNAGG 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 881
EcoT22I ATGCAT 1 cut(s) 691
Esp3I CGTCTC 1 cut(s) 1056
FaeI CATG 3 cut(s) 453, 485, 772
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 2 cut(s) 133, 683
FatI CATG 3 cut(s) 449, 481, 768
Fnu4HI GCNGC 5 cut(s) 266, 309, 396, 837, 1003
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 5 cut(s) 266, 309, 396, 837, 1003
FspBI CTAG 3 cut(s) 18, 392, 599
GluI GCNGC 5 cut(s) 266, 309, 396, 837, 1003
GsaI CCCAGC 1 cut(s) 321
HaeIII GGCC 2 cut(s) 87, 597
HapII CCGG 2 cut(s) 666, 860
HgaI GACGC 1 cut(s) 387
Hin1II CATG 3 cut(s) 453, 485, 772
HinfI GANTC 9 cut(s) 45, 125, 327, 338, 369, 426, 446, 795, 906
HpaII CCGG 2 cut(s) 666, 860
HphI GGTGA 3 cut(s) 334, 943, 1135
Hpy166II GTNNAC 2 cut(s) 97, 733
Hpy188I TCNGA 2 cut(s) 408, 819
Hpy188III TCNNGA 3 cut(s) 18, 42, 109
Hpy8I GTNNAC 2 cut(s) 97, 733
Hpy99I CGWCG 1 cut(s) 403
HpyAV CCTTC 5 cut(s) 161, 221, 884, 896, 990
HpyCH4III ACNGT 4 cut(s) 94, 361, 680, 983
HpyCH4IV ACGT 2 cut(s) 249, 438
HpyCH4V TGCA 9 cut(s) 265, 345, 383, 689, 703, 768, 806, 989, 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 314, 533, 798, 999, 1045
HpyF3I CTNAG 6 cut(s) 107, 278, 285, 542, 635, 924
HpySE526I ACGT 2 cut(s) 249, 438
Hsp92II CATG 3 cut(s) 453, 485, 772
Kzo9I GATC 1 cut(s) 936
LmnI GCTCC 1 cut(s) 999
Lsp1109I GCAGC 5 cut(s) 277, 295, 382, 823, 989
MaeI CTAG 3 cut(s) 18, 392, 599
MaeII ACGT 2 cut(s) 249, 438
MaeIII GTNAC 4 cut(s) 28, 580, 680, 914
MalI GATC 1 cut(s) 938
MboI GATC 1 cut(s) 936
MhlI GDGCHC 1 cut(s) 757
MluCI AATT 7 cut(s) 170, 569, 625, 648, 695, 881, 1054
MlyI GAGTC 2 cut(s) 134, 363
MmeI TCCRAC 2 cut(s) 434, 1069
Mph1103I ATGCAT 1 cut(s) 691
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 3 cut(s) 692, 1118, 1139
MslI CAYNNNNRTG 2 cut(s) 260, 1026
MspI CCGG 2 cut(s) 666, 860
MspR9I CCNGG 2 cut(s) 667, 860
Mva1269I GAATGC 1 cut(s) 345
MwoI GCNNNNNNNGC 5 cut(s) 314, 533, 798, 999, 1045
NciI CCSGG 2 cut(s) 667, 860
NdeII GATC 1 cut(s) 936
NlaIII CATG 3 cut(s) 453, 485, 772
NmeAIII GCCGAG 1 cut(s) 776
NmuCI GTSAC 1 cut(s) 914
NsiI ATGCAT 1 cut(s) 691
PctI GAATGC 1 cut(s) 345
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 7 cut(s) 45, 327, 338, 426, 446, 795, 906
PkrI GCNGC 5 cut(s) 267, 310, 397, 838, 1004
PleI GAGTC 2 cut(s) 133, 363
PpsI GAGTC 2 cut(s) 133, 363
PspFI CCCAGC 1 cut(s) 317
PspPI GGNCC 2 cut(s) 85, 893
PstNI CAGNNNCTG 1 cut(s) 798
RsaI GTAC 3 cut(s) 290, 732, 950
RsaNI GTAC 3 cut(s) 289, 731, 949
RseI CAYNNNNRTG 2 cut(s) 260, 1026
SaqAI TTAA 3 cut(s) 692, 1118, 1139
SatI GCNGC 5 cut(s) 266, 309, 396, 837, 1003
Sau3AI GATC 1 cut(s) 936
Sau96I GGNCC 2 cut(s) 85, 893
SchI GAGTC 2 cut(s) 134, 363
ScrFI CCNGG 2 cut(s) 667, 860
SduI GDGCHC 1 cut(s) 757
SfcI CTRYAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 893
SmiMI CAYNNNNRTG 2 cut(s) 260, 1026
SmlI CTYRAG 2 cut(s) 143, 204
SmoI CTYRAG 2 cut(s) 143, 204
Sse9I AATT 7 cut(s) 170, 569, 625, 648, 695, 881, 1054
SsiI CCGC 2 cut(s) 161, 993
SspMI CTAG 3 cut(s) 18, 392, 599
StyD4I CCNGG 2 cut(s) 665, 858
TaaI ACNGT 4 cut(s) 94, 361, 680, 983
TaiI ACGT 2 cut(s) 252, 441
TaqI TCGA 3 cut(s) 128, 782, 1108
TasI AATT 7 cut(s) 170, 569, 625, 648, 695, 881, 1054
TatI WGTACW 3 cut(s) 288, 730, 948
TfiI GAWTC 7 cut(s) 45, 327, 338, 426, 446, 795, 906
Tru1I TTAA 3 cut(s) 692, 1118, 1139
Tru9I TTAA 3 cut(s) 692, 1118, 1139
TscAI CASTG 5 cut(s) 385, 493, 543, 988, 1030
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 5 cut(s) 265, 308, 395, 836, 1002
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 4 cut(s) 37, 318, 459, 977
TspRI CASTG 5 cut(s) 385, 493, 543, 988, 1030
VpaK11BI GGWCC 1 cut(s) 893
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 4 cut(s) 625, 695, 881, 1054
XbaI TCTAGA 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 3 cut(s) 18, 392, 599
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.