pycom12554g00020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012554
OG0002208
Physical Location & Seq
Reverse (-)
6060 .. 8590
2531 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12554g00020.1

Sequence Viewer

Length: 1464 bp
ATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCGTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGTGTAAGTATGAACAAATTCAGACTGTGAAACTTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

488

Amino Acids

53.18

Weight (kDa)

10.43

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 103, 1185
Acc36I ACCTGC 2 cut(s) 137, 1189
AccB1I GGYRCC 2 cut(s) 760, 841
AccBSI CCGCTC 1 cut(s) 1268
AccI GTMKAC 2 cut(s) 383, 444
AclI AACGTT 1 cut(s) 143
AclWI GGATC 3 cut(s) 736, 1159, 1175
AcoI YGGCCR 4 cut(s) 515, 896, 991, 1014
AcsI RAATTY 2 cut(s) 408, 1047
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 3 cut(s) 179, 1245, 1368
AfaI GTAC 4 cut(s) 66, 356, 1102, 1279
AflIII ACRYGT 2 cut(s) 81, 351
AgeI ACCGGT 2 cut(s) 447, 468
AgsI TTSAA 3 cut(s) 547, 1040, 1130
AjiI CACGTC 1 cut(s) 1216
AjnI CCWGG 3 cut(s) 502, 723, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 3 cut(s) 969, 997, 1073
AluI AGCT 3 cut(s) 969, 997, 1073
Alw21I GWGCWC 3 cut(s) 214, 468, 563
Alw26I GTCTC 3 cut(s) 100, 706, 942
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 3 cut(s) 736, 1159, 1175
Ama87I CYCGRG 4 cut(s) 258, 1198, 1257, 1271
AoxI GGCC 7 cut(s) 505, 515, 896, 991, 1014, 1222, 1230
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 132, 372, 1186
ApoI RAATTY 2 cut(s) 408, 1047
AseI ATTAAT 1 cut(s) 651
AsiGI ACCGGT 2 cut(s) 447, 468
Asp700I GAANNNNTTC 2 cut(s) 408, 1207
AspS9I GGNCC 6 cut(s) 254, 429, 450, 1134, 1222, 1230
AsuC2I CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1344
AsuHPI GGTGA 1 cut(s) 1151
AvaI CYCGRG 4 cut(s) 258, 1198, 1257, 1271
AvaII GGWCC 4 cut(s) 254, 429, 450, 1134
BaeGI GKGCMC 2 cut(s) 468, 844
BalI TGGCCA 1 cut(s) 1016
BanI GGYRCC 2 cut(s) 760, 841
BanII GRGCYC 3 cut(s) 676, 1242, 1266
BauI CACGAG 2 cut(s) 97, 213
BbsI GAAGAC 4 cut(s) 301, 685, 1028, 1369
Bbv12I GWGCWC 3 cut(s) 214, 468, 563
BbvCI CCTCAGC 1 cut(s) 953
BbvI GCAGC 3 cut(s) 119, 359, 1198
BccI CCATC 2 cut(s) 102, 1025
BceAI ACGGC 3 cut(s) 43, 364, 883
BciT130I CCWGG 3 cut(s) 504, 725, 849
BcnI CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1344
BcoDI GTCTC 3 cut(s) 100, 706, 942
BfaI CTAG 3 cut(s) 698, 966, 1053
BfoI RGCGCY 1 cut(s) 537
BfuAI ACCTGC 2 cut(s) 137, 1189
BisI GCNGC 8 cut(s) 133, 230, 373, 991, 1157, 1187, 1339, 1366
BlsI GCNGC 8 cut(s) 134, 231, 374, 992, 1158, 1188, 1340, 1367
Bme18I GGWCC 4 cut(s) 254, 429, 450, 1134
BmeT110I CYCGRG 4 cut(s) 258, 1198, 1257, 1271
BmgBI CACGTC 1 cut(s) 1216
BmgT120I GGNCC 6 cut(s) 254, 429, 450, 1134, 1222, 1230
BmiI GGNNCC 8 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313
BmsI GCATC 4 cut(s) 56, 272, 706, 1434
BpiI GAAGAC 4 cut(s) 301, 685, 1028, 1369
BplI GAGNNNNNCTC 2 cut(s) 218, 250
Bpu10I CCTNAGC 1 cut(s) 953
BpuMI CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1344
Bsa29I ATCGAT 1 cut(s) 1428
BsaHI GRCGYC 3 cut(s) 179, 1245, 1368
BsaI GGTCTC 1 cut(s) 942
BsaWI WCCGGW 3 cut(s) 447, 468, 1136
Bse118I RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
Bse3DI GCAATG 1 cut(s) 1020
BseBI CCWGG 3 cut(s) 504, 725, 849
BseCI ATCGAT 1 cut(s) 1428
BseGI GGATG 5 cut(s) 113, 287, 646, 742, 981
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 1 cut(s) 967
BsePI GCGCGC 3 cut(s) 1328, 1355, 1357
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 468, 844
BseXI GCAGC 3 cut(s) 119, 359, 1198
BsgI GTGCAG 1 cut(s) 265
Bsh1285I CGRYCG 6 cut(s) 325, 333, 361, 387, 448, 473
BshFI GGCC 7 cut(s) 507, 517, 898, 993, 1016, 1224, 1232
BshNI GGYRCC 2 cut(s) 760, 841
BshTI ACCGGT 2 cut(s) 447, 468
BshVI ATCGAT 1 cut(s) 1428
BsiEI CGRYCG 6 cut(s) 325, 333, 361, 387, 448, 473
BsiHKAI GWGCWC 3 cut(s) 214, 468, 563
BsiHKCI CYCGRG 4 cut(s) 258, 1198, 1257, 1271
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 6 cut(s) 143, 203, 465, 1021, 1073, 1232
BsmAI GTCTC 3 cut(s) 100, 706, 942
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 6 cut(s) 143, 203, 465, 1021, 1073, 1232
BsnI GGCC 7 cut(s) 507, 517, 898, 993, 1016, 1224, 1232
Bso31I GGTCTC 1 cut(s) 942
BsoBI CYCGRG 4 cut(s) 258, 1198, 1257, 1271
Bsp1286I GDGCHC 7 cut(s) 214, 468, 563, 676, 844, 1242, 1266
Bsp1407I TGTACA 1 cut(s) 1100
Bsp143I GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
BspANI GGCC 7 cut(s) 507, 517, 898, 993, 1016, 1224, 1232
BspCNI CTCAG 1 cut(s) 966
BspDI ATCGAT 1 cut(s) 1428
BspLI GGNNCC 8 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313
BspMI ACCTGC 2 cut(s) 137, 1189
BspPI GGATC 3 cut(s) 736, 1159, 1175
BspT107I GGYRCC 2 cut(s) 760, 841
BspTNI GGTCTC 1 cut(s) 942
BsrBI CCGCTC 1 cut(s) 1268
BsrDI GCAATG 1 cut(s) 1020
BsrFI RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
BsrGI TGTACA 1 cut(s) 1100
BssAI RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
BssHII GCGCGC 3 cut(s) 1328, 1355, 1357
BssMI GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
BssNI GRCGYC 3 cut(s) 179, 1245, 1368
BssSI CACGAG 2 cut(s) 97, 213
BssT1I CCWWGG 3 cut(s) 432, 636, 1307
Bst2BI CACGAG 2 cut(s) 97, 213
Bst2UI CCWGG 3 cut(s) 504, 725, 849
Bst4CI ACNGT 6 cut(s) 56, 362, 388, 418, 694, 1352
BstACI GRCGYC 3 cut(s) 179, 1245, 1368
BstAPI GCANNNNNTGC 1 cut(s) 238
BstAUI TGTACA 1 cut(s) 1100
BstDEI CTNAG 3 cut(s) 904, 953, 1001
BstDSI CCRYGG 1 cut(s) 988
BstF5I GGATG 5 cut(s) 113, 287, 646, 742, 981
BstH2I RGCGCY 1 cut(s) 537
BstKTI GATC 6 cut(s) 325, 333, 686, 731, 1154, 1170
BstMAI GTCTC 3 cut(s) 100, 706, 942
BstMBI GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
BstMCI CGRYCG 6 cut(s) 325, 333, 361, 387, 448, 473
BstMWI GCNNNNNNNGC 9 cut(s) 181, 185, 238, 298, 372, 533, 1022, 1338, 1365
BstNI CCWGG 3 cut(s) 504, 725, 849
BstSLI GKGCMC 2 cut(s) 468, 844
BstV1I GCAGC 3 cut(s) 119, 359, 1198
BstV2I GAAGAC 4 cut(s) 301, 685, 1028, 1369
Bsu15I ATCGAT 1 cut(s) 1428
BsuRI GGCC 7 cut(s) 507, 517, 898, 993, 1016, 1224, 1232
BsuTUI ATCGAT 1 cut(s) 1428
BtgI CCRYGG 1 cut(s) 988
BtrI CACGTC 1 cut(s) 1216
BtsCI GGATG 5 cut(s) 113, 287, 646, 742, 981
BveI ACCTGC 2 cut(s) 137, 1189
Cfr10I RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
Cfr13I GGNCC 6 cut(s) 254, 429, 450, 1134, 1222, 1230
Cfr42I CCGCGG 1 cut(s) 991
Cfr9I CCCGGG 2 cut(s) 1257, 1271
ClaI ATCGAT 1 cut(s) 1428
CpoI CGGWCCG 1 cut(s) 450
CseI GACGC 8 cut(s) 72, 89, 168, 359, 1170, 1234, 1285, 1357
Csp6I GTAC 4 cut(s) 65, 355, 1101, 1278
CspAI ACCGGT 2 cut(s) 447, 468
CspI CGGWCCG 1 cut(s) 450
CviAII CATG 2 cut(s) 594, 894
CviQI GTAC 4 cut(s) 65, 355, 1101, 1278
DdeI CTNAG 3 cut(s) 904, 953, 1001
DpnI GATC 6 cut(s) 324, 332, 685, 730, 1153, 1169
DpnII GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
EaeI YGGCCR 4 cut(s) 515, 896, 991, 1014
EciI GGCGGA 3 cut(s) 442, 744, 749
Eco130I CCWWGG 3 cut(s) 432, 636, 1307
Eco24I GRGCYC 3 cut(s) 676, 1242, 1266
Eco31I GGTCTC 1 cut(s) 942
Eco32I GATATC 1 cut(s) 1412
Eco47I GGWCC 4 cut(s) 254, 429, 450, 1134
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 4 cut(s) 258, 1198, 1257, 1271
EcoRI GAATTC 1 cut(s) 1047
EcoRII CCWGG 3 cut(s) 502, 723, 847
EcoRV GATATC 1 cut(s) 1412
EcoT14I CCWWGG 3 cut(s) 432, 636, 1307
EcoT38I GRGCYC 3 cut(s) 676, 1242, 1266
ErhI CCWWGG 3 cut(s) 432, 636, 1307
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 2 cut(s) 597, 897
FaqI GGGAC 6 cut(s) 143, 203, 465, 1021, 1073, 1232
FatI CATG 2 cut(s) 593, 893
FauI CCCGC 8 cut(s) 77, 181, 377, 1157, 1221, 1273, 1343, 1354
FblI GTMKAC 2 cut(s) 383, 444
Fnu4HI GCNGC 8 cut(s) 133, 230, 373, 991, 1157, 1187, 1339, 1366
FokI GGATG 5 cut(s) 120, 294, 653, 749, 968
FriOI GRGCYC 3 cut(s) 676, 1242, 1266
Fsp4HI GCNGC 8 cut(s) 133, 230, 373, 991, 1157, 1187, 1339, 1366
FspBI CTAG 3 cut(s) 698, 966, 1053
FspI TGCGCA 2 cut(s) 103, 1185
GluI GCNGC 8 cut(s) 133, 230, 373, 991, 1157, 1187, 1339, 1366
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 7 cut(s) 507, 517, 898, 993, 1016, 1224, 1232
HgaI GACGC 8 cut(s) 72, 89, 168, 359, 1170, 1234, 1285, 1357
Hin1I GRCGYC 3 cut(s) 179, 1245, 1368
Hin1II CATG 2 cut(s) 597, 897
HincII GTYRAC 4 cut(s) 384, 445, 940, 1081
HindII GTYRAC 4 cut(s) 384, 445, 940, 1081
HinfI GANTC 5 cut(s) 90, 751, 875, 1064, 1396
HpaI GTTAAC 1 cut(s) 940
HphI GGTGA 1 cut(s) 1151
Hpy166II GTNNAC 6 cut(s) 384, 445, 466, 940, 1081, 1103
Hpy188I TCNGA 7 cut(s) 281, 322, 330, 414, 582, 688, 1151
Hpy188III TCNNGA 4 cut(s) 87, 313, 656, 872
Hpy8I GTNNAC 6 cut(s) 384, 445, 466, 940, 1081, 1103
HpyAV CCTTC 5 cut(s) 131, 289, 493, 831, 1243
HpyCH4III ACNGT 6 cut(s) 56, 362, 388, 418, 694, 1352
HpyCH4IV ACGT 4 cut(s) 143, 1086, 1215, 1438
HpyCH4V TGCA 4 cut(s) 246, 466, 893, 1025
HpyF10VI GCNNNNNNNGC 9 cut(s) 181, 185, 238, 298, 372, 533, 1022, 1338, 1365
HpyF3I CTNAG 3 cut(s) 904, 953, 1001
HpySE526I ACGT 4 cut(s) 143, 1086, 1215, 1438
Hsp92I GRCGYC 3 cut(s) 179, 1245, 1368
Hsp92II CATG 2 cut(s) 597, 897
KroI GCCGGC 5 cut(s) 175, 184, 489, 757, 1224
KroNI GCCGGC 5 cut(s) 177, 186, 491, 759, 1226
KspAI GTTAAC 1 cut(s) 940
KspI CCGCGG 1 cut(s) 991
Kzo9I GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
LmnI GCTCC 8 cut(s) 39, 505, 856, 914, 1122, 1261, 1273, 1337
Lsp1109I GCAGC 3 cut(s) 119, 359, 1198
LweI GCATC 4 cut(s) 56, 272, 706, 1434
MaeI CTAG 3 cut(s) 698, 966, 1053
MaeII ACGT 4 cut(s) 143, 1086, 1215, 1438
MaeIII GTNAC 2 cut(s) 538, 739
MalI GATC 6 cut(s) 324, 332, 685, 730, 1153, 1169
MauBI CGCGCGCG 1 cut(s) 1357
MbiI CCGCTC 1 cut(s) 1268
MboI GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
MboII GAAGA 6 cut(s) 301, 559, 690, 1028, 1369, 1445
MfeI CAATTG 1 cut(s) 1026
MhlI GDGCHC 7 cut(s) 214, 468, 563, 676, 844, 1242, 1266
MlsI TGGCCA 1 cut(s) 1016
MluCI AATT 8 cut(s) 10, 408, 511, 551, 828, 931, 1026, 1047
MluI ACGCGT 2 cut(s) 81, 351
MluNI TGGCCA 1 cut(s) 1016
MlyI GAGTC 3 cut(s) 745, 1073, 1390
Mox20I TGGCCA 1 cut(s) 1016
MreI CGCCGGCG 1 cut(s) 184
MroNI GCCGGC 5 cut(s) 175, 184, 489, 757, 1224
MroXI GAANNNNTTC 2 cut(s) 408, 1207
MscI TGGCCA 1 cut(s) 1016
MseI TTAA 5 cut(s) 510, 651, 822, 930, 939
MslI CAYNNNNRTG 1 cut(s) 592
Msp20I TGGCCA 1 cut(s) 1016
MspA1I CMGCKG 2 cut(s) 372, 990
MunI CAATTG 1 cut(s) 1026
MvaI CCWGG 3 cut(s) 504, 725, 849
MwoI GCNNNNNNNGC 9 cut(s) 181, 185, 238, 298, 372, 533, 1022, 1338, 1365
NaeI GCCGGC 5 cut(s) 177, 186, 491, 759, 1226
NciI CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1344
NdeII GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
NgoMIV GCCGGC 5 cut(s) 175, 184, 489, 757, 1224
NlaIII CATG 2 cut(s) 597, 897
NlaIV GGNNCC 8 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313
NmeAIII GCCGAG 3 cut(s) 1221, 1380, 1395
NsbI TGCGCA 2 cut(s) 103, 1185
PauI GCGCGC 3 cut(s) 1328, 1355, 1357
PcsI WCGNNNNNNNCGW 4 cut(s) 350, 354, 1121, 1242
PdiI GCCGGC 5 cut(s) 177, 186, 491, 759, 1226
PdmI GAANNNNTTC 2 cut(s) 408, 1207
PfeI GAWTC 2 cut(s) 90, 875
Pfl23II CGTACG 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 1085
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 447, 468
PkrI GCNGC 8 cut(s) 134, 231, 374, 992, 1158, 1188, 1340, 1367
Ple19I CGATCG 2 cut(s) 325, 333
PleI GAGTC 3 cut(s) 745, 1072, 1390
PpsI GAGTC 3 cut(s) 745, 1072, 1390
PshBI ATTAAT 1 cut(s) 651
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 3 cut(s) 502, 723, 847
PspGI CCWGG 3 cut(s) 502, 723, 847
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 8 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313
PspPI GGNCC 6 cut(s) 254, 429, 450, 1134, 1222, 1230
PsyI GACNNNGTC 1 cut(s) 1085
PteI GCGCGC 3 cut(s) 1328, 1355, 1357
PvuI CGATCG 2 cut(s) 325, 333
RsaI GTAC 4 cut(s) 66, 356, 1102, 1279
RsaNI GTAC 4 cut(s) 65, 355, 1101, 1278
RseI CAYNNNNRTG 1 cut(s) 592
Rsr2I CGGWCCG 1 cut(s) 450
RsrII CGGWCCG 1 cut(s) 450
SacII CCGCGG 1 cut(s) 991
SalI GTCGAC 2 cut(s) 382, 443
SaqAI TTAA 5 cut(s) 510, 651, 822, 930, 939
SatI GCNGC 8 cut(s) 133, 230, 373, 991, 1157, 1187, 1339, 1366
Sau3AI GATC 6 cut(s) 322, 330, 683, 728, 1151, 1167
Sau96I GGNCC 6 cut(s) 254, 429, 450, 1134, 1222, 1230
SchI GAGTC 3 cut(s) 745, 1073, 1390
SduI GDGCHC 7 cut(s) 214, 468, 563, 676, 844, 1242, 1266
SfaNI GCATC 4 cut(s) 56, 272, 706, 1434
Sfr303I CCGCGG 1 cut(s) 991
SgrAI CRCCGGYG 2 cut(s) 184, 1290
SgrBI CCGCGG 1 cut(s) 991
SinI GGWCC 4 cut(s) 254, 429, 450, 1134
SmaI CCCGGG 2 cut(s) 1259, 1273
SmiMI CAYNNNNRTG 1 cut(s) 592
Sse9I AATT 8 cut(s) 10, 408, 511, 551, 828, 931, 1026, 1047
SspMI CTAG 3 cut(s) 698, 966, 1053
StyI CCWWGG 3 cut(s) 432, 636, 1307
TaaI ACNGT 6 cut(s) 56, 362, 388, 418, 694, 1352
TaiI ACGT 4 cut(s) 146, 1089, 1218, 1441
TaqII GACCGA 1 cut(s) 242
TasI AATT 8 cut(s) 10, 408, 511, 551, 828, 931, 1026, 1047
TatI WGTACW 1 cut(s) 1100
TauI GCSGC 5 cut(s) 232, 993, 1159, 1341, 1368
TfiI GAWTC 2 cut(s) 90, 875
Tru1I TTAA 5 cut(s) 510, 651, 822, 930, 939
Tru9I TTAA 5 cut(s) 510, 651, 822, 930, 939
TseI GCWGC 3 cut(s) 132, 372, 1186
TspDTI ATGAA 4 cut(s) 64, 417, 637, 1446
TspGWI ACGGA 5 cut(s) 26, 256, 849, 925, 1422
TspMI CCCGGG 2 cut(s) 1257, 1271
Tth111I GACNNNGTC 1 cut(s) 1085
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 4 cut(s) 254, 429, 450, 1134
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 2 cut(s) 408, 1047
XmaI CCCGGG 2 cut(s) 1257, 1271
XmiI GTMKAC 2 cut(s) 383, 444
XmnI GAANNNNTTC 2 cut(s) 408, 1207
XspI CTAG 3 cut(s) 698, 966, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.