pycom2246g00200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002246
OG0002208
Physical Location & Seq
Forward (+)
54138 .. 55762
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2246g00200.5

Sequence Viewer

Length: 1197 bp
ATGATTAACAGGGACAGTCGGGGTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCATCACCAGGAGTGGAGCCTGCGTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCACATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGTCGTCCTCTTATCCCGGGAGCCCGCTCCCGGGCGTACAAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCGACCCGCGCGGTTGGCACAAATGCCGGGTGCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCCAACCTCGGTTGCCCGTTGTGCGCTTTCGTCGCGCTCCGAGCGGCCCGCGACGCTCCTCGCTCGGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

399

Amino Acids

42.76

Weight (kDa)

11.7

Isoelectric Point (pI)

71.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 133
AccBSI CCGCTC 2 cut(s) 616, 1071
AccI GTMKAC 1 cut(s) 964
AclWI GGATC 2 cut(s) 109, 496
AcoI YGGCCR 3 cut(s) 257, 352, 1099
AcsI RAATTY 2 cut(s) 384, 1145
AcyI GRCGYC 2 cut(s) 716, 894
AfaI GTAC 2 cut(s) 439, 627
AfiI CCNNNNNNNGG 8 cut(s) 103, 566, 567, 619, 620, 697, 926, 1126
AgsI TTSAA 4 cut(s) 377, 467, 845, 1111
AhdI GACNNNNNGTC 1 cut(s) 1122
AjiI CACGTC 1 cut(s) 564
AjnI CCWGG 4 cut(s) 96, 220, 888, 1120
AluBI AGCT 3 cut(s) 330, 410, 1106
AluI AGCT 3 cut(s) 330, 410, 1106
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 2 cut(s) 79, 303
AlwI GGATC 2 cut(s) 109, 496
Ama87I CYCGRG 4 cut(s) 546, 605, 619, 925
AoxI GGCC 8 cut(s) 257, 352, 570, 578, 871, 950, 1072, 1099
ApeKI GCWGC 2 cut(s) 512, 534
ApoI RAATTY 2 cut(s) 384, 1145
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 4 cut(s) 471, 570, 578, 1073
AsuC2I CCSGG 7 cut(s) 166, 606, 607, 620, 621, 692, 990
AsuHPI GGTGA 2 cut(s) 210, 488
AvaI CYCGRG 4 cut(s) 546, 605, 619, 925
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 882
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 3 cut(s) 49, 590, 614
BbsI GAAGAC 3 cut(s) 58, 365, 717
Bbv12I GWGCWC 1 cut(s) 997
BbvCI CCTCAGC 1 cut(s) 314
BbvI GCAGC 2 cut(s) 499, 546
BccI CCATC 2 cut(s) 840, 942
BceAI ACGGC 2 cut(s) 244, 888
BciT130I CCWGG 4 cut(s) 98, 222, 890, 1122
BcnI CCSGG 7 cut(s) 166, 606, 607, 620, 621, 692, 990
BcoDI GTCTC 2 cut(s) 79, 303
BfaI CTAG 4 cut(s) 71, 327, 390, 1195
BfuAI ACCTGC 1 cut(s) 537
BisI GCNGC 7 cut(s) 352, 494, 513, 535, 687, 714, 1072
BlsI GCNGC 7 cut(s) 353, 495, 514, 536, 688, 715, 1073
Bme18I GGWCC 1 cut(s) 471
BmeRI GACNNNNNGTC 1 cut(s) 1122
BmeT110I CYCGRG 4 cut(s) 546, 605, 619, 925
BmgBI CACGTC 1 cut(s) 564
BmgT120I GGNCC 4 cut(s) 471, 570, 578, 1073
BmiI GGNNCC 6 cut(s) 135, 163, 231, 571, 611, 661
BmsI GCATC 3 cut(s) 79, 224, 782
BpiI GAAGAC 3 cut(s) 58, 365, 717
Bpu10I CCTNAGC 1 cut(s) 314
BpuMI CCSGG 7 cut(s) 166, 606, 607, 620, 621, 692, 990
Bsa29I ATCGAT 1 cut(s) 776
BsaHI GRCGYC 2 cut(s) 716, 894
BsaI GGTCTC 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 473
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 566, 567, 619, 620, 697, 926, 1126
Bse118I RCCGGY 3 cut(s) 130, 572, 638
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 4 cut(s) 98, 222, 890, 1122
BseCI ATCGAT 1 cut(s) 776
BseGI GGATG 2 cut(s) 115, 342
BseLI CCNNNNNNNGG 8 cut(s) 103, 566, 567, 619, 620, 697, 926, 1126
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 328
BsePI GCGCGC 3 cut(s) 676, 703, 705
BseRI GAGGAG 1 cut(s) 1075
BseSI GKGCMC 1 cut(s) 882
BseX3I CGGCCG 1 cut(s) 1099
BseXI GCAGC 2 cut(s) 499, 546
Bsh1285I CGRYCG 1 cut(s) 1102
BshFI GGCC 8 cut(s) 259, 354, 572, 580, 873, 952, 1074, 1101
BshNI GGYRCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 776
BsiEI CGRYCG 1 cut(s) 1102
BsiHKAI GWGCWC 1 cut(s) 997
BsiHKCI CYCGRG 4 cut(s) 546, 605, 619, 925
BsiSI CCGG 9 cut(s) 131, 165, 474, 573, 606, 620, 639, 692, 989
BslFI GGGAC 2 cut(s) 26, 410
BslI CCNNNNNNNGG 8 cut(s) 103, 566, 567, 619, 620, 697, 926, 1126
BsmAI GTCTC 2 cut(s) 79, 303
BsmFI GGGAC 2 cut(s) 26, 410
BsnI GGCC 8 cut(s) 259, 354, 572, 580, 873, 952, 1074, 1101
Bso31I GGTCTC 1 cut(s) 303
BsoBI CYCGRG 4 cut(s) 546, 605, 619, 925
Bsp1286I GDGCHC 5 cut(s) 49, 590, 614, 882, 997
Bsp1407I TGTACA 1 cut(s) 437
Bsp143I GATC 3 cut(s) 56, 101, 488
BspANI GGCC 8 cut(s) 259, 354, 572, 580, 873, 952, 1074, 1101
BspCNI CTCAG 1 cut(s) 327
BspDI ATCGAT 1 cut(s) 776
BspLI GGNNCC 6 cut(s) 135, 163, 231, 571, 611, 661
BspMI ACCTGC 1 cut(s) 537
BspPI GGATC 2 cut(s) 109, 496
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 303
BsrBI CCGCTC 2 cut(s) 616, 1071
BsrDI GCAATG 1 cut(s) 357
BsrFI RCCGGY 3 cut(s) 130, 572, 638
BsrGI TGTACA 1 cut(s) 437
BssAI RCCGGY 3 cut(s) 130, 572, 638
BssHII GCGCGC 3 cut(s) 676, 703, 705
BssMI GATC 3 cut(s) 56, 101, 488
BssNI GRCGYC 2 cut(s) 716, 894
BssT1I CCWWGG 1 cut(s) 655
Bst2UI CCWGG 4 cut(s) 98, 222, 890, 1122
Bst4CI ACNGT 3 cut(s) 17, 67, 700
BstACI GRCGYC 2 cut(s) 716, 894
BstAUI TGTACA 1 cut(s) 437
BstDEI CTNAG 2 cut(s) 265, 314
BstDSI CCRYGG 1 cut(s) 349
BstEII GGTNACC 1 cut(s) 1134
BstF5I GGATG 2 cut(s) 115, 342
BstKTI GATC 3 cut(s) 59, 104, 491
BstMAI GTCTC 2 cut(s) 79, 303
BstMBI GATC 3 cut(s) 56, 101, 488
BstMCI CGRYCG 1 cut(s) 1102
BstNI CCWGG 4 cut(s) 98, 222, 890, 1122
BstPI GGTNACC 1 cut(s) 1134
BstSLI GKGCMC 1 cut(s) 882
BstV1I GCAGC 2 cut(s) 499, 546
BstV2I GAAGAC 3 cut(s) 58, 365, 717
BstZI CGGCCG 1 cut(s) 1099
Bsu15I ATCGAT 1 cut(s) 776
BsuRI GGCC 8 cut(s) 259, 354, 572, 580, 873, 952, 1074, 1101
BsuTUI ATCGAT 1 cut(s) 776
BtgI CCRYGG 1 cut(s) 349
BtrI CACGTC 1 cut(s) 564
BtsCI GGATG 2 cut(s) 115, 342
BveI ACCTGC 1 cut(s) 537
Cfr10I RCCGGY 3 cut(s) 130, 572, 638
Cfr13I GGNCC 4 cut(s) 471, 570, 578, 1073
Cfr42I CCGCGG 1 cut(s) 352
Cfr9I CCCGGG 2 cut(s) 605, 619
ClaI ATCGAT 1 cut(s) 776
CseI GACGC 8 cut(s) 225, 507, 633, 705, 883, 921, 950, 1089
Csp6I GTAC 2 cut(s) 438, 626
CviAII CATG 1 cut(s) 255
CviQI GTAC 2 cut(s) 438, 626
DdeI CTNAG 2 cut(s) 265, 314
DpnI GATC 3 cut(s) 58, 103, 490
DpnII GATC 3 cut(s) 56, 101, 488
DriI GACNNNNNGTC 1 cut(s) 1122
EaeI YGGCCR 3 cut(s) 257, 352, 1099
EagI CGGCCG 1 cut(s) 1099
Eam1105I GACNNNNNGTC 1 cut(s) 1122
EciI GGCGGA 3 cut(s) 117, 122, 957
EclXI CGGCCG 1 cut(s) 1099
Eco130I CCWWGG 1 cut(s) 655
Eco24I GRGCYC 3 cut(s) 49, 590, 614
Eco31I GGTCTC 1 cut(s) 303
Eco32I GATATC 1 cut(s) 760
Eco47I GGWCC 1 cut(s) 471
Eco52I CGGCCG 1 cut(s) 1099
Eco88I CYCGRG 4 cut(s) 546, 605, 619, 925
Eco91I GGTNACC 1 cut(s) 1134
EcoO65I GGTNACC 1 cut(s) 1134
EcoRI GAATTC 1 cut(s) 384
EcoRII CCWGG 4 cut(s) 96, 220, 888, 1120
EcoRV GATATC 1 cut(s) 760
EcoT14I CCWWGG 1 cut(s) 655
EcoT38I GRGCYC 3 cut(s) 49, 590, 614
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 1 cut(s) 258
FaiI YATR 7 cut(s) 83, 141, 176, 242, 256, 333, 1155
FaqI GGGAC 2 cut(s) 26, 410
FatI CATG 1 cut(s) 254
FblI GTMKAC 1 cut(s) 964
Fnu4HI GCNGC 7 cut(s) 352, 494, 513, 535, 687, 714, 1072
FokI GGATG 2 cut(s) 122, 329
FriOI GRGCYC 3 cut(s) 49, 590, 614
Fsp4HI GCNGC 7 cut(s) 352, 494, 513, 535, 687, 714, 1072
FspBI CTAG 4 cut(s) 71, 327, 390, 1195
FspI TGCGCA 1 cut(s) 533
GluI GCNGC 7 cut(s) 352, 494, 513, 535, 687, 714, 1072
HaeIII GGCC 8 cut(s) 259, 354, 572, 580, 873, 952, 1074, 1101
HapII CCGG 9 cut(s) 131, 165, 474, 573, 606, 620, 639, 692, 989
HgaI GACGC 8 cut(s) 225, 507, 633, 705, 883, 921, 950, 1089
Hin1I GRCGYC 2 cut(s) 716, 894
Hin1II CATG 1 cut(s) 258
HincII GTYRAC 3 cut(s) 301, 418, 965
HindII GTYRAC 3 cut(s) 301, 418, 965
HinfI GANTC 6 cut(s) 124, 401, 744, 820, 837, 1188
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 9 cut(s) 131, 165, 474, 573, 606, 620, 639, 692, 989
HphI GGTGA 2 cut(s) 210, 488
Hpy166II GTNNAC 5 cut(s) 301, 418, 440, 829, 965
Hpy188I TCNGA 3 cut(s) 61, 488, 1068
Hpy188III TCNNGA 2 cut(s) 29, 927
Hpy8I GTNNAC 5 cut(s) 301, 418, 440, 829, 965
HpyAV CCTTC 2 cut(s) 204, 591
HpyCH4III ACNGT 3 cut(s) 17, 67, 700
HpyCH4IV ACGT 3 cut(s) 423, 563, 786
HpyCH4V TGCA 3 cut(s) 254, 362, 817
HpyF3I CTNAG 2 cut(s) 265, 314
HpySE526I ACGT 3 cut(s) 423, 563, 786
Hsp92I GRCGYC 2 cut(s) 716, 894
Hsp92II CATG 1 cut(s) 258
KroI GCCGGC 2 cut(s) 130, 572
KroNI GCCGGC 2 cut(s) 132, 574
KspAI GTTAAC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 352
Kzo9I GATC 3 cut(s) 56, 101, 488
LmnI GCTCC 9 cut(s) 229, 275, 459, 609, 621, 685, 929, 1069, 1088
Lsp1109I GCAGC 2 cut(s) 499, 546
LweI GCATC 3 cut(s) 79, 224, 782
MaeI CTAG 4 cut(s) 71, 327, 390, 1195
MaeII ACGT 3 cut(s) 423, 563, 786
MaeIII GTNAC 3 cut(s) 112, 895, 1134
MalI GATC 3 cut(s) 58, 103, 490
MauBI CGCGCGCG 1 cut(s) 705
MbiI CCGCTC 2 cut(s) 616, 1071
MboI GATC 3 cut(s) 56, 101, 488
MboII GAAGA 4 cut(s) 63, 365, 717, 793
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 5 cut(s) 49, 590, 614, 882, 997
MluCI AATT 6 cut(s) 201, 292, 363, 384, 812, 1145
MlyI GAGTC 4 cut(s) 118, 410, 738, 846
MmeI TCCRAC 1 cut(s) 1054
MroNI GCCGGC 2 cut(s) 130, 572
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 5 cut(s) 6, 195, 291, 300, 1149
MspA1I CMGCKG 2 cut(s) 351, 1142
MspI CCGG 9 cut(s) 131, 165, 474, 573, 606, 620, 639, 692, 989
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 4 cut(s) 98, 222, 890, 1122
NaeI GCCGGC 2 cut(s) 132, 574
NciI CCSGG 7 cut(s) 166, 606, 607, 620, 621, 692, 990
NdeII GATC 3 cut(s) 56, 101, 488
NgoMIV GCCGGC 2 cut(s) 130, 572
NlaIII CATG 1 cut(s) 258
NlaIV GGNNCC 6 cut(s) 135, 163, 231, 571, 611, 661
NmeAIII GCCGAG 6 cut(s) 728, 743, 899, 976, 1071, 1127
NmuCI GTSAC 1 cut(s) 895
NsbI TGCGCA 1 cut(s) 533
PauI GCGCGC 3 cut(s) 676, 703, 705
PcsI WCGNNNNNNNCGW 3 cut(s) 458, 590, 1009
PdiI GCCGGC 2 cut(s) 132, 574
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 820, 1188
PflFI GACNNNGTC 1 cut(s) 422
PkrI GCNGC 7 cut(s) 353, 495, 514, 536, 688, 715, 1073
PleI GAGTC 4 cut(s) 118, 409, 738, 845
PpsI GAGTC 4 cut(s) 118, 409, 738, 845
Psp6I CCWGG 4 cut(s) 96, 220, 888, 1120
PspEI GGTNACC 1 cut(s) 1134
PspGI CCWGG 4 cut(s) 96, 220, 888, 1120
PspN4I GGNNCC 6 cut(s) 135, 163, 231, 571, 611, 661
PspPI GGNCC 4 cut(s) 471, 570, 578, 1073
PsyI GACNNNGTC 1 cut(s) 422
PteI GCGCGC 3 cut(s) 676, 703, 705
RsaI GTAC 2 cut(s) 439, 627
RsaNI GTAC 2 cut(s) 438, 626
SacII CCGCGG 1 cut(s) 352
SalI GTCGAC 1 cut(s) 963
SaqAI TTAA 5 cut(s) 6, 195, 291, 300, 1149
SatI GCNGC 7 cut(s) 352, 494, 513, 535, 687, 714, 1072
Sau3AI GATC 3 cut(s) 56, 101, 488
Sau96I GGNCC 4 cut(s) 471, 570, 578, 1073
SchI GAGTC 4 cut(s) 118, 410, 738, 846
SduI GDGCHC 5 cut(s) 49, 590, 614, 882, 997
SfaNI GCATC 3 cut(s) 79, 224, 782
Sfr303I CCGCGG 1 cut(s) 352
SgrAI CRCCGGYG 1 cut(s) 638
SgrBI CCGCGG 1 cut(s) 352
SinI GGWCC 1 cut(s) 471
SmaI CCCGGG 2 cut(s) 607, 621
Sse9I AATT 6 cut(s) 201, 292, 363, 384, 812, 1145
SspMI CTAG 4 cut(s) 71, 327, 390, 1195
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 3 cut(s) 17, 67, 700
TaiI ACGT 3 cut(s) 426, 566, 789
TaqI TCGA 5 cut(s) 49, 524, 776, 835, 964
TasI AATT 6 cut(s) 201, 292, 363, 384, 812, 1145
TatI WGTACW 1 cut(s) 437
TauI GCSGC 5 cut(s) 354, 496, 689, 716, 1074
TfiI GAWTC 2 cut(s) 820, 1188
Tru1I TTAA 5 cut(s) 6, 195, 291, 300, 1149
Tru9I TTAA 5 cut(s) 6, 195, 291, 300, 1149
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 2 cut(s) 512, 534
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 1 cut(s) 794
TspGWI ACGGA 3 cut(s) 222, 286, 770
TspMI CCCGGG 2 cut(s) 605, 619
Tth111I GACNNNGTC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 2 cut(s) 384, 1145
XmaI CCCGGG 2 cut(s) 605, 619
XmiI GTMKAC 1 cut(s) 964
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 4 cut(s) 71, 327, 390, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.