pycom2158g00030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002158
OG0002208
Physical Location & Seq
Forward (+)
4984 .. 9144
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2158g00030.1

Sequence Viewer

Length: 1566 bp
ATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACGCCGACGTCGAAGAGGAATGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATTTATAGTTTGTTTGATGGTATCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACAGCCATCGTGCTGGCGACGCATCATTCAAATATCTCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGATTTCGGTCCTATTCTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTCGCGTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTCAACTAGCCCCGAAAATGGATGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

522

Amino Acids

55.8

Weight (kDa)

10.39

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 362, 1249
AatII GACGTC 1 cut(s) 366
Acc16I TGCGCA 2 cut(s) 103, 1262
Acc36I ACCTGC 2 cut(s) 137, 1266
AccB1I GGYRCC 2 cut(s) 994, 1075
AccBSI CCGCTC 1 cut(s) 1345
AccI GTMKAC 4 cut(s) 328, 545, 660, 844
AclI AACGTT 1 cut(s) 143
AclWI GGATC 4 cut(s) 383, 600, 824, 970
AcoI YGGCCR 3 cut(s) 731, 1130, 1225
AcyI GRCGYC 4 cut(s) 357, 363, 1322, 1445
AfaI GTAC 3 cut(s) 66, 301, 1356
AflIII ACRYGT 2 cut(s) 81, 296
AgeI ACCGGT 2 cut(s) 663, 684
AgsI TTSAA 2 cut(s) 627, 763
AjiI CACGTC 1 cut(s) 1293
AjnI CCWGG 4 cut(s) 381, 718, 957, 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AluBI AGCT 3 cut(s) 488, 1203, 1231
AluI AGCT 3 cut(s) 488, 1203, 1231
Alw21I GWGCWC 3 cut(s) 214, 684, 779
Alw26I GTCTC 5 cut(s) 100, 352, 418, 940, 1176
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 4 cut(s) 383, 600, 824, 970
Ama87I CYCGRG 3 cut(s) 1275, 1334, 1348
AoxI GGCC 8 cut(s) 177, 721, 731, 807, 1130, 1225, 1299, 1307
ApaLI GTGCAC 1 cut(s) 680
ApeKI GCWGC 3 cut(s) 132, 317, 1263
AseI ATTAAT 1 cut(s) 885
AsiGI ACCGGT 2 cut(s) 663, 684
Asp700I GAANNNNTTC 1 cut(s) 1284
AspS9I GGNCC 5 cut(s) 645, 666, 794, 1299, 1307
AsuC2I CCSGG 8 cut(s) 221, 735, 1027, 1335, 1336, 1349, 1350, 1421
AvaI CYCGRG 3 cut(s) 1275, 1334, 1348
AvaII GGWCC 3 cut(s) 645, 666, 794
BaeGI GKGCMC 2 cut(s) 684, 1078
BanI GGYRCC 2 cut(s) 994, 1075
BanII GRGCYC 4 cut(s) 556, 910, 1319, 1343
BauI CACGAG 2 cut(s) 97, 213
BbsI GAAGAC 3 cut(s) 246, 919, 1446
Bbv12I GWGCWC 3 cut(s) 214, 684, 779
BbvCI CCTCAGC 1 cut(s) 1187
BbvI GCAGC 3 cut(s) 119, 304, 1275
BccI CCATC 4 cut(s) 102, 443, 611, 1551
BceAI ACGGC 3 cut(s) 43, 309, 1117
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 4 cut(s) 383, 720, 959, 1083
BcnI CCSGG 8 cut(s) 221, 735, 1027, 1335, 1336, 1349, 1350, 1421
BcoDI GTCTC 5 cut(s) 100, 352, 418, 940, 1176
BfaI CTAG 4 cut(s) 483, 932, 1200, 1542
BfoI RGCGCY 2 cut(s) 753, 1564
BfuAI ACCTGC 2 cut(s) 137, 1266
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 7 cut(s) 133, 230, 318, 1225, 1264, 1416, 1443
BlsI GCNGC 7 cut(s) 134, 231, 319, 1226, 1265, 1417, 1444
Bme18I GGWCC 3 cut(s) 645, 666, 794
BmeT110I CYCGRG 3 cut(s) 1275, 1334, 1348
BmgBI CACGTC 1 cut(s) 1293
BmgT120I GGNCC 5 cut(s) 645, 666, 794, 1299, 1307
BmiI GGNNCC 8 cut(s) 35, 996, 1024, 1077, 1092, 1300, 1340, 1390
BmsI GCATC 4 cut(s) 56, 628, 940, 1511
BpiI GAAGAC 3 cut(s) 246, 919, 1446
BplI GAGNNNNNCTC 2 cut(s) 218, 250
Bpu10I CCTNAGC 1 cut(s) 1187
BpuMI CCSGG 8 cut(s) 221, 735, 1027, 1335, 1336, 1349, 1350, 1421
Bsa29I ATCGAT 1 cut(s) 1505
BsaAI YACGTR 1 cut(s) 495
BsaHI GRCGYC 4 cut(s) 357, 363, 1322, 1445
BsaI GGTCTC 1 cut(s) 1176
BsaJI CCNNGG 9 cut(s) 648, 870, 958, 1026, 1222, 1334, 1348, 1384, 1419
BsaWI WCCGGW 2 cut(s) 663, 684
Bse118I RCCGGY 8 cut(s) 175, 184, 663, 684, 705, 991, 1301, 1367
Bse1I ACTGG 1 cut(s) 396
BseBI CCWGG 4 cut(s) 383, 720, 959, 1083
BseCI ATCGAT 1 cut(s) 1505
BseDI CCNNGG 9 cut(s) 648, 870, 958, 1026, 1222, 1334, 1348, 1384, 1419
BseGI GGATG 5 cut(s) 113, 880, 976, 1215, 1562
BseMII CTCAG 1 cut(s) 1201
BseNI ACTGG 1 cut(s) 396
BsePI GCGCGC 3 cut(s) 1405, 1432, 1434
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 684, 1078
BseXI GCAGC 3 cut(s) 119, 304, 1275
Bsh1285I CGRYCG 6 cut(s) 270, 278, 306, 332, 664, 689
BshFI GGCC 8 cut(s) 179, 723, 733, 809, 1132, 1227, 1301, 1309
BshNI GGYRCC 2 cut(s) 994, 1075
BshTI ACCGGT 2 cut(s) 663, 684
BshVI ATCGAT 1 cut(s) 1505
BsiEI CGRYCG 6 cut(s) 270, 278, 306, 332, 664, 689
BsiHKAI GWGCWC 3 cut(s) 214, 684, 779
BsiHKCI CYCGRG 3 cut(s) 1275, 1334, 1348
BsiWI CGTACG 1 cut(s) 299
BslFI GGGAC 7 cut(s) 143, 203, 535, 681, 851, 1255, 1309
BsmAI GTCTC 5 cut(s) 100, 352, 418, 940, 1176
BsmBI CGTCTC 2 cut(s) 100, 352
BsmFI GGGAC 7 cut(s) 143, 203, 535, 681, 851, 1255, 1309
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 8 cut(s) 179, 723, 733, 809, 1132, 1227, 1301, 1309
Bso31I GGTCTC 1 cut(s) 1176
BsoBI CYCGRG 3 cut(s) 1275, 1334, 1348
Bsp1286I GDGCHC 8 cut(s) 214, 556, 684, 779, 910, 1078, 1319, 1343
Bsp143I GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
BspANI GGCC 8 cut(s) 179, 723, 733, 809, 1132, 1227, 1301, 1309
BspCNI CTCAG 1 cut(s) 1200
BspDI ATCGAT 1 cut(s) 1505
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 8 cut(s) 35, 996, 1024, 1077, 1092, 1300, 1340, 1390
BspMI ACCTGC 2 cut(s) 137, 1266
BspPI GGATC 4 cut(s) 383, 600, 824, 970
BspT107I GGYRCC 2 cut(s) 994, 1075
BspTNI GGTCTC 1 cut(s) 1176
BsrBI CCGCTC 1 cut(s) 1345
BsrFI RCCGGY 8 cut(s) 175, 184, 663, 684, 705, 991, 1301, 1367
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 8 cut(s) 175, 184, 663, 684, 705, 991, 1301, 1367
BssECI CCNNGG 9 cut(s) 648, 870, 958, 1026, 1222, 1334, 1348, 1384, 1419
BssHII GCGCGC 3 cut(s) 1405, 1432, 1434
BssMI GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
BssNI GRCGYC 4 cut(s) 357, 363, 1322, 1445
BssSI CACGAG 2 cut(s) 97, 213
BssT1I CCWWGG 3 cut(s) 648, 870, 1384
Bst2BI CACGAG 2 cut(s) 97, 213
Bst2UI CCWGG 4 cut(s) 383, 720, 959, 1083
Bst4CI ACNGT 7 cut(s) 56, 307, 333, 473, 842, 928, 1429
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 4 cut(s) 357, 363, 1322, 1445
BstAPI GCANNNNNTGC 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 495
BstDEI CTNAG 3 cut(s) 1138, 1187, 1235
BstDSI CCRYGG 1 cut(s) 1222
BstF5I GGATG 5 cut(s) 113, 880, 976, 1215, 1562
BstH2I RGCGCY 2 cut(s) 753, 1564
BstKTI GATC 7 cut(s) 270, 278, 391, 595, 819, 920, 965
BstMAI GTCTC 5 cut(s) 100, 352, 418, 940, 1176
BstMBI GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
BstMCI CGRYCG 6 cut(s) 270, 278, 306, 332, 664, 689
BstMWI GCNNNNNNNGC 9 cut(s) 181, 185, 238, 317, 556, 616, 749, 1415, 1442
BstNI CCWGG 4 cut(s) 383, 720, 959, 1083
BstSLI GKGCMC 2 cut(s) 684, 1078
BstV1I GCAGC 3 cut(s) 119, 304, 1275
BstV2I GAAGAC 3 cut(s) 246, 919, 1446
BstXI CCANNNNNNTGG 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 1505
BsuRI GGCC 8 cut(s) 179, 723, 733, 809, 1132, 1227, 1301, 1309
BsuTUI ATCGAT 1 cut(s) 1505
BtgI CCRYGG 1 cut(s) 1222
BtrI CACGTC 1 cut(s) 1293
BtsCI GGATG 5 cut(s) 113, 880, 976, 1215, 1562
BveI ACCTGC 2 cut(s) 137, 1266
CciI TCATGA 1 cut(s) 577
Cfr10I RCCGGY 8 cut(s) 175, 184, 663, 684, 705, 991, 1301, 1367
Cfr13I GGNCC 5 cut(s) 645, 666, 794, 1299, 1307
Cfr42I CCGCGG 1 cut(s) 1225
Cfr9I CCCGGG 2 cut(s) 1334, 1348
ClaI ATCGAT 1 cut(s) 1505
CpoI CGGWCCG 1 cut(s) 666
CsiI ACCWGGT 1 cut(s) 381
Csp6I GTAC 3 cut(s) 65, 300, 1355
CspAI ACCGGT 2 cut(s) 663, 684
CspI CGGWCCG 1 cut(s) 666
CviAII CATG 3 cut(s) 429, 578, 1128
CviQI GTAC 3 cut(s) 65, 300, 1355
DdeI CTNAG 3 cut(s) 1138, 1187, 1235
DpnI GATC 7 cut(s) 269, 277, 390, 594, 818, 919, 964
DpnII GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
DrdI GACNNNNNNGTC 2 cut(s) 362, 1249
DseDI GACNNNNNNGTC 2 cut(s) 362, 1249
EaeI YGGCCR 3 cut(s) 731, 1130, 1225
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 3 cut(s) 658, 978, 983
Eco130I CCWWGG 3 cut(s) 648, 870, 1384
Eco24I GRGCYC 4 cut(s) 556, 910, 1319, 1343
Eco31I GGTCTC 1 cut(s) 1176
Eco32I GATATC 1 cut(s) 1489
Eco47I GGWCC 3 cut(s) 645, 666, 794
Eco88I CYCGRG 3 cut(s) 1275, 1334, 1348
EcoRII CCWGG 4 cut(s) 381, 718, 957, 1081
EcoRV GATATC 1 cut(s) 1489
EcoT14I CCWWGG 3 cut(s) 648, 870, 1384
EcoT22I ATGCAT 1 cut(s) 434
EcoT38I GRGCYC 4 cut(s) 556, 910, 1319, 1343
ErhI CCWWGG 3 cut(s) 648, 870, 1384
Esp3I CGTCTC 2 cut(s) 100, 352
FaeI CATG 3 cut(s) 432, 581, 1131
FaqI GGGAC 7 cut(s) 143, 203, 535, 681, 851, 1255, 1309
FatI CATG 3 cut(s) 428, 577, 1127
FauI CCCGC 9 cut(s) 77, 181, 234, 322, 543, 1298, 1350, 1420, 1431
FauNDI CATATG 1 cut(s) 405
FblI GTMKAC 4 cut(s) 328, 545, 660, 844
Fnu4HI GCNGC 7 cut(s) 133, 230, 318, 1225, 1264, 1416, 1443
FokI GGATG 4 cut(s) 120, 887, 983, 1202
FriOI GRGCYC 4 cut(s) 556, 910, 1319, 1343
Fsp4HI GCNGC 7 cut(s) 133, 230, 318, 1225, 1264, 1416, 1443
FspBI CTAG 4 cut(s) 483, 932, 1200, 1542
FspI TGCGCA 2 cut(s) 103, 1262
GluI GCNGC 7 cut(s) 133, 230, 318, 1225, 1264, 1416, 1443
HaeII RGCGCY 2 cut(s) 753, 1564
HaeIII GGCC 8 cut(s) 179, 723, 733, 809, 1132, 1227, 1301, 1309
Hin1I GRCGYC 4 cut(s) 357, 363, 1322, 1445
Hin1II CATG 3 cut(s) 432, 581, 1131
HincII GTYRAC 6 cut(s) 329, 546, 661, 845, 1174, 1539
HindII GTYRAC 6 cut(s) 329, 546, 661, 845, 1174, 1539
HinfI GANTC 6 cut(s) 90, 574, 985, 1109, 1243, 1473
HpaI GTTAAC 1 cut(s) 1174
Hpy166II GTNNAC 7 cut(s) 329, 546, 661, 682, 845, 1174, 1539
Hpy188I TCNGA 6 cut(s) 267, 275, 466, 570, 821, 922
Hpy188III TCNNGA 8 cut(s) 87, 258, 483, 515, 578, 814, 890, 1106
Hpy8I GTNNAC 7 cut(s) 329, 546, 661, 682, 845, 1174, 1539
HpyAV CCTTC 6 cut(s) 131, 512, 709, 820, 1065, 1320
HpyCH4III ACNGT 7 cut(s) 56, 307, 333, 473, 842, 928, 1429
HpyCH4IV ACGT 5 cut(s) 143, 363, 494, 1292, 1515
HpyCH4V TGCA 4 cut(s) 432, 498, 682, 1127
HpyF10VI GCNNNNNNNGC 9 cut(s) 181, 185, 238, 317, 556, 616, 749, 1415, 1442
HpyF3I CTNAG 3 cut(s) 1138, 1187, 1235
HpySE526I ACGT 5 cut(s) 143, 363, 494, 1292, 1515
Hsp92I GRCGYC 4 cut(s) 357, 363, 1322, 1445
Hsp92II CATG 3 cut(s) 432, 581, 1131
KroI GCCGGC 5 cut(s) 175, 184, 705, 991, 1301
KroNI GCCGGC 5 cut(s) 177, 186, 707, 993, 1303
KspAI GTTAAC 1 cut(s) 1174
KspI CCGCGG 1 cut(s) 1225
Kzo9I GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
LmnI GCTCC 7 cut(s) 39, 721, 1090, 1148, 1338, 1350, 1414
Lsp1109I GCAGC 3 cut(s) 119, 304, 1275
LweI GCATC 4 cut(s) 56, 628, 940, 1511
MabI ACCWGGT 1 cut(s) 381
MaeI CTAG 4 cut(s) 483, 932, 1200, 1542
MaeII ACGT 5 cut(s) 143, 363, 494, 1292, 1515
MaeIII GTNAC 2 cut(s) 754, 973
MalI GATC 7 cut(s) 269, 277, 390, 594, 818, 919, 964
MauBI CGCGCGCG 1 cut(s) 1434
MbiI CCGCTC 1 cut(s) 1345
MboI GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
MboII GAAGA 6 cut(s) 246, 380, 775, 924, 1446, 1522
MhlI GDGCHC 8 cut(s) 214, 556, 684, 779, 910, 1078, 1319, 1343
MluCI AATT 6 cut(s) 10, 478, 727, 767, 1062, 1165
MluI ACGCGT 2 cut(s) 81, 296
MlyI GAGTC 3 cut(s) 979, 1237, 1467
MmeI TCCRAC 1 cut(s) 623
Mph1103I ATGCAT 1 cut(s) 434
MreI CGCCGGCG 1 cut(s) 184
MroNI GCCGGC 5 cut(s) 175, 184, 705, 991, 1301
MroXI GAANNNNTTC 1 cut(s) 1284
MseI TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
MspA1I CMGCKG 2 cut(s) 317, 1224
Mva1269I GAATGC 1 cut(s) 380
MvaI CCWGG 4 cut(s) 383, 720, 959, 1083
MwoI GCNNNNNNNGC 9 cut(s) 181, 185, 238, 317, 556, 616, 749, 1415, 1442
NaeI GCCGGC 5 cut(s) 177, 186, 707, 993, 1303
NciI CCSGG 8 cut(s) 221, 735, 1027, 1335, 1336, 1349, 1350, 1421
NdeI CATATG 1 cut(s) 405
NdeII GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
NgoMIV GCCGGC 5 cut(s) 175, 184, 705, 991, 1301
NlaIII CATG 3 cut(s) 432, 581, 1131
NlaIV GGNNCC 8 cut(s) 35, 996, 1024, 1077, 1092, 1300, 1340, 1390
NmeAIII GCCGAG 3 cut(s) 1298, 1457, 1472
NsbI TGCGCA 2 cut(s) 103, 1262
NsiI ATGCAT 1 cut(s) 434
PagI TCATGA 1 cut(s) 577
PauI GCGCGC 3 cut(s) 1405, 1432, 1434
PcsI WCGNNNNNNNCGW 4 cut(s) 295, 299, 363, 1319
PctI GAATGC 1 cut(s) 380
PdiI GCCGGC 5 cut(s) 177, 186, 707, 993, 1303
PdmI GAANNNNTTC 1 cut(s) 1284
PfeI GAWTC 3 cut(s) 90, 574, 1109
Pfl23II CGTACG 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 663, 684
PkrI GCNGC 7 cut(s) 134, 231, 319, 1226, 1265, 1417, 1444
Ple19I CGATCG 2 cut(s) 270, 278
PleI GAGTC 3 cut(s) 979, 1237, 1467
PpsI GAGTC 3 cut(s) 979, 1237, 1467
Ppu21I YACGTR 1 cut(s) 495
PshBI ATTAAT 1 cut(s) 885
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 4 cut(s) 381, 718, 957, 1081
PspGI CCWGG 4 cut(s) 381, 718, 957, 1081
PspLI CGTACG 1 cut(s) 299
PspN4I GGNNCC 8 cut(s) 35, 996, 1024, 1077, 1092, 1300, 1340, 1390
PspPI GGNCC 5 cut(s) 645, 666, 794, 1299, 1307
PteI GCGCGC 3 cut(s) 1405, 1432, 1434
PvuI CGATCG 2 cut(s) 270, 278
RsaI GTAC 3 cut(s) 66, 301, 1356
RsaNI GTAC 3 cut(s) 65, 300, 1355
Rsr2I CGGWCCG 1 cut(s) 666
RsrII CGGWCCG 1 cut(s) 666
SacII CCGCGG 1 cut(s) 1225
SalI GTCGAC 4 cut(s) 327, 544, 659, 843
SaqAI TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
SatI GCNGC 7 cut(s) 133, 230, 318, 1225, 1264, 1416, 1443
Sau3AI GATC 7 cut(s) 267, 275, 388, 592, 816, 917, 962
Sau96I GGNCC 5 cut(s) 645, 666, 794, 1299, 1307
SchI GAGTC 3 cut(s) 979, 1237, 1467
SduI GDGCHC 8 cut(s) 214, 556, 684, 779, 910, 1078, 1319, 1343
SexAI ACCWGGT 1 cut(s) 381
SfaNI GCATC 4 cut(s) 56, 628, 940, 1511
Sfr303I CCGCGG 1 cut(s) 1225
SgrAI CRCCGGYG 2 cut(s) 184, 1367
SgrBI CCGCGG 1 cut(s) 1225
SinI GGWCC 3 cut(s) 645, 666, 794
SmaI CCCGGG 2 cut(s) 1336, 1350
Sse9I AATT 6 cut(s) 10, 478, 727, 767, 1062, 1165
SspMI CTAG 4 cut(s) 483, 932, 1200, 1542
StyI CCWWGG 3 cut(s) 648, 870, 1384
TaaI ACNGT 7 cut(s) 56, 307, 333, 473, 842, 928, 1429
TaiI ACGT 5 cut(s) 146, 366, 497, 1295, 1518
TaqII GACCGA 1 cut(s) 782
TasI AATT 6 cut(s) 10, 478, 727, 767, 1062, 1165
TauI GCSGC 4 cut(s) 232, 1227, 1418, 1445
TfiI GAWTC 3 cut(s) 90, 574, 1109
Tru1I TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
Tru9I TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
TseI GCWGC 3 cut(s) 132, 317, 1263
TspDTI ATGAA 4 cut(s) 64, 566, 871, 1523
TspGWI ACGGA 6 cut(s) 26, 334, 605, 1083, 1159, 1499
TspMI CCCGGG 2 cut(s) 1334, 1348
VneI GTGCAC 1 cut(s) 680
VpaK11BI GGWCC 3 cut(s) 645, 666, 794
VspI ATTAAT 1 cut(s) 885
XbaI TCTAGA 1 cut(s) 482
XmaI CCCGGG 2 cut(s) 1334, 1348
XmiI GTMKAC 4 cut(s) 328, 545, 660, 844
XmnI GAANNNNTTC 1 cut(s) 1284
XspI CTAG 4 cut(s) 483, 932, 1200, 1542
ZraI GACGTC 1 cut(s) 364
Zsp2I ATGCAT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.