pycom280g00090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000280
OG0002208
Physical Location & Seq
Reverse (-)
31575 .. 32826
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom280g00090.1

Sequence Viewer

Length: 939 bp
ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCGCGCCTCCCCCGGGAGCGTCGGGGGGCGGACGTTGGCCTCCCGTGCGTCCCCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCGAACCTCGGTTGCCCGTTGTGCGCCTTCGTCGCGCCCGAGCGGCCCGCGACGCTCCCCGCTCCGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

33.92

Weight (kDa)

11.67

Isoelectric Point (pI)

69.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 306
Acc36I ACCTGC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 580
AccBSI CCGCTC 3 cut(s) 389, 779, 798
AclWI GGATC 2 cut(s) 280, 296
AcoI YGGCCR 4 cut(s) 17, 112, 135, 807
AcsI RAATTY 2 cut(s) 168, 853
AcyI GRCGYC 3 cut(s) 366, 489, 604
AfaI GTAC 2 cut(s) 223, 400
AgsI TTSAA 3 cut(s) 161, 251, 819
AhdI GACNNNNNGTC 1 cut(s) 830
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 598, 828
AluBI AGCT 5 cut(s) 90, 118, 194, 814, 935
AluI AGCT 5 cut(s) 90, 118, 194, 814, 935
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 2 cut(s) 280, 296
Ama87I CYCGRG 5 cut(s) 319, 378, 392, 634, 774
AoxI GGCC 8 cut(s) 17, 112, 135, 343, 351, 659, 780, 807
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 2 cut(s) 168, 853
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
Asp700I GAANNNNTTC 1 cut(s) 328
AspS9I GGNCC 5 cut(s) 255, 343, 351, 701, 781
AsuC2I CCSGG 9 cut(s) 379, 380, 393, 394, 465, 635, 636, 699, 922
AsuHPI GGTGA 1 cut(s) 272
AvaI CYCGRG 5 cut(s) 319, 378, 392, 634, 774
AvaII GGWCC 2 cut(s) 255, 701
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 137
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 2 cut(s) 363, 387
BbsI GAAGAC 2 cut(s) 149, 490
BbvCI CCTCAGC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 319
BccI CCATC 1 cut(s) 146
BceAI ACGGC 3 cut(s) 4, 598, 925
BciT130I CCWGG 2 cut(s) 600, 830
BcnI CCSGG 9 cut(s) 379, 380, 393, 394, 465, 635, 636, 699, 922
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 3 cut(s) 87, 174, 903
BfuAI ACCTGC 1 cut(s) 310
BglI GCCNNNNNGGC 1 cut(s) 779
BisI GCNGC 6 cut(s) 112, 278, 308, 460, 487, 780
BlsI GCNGC 6 cut(s) 113, 279, 309, 461, 488, 781
Bme18I GGWCC 2 cut(s) 255, 701
BmeRI GACNNNNNGTC 1 cut(s) 830
BmeT110I CYCGRG 5 cut(s) 319, 378, 392, 634, 774
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 5 cut(s) 255, 343, 351, 701, 781
BmiI GGNNCC 5 cut(s) 344, 384, 434, 582, 702
BmsI GCATC 1 cut(s) 555
BpiI GAAGAC 2 cut(s) 149, 490
Bpu10I CCTNAGC 1 cut(s) 74
BpuMI CCSGG 9 cut(s) 379, 380, 393, 394, 465, 635, 636, 699, 922
Bsa29I ATCGAT 1 cut(s) 549
BsaHI GRCGYC 3 cut(s) 366, 489, 604
BsaI GGTCTC 1 cut(s) 63
BsaWI WCCGGW 1 cut(s) 257
Bse118I RCCGGY 2 cut(s) 345, 411
Bse3DI GCAATG 1 cut(s) 141
BseBI CCWGG 2 cut(s) 600, 830
BseCI ATCGAT 1 cut(s) 549
BseGI GGATG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 88
BsePI GCGCGC 3 cut(s) 449, 476, 478
BseSI GKGCMC 1 cut(s) 592
BseX3I CGGCCG 1 cut(s) 807
BseXI GCAGC 1 cut(s) 319
BseYI CCCAGC 1 cut(s) 931
Bsh1285I CGRYCG 1 cut(s) 810
BshFI GGCC 8 cut(s) 19, 114, 137, 345, 353, 661, 782, 809
BshNI GGYRCC 1 cut(s) 580
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 810
BsiHKCI CYCGRG 5 cut(s) 319, 378, 392, 634, 774
BsiSI CCGG 9 cut(s) 258, 346, 379, 393, 412, 465, 635, 698, 921
BslFI GGGAC 4 cut(s) 142, 194, 353, 657
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 4 cut(s) 142, 194, 353, 657
BsnI GGCC 8 cut(s) 19, 114, 137, 345, 353, 661, 782, 809
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 5 cut(s) 319, 378, 392, 634, 774
Bsp1286I GDGCHC 3 cut(s) 363, 387, 592
Bsp1407I TGTACA 1 cut(s) 221
Bsp143I GATC 2 cut(s) 272, 288
BspANI GGCC 8 cut(s) 19, 114, 137, 345, 353, 661, 782, 809
BspCNI CTCAG 1 cut(s) 87
BspDI ATCGAT 1 cut(s) 549
BspLI GGNNCC 5 cut(s) 344, 384, 434, 582, 702
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 2 cut(s) 280, 296
BspT107I GGYRCC 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 63
BsrBI CCGCTC 3 cut(s) 389, 779, 798
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 2 cut(s) 345, 411
BsrGI TGTACA 1 cut(s) 221
BssAI RCCGGY 2 cut(s) 345, 411
BssHII GCGCGC 3 cut(s) 449, 476, 478
BssMI GATC 2 cut(s) 272, 288
BssNI GRCGYC 3 cut(s) 366, 489, 604
BssT1I CCWWGG 1 cut(s) 428
Bst2UI CCWGG 2 cut(s) 600, 830
Bst4CI ACNGT 1 cut(s) 473
BstACI GRCGYC 3 cut(s) 366, 489, 604
BstAUI TGTACA 1 cut(s) 221
BstDEI CTNAG 3 cut(s) 25, 74, 122
BstDSI CCRYGG 1 cut(s) 109
BstEII GGTNACC 1 cut(s) 842
BstF5I GGATG 1 cut(s) 102
BstKTI GATC 2 cut(s) 275, 291
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 2 cut(s) 272, 288
BstMCI CGRYCG 1 cut(s) 810
BstNI CCWGG 2 cut(s) 600, 830
BstPI GGTNACC 1 cut(s) 842
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 2 cut(s) 149, 490
BstZI CGGCCG 1 cut(s) 807
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 8 cut(s) 19, 114, 137, 345, 353, 661, 782, 809
BsuTUI ATCGAT 1 cut(s) 549
BtgI CCRYGG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 310
Cfr10I RCCGGY 2 cut(s) 345, 411
Cfr13I GGNCC 5 cut(s) 255, 343, 351, 701, 781
Cfr42I CCGCGG 1 cut(s) 112
Cfr9I CCCGGG 3 cut(s) 378, 392, 634
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 8 cut(s) 291, 355, 406, 478, 593, 630, 659, 797
Csp6I GTAC 2 cut(s) 222, 399
CviAII CATG 1 cut(s) 15
CviQI GTAC 2 cut(s) 222, 399
DdeI CTNAG 3 cut(s) 25, 74, 122
DpnI GATC 2 cut(s) 274, 290
DpnII GATC 2 cut(s) 272, 288
DriI GACNNNNNGTC 1 cut(s) 830
EaeI YGGCCR 4 cut(s) 17, 112, 135, 807
EagI CGGCCG 1 cut(s) 807
Eam1105I GACNNNNNGTC 1 cut(s) 830
EciI GGCGGA 1 cut(s) 666
EclXI CGGCCG 1 cut(s) 807
Eco130I CCWWGG 1 cut(s) 428
Eco24I GRGCYC 2 cut(s) 363, 387
Eco31I GGTCTC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 533
Eco47I GGWCC 2 cut(s) 255, 701
Eco52I CGGCCG 1 cut(s) 807
Eco88I CYCGRG 5 cut(s) 319, 378, 392, 634, 774
Eco91I GGTNACC 1 cut(s) 842
EcoO109I RGGNCCY 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 842
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 598, 828
EcoRV GATATC 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 428
EcoT38I GRGCYC 2 cut(s) 363, 387
ErhI CCWWGG 1 cut(s) 428
FaeI CATG 1 cut(s) 18
FaiI YATR 4 cut(s) 16, 93, 134, 863
FaqI GGGAC 4 cut(s) 142, 194, 353, 657
FatI CATG 1 cut(s) 14
Fnu4HI GCNGC 6 cut(s) 112, 278, 308, 460, 487, 780
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 2 cut(s) 363, 387
Fsp4HI GCNGC 6 cut(s) 112, 278, 308, 460, 487, 780
FspBI CTAG 3 cut(s) 87, 174, 903
FspI TGCGCA 1 cut(s) 306
GluI GCNGC 6 cut(s) 112, 278, 308, 460, 487, 780
GsaI CCCAGC 1 cut(s) 935
HaeIII GGCC 8 cut(s) 19, 114, 137, 345, 353, 661, 782, 809
HapII CCGG 9 cut(s) 258, 346, 379, 393, 412, 465, 635, 698, 921
HgaI GACGC 8 cut(s) 291, 355, 406, 478, 593, 630, 659, 797
Hin1I GRCGYC 3 cut(s) 366, 489, 604
Hin1II CATG 1 cut(s) 18
HincII GTYRAC 2 cut(s) 61, 202
HindII GTYRAC 2 cut(s) 61, 202
HinfI GANTC 3 cut(s) 185, 517, 896
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 9 cut(s) 258, 346, 379, 393, 412, 465, 635, 698, 921
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 3 cut(s) 61, 202, 224
Hpy188I TCNGA 1 cut(s) 272
Hpy8I GTNNAC 3 cut(s) 61, 202, 224
Hpy99I CGWCG 9 cut(s) 238, 285, 341, 494, 497, 646, 714, 771, 791
HpyAV CCTTC 2 cut(s) 364, 773
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 4 cut(s) 207, 336, 559, 655
HpyCH4V TGCA 2 cut(s) 14, 146
HpyF3I CTNAG 3 cut(s) 25, 74, 122
HpySE526I ACGT 4 cut(s) 207, 336, 559, 655
Hsp92I GRCGYC 3 cut(s) 366, 489, 604
Hsp92II CATG 1 cut(s) 18
KroI GCCGGC 1 cut(s) 345
KroNI GCCGGC 1 cut(s) 347
KspAI GTTAAC 1 cut(s) 61
KspI CCGCGG 1 cut(s) 112
Kzo9I GATC 2 cut(s) 272, 288
LmnI GCTCC 8 cut(s) 35, 243, 382, 394, 458, 638, 796, 803
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 1 cut(s) 555
MaeI CTAG 3 cut(s) 87, 174, 903
MaeII ACGT 4 cut(s) 207, 336, 559, 655
MaeIII GTNAC 3 cut(s) 605, 842, 905
MalI GATC 2 cut(s) 274, 290
MauBI CGCGCGCG 1 cut(s) 478
MbiI CCGCTC 3 cut(s) 389, 779, 798
MboI GATC 2 cut(s) 272, 288
MboII GAAGA 3 cut(s) 149, 490, 566
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 3 cut(s) 363, 387, 592
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 4 cut(s) 52, 147, 168, 853
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 2 cut(s) 194, 511
Mox20I TGGCCA 1 cut(s) 137
MroNI GCCGGC 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 328
MscI TGGCCA 1 cut(s) 137
MseI TTAA 3 cut(s) 51, 60, 857
Msp20I TGGCCA 1 cut(s) 137
MspA1I CMGCKG 2 cut(s) 111, 850
MspI CCGG 9 cut(s) 258, 346, 379, 393, 412, 465, 635, 698, 921
MunI CAATTG 1 cut(s) 147
MvaI CCWGG 2 cut(s) 600, 830
NaeI GCCGGC 1 cut(s) 347
NciI CCSGG 9 cut(s) 379, 380, 393, 394, 465, 635, 636, 699, 922
NdeII GATC 2 cut(s) 272, 288
NgoMIV GCCGGC 1 cut(s) 345
NlaIII CATG 1 cut(s) 18
NlaIV GGNNCC 5 cut(s) 344, 384, 434, 582, 702
NmeAIII GCCGAG 6 cut(s) 342, 501, 516, 609, 685, 835
NmuCI GTSAC 1 cut(s) 605
NsbI TGCGCA 1 cut(s) 306
PauI GCGCGC 3 cut(s) 449, 476, 478
PcsI WCGNNNNNNNCGW 4 cut(s) 242, 363, 718, 772
PdiI GCCGGC 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 1 cut(s) 896
PflFI GACNNNGTC 1 cut(s) 206
PkrI GCNGC 6 cut(s) 113, 279, 309, 461, 488, 781
PleI GAGTC 2 cut(s) 193, 511
PpsI GAGTC 2 cut(s) 193, 511
PpuMI RGGWCCY 1 cut(s) 701
Psp5II RGGWCCY 1 cut(s) 701
Psp6I CCWGG 2 cut(s) 598, 828
PspEI GGTNACC 1 cut(s) 842
PspFI CCCAGC 1 cut(s) 931
PspGI CCWGG 2 cut(s) 598, 828
PspN4I GGNNCC 5 cut(s) 344, 384, 434, 582, 702
PspPI GGNCC 5 cut(s) 255, 343, 351, 701, 781
PspPPI RGGWCCY 1 cut(s) 701
PsyI GACNNNGTC 1 cut(s) 206
PteI GCGCGC 3 cut(s) 449, 476, 478
RsaI GTAC 2 cut(s) 223, 400
RsaNI GTAC 2 cut(s) 222, 399
SacII CCGCGG 1 cut(s) 112
SaqAI TTAA 3 cut(s) 51, 60, 857
SatI GCNGC 6 cut(s) 112, 278, 308, 460, 487, 780
Sau3AI GATC 2 cut(s) 272, 288
Sau96I GGNCC 5 cut(s) 255, 343, 351, 701, 781
SchI GAGTC 2 cut(s) 194, 511
SduI GDGCHC 3 cut(s) 363, 387, 592
SfaNI GCATC 1 cut(s) 555
Sfr303I CCGCGG 1 cut(s) 112
SgrAI CRCCGGYG 1 cut(s) 411
SgrBI CCGCGG 1 cut(s) 112
SinI GGWCC 2 cut(s) 255, 701
SmaI CCCGGG 3 cut(s) 380, 394, 636
Sse9I AATT 4 cut(s) 52, 147, 168, 853
SspMI CTAG 3 cut(s) 87, 174, 903
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 4 cut(s) 210, 339, 562, 658
TaqI TCGA 3 cut(s) 297, 549, 738
TasI AATT 4 cut(s) 52, 147, 168, 853
TatI WGTACW 1 cut(s) 221
TauI GCSGC 5 cut(s) 114, 280, 462, 489, 782
TfiI GAWTC 1 cut(s) 896
Tru1I TTAA 3 cut(s) 51, 60, 857
Tru9I TTAA 3 cut(s) 51, 60, 857
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 1 cut(s) 307
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 1 cut(s) 567
TspGWI ACGGA 2 cut(s) 46, 543
TspMI CCCGGG 3 cut(s) 378, 392, 634
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 2 cut(s) 255, 701
XapI RAATTY 2 cut(s) 168, 853
XmaI CCCGGG 3 cut(s) 378, 392, 634
XmnI GAANNNNTTC 1 cut(s) 328
XspI CTAG 3 cut(s) 87, 174, 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.