pycom12597g00040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012597
OG0002208
Physical Location & Seq
Forward (+)
9621 .. 10040
420 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12597g00040.1

Sequence Viewer

Length: 420 bp
ATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCATCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACGATCGGTGGTTTCCCAACCTCGGTTGCATGTTGTGCGCCTTCGTCGCGCTCCGAGCGGCCCGCGACGCTCCCCGCTCTGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.62

Weight (kDa)

12.0

Isoelectric Point (pI)

97.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 294, 313
AccII CGCG 6 cut(s) 140, 194, 196, 285, 301, 338
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 149, 258, 349
AgsI TTSAA 2 cut(s) 68, 334
AhdI GACNNNNNGTC 1 cut(s) 345
AjnI CCWGG 2 cut(s) 111, 343
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 4 cut(s) 94, 173, 295, 322
ApoI RAATTY 1 cut(s) 368
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspLEI GCGC 5 cut(s) 81, 142, 196, 276, 287
AspS9I GGNCC 2 cut(s) 215, 296
AsuC2I CCSGG 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 105
BccI CCATC 2 cut(s) 63, 165
BceAI ACGGC 1 cut(s) 111
BciT130I CCWGG 2 cut(s) 113, 345
BcnI CCSGG 1 cut(s) 213
BfaI CTAG 1 cut(s) 418
BisI GCNGC 1 cut(s) 295
BlsI GCNGC 1 cut(s) 296
Bme1390I CCNGG 3 cut(s) 113, 213, 345
Bme18I GGWCC 1 cut(s) 215
BmeRI GACNNNNNGTC 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 215, 296
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 3 cut(s) 113, 213, 345
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 6 cut(s) 97, 112, 147, 218, 257, 343
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 258, 349
BseBI CCWGG 2 cut(s) 113, 345
BseDI CCNNGG 6 cut(s) 97, 112, 147, 218, 257, 343
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 3 cut(s) 149, 258, 349
BseSI GKGCMC 1 cut(s) 105
BseX3I CGGCCG 1 cut(s) 322
Bsh1236I CGCG 6 cut(s) 140, 194, 196, 285, 301, 338
Bsh1285I CGRYCG 2 cut(s) 242, 325
BshFI GGCC 4 cut(s) 96, 175, 297, 324
BsiEI CGRYCG 2 cut(s) 242, 325
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 149, 258, 349
BsnI GGCC 4 cut(s) 96, 175, 297, 324
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 1 cut(s) 239
BspANI GGCC 4 cut(s) 96, 175, 297, 324
BspFNI CGCG 6 cut(s) 140, 194, 196, 285, 301, 338
BspLI GGNNCC 1 cut(s) 216
BsrBI CCGCTC 2 cut(s) 294, 313
BssECI CCNNGG 6 cut(s) 97, 112, 147, 218, 257, 343
BssMI GATC 1 cut(s) 239
BssNI GRCGYC 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 113, 345
BstACI GRCGYC 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 105, 109, 299
BstEII GGTNACC 1 cut(s) 357
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 6 cut(s) 140, 194, 196, 285, 301, 338
BstHHI GCGC 5 cut(s) 81, 142, 196, 276, 287
BstKTI GATC 1 cut(s) 242
BstMBI GATC 1 cut(s) 239
BstMCI CGRYCG 2 cut(s) 242, 325
BstNI CCWGG 2 cut(s) 113, 345
BstNSI RCATGY 1 cut(s) 269
BstPI GGTNACC 1 cut(s) 357
BstSCI CCNGG 3 cut(s) 111, 211, 343
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 6 cut(s) 140, 194, 196, 285, 301, 338
BstZI CGGCCG 1 cut(s) 322
BsuRI GGCC 4 cut(s) 96, 175, 297, 324
BtsCI GGATG 1 cut(s) 187
Cac8I GCNNGC 3 cut(s) 105, 109, 299
CfoI GCGC 5 cut(s) 81, 142, 196, 276, 287
Cfr13I GGNCC 2 cut(s) 215, 296
CseI GACGC 4 cut(s) 106, 144, 173, 312
CviAII CATG 1 cut(s) 266
CviJI RGCY 6 cut(s) 88, 96, 175, 297, 324, 329
CviKI_1 RGCY 6 cut(s) 88, 96, 175, 297, 324, 329
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
DriI GACNNNNNGTC 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 322
EagI CGGCCG 1 cut(s) 322
Eam1105I GACNNNNNGTC 1 cut(s) 345
EciI GGCGGA 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 215
Eco52I CGGCCG 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 148
Eco91I GGTNACC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 111, 343
FaeI CATG 1 cut(s) 269
FaiI YATR 2 cut(s) 267, 378
FatI CATG 1 cut(s) 265
FauI CCCGC 6 cut(s) 145, 199, 306, 318, 346, 370
Fnu4HI GCNGC 1 cut(s) 295
FokI GGATG 1 cut(s) 174
Fsp4HI GCNGC 1 cut(s) 295
FspBI CTAG 1 cut(s) 418
GlaI GCGC 5 cut(s) 80, 141, 195, 275, 286
GluI GCNGC 1 cut(s) 295
HaeIII GGCC 4 cut(s) 96, 175, 297, 324
HapII CCGG 1 cut(s) 212
HgaI GACGC 4 cut(s) 106, 144, 173, 312
HhaI GCGC 5 cut(s) 81, 142, 196, 276, 287
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 5 cut(s) 79, 140, 194, 274, 285
HinP1I GCGC 5 cut(s) 79, 140, 194, 274, 285
HinfI GANTC 3 cut(s) 43, 60, 411
HpaII CCGG 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 5 cut(s) 160, 228, 240, 285, 306
HpyAV CCTTC 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 40, 265
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 5 cut(s) 79, 140, 194, 274, 285
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 3 cut(s) 152, 292, 311
LpnPI CCDG 7 cut(s) 98, 121, 125, 225, 330, 335, 357
MaeI CTAG 1 cut(s) 418
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 2 cut(s) 118, 357
MalI GATC 1 cut(s) 241
MbiI CCGCTC 2 cut(s) 294, 313
MboI GATC 1 cut(s) 239
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 35, 368
MlyI GAGTC 1 cut(s) 69
MnlI CCTC 8 cut(s) 92, 153, 157, 186, 228, 267, 384, 401
MseI TTAA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 3 cut(s) 113, 213, 345
MvaI CCWGG 2 cut(s) 113, 345
MvnI CGCG 6 cut(s) 140, 194, 196, 285, 301, 338
NciI CCSGG 1 cut(s) 213
NdeII GATC 1 cut(s) 239
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 1 cut(s) 216
NmeAIII GCCGAG 3 cut(s) 122, 199, 350
NmuCI GTSAC 1 cut(s) 118
NspI RCATGY 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PfeI GAWTC 2 cut(s) 43, 411
PkrI GCNGC 1 cut(s) 296
Ple19I CGATCG 1 cut(s) 242
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 111, 343
PspEI GGTNACC 1 cut(s) 357
PspGI CCWGG 2 cut(s) 111, 343
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 215, 296
PspPPI RGGWCCY 1 cut(s) 215
PvuI CGATCG 1 cut(s) 242
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 295
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 2 cut(s) 215, 296
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 3 cut(s) 113, 213, 345
SduI GDGCHC 1 cut(s) 105
SetI ASST 4 cut(s) 12, 259, 331, 349
SinI GGWCC 1 cut(s) 215
Sse9I AATT 2 cut(s) 35, 368
SspMI CTAG 1 cut(s) 418
StyD4I CCNGG 3 cut(s) 111, 211, 343
TaiI ACGT 1 cut(s) 12
TaqI TCGA 1 cut(s) 58
TasI AATT 2 cut(s) 35, 368
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 2 cut(s) 43, 411
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 269
XspI CTAG 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.