pycom280g00100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000280
OG0002208
Physical Location & Seq
Reverse (-)
32956 .. 34519
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom280g00100.2

Sequence Viewer

Length: 987 bp
ATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACGCCGACGTCGAAGAGGAATTTATAGTTTGTTTGATGGTATCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACAGCCATCGTGCTGGCGACGCATCATTCAAATATCTCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGAGTATGGTCGCAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

36.41

Weight (kDa)

10.36

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 375
AatII GACGTC 1 cut(s) 379
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 931
AccI GTMKAC 3 cut(s) 341, 500, 615
AccII CGCG 6 cut(s) 83, 311, 324, 365, 367, 505
AclI AACGTT 1 cut(s) 143
AclWI GGATC 2 cut(s) 555, 907
AcoI YGGCCR 1 cut(s) 686
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 2 cut(s) 370, 376
AfaI GTAC 2 cut(s) 66, 314
AfiI CCNNNNNNNGG 6 cut(s) 43, 178, 469, 609, 660, 901
AflIII ACRYGT 2 cut(s) 81, 309
AgeI ACCGGT 2 cut(s) 618, 639
AgsI TTSAA 2 cut(s) 582, 718
AjnI CCWGG 2 cut(s) 673, 894
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AluBI AGCT 1 cut(s) 443
AluI AGCT 1 cut(s) 443
Alw21I GWGCWC 2 cut(s) 639, 734
Alw26I GTCTC 3 cut(s) 100, 365, 877
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 2 cut(s) 555, 907
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 2 cut(s) 676, 686
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 2 cut(s) 132, 330
ApoI RAATTY 1 cut(s) 387
AseI ATTAAT 1 cut(s) 822
AsiGI ACCGGT 2 cut(s) 618, 639
AspLEI GCGC 4 cut(s) 104, 326, 367, 707
AspS9I GGNCC 3 cut(s) 212, 600, 621
AsuC2I CCSGG 3 cut(s) 179, 690, 964
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 3 cut(s) 212, 600, 621
BaeGI GKGCMC 1 cut(s) 639
BanI GGYRCC 1 cut(s) 931
BanII GRGCYC 2 cut(s) 511, 847
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 259, 856
Bbv12I GWGCWC 2 cut(s) 639, 734
BbvI GCAGC 2 cut(s) 119, 317
BccI CCATC 3 cut(s) 102, 398, 566
BceAI ACGGC 2 cut(s) 43, 322
BcgI CGANNNNNNTGC 2 cut(s) 541, 575
BciT130I CCWGG 2 cut(s) 675, 896
BcnI CCSGG 3 cut(s) 179, 690, 964
BcoDI GTCTC 3 cut(s) 100, 365, 877
BfaI CTAG 2 cut(s) 438, 869
BfoI RGCGCY 1 cut(s) 708
BfuAI ACCTGC 1 cut(s) 137
BisI GCNGC 3 cut(s) 133, 188, 331
BlsI GCNGC 3 cut(s) 134, 189, 332
Bme1390I CCNGG 5 cut(s) 179, 675, 690, 896, 964
Bme18I GGWCC 3 cut(s) 212, 600, 621
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 3 cut(s) 212, 600, 621
BmiI GGNNCC 3 cut(s) 35, 933, 961
BmrFI CCNGG 5 cut(s) 179, 675, 690, 896, 964
BmsI GCATC 4 cut(s) 56, 230, 583, 877
BpiI GAAGAC 2 cut(s) 259, 856
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuMI CCSGG 3 cut(s) 179, 690, 964
BsaAI YACGTR 1 cut(s) 450
BsaHI GRCGYC 2 cut(s) 370, 376
BsaJI CCNNGG 5 cut(s) 215, 603, 807, 895, 963
BsaWI WCCGGW 2 cut(s) 618, 639
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 178, 469, 609, 660, 901
Bse118I RCCGGY 4 cut(s) 618, 639, 660, 928
BseBI CCWGG 2 cut(s) 675, 896
BseDI CCNNGG 5 cut(s) 215, 603, 807, 895, 963
BseGI GGATG 4 cut(s) 113, 245, 817, 913
BseLI CCNNNNNNNGG 6 cut(s) 43, 178, 469, 609, 660, 901
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 639
BseXI GCAGC 2 cut(s) 119, 317
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 6 cut(s) 83, 311, 324, 365, 367, 505
Bsh1285I CGRYCG 6 cut(s) 283, 291, 319, 345, 619, 644
BshFI GGCC 2 cut(s) 678, 688
BshNI GGYRCC 1 cut(s) 931
BshTI ACCGGT 2 cut(s) 618, 639
BsiEI CGRYCG 6 cut(s) 283, 291, 319, 345, 619, 644
BsiHKAI GWGCWC 2 cut(s) 639, 734
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 8 cut(s) 179, 619, 640, 661, 689, 702, 929, 963
BsiWI CGTACG 1 cut(s) 312
BslFI GGGAC 4 cut(s) 143, 161, 490, 636
BslI CCNNNNNNNGG 6 cut(s) 43, 178, 469, 609, 660, 901
BsmAI GTCTC 3 cut(s) 100, 365, 877
BsmBI CGTCTC 2 cut(s) 100, 365
BsmFI GGGAC 4 cut(s) 143, 161, 490, 636
BsnI GGCC 2 cut(s) 678, 688
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 4 cut(s) 511, 639, 734, 847
Bsp143I GATC 5 cut(s) 280, 288, 547, 854, 899
BspANI GGCC 2 cut(s) 678, 688
BspFNI CGCG 6 cut(s) 83, 311, 324, 365, 367, 505
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 3 cut(s) 35, 933, 961
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 2 cut(s) 555, 907
BspT107I GGYRCC 1 cut(s) 931
BsrFI RCCGGY 4 cut(s) 618, 639, 660, 928
BssAI RCCGGY 4 cut(s) 618, 639, 660, 928
BssECI CCNNGG 5 cut(s) 215, 603, 807, 895, 963
BssMI GATC 5 cut(s) 280, 288, 547, 854, 899
BssNI GRCGYC 2 cut(s) 370, 376
BssSI CACGAG 1 cut(s) 97
BssT1I CCWWGG 2 cut(s) 603, 807
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 2 cut(s) 675, 896
Bst4CI ACNGT 5 cut(s) 56, 320, 346, 428, 865
Bst6I CTCTTC 1 cut(s) 376
BstACI GRCGYC 2 cut(s) 370, 376
BstAPI GCANNNNNTGC 1 cut(s) 196
BstBAI YACGTR 1 cut(s) 450
BstC8I GCNNGC 8 cut(s) 32, 328, 335, 507, 567, 662, 741, 930
BstF5I GGATG 4 cut(s) 113, 245, 817, 913
BstFNI CGCG 6 cut(s) 83, 311, 324, 365, 367, 505
BstH2I RGCGCY 1 cut(s) 708
BstHHI GCGC 4 cut(s) 104, 326, 367, 707
BstKTI GATC 5 cut(s) 283, 291, 550, 857, 902
BstMAI GTCTC 3 cut(s) 100, 365, 877
BstMBI GATC 5 cut(s) 280, 288, 547, 854, 899
BstMCI CGRYCG 6 cut(s) 283, 291, 319, 345, 619, 644
BstMWI GCNNNNNNNGC 6 cut(s) 196, 256, 330, 511, 571, 704
BstNI CCWGG 2 cut(s) 675, 896
BstSCI CCNGG 5 cut(s) 177, 673, 688, 894, 962
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 6 cut(s) 83, 311, 324, 365, 367, 505
BstV1I GCAGC 2 cut(s) 119, 317
BstV2I GAAGAC 2 cut(s) 259, 856
BstXI CCANNNNNNTGG 1 cut(s) 565
BsuRI GGCC 2 cut(s) 678, 688
BtsCI GGATG 4 cut(s) 113, 245, 817, 913
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 8 cut(s) 32, 328, 335, 507, 567, 662, 741, 930
CciI TCATGA 1 cut(s) 532
CfoI GCGC 4 cut(s) 104, 326, 367, 707
Cfr10I RCCGGY 4 cut(s) 618, 639, 660, 928
Cfr13I GGNCC 3 cut(s) 212, 600, 621
CpoI CGGWCCG 1 cut(s) 621
CseI GACGC 7 cut(s) 72, 89, 317, 378, 487, 511, 580
Csp6I GTAC 2 cut(s) 65, 313
CspAI ACCGGT 2 cut(s) 618, 639
CspI CGGWCCG 1 cut(s) 621
CviAII CATG 2 cut(s) 533, 765
CviQI GTAC 2 cut(s) 65, 313
DpnI GATC 5 cut(s) 282, 290, 549, 856, 901
DpnII GATC 5 cut(s) 280, 288, 547, 854, 899
DrdI GACNNNNNNGTC 1 cut(s) 375
DseDI GACNNNNNNGTC 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 686
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
EciI GGCGGA 3 cut(s) 613, 915, 920
Eco130I CCWWGG 2 cut(s) 603, 807
Eco24I GRGCYC 2 cut(s) 511, 847
Eco47I GGWCC 3 cut(s) 212, 600, 621
Eco57I CTGAAG 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 216
EcoRII CCWGG 2 cut(s) 673, 894
EcoT14I CCWWGG 2 cut(s) 603, 807
EcoT38I GRGCYC 2 cut(s) 511, 847
ErhI CCWWGG 2 cut(s) 603, 807
Esp3I CGTCTC 2 cut(s) 100, 365
FaeI CATG 2 cut(s) 536, 768
FaiI YATR 7 cut(s) 393, 534, 766, 779, 881, 939, 973
FaqI GGGAC 4 cut(s) 143, 161, 490, 636
FatI CATG 2 cut(s) 532, 764
FauI CCCGC 3 cut(s) 77, 335, 498
FblI GTMKAC 3 cut(s) 341, 500, 615
Fnu4HI GCNGC 3 cut(s) 133, 188, 331
FokI GGATG 4 cut(s) 120, 252, 824, 920
FriOI GRGCYC 2 cut(s) 511, 847
Fsp4HI GCNGC 3 cut(s) 133, 188, 331
FspBI CTAG 2 cut(s) 438, 869
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 4 cut(s) 103, 325, 366, 706
GluI GCNGC 3 cut(s) 133, 188, 331
HaeII RGCGCY 1 cut(s) 708
HaeIII GGCC 2 cut(s) 678, 688
HapII CCGG 8 cut(s) 179, 619, 640, 661, 689, 702, 929, 963
HgaI GACGC 7 cut(s) 72, 89, 317, 378, 487, 511, 580
HhaI GCGC 4 cut(s) 104, 326, 367, 707
Hin1I GRCGYC 2 cut(s) 370, 376
Hin1II CATG 2 cut(s) 536, 768
Hin6I GCGC 4 cut(s) 102, 324, 365, 705
HinP1I GCGC 4 cut(s) 102, 324, 365, 705
HincII GTYRAC 3 cut(s) 342, 501, 616
HindII GTYRAC 3 cut(s) 342, 501, 616
HinfI GANTC 3 cut(s) 90, 529, 922
HpaII CCGG 8 cut(s) 179, 619, 640, 661, 689, 702, 929, 963
Hpy166II GTNNAC 4 cut(s) 342, 501, 616, 637
Hpy188I TCNGA 7 cut(s) 239, 280, 288, 421, 525, 753, 859
Hpy188III TCNNGA 6 cut(s) 87, 271, 438, 470, 533, 827
Hpy8I GTNNAC 4 cut(s) 342, 501, 616, 637
HpyAV CCTTC 4 cut(s) 131, 247, 467, 664
HpyCH4III ACNGT 5 cut(s) 56, 320, 346, 428, 865
HpyCH4IV ACGT 3 cut(s) 143, 376, 449
HpyCH4V TGCA 3 cut(s) 204, 453, 637
HpyF10VI GCNNNNNNNGC 6 cut(s) 196, 256, 330, 511, 571, 704
HpySE526I ACGT 3 cut(s) 143, 376, 449
Hsp92I GRCGYC 2 cut(s) 370, 376
Hsp92II CATG 2 cut(s) 536, 768
HspAI GCGC 4 cut(s) 102, 324, 365, 705
KroI GCCGGC 2 cut(s) 660, 928
KroNI GCCGGC 2 cut(s) 662, 930
Kzo9I GATC 5 cut(s) 280, 288, 547, 854, 899
LmnI GCTCC 2 cut(s) 39, 676
Lsp1109I GCAGC 2 cut(s) 119, 317
LweI GCATC 4 cut(s) 56, 230, 583, 877
MaeI CTAG 2 cut(s) 438, 869
MaeII ACGT 3 cut(s) 143, 376, 449
MaeIII GTNAC 2 cut(s) 709, 910
MalI GATC 5 cut(s) 282, 290, 549, 856, 901
MboI GATC 5 cut(s) 280, 288, 547, 854, 899
MboII GAAGA 4 cut(s) 259, 393, 730, 861
MhlI GDGCHC 4 cut(s) 511, 639, 734, 847
MluCI AATT 5 cut(s) 10, 387, 433, 682, 722
MluI ACGCGT 2 cut(s) 81, 309
MlyI GAGTC 1 cut(s) 916
MmeI TCCRAC 1 cut(s) 578
MnlI CCTC 8 cut(s) 159, 176, 179, 225, 286, 377, 637, 709
MroNI GCCGGC 2 cut(s) 660, 928
MseI TTAA 2 cut(s) 681, 822
MslI CAYNNNNRTG 1 cut(s) 763
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 8 cut(s) 179, 619, 640, 661, 689, 702, 929, 963
MspR9I CCNGG 5 cut(s) 179, 675, 690, 896, 964
MvaI CCWGG 2 cut(s) 675, 896
MvnI CGCG 6 cut(s) 83, 311, 324, 365, 367, 505
MwoI GCNNNNNNNGC 6 cut(s) 196, 256, 330, 511, 571, 704
NaeI GCCGGC 2 cut(s) 662, 930
NciI CCSGG 3 cut(s) 179, 690, 964
NdeII GATC 5 cut(s) 280, 288, 547, 854, 899
NgoMIV GCCGGC 2 cut(s) 660, 928
NlaIII CATG 2 cut(s) 536, 768
NlaIV GGNNCC 3 cut(s) 35, 933, 961
NsbI TGCGCA 1 cut(s) 103
PagI TCATGA 1 cut(s) 532
PcsI WCGNNNNNNNCGW 3 cut(s) 308, 312, 376
PdiI GCCGGC 2 cut(s) 662, 930
PfeI GAWTC 2 cut(s) 90, 529
Pfl23II CGTACG 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 177
PinAI ACCGGT 2 cut(s) 618, 639
PkrI GCNGC 3 cut(s) 134, 189, 332
Ple19I CGATCG 2 cut(s) 283, 291
PleI GAGTC 1 cut(s) 916
PpsI GAGTC 1 cut(s) 916
Ppu21I YACGTR 1 cut(s) 450
PshBI ATTAAT 1 cut(s) 822
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 673, 894
PspGI CCWGG 2 cut(s) 673, 894
PspLI CGTACG 1 cut(s) 312
PspN4I GGNNCC 3 cut(s) 35, 933, 961
PspPI GGNCC 3 cut(s) 212, 600, 621
PvuI CGATCG 2 cut(s) 283, 291
RsaI GTAC 2 cut(s) 66, 314
RsaNI GTAC 2 cut(s) 65, 313
RseI CAYNNNNRTG 1 cut(s) 763
Rsr2I CGGWCCG 1 cut(s) 621
RsrII CGGWCCG 1 cut(s) 621
SalI GTCGAC 3 cut(s) 340, 499, 614
SaqAI TTAA 2 cut(s) 681, 822
SatI GCNGC 3 cut(s) 133, 188, 331
Sau3AI GATC 5 cut(s) 280, 288, 547, 854, 899
Sau96I GGNCC 3 cut(s) 212, 600, 621
SchI GAGTC 1 cut(s) 916
ScrFI CCNGG 5 cut(s) 179, 675, 690, 896, 964
SduI GDGCHC 4 cut(s) 511, 639, 734, 847
SfaNI GCATC 4 cut(s) 56, 230, 583, 877
SinI GGWCC 3 cut(s) 212, 600, 621
SmiMI CAYNNNNRTG 1 cut(s) 763
Sse9I AATT 5 cut(s) 10, 387, 433, 682, 722
SspMI CTAG 2 cut(s) 438, 869
StyD4I CCNGG 5 cut(s) 177, 673, 688, 894, 962
StyI CCWWGG 2 cut(s) 603, 807
TaaI ACNGT 5 cut(s) 56, 320, 346, 428, 865
TaiI ACGT 3 cut(s) 146, 379, 452
TaqII GACCGA 1 cut(s) 200
TasI AATT 5 cut(s) 10, 387, 433, 682, 722
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 2 cut(s) 90, 529
Tru1I TTAA 2 cut(s) 681, 822
Tru9I TTAA 2 cut(s) 681, 822
TseI GCWGC 2 cut(s) 132, 330
TspDTI ATGAA 3 cut(s) 64, 521, 808
TspGWI ACGGA 4 cut(s) 26, 214, 347, 560
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 3 cut(s) 212, 600, 621
VspI ATTAAT 1 cut(s) 822
XapI RAATTY 1 cut(s) 387
XbaI TCTAGA 1 cut(s) 437
XmiI GTMKAC 3 cut(s) 341, 500, 615
XspI CTAG 2 cut(s) 438, 869
ZraI GACGTC 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.