pycom10g22490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002208
Physical Location & Seq
Reverse (-)
24862983 .. 24864193
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22490.3

Sequence Viewer

Length: 762 bp
ATGCATTTATTAGATAAAAGGTCGACGCAGGCTCTGCCTGTTGCTCTGATGATTCATGATAACTCGACGGATCGATCACAGCCATCGTGCTGGCGACGCATCATTCAAATATCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTGACGGTTGGACCTTGGGTTGGGTCGATCGATCCGCCTCTGGTGTGCACCAGTCGTCTCGTCCCTTCTACTGGCGATACGCTCCTGGCCTTAATTGGTCGGGTCATGCCTTCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTTAAAGCAAGCCTACGCTTTTAATACATTAGCATTAGATAACATCATAGGATTTCGGTTATATTCTGTTGCATTTGCCAATGATGTTTTCATTAATCAAGAACGAAAGTTGGAGGCTCGAGGACGATCAGATATCGTCCTACTCTCAACCATAAACGATGCCTACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGTCAGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGATCGCAAGGCTAAAACTTAAAGGAATTGACGGAAGAGCACCACCAGAAGTGGAGCCTCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGAAGCGATTTGTCTAGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCAGAGGTCATCATTCGTGGCCAGCTTCTTAGAGGGACTATAACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

254

Amino Acids

27.87

Weight (kDa)

8.99

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AciI CCGC 2 cut(s) 192, 476
AclWI GGATC 3 cut(s) 78, 183, 478
AcoI YGGCCR 2 cut(s) 635, 730
AfiI CCNNNNNNNGG 3 cut(s) 177, 228, 472
AgsI TTSAA 2 cut(s) 107, 286
AjnI CCWGG 2 cut(s) 241, 465
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 2 cut(s) 708, 736
AluI AGCT 2 cut(s) 708, 736
Alw21I GWGCWC 2 cut(s) 207, 586
Alw26I GTCTC 2 cut(s) 219, 681
Alw44I GTGCAC 1 cut(s) 203
AlwI GGATC 3 cut(s) 78, 183, 478
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 4 cut(s) 144, 244, 635, 730
ApaLI GTGCAC 1 cut(s) 203
AseI ATTAAT 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 168
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 2 cut(s) 170, 662
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 168
BaeGI GKGCMC 1 cut(s) 207
BalI TGGCCA 1 cut(s) 732
Bbv12I GWGCWC 2 cut(s) 207, 586
BbvCI CCTCAGC 1 cut(s) 692
BccI CCATC 2 cut(s) 91, 125
BceAI ACGGC 1 cut(s) 622
BciT130I CCWGG 2 cut(s) 243, 467
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 219, 681
BfaI CTAG 2 cut(s) 665, 705
Bme1390I CCNGG 3 cut(s) 243, 467, 535
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 532, 600
BmrFI CCNGG 3 cut(s) 243, 467, 535
BmsI GCATC 2 cut(s) 108, 448
Bpu10I CCTNAGC 1 cut(s) 692
BpuMI CCSGG 1 cut(s) 535
Bsa29I ATCGAT 2 cut(s) 73, 187
BsaI GGTCTC 1 cut(s) 681
BsaJI CCNNGG 4 cut(s) 151, 171, 466, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 228, 472
Bse1I ACTGG 2 cut(s) 208, 233
BseBI CCWGG 2 cut(s) 243, 467
BseCI ATCGAT 2 cut(s) 73, 187
BseDI CCNNGG 4 cut(s) 151, 171, 466, 534
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 3 cut(s) 177, 228, 472
BseMII CTCAG 1 cut(s) 706
BseNI ACTGG 2 cut(s) 208, 233
BseSI GKGCMC 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 4 cut(s) 146, 246, 637, 732
BshVI ATCGAT 2 cut(s) 73, 187
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 207, 586
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 1 cut(s) 534
BslFI GGGAC 1 cut(s) 204
BslI CCNNNNNNNGG 3 cut(s) 177, 228, 472
BsmAI GTCTC 2 cut(s) 219, 681
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 204
BsnI GGCC 4 cut(s) 146, 246, 637, 732
Bso31I GGTCTC 1 cut(s) 681
BsoBI CYCGRG 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 207, 586
Bsp143I GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 2 cut(s) 192, 476
BspANI GGCC 4 cut(s) 146, 246, 637, 732
BspCNI CTCAG 1 cut(s) 705
BspDI ATCGAT 2 cut(s) 73, 187
BspHI TCATGA 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 532, 600
BspPI GGATC 3 cut(s) 78, 183, 478
BspQI GCTCTTC 1 cut(s) 574
BspTNI GGTCTC 1 cut(s) 681
BsrI ACTGG 2 cut(s) 208, 233
BssECI CCNNGG 4 cut(s) 151, 171, 466, 534
BssMI GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
BssT1I CCWWGG 2 cut(s) 151, 171
Bst2UI CCWGG 2 cut(s) 243, 467
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 34
BstC8I GCNNGC 5 cut(s) 30, 92, 309, 698, 734
BstDEI CTNAG 4 cut(s) 643, 692, 740, 759
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 484
BstKTI GATC 7 cut(s) 73, 77, 187, 191, 428, 473, 549
BstMAI GTCTC 2 cut(s) 219, 681
BstMBI GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 34, 96, 272
BstNI CCWGG 2 cut(s) 243, 467
BstSCI CCNGG 3 cut(s) 241, 465, 533
BstSLI GKGCMC 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 90
Bsu15I ATCGAT 2 cut(s) 73, 187
BsuRI GGCC 4 cut(s) 146, 246, 637, 732
BsuTUI ATCGAT 2 cut(s) 73, 187
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 484
Cac8I GCNNGC 5 cut(s) 30, 92, 309, 698, 734
CaiI CAGNNNCTG 1 cut(s) 34
CciI TCATGA 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 168
ClaI ATCGAT 2 cut(s) 73, 187
CseI GACGC 2 cut(s) 34, 105
CviAII CATG 4 cut(s) 56, 152, 263, 633
DdeI CTNAG 4 cut(s) 643, 692, 740, 759
DpnI GATC 7 cut(s) 72, 76, 186, 190, 427, 472, 548
DpnII GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
EaeI YGGCCR 2 cut(s) 635, 730
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
EciI GGCGGA 2 cut(s) 181, 491
Eco130I CCWWGG 2 cut(s) 151, 171
Eco31I GGTCTC 1 cut(s) 681
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 417
EcoRII CCWGG 2 cut(s) 241, 465
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 151, 171
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 151, 171
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 4 cut(s) 59, 155, 266, 636
FaqI GGGAC 1 cut(s) 204
FatI CATG 4 cut(s) 55, 151, 262, 632
FblI GTMKAC 1 cut(s) 23
FokI GGATG 1 cut(s) 491
FspBI CTAG 2 cut(s) 665, 705
HaeIII GGCC 4 cut(s) 146, 246, 637, 732
HapII CCGG 1 cut(s) 534
HgaI GACGC 2 cut(s) 34, 105
Hin1II CATG 4 cut(s) 59, 155, 266, 636
HincII GTYRAC 2 cut(s) 24, 679
HindII GTYRAC 2 cut(s) 24, 679
HinfI GANTC 3 cut(s) 52, 493, 614
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 1 cut(s) 534
HphI GGTGA 2 cut(s) 170, 662
Hpy166II GTNNAC 3 cut(s) 24, 205, 679
Hpy188I TCNGA 2 cut(s) 48, 430
Hpy188III TCNNGA 3 cut(s) 56, 398, 611
Hpy8I GTNNAC 3 cut(s) 24, 205, 679
Hpy99I CGWCG 3 cut(s) 28, 70, 99
HpyAV CCTTC 2 cut(s) 232, 277
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 5 cut(s) 4, 205, 371, 632, 713
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 96, 272
HpyF3I CTNAG 4 cut(s) 643, 692, 740, 759
Hsp92II CATG 4 cut(s) 59, 155, 266, 636
KspAI GTTAAC 1 cut(s) 679
Kzo9I GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
LguI GCTCTTC 1 cut(s) 574
LmnI GCTCC 2 cut(s) 244, 598
LweI GCATC 2 cut(s) 108, 448
MaeI CTAG 2 cut(s) 665, 705
MaeIII GTNAC 3 cut(s) 158, 277, 481
MalI GATC 7 cut(s) 72, 76, 186, 190, 427, 472, 548
MboI GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
MboII GAAGA 2 cut(s) 298, 591
MhlI GDGCHC 2 cut(s) 207, 586
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 4 cut(s) 250, 290, 570, 670
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 2 cut(s) 146, 390
MnlI CCTC 7 cut(s) 205, 406, 413, 612, 701, 709, 737
Mox20I TGGCCA 1 cut(s) 732
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 732
MseI TTAA 7 cut(s) 249, 302, 321, 393, 564, 669, 678
MslI CAYNNNNRTG 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 3 cut(s) 243, 467, 535
MvaI CCWGG 2 cut(s) 243, 467
MwoI GCNNNNNNNGC 3 cut(s) 34, 96, 272
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 1 cut(s) 151
NdeII GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
NlaIII CATG 4 cut(s) 59, 155, 266, 636
NlaIV GGNNCC 2 cut(s) 532, 600
NmuCI GTSAC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 417
PagI TCATGA 1 cut(s) 55
PciSI GCTCTTC 1 cut(s) 574
PfeI GAWTC 2 cut(s) 52, 614
PflFI GACNNNGTC 1 cut(s) 496
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 393
Psp6I CCWGG 2 cut(s) 241, 465
PspGI CCWGG 2 cut(s) 241, 465
PspN4I GGNNCC 2 cut(s) 532, 600
PspPI GGNCC 1 cut(s) 168
PspXI VCTCGAGB 1 cut(s) 417
PstNI CAGNNNCTG 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 496
PvuI CGATCG 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 156
SalI GTCGAC 1 cut(s) 22
SapI GCTCTTC 1 cut(s) 574
SaqAI TTAA 7 cut(s) 249, 302, 321, 393, 564, 669, 678
Sau3AI GATC 7 cut(s) 70, 74, 184, 188, 425, 470, 546
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 3 cut(s) 243, 467, 535
SduI GDGCHC 2 cut(s) 207, 586
SetI ASST 7 cut(s) 23, 173, 508, 693, 710, 720, 738
SfaNI GCATC 2 cut(s) 108, 448
Sfr274I CTCGAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 4 cut(s) 250, 290, 570, 670
SsiI CCGC 2 cut(s) 192, 476
SspMI CTAG 2 cut(s) 665, 705
StyD4I CCNGG 3 cut(s) 241, 465, 533
StyI CCWWGG 2 cut(s) 151, 171
TaaI ACNGT 1 cut(s) 164
TaqI TCGA 7 cut(s) 23, 65, 73, 129, 183, 187, 418
TasI AATT 4 cut(s) 250, 290, 570, 670
TfiI GAWTC 2 cut(s) 52, 614
Tru1I TTAA 7 cut(s) 249, 302, 321, 393, 564, 669, 678
Tru9I TTAA 7 cut(s) 249, 302, 321, 393, 564, 669, 678
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 2 cut(s) 44, 379
TspGWI ACGGA 4 cut(s) 83, 486, 591, 664
Tth111I GACNNNGTC 1 cut(s) 496
VneI GTGCAC 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 168
VspI ATTAAT 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 23
XspI CTAG 2 cut(s) 665, 705
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.