pycom2303g00060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002303
OG0002208
Physical Location & Seq
Reverse (-)
9955 .. 12871
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2303g00060.2

Sequence Viewer

Length: 1788 bp
ATGAGTGGTAATTGGGTTGTATCCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCATCGAGATTCGTCTCGTGCGCAAGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTTGCCGGCGGGAGGACGACATAGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGTGTAAGTATGAACAAATTCAGACTGTGAAACTTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCCAGAACGACCCGAGAACCCGTTCCGACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGATCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCATCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACGATCGGTGGTTTCCCAACCTCGGTTGCATGTTGTGCGCCTTCGTCGCGCTCCGAGCGGCCCGCGACGCTCCCCGCTCTGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

596

Amino Acids

65.35

Weight (kDa)

11.25

Isoelectric Point (pI)

62.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1188, 1218
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 2 cut(s) 760, 841
AccBSI CCGCTC 3 cut(s) 1268, 1662, 1681
AccI GTMKAC 2 cut(s) 383, 444
AclI AACGTT 1 cut(s) 143
AclWI GGATC 2 cut(s) 736, 1159
AcoI YGGCCR 5 cut(s) 515, 896, 991, 1014, 1690
AcsI RAATTY 3 cut(s) 408, 1047, 1736
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 4 cut(s) 1185, 1215, 1245, 1485
AfaI GTAC 4 cut(s) 66, 356, 1102, 1279
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 2 cut(s) 447, 468
AgsI TTSAA 5 cut(s) 547, 1040, 1130, 1436, 1702
AhdI GACNNNNNGTC 1 cut(s) 1713
AjnI CCWGG 5 cut(s) 502, 723, 847, 1479, 1711
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 4 cut(s) 969, 997, 1073, 1697
AluI AGCT 4 cut(s) 969, 997, 1073, 1697
Alw21I GWGCWC 3 cut(s) 214, 468, 563
Alw26I GTCTC 3 cut(s) 100, 706, 942
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 2 cut(s) 736, 1159
Ama87I CYCGRG 5 cut(s) 258, 1198, 1257, 1271, 1516
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 132, 372, 1175
ApoI RAATTY 3 cut(s) 408, 1047, 1736
ArsI GACNNNNNNTTYG 2 cut(s) 1567, 1599
AseI ATTAAT 1 cut(s) 651
AsiGI ACCGGT 2 cut(s) 447, 468
Asp700I GAANNNNTTC 2 cut(s) 408, 1207
AspS9I GGNCC 8 cut(s) 254, 429, 450, 1134, 1222, 1230, 1583, 1664
AsuC2I CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1581
AsuHPI GGTGA 1 cut(s) 1151
AvaI CYCGRG 5 cut(s) 258, 1198, 1257, 1271, 1516
AvaII GGWCC 5 cut(s) 254, 429, 450, 1134, 1583
BaeGI GKGCMC 3 cut(s) 468, 844, 1473
BalI TGGCCA 1 cut(s) 1016
BanI GGYRCC 2 cut(s) 760, 841
BanII GRGCYC 3 cut(s) 676, 1242, 1266
BauI CACGAG 2 cut(s) 97, 213
BbsI GAAGAC 3 cut(s) 301, 685, 1028
Bbv12I GWGCWC 3 cut(s) 214, 468, 563
BbvCI CCTCAGC 1 cut(s) 953
BbvI GCAGC 3 cut(s) 119, 359, 1162
BccI CCATC 4 cut(s) 102, 1025, 1431, 1533
BceAI ACGGC 4 cut(s) 43, 364, 883, 1479
BciT130I CCWGG 5 cut(s) 504, 725, 849, 1481, 1713
BciVI GTATCC 1 cut(s) 31
BcnI CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1581
BcoDI GTCTC 3 cut(s) 100, 706, 942
BfaI CTAG 4 cut(s) 698, 966, 1053, 1786
BfoI RGCGCY 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 31
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 7 cut(s) 133, 230, 373, 991, 1157, 1176, 1663
BlsI GCNGC 7 cut(s) 134, 231, 374, 992, 1158, 1177, 1664
Bme18I GGWCC 5 cut(s) 254, 429, 450, 1134, 1583
BmeRI GACNNNNNGTC 1 cut(s) 1713
BmeT110I CYCGRG 5 cut(s) 258, 1198, 1257, 1271, 1516
BmgT120I GGNCC 8 cut(s) 254, 429, 450, 1134, 1222, 1230, 1583, 1664
BmiI GGNNCC 9 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313, 1584
BmsI GCATC 5 cut(s) 56, 92, 272, 706, 1373
BpiI GAAGAC 3 cut(s) 301, 685, 1028
BplI GAGNNNNNCTC 2 cut(s) 218, 250
Bpu10I CCTNAGC 1 cut(s) 953
BpuMI CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1581
Bsa29I ATCGAT 1 cut(s) 1367
BsaHI GRCGYC 4 cut(s) 1185, 1215, 1245, 1485
BsaI GGTCTC 1 cut(s) 942
BsaWI WCCGGW 3 cut(s) 447, 468, 1136
Bse118I RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
Bse3DI GCAATG 1 cut(s) 1020
BseBI CCWGG 5 cut(s) 504, 725, 849, 1481, 1713
BseCI ATCGAT 1 cut(s) 1367
BseGI GGATG 5 cut(s) 287, 646, 742, 981, 1555
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 1 cut(s) 967
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 3 cut(s) 468, 844, 1473
BseX3I CGGCCG 1 cut(s) 1690
BseXI GCAGC 3 cut(s) 119, 359, 1162
BsgI GTGCAG 1 cut(s) 265
Bsh1285I CGRYCG 8 cut(s) 325, 333, 361, 387, 448, 473, 1610, 1693
BshNI GGYRCC 2 cut(s) 760, 841
BshTI ACCGGT 2 cut(s) 447, 468
BshVI ATCGAT 1 cut(s) 1367
BsiEI CGRYCG 8 cut(s) 325, 333, 361, 387, 448, 473, 1610, 1693
BsiHKAI GWGCWC 3 cut(s) 214, 468, 563
BsiHKCI CYCGRG 5 cut(s) 258, 1198, 1257, 1271, 1516
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 6 cut(s) 143, 203, 465, 1021, 1073, 1232
BsmAI GTCTC 3 cut(s) 100, 706, 942
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 6 cut(s) 143, 203, 465, 1021, 1073, 1232
Bso31I GGTCTC 1 cut(s) 942
BsoBI CYCGRG 5 cut(s) 258, 1198, 1257, 1271, 1516
Bsp1286I GDGCHC 8 cut(s) 214, 468, 563, 676, 844, 1242, 1266, 1473
Bsp1407I TGTACA 1 cut(s) 1100
Bsp143I GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
BspCNI CTCAG 1 cut(s) 966
BspDI ATCGAT 1 cut(s) 1367
BspLI GGNNCC 9 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313, 1584
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 2 cut(s) 736, 1159
BspT107I GGYRCC 2 cut(s) 760, 841
BspTNI GGTCTC 1 cut(s) 942
BsrBI CCGCTC 3 cut(s) 1268, 1662, 1681
BsrDI GCAATG 1 cut(s) 1020
BsrFI RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
BsrGI TGTACA 1 cut(s) 1100
BssAI RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
BssMI GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
BssNI GRCGYC 4 cut(s) 1185, 1215, 1245, 1485
BssSI CACGAG 2 cut(s) 97, 213
BssT1I CCWWGG 3 cut(s) 432, 636, 1307
Bst2BI CACGAG 2 cut(s) 97, 213
Bst2UI CCWGG 5 cut(s) 504, 725, 849, 1481, 1713
Bst4CI ACNGT 5 cut(s) 56, 362, 388, 418, 694
BstACI GRCGYC 4 cut(s) 1185, 1215, 1245, 1485
BstAPI GCANNNNNTGC 2 cut(s) 238, 1639
BstAUI TGTACA 1 cut(s) 1100
BstDEI CTNAG 3 cut(s) 904, 953, 1001
BstDSI CCRYGG 1 cut(s) 988
BstEII GGTNACC 1 cut(s) 1725
BstF5I GGATG 5 cut(s) 287, 646, 742, 981, 1555
BstH2I RGCGCY 1 cut(s) 537
BstKTI GATC 7 cut(s) 325, 333, 686, 731, 1154, 1328, 1610
BstMAI GTCTC 3 cut(s) 100, 706, 942
BstMBI GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
BstMCI CGRYCG 8 cut(s) 325, 333, 361, 387, 448, 473, 1610, 1693
BstNI CCWGG 5 cut(s) 504, 725, 849, 1481, 1713
BstNSI RCATGY 1 cut(s) 1637
BstPI GGTNACC 1 cut(s) 1725
BstSLI GKGCMC 3 cut(s) 468, 844, 1473
BstV1I GCAGC 3 cut(s) 119, 359, 1162
BstV2I GAAGAC 3 cut(s) 301, 685, 1028
BstZI CGGCCG 1 cut(s) 1690
Bsu15I ATCGAT 1 cut(s) 1367
BsuI GTATCC 1 cut(s) 31
BsuTUI ATCGAT 1 cut(s) 1367
BtgI CCRYGG 1 cut(s) 988
BtsCI GGATG 5 cut(s) 287, 646, 742, 981, 1555
BveI ACCTGC 1 cut(s) 137
Cfr10I RCCGGY 8 cut(s) 175, 184, 447, 468, 489, 757, 1224, 1290
Cfr13I GGNCC 8 cut(s) 254, 429, 450, 1134, 1222, 1230, 1583, 1664
Cfr42I CCGCGG 1 cut(s) 991
Cfr9I CCCGGG 2 cut(s) 1257, 1271
ClaI ATCGAT 1 cut(s) 1367
CpoI CGGWCCG 1 cut(s) 450
CseI GACGC 9 cut(s) 89, 359, 1170, 1234, 1285, 1474, 1512, 1541, 1680
Csp6I GTAC 4 cut(s) 65, 355, 1101, 1278
CspAI ACCGGT 2 cut(s) 447, 468
CspI CGGWCCG 1 cut(s) 450
CviAII CATG 3 cut(s) 594, 894, 1634
CviQI GTAC 4 cut(s) 65, 355, 1101, 1278
DdeI CTNAG 3 cut(s) 904, 953, 1001
DpnI GATC 7 cut(s) 324, 332, 685, 730, 1153, 1327, 1609
DpnII GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
DriI GACNNNNNGTC 1 cut(s) 1713
EaeI YGGCCR 5 cut(s) 515, 896, 991, 1014, 1690
EagI CGGCCG 1 cut(s) 1690
Eam1105I GACNNNNNGTC 1 cut(s) 1713
EciI GGCGGA 4 cut(s) 442, 744, 749, 1548
EclXI CGGCCG 1 cut(s) 1690
Eco130I CCWWGG 3 cut(s) 432, 636, 1307
Eco24I GRGCYC 3 cut(s) 676, 1242, 1266
Eco31I GGTCTC 1 cut(s) 942
Eco32I GATATC 1 cut(s) 1351
Eco47I GGWCC 5 cut(s) 254, 429, 450, 1134, 1583
Eco52I CGGCCG 1 cut(s) 1690
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 5 cut(s) 258, 1198, 1257, 1271, 1516
Eco91I GGTNACC 1 cut(s) 1725
EcoO109I RGGNCCY 1 cut(s) 1583
EcoO65I GGTNACC 1 cut(s) 1725
EcoRI GAATTC 1 cut(s) 1047
EcoRII CCWGG 5 cut(s) 502, 723, 847, 1479, 1711
EcoRV GATATC 1 cut(s) 1351
EcoT14I CCWWGG 3 cut(s) 432, 636, 1307
EcoT38I GRGCYC 3 cut(s) 676, 1242, 1266
ErhI CCWWGG 3 cut(s) 432, 636, 1307
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 3 cut(s) 597, 897, 1637
FaqI GGGAC 6 cut(s) 143, 203, 465, 1021, 1073, 1232
FatI CATG 3 cut(s) 593, 893, 1633
FblI GTMKAC 2 cut(s) 383, 444
Fnu4HI GCNGC 7 cut(s) 133, 230, 373, 991, 1157, 1176, 1663
FokI GGATG 5 cut(s) 294, 653, 749, 968, 1542
FriOI GRGCYC 3 cut(s) 676, 1242, 1266
Fsp4HI GCNGC 7 cut(s) 133, 230, 373, 991, 1157, 1176, 1663
FspBI CTAG 4 cut(s) 698, 966, 1053, 1786
FspI TGCGCA 1 cut(s) 103
GluI GCNGC 7 cut(s) 133, 230, 373, 991, 1157, 1176, 1663
HaeII RGCGCY 1 cut(s) 537
HgaI GACGC 9 cut(s) 89, 359, 1170, 1234, 1285, 1474, 1512, 1541, 1680
Hin1I GRCGYC 4 cut(s) 1185, 1215, 1245, 1485
Hin1II CATG 3 cut(s) 597, 897, 1637
HincII GTYRAC 4 cut(s) 384, 445, 940, 1081
HindII GTYRAC 4 cut(s) 384, 445, 940, 1081
HinfI GANTC 8 cut(s) 90, 751, 875, 1064, 1335, 1411, 1428, 1779
HpaI GTTAAC 1 cut(s) 940
HphI GGTGA 1 cut(s) 1151
Hpy166II GTNNAC 7 cut(s) 384, 445, 466, 940, 1081, 1103, 1420
Hpy188III TCNNGA 6 cut(s) 87, 313, 656, 872, 1189, 1518
Hpy8I GTNNAC 7 cut(s) 384, 445, 466, 940, 1081, 1103, 1420
HpyAV CCTTC 6 cut(s) 131, 289, 493, 831, 1243, 1655
HpyCH4III ACNGT 5 cut(s) 56, 362, 388, 418, 694
HpyCH4IV ACGT 5 cut(s) 143, 1086, 1185, 1215, 1377
HpyCH4V TGCA 6 cut(s) 246, 466, 893, 1025, 1408, 1633
HpyF3I CTNAG 3 cut(s) 904, 953, 1001
HpySE526I ACGT 5 cut(s) 143, 1086, 1185, 1215, 1377
Hsp92I GRCGYC 4 cut(s) 1185, 1215, 1245, 1485
Hsp92II CATG 3 cut(s) 597, 897, 1637
KroI GCCGGC 5 cut(s) 175, 184, 489, 757, 1224
KroNI GCCGGC 5 cut(s) 177, 186, 491, 759, 1226
KspAI GTTAAC 1 cut(s) 940
KspI CCGCGG 1 cut(s) 991
Kzo9I GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
Lsp1109I GCAGC 3 cut(s) 119, 359, 1162
LweI GCATC 5 cut(s) 56, 92, 272, 706, 1373
MaeI CTAG 4 cut(s) 698, 966, 1053, 1786
MaeII ACGT 5 cut(s) 143, 1086, 1185, 1215, 1377
MaeIII GTNAC 4 cut(s) 538, 739, 1486, 1725
MalI GATC 7 cut(s) 324, 332, 685, 730, 1153, 1327, 1609
MbiI CCGCTC 3 cut(s) 1268, 1662, 1681
MboI GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
MboII GAAGA 5 cut(s) 301, 559, 690, 1028, 1384
MfeI CAATTG 1 cut(s) 1026
MhlI GDGCHC 8 cut(s) 214, 468, 563, 676, 844, 1242, 1266, 1473
MlsI TGGCCA 1 cut(s) 1016
MluI ACGCGT 1 cut(s) 351
MluNI TGGCCA 1 cut(s) 1016
MlyI GAGTC 4 cut(s) 745, 1073, 1329, 1437
MmeI TCCRAC 2 cut(s) 48, 1236
Mox20I TGGCCA 1 cut(s) 1016
MroNI GCCGGC 5 cut(s) 175, 184, 489, 757, 1224
MroXI GAANNNNTTC 2 cut(s) 408, 1207
MscI TGGCCA 1 cut(s) 1016
MseI TTAA 6 cut(s) 510, 651, 822, 930, 939, 1740
MslI CAYNNNNRTG 1 cut(s) 592
Msp20I TGGCCA 1 cut(s) 1016
MspA1I CMGCKG 3 cut(s) 372, 990, 1733
MunI CAATTG 1 cut(s) 1026
MvaI CCWGG 5 cut(s) 504, 725, 849, 1481, 1713
NaeI GCCGGC 5 cut(s) 177, 186, 491, 759, 1226
NciI CCSGG 8 cut(s) 221, 519, 793, 1258, 1259, 1272, 1273, 1581
NdeII GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
NgoMIV GCCGGC 5 cut(s) 175, 184, 489, 757, 1224
NlaIII CATG 3 cut(s) 597, 897, 1637
NlaIV GGNNCC 9 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313, 1584
NmeAIII GCCGAG 6 cut(s) 1221, 1319, 1334, 1490, 1567, 1718
NmuCI GTSAC 1 cut(s) 1486
NsbI TGCGCA 1 cut(s) 103
NspI RCATGY 1 cut(s) 1637
PcsI WCGNNNNNNNCGW 7 cut(s) 350, 354, 1121, 1179, 1191, 1242, 1600
PdiI GCCGGC 5 cut(s) 177, 186, 491, 759, 1226
PdmI GAANNNNTTC 2 cut(s) 408, 1207
PfeI GAWTC 4 cut(s) 90, 875, 1411, 1779
Pfl23II CGTACG 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 1085
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 447, 468
PkrI GCNGC 7 cut(s) 134, 231, 374, 992, 1158, 1177, 1664
Ple19I CGATCG 3 cut(s) 325, 333, 1610
PleI GAGTC 4 cut(s) 745, 1072, 1329, 1436
PpsI GAGTC 4 cut(s) 745, 1072, 1329, 1436
PpuMI RGGWCCY 1 cut(s) 1583
PshBI ATTAAT 1 cut(s) 651
Psp1406I AACGTT 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 1583
Psp6I CCWGG 5 cut(s) 502, 723, 847, 1479, 1711
PspEI GGTNACC 1 cut(s) 1725
PspGI CCWGG 5 cut(s) 502, 723, 847, 1479, 1711
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 9 cut(s) 35, 762, 790, 843, 858, 1223, 1263, 1313, 1584
PspPI GGNCC 8 cut(s) 254, 429, 450, 1134, 1222, 1230, 1583, 1664
PspPPI RGGWCCY 1 cut(s) 1583
PsyI GACNNNGTC 1 cut(s) 1085
PvuI CGATCG 3 cut(s) 325, 333, 1610
RsaI GTAC 4 cut(s) 66, 356, 1102, 1279
RsaNI GTAC 4 cut(s) 65, 355, 1101, 1278
RseI CAYNNNNRTG 1 cut(s) 592
Rsr2I CGGWCCG 1 cut(s) 450
RsrII CGGWCCG 1 cut(s) 450
SacII CCGCGG 1 cut(s) 991
SalI GTCGAC 2 cut(s) 382, 443
SaqAI TTAA 6 cut(s) 510, 651, 822, 930, 939, 1740
SatI GCNGC 7 cut(s) 133, 230, 373, 991, 1157, 1176, 1663
Sau3AI GATC 7 cut(s) 322, 330, 683, 728, 1151, 1325, 1607
Sau96I GGNCC 8 cut(s) 254, 429, 450, 1134, 1222, 1230, 1583, 1664
SchI GAGTC 4 cut(s) 745, 1073, 1329, 1437
SduI GDGCHC 8 cut(s) 214, 468, 563, 676, 844, 1242, 1266, 1473
SfaNI GCATC 5 cut(s) 56, 92, 272, 706, 1373
Sfr303I CCGCGG 1 cut(s) 991
SgrAI CRCCGGYG 1 cut(s) 1290
SgrBI CCGCGG 1 cut(s) 991
SinI GGWCC 5 cut(s) 254, 429, 450, 1134, 1583
SmaI CCCGGG 2 cut(s) 1259, 1273
SmiMI CAYNNNNRTG 1 cut(s) 592
SspMI CTAG 4 cut(s) 698, 966, 1053, 1786
StyI CCWWGG 3 cut(s) 432, 636, 1307
TaaI ACNGT 5 cut(s) 56, 362, 388, 418, 694
TaiI ACGT 5 cut(s) 146, 1089, 1188, 1218, 1380
TaqII GACCGA 1 cut(s) 242
TatI WGTACW 1 cut(s) 1100
TauI GCSGC 4 cut(s) 232, 993, 1159, 1665
TfiI GAWTC 4 cut(s) 90, 875, 1411, 1779
Tru1I TTAA 6 cut(s) 510, 651, 822, 930, 939, 1740
Tru9I TTAA 6 cut(s) 510, 651, 822, 930, 939, 1740
TseFI GTSAC 1 cut(s) 1486
TseI GCWGC 3 cut(s) 132, 372, 1175
Tsp45I GTSAC 1 cut(s) 1486
TspDTI ATGAA 4 cut(s) 64, 417, 637, 1385
TspGWI ACGGA 5 cut(s) 26, 256, 849, 925, 1361
TspMI CCCGGG 2 cut(s) 1257, 1271
Tth111I GACNNNGTC 1 cut(s) 1085
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 5 cut(s) 254, 429, 450, 1134, 1583
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 3 cut(s) 408, 1047, 1736
XceI RCATGY 1 cut(s) 1637
XmaI CCCGGG 2 cut(s) 1257, 1271
XmiI GTMKAC 2 cut(s) 383, 444
XmnI GAANNNNTTC 2 cut(s) 408, 1207
XspI CTAG 4 cut(s) 698, 966, 1053, 1786
ZraI GACGTC 2 cut(s) 1186, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.